{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T04:30:41Z","timestamp":1786681841154,"version":"build-2736575974"},"reference-count":97,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"crossref","award":["ORF-2026-227"],"award-info":[{"award-number":["ORF-2026-227"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"crossref"}]},{"award":["ORF-2026-227"],"award-info":[{"award-number":["ORF-2026-227"]}],"id":[{"id":"https:\/\/ror.org\/02f81g417","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Endophytic fungi are promising sources of chemically diverse metabolites. Fusarium species exhibit diverse biological roles, ranging from beneficial applications to detrimental effects, including plant diseases and mycotoxin production. A few reports have investigated Fusarium equiseti bioactivity, but no reports have evaluated its anti-inflammatory potential. In the present study, the ethyl acetate (EtOAc) extract obtained from an endophytic F. equiseti, isolated for the first time from the bulb of Pancratium maritimum L., was evaluated for its in vitro antioxidant, cytotoxic, anti-acetyl- and butyrylcholinesterases, and anti-inflammatory activities. The extract was chemically profiled by LC-MS\/MS, evaluated in complementary antioxidant and cytotoxicity screens, and assessed for inhibition of nitric oxide (NO) production in lipopolysaccharide-stimulated RAW264.7 macrophages. In addition, putatively annotated metabolites were further examined by molecular docking against inducible nitric oxide synthase (iNOS), as a promising biological activity providing a target directly aligned with the NO-based assay. The EtOAc extract showed total phenolics content of 29.31 mg GAE\/g and a total alkaloid content of 8.43 mg AE\/g, with a DPPH radical scavenging IC50 value of 2.18 mg\/mL. Cytotoxicity assessment against the RAW264.7 cell line revealed an IC50 value of 49.95 \u03bcg\/mL, while the Artemia salina lethality assay yielded an LC50 value of 1.86 mg\/mL. Notably, the extract demonstrated potent anti-inflammatory activity through nitric oxide inhibition, with an IC50 value of 4.70 \u03bcg\/mL compared with 18.46 \u03bcg\/mL for quercetin. LC-MS\/MS analysis offered the tentative identification of 31 metabolites in the extract. Docking against iNOS identified fusarioxazin as the highest-scoring ligand (\u22128.8 kcal\/mol), although these computational predictions require experimental validation with purified compounds. The data identify suppression of macrophage NO production as the principal bioactivity of the crude F. equiseti TU-64 extract. Collectively, these findings highlight the potential of endophytic F. equiseti as a promising source of bioactive metabolites for the development of natural therapeutic agents.<\/jats:p>","DOI":"10.3390\/microorganisms14081773","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T07:57:26Z","timestamp":1786521446000},"page":"1773","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Biological Potential of an Endophytic Fusarium equiseti from Pancratium maritimum Supported by Metabolic Profiling and Molecular Docking"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3845-9701","authenticated-orcid":false,"given":"Amal A.","family":"Al Mousa","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 145111, Riyadh ZIP 4545, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5226-1503","authenticated-orcid":false,"given":"Mohamed E.","family":"Abouelela","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reda A.","family":"Abdelhamid","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy (Boys), Al-Azhar University, Assiut Branch, Assiut 71524, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5687-2305","authenticated-orcid":false,"given":"Hassan","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt"},{"name":"Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed M. M.","family":"Abdelrahem","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0467-9164","authenticated-orcid":false,"given":"Mohamed F.","family":"Awad","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shurouq A.","family":"Fahmy","sequence":"additional","affiliation":[{"name":"Ministry of Health and Populations, Cairo P.O. Box 11516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huda A.","family":"Aljhne","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 145111, Riyadh ZIP 4545, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4793-8528","authenticated-orcid":false,"given":"Nageh F.","family":"Abo-Dahab","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0820-8087","authenticated-orcid":false,"given":"Abdallah M. A.","family":"Hassane","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Youssef, D.T.A., Shaala, L.A., and