{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:58:58Z","timestamp":1786978738036,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Copan Italia S.p.A, Brescia, Italy","award":["MAF-25UBM003"],"award-info":[{"award-number":["MAF-25UBM003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Pre-analytical variables, including specimen transport media, may influence the performance of molecular assays used for respiratory pathogen detection. Universal Biomolecular Medium (UBM\u2122) has been developed to support nucleic acid preservation and molecular testing, but evidence regarding its analytical compatibility with routinely used molecular platforms remains limited. We conducted a retrospective paired comparison of UBM\u2122 against conventional transport and lysis media across multiple molecular platforms to evaluate the post-collection analytical compatibility of UBM. Residual respiratory specimens positive for SARS-CoV-2, influenza A virus, influenza B virus, respiratory syncytial virus, human rhinovirus, human metapneumovirus, Haemophilus influenzae, Mycoplasma pneumoniae, and Bordetella pertussis were analyzed. Paired aliquots generated from the same specimen were tested using the Allplex\u2122 SARS-CoV-2\/FluA\/FluB\/RSV Assay, Xpert\u00ae Xpress CoV-2\/Flu\/RSV plus, Panther Fusion\u00ae SARS-CoV-2\/Flu A\/B\/RSV Assay, and selected Allplex\u2122 respiratory panels. Analytical agreement and cycle threshold (Ct) values were compared using paired statistical analyses and Bland\u2013Altman methods. UBM demonstrated complete qualitative concordance with comparator media across all evaluated targets and platforms, yielding 100% positive, negative and overall agreement. Although statistically significant Ct differences were observed for selected target\u2013platform combinations, these shifts were generally small and did not affect qualitative result interpretation. Internal control performance remained stable across media. These findings support the post-collection analytical compatibility of UBM\u2122 with the evaluated molecular workflows.<\/jats:p>","DOI":"10.3390\/microorganisms14081805","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T08:05:41Z","timestamp":1786953941000},"page":"1805","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Universal Biomolecular Medium for Molecular Detection of Respiratory Viruses: A Paired, Multi-Platform Comparison"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1558-032X","authenticated-orcid":false,"given":"Martina","family":"Brandolini","sequence":"first","affiliation":[{"name":"Unit of Microbiology, The Greater Romagna Area Hub Laboratory, 47522 Cesena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3654-7946","authenticated-orcid":false,"given":"Massimiliano","family":"Guerra","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, The Greater Romagna Area Hub Laboratory, 47522 Cesena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7554-9653","authenticated-orcid":false,"given":"Giorgio","family":"Dirani","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, The Greater Romagna Area Hub Laboratory, 47522 Cesena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6296-505X","authenticated-orcid":false,"given":"Silvia","family":"Zannoli","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, The Greater Romagna Area Hub Laboratory, 47522 Cesena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8662-0782","authenticated-orcid":false,"given":"Alessandra Mistral","family":"De Pascali","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences (DIMEC), University of Bologna, 40138 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2297-3009","authenticated-orcid":false,"given":"Laura","family":"Grumiro","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, The Greater Romagna Area Hub Laboratory, 47522 Cesena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Dionisi","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences (DIMEC), University of Bologna, 40138 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4771-8528","authenticated-orcid":false,"given":"Ludovica","family":"Ingletto","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences (DIMEC), University of Bologna, 40138 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1907-8791","authenticated-orcid":false,"given":"Giulia","family":"Gatti","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, The Greater Romagna Area Hub Laboratory, 47522 Cesena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4135-9402","authenticated-orcid":false,"given":"Claudia","family":"Colosimo","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences (DIMEC), University of Bologna, 40138 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1146-0101","authenticated-orcid":false,"given":"Alessandra","family":"Scagliarini","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences (DIMEC), University of Bologna, 40138 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2815-3119","authenticated-orcid":false,"given":"Monica","family":"Cricca","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, The Greater Romagna Area Hub Laboratory, 47522 Cesena, Italy"},{"name":"Department of Medical and Surgical Sciences (DIMEC), University of Bologna, 40138 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5012-7355","authenticated-orcid":false,"given":"Vittorio","family":"Sambri","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, The Greater Romagna Area Hub Laboratory, 47522 Cesena, Italy"},{"name":"Department of Medical and Surgical Sciences (DIMEC), University of Bologna, 40138 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s11908-011-0168-x","article-title":"Molecular diagnosis of viral respiratory infections","volume":"13","author":"Zhang","year":"2011","journal-title":"Curr. 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