{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T08:23:51Z","timestamp":1787559831133,"version":"build-2736575974"},"reference-count":55,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Autonomous University of Mexico","award":["PAPIIT IN222913"],"award-info":[{"award-number":["PAPIIT IN222913"]}]},{"name":"National Autonomous University of Mexico","award":["IG202013"],"award-info":[{"award-number":["IG202013"]}]},{"name":"National Autonomous University of Mexico","award":["IN216316"],"award-info":[{"award-number":["IN216316"]}]},{"name":"National Autonomous University of Mexico","award":["IN202320"],"award-info":[{"award-number":["IN202320"]}]},{"award":["PAPIIT IN222913"],"award-info":[{"award-number":["PAPIIT IN222913"]}],"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}]},{"award":["IG202013"],"award-info":[{"award-number":["IG202013"]}],"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}]},{"award":["IN216316"],"award-info":[{"award-number":["IN216316"]}],"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}]},{"award":["IN202320"],"award-info":[{"award-number":["IN202320"]}],"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Council of Humanities Science and Technology"},{"name":"Secretariat of Science, Humanities, Technology, and Innovation","award":["320415"],"award-info":[{"award-number":["320415"]}]},{"award":["320415"],"award-info":[{"award-number":["320415"]}],"id":[{"id":"https:\/\/ror.org\/059ex5q34","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The bacterial Quorum Sensing system refers to the recognition of signaling molecules called autoinducers produced by bacteria when a certain cell density is reached in the environment. Those cell-density-dependent autoinducers regulate and coordinate diverse functional processes, such as bioluminescence, biofilm production, sporulation, and even the expression of some virulence factors, among others. There is a wide variety of autoinducers, and for Gram-negative bacteria, the canonical autoinducers are the N-acyl-homoserine lactones (AI-1). Presently, the production of autoinducers in Leptospira has not been described; therefore, the objective of this study was to detect and identify autoinducers in this bacterial genus. We report here the expression of AI-1 in cultures \u22652.4 \u00d7 108 of Leptospira meyeri. Ethyl acetate extracts of Leptospira culture supernatants were capable of activating the \u03b2-galactosidase system in the biosensor Agrobacterium tumefaciens strain NTL4. Partial identification of the leptospiral supernatant extracts was done by thin-layer chromatography (TLC), showing a similar retention factor to the synthetic standard N-Octanoyl-DL-homoserine lactone (C8-AHL) in the Leptospira supernatant extracts. In addition, infrared spectroscopy (IR) analysis showed peaks corresponding to the lactone and amide groups in both the C8-AHL standard and the Leptospira meyeri culture extracts. Moreover, High-Performance Liquid Chromatography\u2013Mass Spectrometry (HPLC-MS\/MS) confirmed the same retention time (10.7 \u00b1 0.1 min) in both the Leptospira meyeri supernatant extracts and the C8-AHL standard. These results show that Leptospira meyeri synthesizes N-acyl homoserine lactone family autoinducers, particularly the N-Octanoyl-DL-homoserine lactone, and lay the groundwork for future research on Quorum Sensing systems in Leptospira.<\/jats:p>","DOI":"10.3390\/microorganisms14081806","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T08:05:41Z","timestamp":1786953941000},"page":"1806","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Quorum Sensing Molecules of N-Acyl-Homoserine Lactone in Leptospira Strains Supernatants"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1758-1959","authenticated-orcid":false,"given":"Luz Olivia","family":"Castillo-S\u00e1nchez","sequence":"first","affiliation":[{"name":"<i>Leptospira<\/i> and Leptospirosis Research Group, Teaching, Research and Extension Center for Animal Production in the Plateau, School of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Tequisquiapan 76795, Queretaro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9137-2333","authenticated-orcid":false,"given":"Alejandro","family":"de la Pe\u00f1a-Moctezuma","sequence":"additional","affiliation":[{"name":"<i>Leptospira<\/i> and Leptospirosis Research Group, Teaching, Research and Extension Center for Animal Production in the Plateau, School of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Tequisquiapan 76795, Queretaro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardo Uriel","family":"Bautista-Trujillo","sequence":"additional","affiliation":[{"name":"Chiapas Medicinal Plants Research Institute, Meritorious Autonomous University of Chiapas, Tuxtla Guti\u00e9rrez 29050, Chiapas, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3048-6702","authenticated-orcid":false,"given":"Everardo","family":"Tapia-Mendoza","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, National Autonomous University of Mexico, Coyocan, Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3985-0101","authenticated-orcid":false,"given":"Adriana","family":"Romo-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, National Autonomous University of Mexico, Coyocan, Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Mart\u00ednez-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Biology, Academic Unit for Veterinary Medicine and Zootechnics, Autonomous University of Nayarit, Compostela 63702, Nayarit, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7766-6682","authenticated-orcid":false,"given":"Fidel","family":"Avila-Ramos","sequence":"additional","affiliation":[{"name":"Veterinary and Zootechnics Department, Division of Life Sciences, University of Guanajuato, Irapuato 36500, Guanajuato, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Alfredo","family":"Carmona-Gasca","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Biology, Academic Unit for Veterinary Medicine and Zootechnics, Autonomous University of Nayarit, Compostela 63702, Nayarit, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Vincent, A.T., Schiettekatte, O., Goarant, C., Neela, V.K., Bernet, E., Thibeaux, R., Ismail, N., Mohd-Khalid, M.K.N., Amran, F., and Masuzawa, T. 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