Altyar, A.E. (2022). Cytotoxic Phenylpropanoid Derivatives and Alkaloids from the Flowers of Pancratium maritimum L.. Plants, 11.","DOI":"10.3390\/plants11040476"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Taher, R.F., Abd El Ghany, E.M., El-Gendy, Z.A., Elghonemy, M.M., Hassan, H.A., Abdel Jaleel, G.A., Hassan, A., Sarker, T.C., Abd-ElGawad, A.M., and Farag, M.A. (2025). In vivo anti-ulceration effect of Pancratium maritimum extract against ethanol-induced rats via NLRP3 inflammasome and HMGB1\/TLR4\/MYD88\/NF-\u03ba\u03b2 signaling pathways and its extract metabolite profile. PLoS ONE, 20.","DOI":"10.1371\/journal.pone.0321018"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.indcrop.2016.09.021","article-title":"Determination of phenolic composition and antioxidant activities of Pancratium maritimum L. from Tunisia","volume":"94","author":"Rokbeni","year":"2016","journal-title":"Ind. Crop Prod."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"9301247","DOI":"10.1155\/2018\/9301247","article-title":"Investigating the Antiproliferative and Antioxidant Properties of Pancratium maritimum L. (Amaryllidaceae) Stems, Flowers, Bulbs, and Fruits Extracts","volume":"2018","author":"Leporini","year":"2018","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Murthy, H.N., Paek, K.Y., and Park, S.Y. (2024). Bioactive Compounds and Biological Activities of the Genus Pancratium. Bioactive Compounds in the Storage Organs of Plants, Springer.","DOI":"10.1007\/978-3-031-44746-4"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e202400407","DOI":"10.1002\/open.202400407","article-title":"Antioxidant, Antimicrobial, Anticancer, and Molecular Docking Insights into Pancratium maritimum Seeds and Flowers: A Phytochemical Approach","volume":"14","author":"Durmaz","year":"2025","journal-title":"ChemistryOpen"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Cicio, A., Sut, S., Dall\u2019Acqua, S., Bruno, M., Luparello, C., Serio, R., and Zizzo, M.G. (2023). Chemical Characterization and Cytotoxic and Antioxidant Activity Evaluation of the Ethanol Extract from the Bulbs of Pancratium maritimun Collected in Sicily. Molecules, 28.","DOI":"10.3390\/molecules28103986"},{"key":"ref_8","first-page":"52","article-title":"Antiproliferative Effects of Pancratium maritimum Extracts on Normal and Cancerous Cells","volume":"43","author":"Tayoub","year":"2018","journal-title":"Iran. J. Med. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1007\/s11426-013-4967-9","article-title":"Multiple biological functions and pharmacological effects of lycorine","volume":"56","author":"Cao","year":"2013","journal-title":"Sci. China Chem."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Di Sotto, A., Valipour, M., Azari, A., Di Giacomo, S., and Irannejad, H. (2023). Benzoindolizidine Alkaloids Tylophorine and Lycorine and Their Analogues with Antiviral, Anti-Inflammatory, and Anticancer Properties: Promises and Challenges. Biomedicines, 11.","DOI":"10.3390\/biomedicines11102619"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1055\/s-0042-115034","article-title":"Narciclasine\u2014An Amaryllidaceae Alkaloid with Potent Antitumor and Anti-Inflammatory Properties","volume":"82","year":"2016","journal-title":"Planta Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s12943-018-0782-4","article-title":"Mechanisms of receptor tyrosine kinase activation in cancer","volume":"17","author":"Du","year":"2018","journal-title":"Mol. Cancer"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1021\/acs.jnatprod.9b01285","article-title":"Natural Products as Sources of New Drugs over the Nearly Four Decades from 01\/1981 to 09\/2019","volume":"83","author":"Newman","year":"2020","journal-title":"J. Nat. Prod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"107337","DOI":"10.1016\/j.biotechadv.2019.01.004","article-title":"Current Status and Contemporary Approaches to the Discovery of Antitumor Agents from Higher Plants","volume":"38","author":"Agarwal","year":"2022","journal-title":"Biotechnol. Adv."},{"key":"ref_15","first-page":"53","article-title":"Morphological and molecular differentiation between Egyptian species of Pancratium L. (Amaryllidaceae)","volume":"54","author":"Amer","year":"2012","journal-title":"Acta Biol. Crac. Bot."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1007\/s10534-022-00417-1","article-title":"Catalytic potential of endophytes facilitates synthesis of biometallic zinc oxide nanoparticles for agricultural application","volume":"35","author":"Saqib","year":"2022","journal-title":"Biometals"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Xiang, J., Mlambo, R., Shaw, I., Seid, Y., Shah, H., He, Y., Kpegah, J.K.S.K., Tan, S., Zhou, W., and He, B. (2023). Cryopreservation of bioflavonoid-rich plant sources and bioflavonoid-microcapsules: Emerging technologies for preserving bioactivity and enhancing nutraceutical applications. