{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T17:32:57Z","timestamp":1786469577500,"version":"build-2736575974"},"reference-count":52,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"United States Department of Agriculture and the National Institute of Food and Agriculture","award":["2023-38821-39979"],"award-info":[{"award-number":["2023-38821-39979"]}]},{"award":["2023-38821-39979"],"award-info":[{"award-number":["2023-38821-39979"]}],"id":[{"id":"https:\/\/ror.org\/05qx3fv49","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Contamination of retail foods by Listeria species, compounded by emerging antimicrobial resistance (AMR), presents a critical public health challenge that demands continuous surveillance. This study evaluated prevalence, species distribution, and AMR profiles of Listeria spp. isolated from 861 retail food samples in North Carolina, USA, (2018 and 2020). Isolation and molecular characterization were achieved through selective culturing, multiplex PCR, and antimicrobial susceptibility profiling via disk diffusion. Listeria was detected in 8.48% (73\/861) samples, with 68 isolates identified to species level. Non-pathogenic strains predominated, led by L. welshimeri (24.66% of isolates) and L. innocua (21.92%). Pathogenic species (L. monocytogenes; L. ivanovii) represented 17.81% of isolates and were recovered exclusively from raw commodities. Vegetables yielded the most positive samples overall, but raw milk showed the highest individual category prevalence. Processed foods remained largely uncontaminated. Unprocessed foods were 27 times more likely to harbor Listeria than processed alternatives (RR = 27.19; 95% CI: 3.84\u2013192.51. p &lt; 0.0001). Phenotypic resistance was most frequent to Penicillin G (33.82%). Multidrug resistance (MDR) was identified in 64.71% of isolates, encompassing 84.62% of pathogenic and 60.00% of non-pathogenic species. Raw agricultural commodities serve as critical reservoirs for resistant Listeria, emphasizing the need for integrated surveillance frameworks.<\/jats:p>","DOI":"10.3390\/microorganisms14081769","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T16:30:32Z","timestamp":1786465832000},"page":"1769","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Occurrence, Species Distribution, and Antimicrobial Resistance of Listeria spp. in Foods from Retail and Farm Outlets in North Carolina, USA"],"prefix":"10.3390","volume":"14","author":[{"given":"Akbar","family":"Bahrami","sequence":"first","affiliation":[{"name":"Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, North Carolina Research Campus, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shurrita","family":"Davis","sequence":"additional","affiliation":[{"name":"Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, North Carolina Research Campus, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erute Magdalene","family":"Adongbede","sequence":"additional","affiliation":[{"name":"Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, North Carolina Research Campus, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janak Raj","family":"Khatiwada","sequence":"additional","affiliation":[{"name":"Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, North Carolina Research Campus, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7826-2711","authenticated-orcid":false,"given":"Rishipal Rastrapal","family":"Bansode","sequence":"additional","affiliation":[{"name":"Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, North Carolina Research Campus, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0886-5204","authenticated-orcid":false,"given":"Leonard Lamont","family":"Williams","sequence":"additional","affiliation":[{"name":"Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, North Carolina Research Campus, Kannapolis, NC 28081, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.2147\/IJGM.S496799","article-title":"Epidemiological Characteristics of Foodborne Disease Outbreaks in a Hospital: A 5-Year Retrospective Study","volume":"18","author":"Yang","year":"2025","journal-title":"Int. J. Gen. Med."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mohapi, D.A., and Nkhebenyane, S.J. (2025). Listeria monocytogenes and Listeria ivanovii Virulence and Adaptations Associated with Leafy Vegetables from Small-Scale Farm and a Shift of Microbiota to a New Niche at Markets: A Systematic Review. Microorganisms, 14.","DOI":"10.3390\/microorganisms14010076"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"537","DOI":"10.2478\/jvetres-2023-0068","article-title":"Why does Listeria monocytogenes survive in food and food-production environments?","volume":"67","author":"Osek","year":"2023","journal-title":"J. Vet. Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Mazaheri, T., Cervantes-Huaman, B.R.H., Bermudez-Caodevila, M., Ripolles-Avila, C., and Rodriguez-Jerez, J.J. (2021). Listeria monocytogenes Biofilms in the Food Industry: Is the Current Hygiene Program Sufficient to Combat the Persistence of the Pathogen?. Microorganisms, 9.","DOI":"10.3390\/microorganisms9010181"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Manyi-Loh, C.E., and Lues, R. (2025). Listeria monocytogenes and Listeriosis: The Global Enigma. Foods, 14.","DOI":"10.3390\/foods14071266"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e00938-23","DOI":"10.1128\/mbio.00938-23","article-title":"Taxonomy, ecology, and relevance to food safety of the genus Listeria with a particular consideration of new Listeria species described between 2010 and 2022","volume":"15","author":"Orsi","year":"2023","journal-title":"MBio"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1139\/m95-076","article-title":"Repression of motility and flagellin production at 37 degrees C is stronger in Listeria monocytogenes than in the nonpathogenic species Listeria innocua","volume":"41","author":"Kathariou","year":"1995","journal-title":"Can. J. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"104215","DOI":"10.1016\/j.resmic.2024.104215","article-title":"Listeria monocytogenes in beef: A hidden risk","volume":"175","author":"Reis","year":"2024","journal-title":"Res. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.1080\/21505594.2021.1975526","article-title":"Pathogenicity and virulence of Listeria monocytogenes: A trip from environmental to medical microbiology","volume":"12","author":"Quereda","year":"2021","journal-title":"Virulence"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"104750","DOI":"10.1016\/j.tifs.2024.104750","article-title":"Mapping the landscape of listeriosis outbreaks (1998\u20132023): Trends, challenges, and regulatory responses in the United States","volume":"154","author":"Su","year":"2024","journal-title":"Trends Food Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"D\u2019Ambrosio, G., Schirone, M., and Paparella, A. (2026). Listeria monocytogenes in Ready-to-Eat Foods: Risk Perspectives Across Different Regulatory Systems. Foods, 15.","DOI":"10.3390\/foods15030470"},{"key":"ref_12","first-page":"669","article-title":"Foodborne Illness Acquired in the United States\u2014Major Pathogens, 2019","volume":"31","author":"Scallan","year":"2025","journal-title":"Emerg. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5273","DOI":"10.1007\/s00253-016-7552-2","article-title":"Characteristics and distribution of Listeria spp., including Listeria species newly described since 2009","volume":"100","author":"Orsi","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Zhu, Q., Gooneratne, R., and Hussain, M.A. (2017). Listeria monocytogenes in Fresh Produce: Outbreaks, Prevalence and Contamination Levels. Foods, 6.","DOI":"10.3390\/foods6030021"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Law, J.W.-F., Mutalib, N.-S.A., Chan, K.-G., and Lee, L.-H. (2015). An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.01227"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Li, F., Ye, Q., Chen, M., Zhang, J., Xue, L., Wang, J., Wu, S., Zeng, H., Gu, Q., and Zhang, Y. (2021). Multiplex PCR for the Identification of Pathogenic Listeria in Flammulina velutipes Plant Based on Novel Specific Targets Revealed by Pan-Genome Analysis. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.634255"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Soltysiuk, M., Przyborowska, P., Wiszniewska-Laszczch, A., and Tobolski, D. (2025). Prevalence and antimicrobial resistance profile of Listeria spp. isolated from raw fish. BMC Vet. Res., 21.","DOI":"10.1186\/s12917-025-04792-y"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s42770-025-01853-0","article-title":"Exploring the prevalence and antibiotic resistance of Listeria of Listeria monocytogenes in diverse food commodities across Sikkim, India","volume":"57","author":"Byathut","year":"2026","journal-title":"Braz. J. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zawiasa, A., and Olejnik-Schmidt, A. (2025). Antibiotic Resistance Profiles and Genetic Determinants of Listeria innocua Isolated from Food Sources in Poland. Genes, 16.","DOI":"10.3390\/genes16121455"},{"key":"ref_20","first-page":"5498","article-title":"Identification of Listeria spp. strains isolated from meat products and meat production plants by multiplex polymerase chain reaction","volume":"5","author":"Mazza","year":"2016","journal-title":"Ital. J. Food Saf."},{"key":"ref_21","unstructured":"Clinical and Laboratory Standards Instititute (CLSI) (2016). Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute (CLSI). [26th ed.]. CLSI Supplement M100-S26."