{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T05:23:53Z","timestamp":1787117033534,"version":"build-2736575974"},"reference-count":58,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["268373-E26-211534-2021"],"award-info":[{"award-number":["268373-E26-211534-2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"award":["268373-E26-211534-2021"],"award-info":[{"award-number":["268373-E26-211534-2021"]}],"id":[{"id":"https:\/\/ror.org\/03kk0s825","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Dengue remains one of the most important mosquito-borne viral diseases worldwide, and vector control continues to be the cornerstone of disease prevention. Vertical transmission has been proposed as a mechanism contributing to the maintenance of dengue virus (DENV) during interepidemic periods. In this study, field-collected egg pools and second-generation eggs derived from field-collected females from the state of Rio de Janeiro were analyzed by RT-qPCR, Sanger sequencing, plaque assay, and transmission electron microscopy. The second-generation egg samples (E1\u2013E4), corresponding to F2 eggs of the AEDES-RIO strain, were all DENV positive. Sample E1 was positive for DENV-1, whereas samples E2\u2013E4 were positive for DENV-4, confirming transovarial vertical transmission. The detection of DENV-1 and DENV-2 in field-collected egg pools further supports the occurrence of vertical transmission. All PCR-amplified viral fragments were confirmed by Sanger sequencing. Furthermore, homogenized egg samples naturally infected with DENV successfully infected Vero cells, demonstrating that the virus remained infectious. Transmission electron microscopy revealed virus-like particles of approximately 50 nm in diameter within egg tissues, providing ultrastructural evidence that corroborated the molecular and virological findings. Collectively, these findings provide additional evidence supporting vertical transmission of DENV in naturally infected Aedes aegypti populations and highlight the potential of egg-based surveillance as a complementary tool for entomological monitoring. However, further longitudinal studies are needed to establish its epidemiological value.<\/jats:p>","DOI":"10.3390\/microorganisms14081810","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T08:05:41Z","timestamp":1786953941000},"page":"1810","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of Dengue Virus in Aedes aegypti Eggs Supports Vertical Transmission in Natural Settings"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5822-1426","authenticated-orcid":false,"given":"Tiago Souza","family":"Salles","sequence":"first","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8343-2382","authenticated-orcid":false,"given":"Brenda Martins","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7635-636X","authenticated-orcid":false,"given":"Carlucio","family":"Rocha-Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Sinaliza\u00e7\u00e3o Celular Programa de Biologia Molecular e Biotecnologia, Instituto de Bioqu\u00edmica M\u00e9dica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Laborat\u00f3rio de Biologia, Controle e Vigil\u00e2ncia de Insetos Vetores, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5138-1440","authenticated-orcid":false,"given":"Maria Luiza","family":"Maia","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0805-6140","authenticated-orcid":false,"given":"Svetoslav Nanev","family":"Slavov","sequence":"additional","affiliation":[{"name":"Center for Viral Surveillance and Serological Assessment (CeVIVAs), Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6068-9020","authenticated-orcid":false,"given":"Renata Campos","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Departamento de Virologia, Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Rio de Janeiro 21941-970, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1187-557X","authenticated-orcid":false,"given":"Lucio Ayres","family":"Caldas","sequence":"additional","affiliation":[{"name":"Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biof\u00edsica Carlos Chagas Filho, Centro de Pesquisas em Medicina de Precisao, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil"},{"name":"N\u00facleo Multidisciplinar de Pesquisas em Biologia\u2014NUMPEX-BIO, Campus Duque de Caxias Geraldo Cidade, Universidade Federal do Rio de Janeiro, Duque de Caxias, Rio de Janeiro 25245-390, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3720-415X","authenticated-orcid":false,"given":"Monica Ferreira","family":"Moreira","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Chouin-Carneiro, T., Vega-Rua, A., Vazeille, M., and Yebakima, A. 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