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1232129"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1186\/s12934-023-02234-8","article-title":"Plants and endophytes interaction: A \u201csecret wedlock\u201d for sustainable biosynthesis of pharmaceutically important secondary metabolites","volume":"22","author":"Kumari","year":"2023","journal-title":"Microb. Cell Fact."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"112116","DOI":"10.1016\/j.phytochem.2019.112116","article-title":"Molecular identification and phytochemical screening of endophytic fungi isolated from Lithospermum officinale L. roots: A new source of shikonin","volume":"168","author":"Mollaei","year":"2019","journal-title":"Phytochemistry"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1111\/lam.13542","article-title":"Introducing Alternaria tenuissima SBUp1, as an endophytic fungus of Ferula assafoetida from Iran, which is a rich source of rosmarinic acid","volume":"73","author":"Parvandi","year":"2021","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.sajb.2023.08.024","article-title":"Isolation and molecular identification of endophytic fungi associated with Ziziphora tenuior L. and their biological potential","volume":"161","author":"Ghaffari","year":"2023","journal-title":"S. Afr. J. Bot."},{"key":"ref_22","first-page":"1","article-title":"Fusarium and allied genera from China: Species diversity and distribution","volume":"48","author":"Wang","year":"2022","journal-title":"Pers. Mol. Phylogeny Evol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"19542","DOI":"10.1038\/s41598-024-70694-y","article-title":"The effect of natural herbicide from Fusarium equiseti crude extract on the aquatic weed water hyacinth (Eichornia crassipes (Mart.) Solms)","volume":"14","author":"Laosinwattana","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"576","DOI":"10.5586\/am.576","article-title":"Fusarium equiseti as one of the main Fusarium species causing wilt and root rot of chickpeas in Morocco","volume":"57","author":"Hazzat","year":"2023","journal-title":"Acta Mycol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"178","DOI":"10.21608\/ejp.2025.431979","article-title":"Biological Control of Marjoram Root-Rot Using Kombucha and Natural Antagonists with First Report of Fusarium equiseti in Egypt","volume":"53","author":"Ahmed","year":"2025","journal-title":"Egypt. J. Phytopathol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"107484","DOI":"10.1016\/j.cropro.2025.107484","article-title":"First report of Fusarium equiseti and Fusarium oxysporum causing brown leaf spot on Chinese cabbage","volume":"202","author":"Gu","year":"2026","journal-title":"Crop Prot."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1080\/19440049.2012.698397","article-title":"Trichothecenes and zearalenone production by Fusarium equiseti and Fusarium semitectum species isolated from Argentinean soybean","volume":"29","author":"Barros","year":"2012","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/s41429-020-00379-x","article-title":"Structure determination and cytotoxic evaluation of metabolites from the entomogenous fungus Fusarium equiseti","volume":"74","author":"Liu","year":"2021","journal-title":"J. Antibiot."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"093","DOI":"10.30574\/msarr.2024.10.2.0009","article-title":"Antioxidant and cytotoxicity evaluation of the Endophytic Fungus, Fusarium equiseti, isolated from the leaves of Ocimum gratissimum","volume":"10","author":"Obidiegwu","year":"2024","journal-title":"Magna Sci. Adv. Res. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Pham, G.N., Josselin, B., Cousseau, A., Baratte, B., Dayras, M., Le Meur, C., Debaets, S., Weill, A., Robert, T., and Burgaud, G. (2024). New Fusarochromanone Derivatives from the Marine Fungus Fusarium equiseti UBOCC-A-117302. Mar. Drugs, 22.","DOI":"10.3390\/md22100444"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.flora.2018.03.011","article-title":"Seed germination of Egyptian Pancratium maritimum under salinity with regard to cytology, antioxidant and reserve mobilization enzymes, and seed anatomy","volume":"242","author":"Mohamed","year":"2018","journal-title":"Flora"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1007\/s11274-010-0492-6","article-title":"Isolation, characterization, and bioactivity of endophytic fungi of Tylophora indica","volume":"27","author":"Kumar","year":"2011","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.3934\/microbiol.2024044","article-title":"Aspergillus-Penicillium co-culture: An investigation of bioagents for controlling Fusarium proliferatum-induced basal rot in onion","volume":"10","author":"Abdelrahem","year":"2024","journal-title":"AIMS Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"101462","DOI":"10.1016\/j.jksus.2021.101462","article-title":"Antimicrobial and cytotoxic potential of an endophytic fungus Alternaria tenuissima AUMC14342 isolated from Artemisia judaica L. growing in Saudi Arabia","volume":"33","author":"Mohamed","year":"2021","journal-title":"J. King Saud Univ. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"431","DOI":"10.3109\/09637486.2011.634786","article-title":"Aqueous garlic extract and its phytochemical profile, special reference to antioxidant status","volume":"63","author":"Suleria","year":"2012","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref_36","first-page":"61","article-title":"Spectrophotometric quantification of total alkaloid, flavonoid and phenolic contents of Smilax zeylanica L. Roots","volume":"17","author":"Sutare","year":"2025","journal-title":"J. Xi\u2019an Univ. Archit. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00204-020-02689-3","article-title":"Antioxidants and antioxidant methods: An updated overview","volume":"94","author":"Gulcin","year":"2020","journal-title":"Arch. Toxicol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1055\/s-2007-971236","article-title":"Brine shrimp: A convenient general bioassay for active plant constituents","volume":"45","author":"Meyer","year":"1982","journal-title":"Planta Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1093\/jnci\/82.13.1107","article-title":"New colorimetric cytotoxicity assay for anticancer-drug screening","volume":"82","author":"Skehan","year":"1990","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.toxlet.2018.04.008","article-title":"Fingolimod interrupts the cross talk between estrogen metabolism and sphingolipid metabolism within prostate cancer cells","volume":"291","author":"Allam","year":"2018","journal-title":"Toxicol. Lett."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Kim, C., Le, D., and Lee, M. (2021). Diterpenoids Isolated from Podocarpus macrophyllus Inhibited the Inflammatory Mediators in LPS-Induced HT-29 and RAW 264.7 Cells. Molecules, 26.","DOI":"10.3390\/molecules26144326"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"106171","DOI":"10.1016\/j.bioorg.2022.106171","article-title":"Design, synthesis, and molecular docking of novel pyrazole-chalcone analogs of lonazolac as 5-LOX, iNOS and tubulin polymerization inhibitors with potential anticancer and anti-inflammatory activities","volume":"129","author":"Ahmed","year":"2022","journal-title":"Bioorg. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5724","DOI":"10.1080\/14786419.2021.2019732","article-title":"Phytochemical and in silico studies for potential constituents from Centaurium spicatum as candidates against the SARS-CoV-2 main protease and RNA-dependent RNA polymerase","volume":"36","author":"Allam","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5067","DOI":"10.3390\/cimb44100344","article-title":"Cytotoxic potential of Alternaria tenuissima AUMC14342 mycoendophyte extract: A study combined with LC-MS\/MS metabolic profiling and molecular docking simulation","volume":"44","author":"Abouelela","year":"2022","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"112745","DOI":"10.1016\/j.phytochem.2021.112745","article-title":"In silico and in vitro studies of isolated constituents from Callistemon citrinus leaves: Anti-microbial potential and inhibition of iNOS activity","volume":"186","author":"Abdelmalek","year":"2021","journal-title":"Phytochemistry"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s42250-024-01130-x","article-title":"LC\u2013ESI\u2013MS\/MS Metabolites Profiling, Biological Activity, and Docking Studies of Dendrocalamus strictus Munro","volume":"8","author":"Ibrahim","year":"2025","journal-title":"Chem. Afr."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Abd El-Sadek, M.E., Shahin, H., Yosri, M., Abouelela, M.E., and El-Hela, A.A. (2026). In vitro regeneration of Kroenleinia grusonii (Hildm.) Lod\u00e9 and characterisation of metabolomic profiling, and its antidiabetic potential via in vitro and computational testing. Nat. Prod. Res., 1\u20139.","DOI":"10.1080\/14786419.2026.2616790"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3372","DOI":"10.1021\/acs.jnatprod.0c00717","article-title":"L-Tryptophan induces a marine-derived Fusarium sp. to produce indole alkaloids with activity against the Zika virus","volume":"83","author":"Guo","year":"2020","journal-title":"J. Nat. Prod."