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Vatopoulos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Jagadeesan, B., Baert, L., Wiedmann, M., and Orsi, R.H. (2019). Comparative Analysis of Tools and Approaches for Source Tracking Listeria monocytogenes in a Food Facility Using Whole-Genome Sequence Data. Front. Moicrobiol., 10.","DOI":"10.3389\/fmicb.2019.00947"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e01177-22","DOI":"10.1128\/aem.01177-22","article-title":"Whole-Genome Sequencing-Based Characterization of Listeria Isolates from Produce Packinghouses and Fresh-Cut Facilities Suggests Both Persistence and Reintroduction of Fully Virulent L. monocytogenes","volume":"88","author":"Sullivan","year":"2022","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.4315\/0362-028X.JFP-14-183","article-title":"Listeria monocytogenes and Listeria spp. Contamination Patterns in Retail Delicatessen Establishments in Three U.S. States","volume":"77","author":"Simmons","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.ijfoodmicro.2016.08.032","article-title":"Characterization of nonpathogenic Listeria species isolated from food and food processing environment","volume":"238","author":"Korsak","year":"2016","journal-title":"Int. J. Food Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"107173","DOI":"10.1016\/j.foodcont.2020.107173","article-title":"Listeria monocytogenes is prevalent in retail produce environments but Salmonella enterica is rare","volume":"113","author":"Burnett","year":"2020","journal-title":"Food Control"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"101173","DOI":"10.1016\/j.onehlt.2025.101173","article-title":"Listeria monocytogenes in organic and conventional farming: Epidemiology, risks, and solutions within a One Health framework","volume":"21","author":"Ryzhova","year":"2025","journal-title":"One Health"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Schoder, D., Pelz, A., and Paulsen, P. (2023). Transmission Scenarios of Listeria monocytogenes on Small Ruminant On-Farm Dairies. Foods, 12.","DOI":"10.3390\/foods12020265"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1089\/fpd.2024.0029","article-title":"Prevalence of Listeria monocytogenes in Milk and Dairy Product Supply Chains: A Global Systematic Review and Meta-analysis","volume":"21","author":"Li","year":"2024","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5643478","DOI":"10.1155\/2022\/5643478","article-title":"Listeria Species Occurrence and Associated Risk Factors and Antibiogram of Listeria Monocytogenes in Milk and Milk Products in Ambo, Holeta, and Bako Towns, Oromia Regional State, Ethiopia","volume":"2022","author":"Borena","year":"2022","journal-title":"Vet. Med. Int."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"100014","DOI":"10.1016\/j.jfp.2022.11.006","article-title":"Prevalence of Listeria monocytogenes, Salmonella spp., Shiga toxin-producing Escherichia coli, and Campylobacter spp. in raw milk in the United States between 2000 and 2019: A systematic review and meta-analysis","volume":"86","author":"Williams","year":"2023","journal-title":"J. Food Prot."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1080\/22221751.2021.1888658","article-title":"Prevalence and transmission characteristics of Listeria species from ruminants in farm and slaughtering environments in China","volume":"10","author":"Zhao","year":"2021","journal-title":"Emerg. Microbes Infect."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e1255","DOI":"10.1002\/mbo3.1255","article-title":"Plants as a realized niche for Listeria monocytogenes","volume":"10","author":"Troung","year":"2021","journal-title":"MicrobiologyOpen"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.4315\/JFP-20-094","article-title":"Detection and Prevalence of Listeria in U.S. Produce Packinghouses and Fresh-Cut Facilities","volume":"83","author":"Sullivan","year":"2020","journal-title":"J. Food Prot."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.4315\/0362-028X.JFP-14-584","article-title":"Irrigation Is Significantly Associated with an Increased Prevalence of Listeria monocytogenes in Produce Production Environments in New York State","volume":"78","author":"Weller","year":"2015","journal-title":"J. Food Prot."