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1039\/p19910000997","article-title":"Metabolic products of Fusarium acuminatum: Acuminatopyrone and chlamydosporol","volume":"20","author":"Grove","year":"1991","journal-title":"J. Chem. Soc. Perkin Trans."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1080\/14786419.2019.1650358","article-title":"New naphthoquinone derivatives from Fusarium napiforme of a mangrove plant","volume":"35","author":"Supratman","year":"2019","journal-title":"Nat. Prod. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"685","DOI":"10.7164\/antibiotics.32.685","article-title":"New antibiotic pigments related to fusarubin from Fusarium solani (Mart.) Sacc. II. Structure elucidations","volume":"32","author":"Gerber","year":"1979","journal-title":"J. Antibiot."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2398","DOI":"10.1080\/14786419.2017.1415897","article-title":"The main phytotoxic metabolite produced by a strain of Fusarium oxysporum inducing grapevine plant declining in Italy","volume":"32","author":"Reveglia","year":"2018","journal-title":"Nat. Prod. Res."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Luo, G., Zheng, L., Wu, Q., Chen, S., Li, J., and Liu, L. (2021). Fusarins G\u2013L with inhibition of NO in RAW264. 7 from marine-derived fungus Fusarium solani 7227. Mar. Drugs, 19.","DOI":"10.3390\/md19060305"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1080\/10286020802031106","article-title":"Pyrrole and indole alkaloids from an endophytic Fusarium incarnatum (HKI00504) isolated from the mangrove plant Aegiceras corniculatum","volume":"10","author":"Li","year":"2008","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/0031-9422(95)00716-4","article-title":"Fusaric and 9, 10-dehydrofusaric acids and their methyl esters from Fusarium nygamai","volume":"41","author":"Capasso","year":"1996","journal-title":"Phytochemistry"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"7540","DOI":"10.1016\/j.tet.2011.07.078","article-title":"Dihydronaphthalenones from endophytic fungus Fusarium sp. BCC14842","volume":"67","author":"Kornsakulkarn","year":"2011","journal-title":"Tetrahedron"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1080\/14786419.2022.2116579","article-title":"Novel phenolic metabolites isolated from plant endophytic fungus Fusarium guttiforme","volume":"38","author":"Yue","year":"2024","journal-title":"Nat. Prod. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/j.phytol.2013.06.008","article-title":"Pyrone derivatives from the soil fungus Fusarium solani PSU-RSPG37","volume":"6","author":"Trisuwan","year":"2013","journal-title":"Phytochem. Lett."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"5526","DOI":"10.1039\/C9OB00534J","article-title":"Fusaresters A\u2013E, new \u03b3-pyrone-containing polyketides from fungus Fusarium sp. Hungcl and structure revision of fusariumin D","volume":"17","author":"Gong","year":"2019","journal-title":"Org. Biomol. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2941","DOI":"10.3762\/bjoc.15.289","article-title":"Two new aromatic polyketides from a sponge-derived Fusarium","volume":"15","author":"Sibero","year":"2019","journal-title":"Beilstein J. Org. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1271\/bbb.60.2081","article-title":"Fungerin, a new antifungal alkaloid from Fusarium sp.","volume":"60","author":"Kato","year":"1996","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0031-9422(98)00596-2","article-title":"Jasmonates and related compounds from Fusarium oxysporum","volume":"50","author":"Miersch","year":"1999","journal-title":"Phytochemistry"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"8346","DOI":"10.1021\/jo981226b","article-title":"Neomangicols: Structures and absolute stereochemistries of unprecedented halogenated sesterterpenes from a marine fungus of the genus Fusarium","volume":"63","author":"Renner","year":"1998","journal-title":"J. Org. Chem."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1007\/s10600-017-2092-2","article-title":"A New Nonadride Derivative from the Co-Culture Broth of Two Mangrove Fungi","volume":"53","author":"Ding","year":"2017","journal-title":"Chem. Nat. Compd."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2898","DOI":"10.1039\/p19720002898","article-title":"Fungal products. Part III. Structure of wortmannin and some hydrolysis products","volume":"1","author":"MacMillan","year":"1972","journal-title":"J. Chem. Soc. Perkin Trans."