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Shaaban, S.I., Awwad, N., Nossair, M., Ayoub, M., and El-Majeed Eid, A. (2025). Gene profiling and serotyping of multidrug-resistant Listeria monocytogenes isolated from humans, animals and dairy products. BMC Vet. Res., 21.","DOI":"10.1186\/s12917-025-05138-4"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Zhang, H., Luo, X., Aspridou, Z., Misiou, O., Dong, P., and Zhang, Y. (2023). The Prevalence and Antibiotic-Resistant of Listeria monocytogenes in Livestock and Poultry Meat in China and the EU from 2001 to 2022: A Systematic Review and Meta-Analysis. Foods, 12.","DOI":"10.3390\/foods12040769"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.4102\/ojvr.v87i1.1869","article-title":"A review of Listeria monocytogenes from meat and meat products: Epidemiology, virulence factors, antimicrobial resistance and diagnosis","volume":"87","author":"Matle","year":"2020","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"104778","DOI":"10.1016\/j.fm.2025.104778","article-title":"Survival of Listeria monocytogenes on growing and harvested Trumpet Royale (Pleurotus eryngii), Alba Clamshell (Hypsizygus tessellatus), and Brown Clamshell (Hypsizygus tessellatus) mushrooms","volume":"130","author":"Tu","year":"2025","journal-title":"Food Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"608","DOI":"10.4315\/0362-028X.JFP-12-292","article-title":"Incidence of Listeria monocytogenes and Listeria spp. in a Small-Scale Mushroom Production Facility","volume":"76","author":"Viswanath","year":"2013","journal-title":"J. Food Prot."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Townsend, A., Strawn, L.K., Chapman, B.J., and Dunn, L.L. (2021). A Systematic Review of Listeria Species and Listeria monocytogenes Prevalence, Persistence, and Diversity throughout the Fresh Produce Supply Chain. Foods, 10.","DOI":"10.3390\/foods10061427"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"277","DOI":"10.4315\/0362-028X.JFP-19-411","article-title":"Prevalence, Persistence, and Diversity of Listeria monocytogenes and Listeria Species in Produce Packinghouses in Three U.S. States","volume":"83","author":"Estrada","year":"2020","journal-title":"J. Food Prot."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Nikolaou, F.G., Colobatiu, L.M., Ciupescu, L.M., Tabaran, A., Hategan, A.R., Mihaiu, R., Tanassuica, R., Poenaru, M.M., and Mihaiu, M. (2025). Prevalence and Antimicrobial Resistance of Listeria monocytogenes Isolated from Dairy Products in Romania. Antibiotics, 14.","DOI":"10.3390\/antibiotics14050482"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"100767","DOI":"10.1016\/j.jfp.2026.100767","article-title":"Antimicrobial-Resistant Listeria Species Isolated from Beef and Beef Products Sold in Mpumalanga Province, South Africa","volume":"89","author":"Moabelo","year":"2026","journal-title":"J. Food Prot."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Kiss, R., Marosi, B., Petrik, B., Lakatos, B., and Szabo, B.G. (2024). Clinical and microbiological characteristics and follow-up of invasive Listeria monocytogenes infection among hospitalized patients: Real-world experience of 16 years from Hungary. BMC Microbiol., 24.","DOI":"10.1186\/s12866-024-03478-z"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Kozytska, T., Neubauer, H., and Wareth, G. (2026). Listeria ivanovii-an underestimated pathogen in veterinary medicine. Front. Vet. Sci., 13.","DOI":"10.3389\/fvets.2026.1844936"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3101","DOI":"10.3390\/ijerph9093101","article-title":"Current Status of Antibiograms of Listeria ivanovii and Enterobacter cloacae Isolated from Ready-to-Eat Foods in Alice, South Africa","volume":"9","author":"Nyenje","year":"2012","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1002\/mbo3.155","article-title":"Antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental Listeria spp. isolates including Listeria monocytogenes","volume":"3","author":"Bertsch","year":"2014","journal-title":"MicrobiologyOpen"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"100127","DOI":"10.1016\/j.medmic.2025.100127","article-title":"The past, present, future of Listeria monocytogenes: Understanding the molecular pathways, antibiotic resistance and public health implications","volume":"25","author":"Anupama","year":"2025","journal-title":"Med. Microecol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Kayode, A.J., and Okoh, A.I. (2022). Assessment of multidrug-resistant Listeria monocytogenes in milk and milk product and One Health perspective. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0270993"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Wartha, S., Bretschneider, N., Dangel, A., Hobmaier, B., Hormansdorfer, S., Huber, I., Murr, L., Pavlovic, M., Sprenger, A., and Wenning, M. (2023). Genetic Characterization of Listeria from Food of Non-Animal Origin Products and from Producing and Processing Companies in Bavaria, Germany. Foods, 12.","DOI":"10.3390\/foods12061120"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1769\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T16:37:49Z","timestamp":1786466269000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1769"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,11]]},"references-count":52,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["microorganisms14081769"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14081769","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,11]]}}}