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"113125","DOI":"10.1016\/j.phytochem.2022.113125","article-title":"Decalintetracids A and B, two pairs of unusual 3-decalinoyltetramic acid derivatives with phytotoxicity from Fusarium equiseti D39","volume":"197","author":"Zhao","year":"2022","journal-title":"Phytochemistry"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1111\/j.1462-2920.2011.02696.x","article-title":"Production of novel fusarielins by ectopic activation of the polyketide synthase 9 cluster in Fusarium graminearum","volume":"14","author":"Hansen","year":"2012","journal-title":"Environ. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.phytol.2022.03.014","article-title":"Pyranones from kiwi-associated fungus Fusarium tricinctum and their antibacterial activity","volume":"49","author":"Ma","year":"2022","journal-title":"Phytochem. Lett."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1016\/j.bmc.2017.12.049","article-title":"Fusarithioamide B, a new benzamide derivative from the endophytic fungus Fusarium chlamydosporium with potent cytotoxic and antimicrobial activities","volume":"26","author":"Ibrahim","year":"2018","journal-title":"Bioorg. Med. Chem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1080\/10286020.2014.904853","article-title":"New chlamydosporol derivatives from the endophytic fungus Fusarium sp. #001","volume":"16","author":"Chen","year":"2014","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/j.cclet.2008.06.025","article-title":"5\u2032-Hydroxyzearalenol, a new \u03b2-resorcylic macrolide from Fusarium sp. 05ABR26","volume":"19","author":"Zhao","year":"2008","journal-title":"Chin. Chem. Lett."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"8223","DOI":"10.1021\/ja00203a025","article-title":"Characterization of the Fusarium toxin equisetin: The use of phenylboronates in structure assignment","volume":"111","author":"Phillips","year":"1989","journal-title":"J. Am. Chem. Soc."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1021\/np50115a018","article-title":"Isolation and structure identification of two new derivatives of the mycotoxin Fusaro-chromenone produced by Fusarium equiseti","volume":"58","author":"Xie","year":"1995","journal-title":"J. Nat. Prod."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1021\/np5008137","article-title":"Isolation and structure elucidation of fujikurins A\u2013D: Products of the PKS19 gene cluster in Fusarium fujikuroi","volume":"78","author":"Niehaus","year":"2015","journal-title":"J. Nat. Prod."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1002\/cbic.201600691","article-title":"A fungal N-dimethylallyltryptophan metabolite from Fusarium fujikuroi","volume":"18","author":"Arndt","year":"2017","journal-title":"ChemBioChem"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1080\/14786419.2020.1855165","article-title":"Fusaroxazin, a novel cytotoxic and antimicrobial xanthone derivative from Fusarium oxysporum","volume":"36","author":"Mohamed","year":"2022","journal-title":"Nat. Prod. Res."},{"key":"ref_77","first-page":"01","article-title":"Endophytic Fungi, Fusarium equiseti Produce Amyrin with Cytotoxic Effect","volume":"21","author":"Pillai","year":"2025","journal-title":"EC Microbiol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"100116","DOI":"10.1016\/j.simyco.2021.100116","article-title":"Fusarium: More than a node or a foot-shaped basal cell","volume":"98","author":"Crous","year":"2021","journal-title":"Stud. Mycol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"109297","DOI":"10.1016\/j.ijfoodmicro.2021.109297","article-title":"Phylogenetic analysis and growth profiles of Fusarium incarnatum-equiseti species complex strains isolated from Tunisian cereals","volume":"353","author":"Jedidi","year":"2021","journal-title":"Int. J. Food Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"24935","DOI":"10.1039\/D2RA02532A","article-title":"Anti-diabetic activities of phenolic compounds of Alternaria sp., an endophyte isolated from the leaves of desert plants growing in Egypt","volume":"12","author":"Elbermawi","year":"2022","journal-title":"RSC Adv."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"El-Metwally, M.M., Eisa, A.M., Mekawey, A.A., Mahmoud, S.F., and El Halmouch, Y. (2021). Fungal endophytes of some medicinal plant growing in northwestern coast of Egypt: Isolation, identification and two-way clustering analysis of its antimicrobial activity. Res. Sq.","DOI":"10.21203\/rs.3.rs-828514\/v1"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Hassane, A.M.A., Hussien, S.M., Abouelela, M.E., Taha, T.M., Awad, M.F., Mohamed, H., Hassan, M.M., Hassan, M.H.A., Abo-Dahab, N.F., and El-Shanawany, A.A. (2022). In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.930161"},{"key":"ref_83","first-page":"255","article-title":"Antimicrobial efficacy mediated by mycogenic and characterized selenium nanoparticles","volume":"9","author":"Khalaf","year":"2024","journal-title":"Sohag J. Sci."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1007\/s12668-025-01845-2","article-title":"Facile Mycosynthesis of Selenium Nanoparticles by Aspergillus candidus Extract: Unveiling Characterization, Potent Antimicrobial Properties, and Cytotoxic Activities","volume":"15","author":"Alsehli","year":"2025","journal-title":"BioNanoScience"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ejbt.2022.09.003","article-title":"Biogenesis of nanoparticles with inhibitory effects on aflatoxin B1 production by Aspergillus flavus","volume":"60","author":"Sheikh","year":"2022","journal-title":"Electron. J. Biotechnol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"113793","DOI":"10.1016\/j.inoche.2024.113793","article-title":"Sustainable Myco-Synthesis of antimony oxide nanoparticles using endophytic Penicillium chrysogenum Extract: Characterization, antimicrobial Potency, and cytotoxicity assays","volume":"173","author":"Alsehli","year":"2025","journal-title":"Inorg. Chem. Commun."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s40529-015-0086-6","article-title":"The endophytic fungi of Salvia miltiorrhiza Bge.f. alba are a potential source of natural antioxidants","volume":"56","author":"Li","year":"2015","journal-title":"Bot. Stud."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"332","DOI":"10.5943\/sif\/5\/1\/15","article-title":"Chemical compositions, cytotoxicity and antioxidant activity of the endophytic fungus Fusarium napiforme isolated from Psidium guajava","volume":"5","author":"Chutulo","year":"2020","journal-title":"Stud. Fungi"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"146","DOI":"10.2174\/157340911795677602","article-title":"Molecular docking: A powerful approach for structure-based drug discovery","volume":"7","author":"Meng","year":"2011","journal-title":"Curr. Comput. Aided Drug Des."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s43088-022-00214-2","article-title":"Molecular docking prediction and in vitro studies elucidate anti-inflammatory effect of Garcinia extract against inducible nitric oxide synthase and cyclooxygenase-2 targets","volume":"11","author":"Kalita","year":"2022","journal-title":"Beni-Suef Univ. J. Basic Appl. Sci."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1146\/annurev.immunol.15.1.323","article-title":"Nitric oxide and macrophage function","volume":"15","author":"MacMicking","year":"1997","journal-title":"Annu. Rev. Immunol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/2193-1801-1-69","article-title":"Molecular docking studies of quercetin and its analogues against human inducible nitric oxide synthase","volume":"1","author":"Singh","year":"2012","journal-title":"SpringerPlus"},{"key":"ref_93","first-page":"19","article-title":"Egyptian Pancratium maritimum L. flowers as a source of anti-Alzheimer\u2019s agents","volume":"53","author":"Soltan","year":"2015","journal-title":"Bull. Fac. Pharm. Cairo Univ."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1254\/jphs.11R01CR","article-title":"Pharmacological aspects of the acetylcholinesterase inhibitor galantamine","volume":"116","author":"Ago","year":"2011","journal-title":"J. Pharmacol. Sci."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"61","DOI":"10.4103\/2229-5119.110363","article-title":"Screening of Pancratium maritimum L. for acetylcholinesterase inhibitory alkaloids using thin layer chromatography in combination with bioactivity staining","volume":"4","author":"Shawky","year":"2013","journal-title":"J. Nat. Pharm."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"82","DOI":"10.2174\/1573412911666150611184740","article-title":"Treatment of Alzheimer\u2019s disease: Classical therapeutic approach","volume":"12","author":"Bajic","year":"2016","journal-title":"Curr. Pharm. Anal."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1016\/j.bmc.2014.01.002","article-title":"Ionic liquid mediated synthesis of mono- and bis-spirooxindole-hexahydropyrrolidines as cholinesterase inhibitors and their molecular docking studies","volume":"22","author":"Osman","year":"2014","journal-title":"Bioorg. Med. Chem."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1773\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T04:22:08Z","timestamp":1786681328000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,12]]},"references-count":97,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["microorganisms14081773"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14081773","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,12]]}}}