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The present study evaluated whether the probiotic blend\u2019s functional properties are preserved after heat treatment and investigated the underlying mechanism of action using preclinical models. The antioxidant, immunomodulatory and intestinal effects of the heat-treated and probiotic blends were assed using in vitro assays and Caenorhabditis elegans (C. elegans) models. The heat-treated blend preserved the antioxidant activity by scavenging free radicals, reducing intracellular reactive oxygen species and enhancing survival in C. elegans under oxidative stress, possibly via sod-3 upregulation in the CF1553 strain. It also enhanced mitochondrial biogenesis and ATP production in C2C12 via AMPK phosphorylation. Both versions attenuated a gut inflammatory response, improved intestinal barrier in Caco-2 cells and C. elegans and exhibited immunomodulatory activity in U937 macrophages at the tested concentrations. These findings support the heat-treated blend as a promising postbiotic supplement to manage oxidative stress and other physiological alterations associated with intense exercise.<\/jats:p>","DOI":"10.3390\/microorganisms14081820","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:08:41Z","timestamp":1787040521000},"page":"1820","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preservation of Antioxidant and Immunomodulatory Properties in a Heat-Treated Probiotic Blend: Insights from Preclinical Models"],"prefix":"10.3390","volume":"14","author":[{"given":"Daniel","family":"Gonz\u00e1lez-Hedstr\u00f6m","sequence":"first","affiliation":[{"name":"Archer Daniels Midland, Nutrition, R&D Health & Wellness, Biopolis S.L. 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Nutrients, 10.","DOI":"10.3390\/nu10030298"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1113\/jphysiol.2011.225003","article-title":"Muscle Protein Synthesis in Response to Nutrition and Exercise","volume":"590","author":"Atherton","year":"2012","journal-title":"J. Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Meng, Q., and Su, C.-H. (2024). The Impact of Physical Exercise on Oxidative and Nitrosative Stress: Balancing the Benefits and Risks. Antioxidants, 13.","DOI":"10.20944\/preprints202404.0770.v1"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"He, F., Li, J., Liu, Z., Chuang, C.C., Yang, W., and Zuo, L. (2016). Redox Mechanism of Reactive Oxygen Species in Exercise. Front. Physiol., 7.","DOI":"10.3389\/fphys.2016.00486"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8416763","DOI":"10.1155\/2017\/8416763","article-title":"Oxidative Stress: Harms and Benefits for Human Health","volume":"2017","author":"Pizzino","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_6","first-page":"8665","article-title":"Antioxidants Prevent Health-Promoting Effects of Physical Exercise in Humans","volume":"106","author":"Ristow","year":"2009","journal-title":"Med. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1007\/BF03347487","article-title":"The Overtraining Syndrome in Athletes: A Stress-Related Disorder","volume":"27","author":"Angeli","year":"2004","journal-title":"J. Endocrinol. Invest."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1097\/00005768-200002000-00011","article-title":"Cytokine Hypothesis of Overtraining: A Physiological Adaptation to Excessive Stress?","volume":"32","author":"Smith","year":"2000","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"R453","DOI":"10.1016\/j.cub.2014.03.034","article-title":"ROS Function in Redox Signaling and Oxidative Stress","volume":"24","author":"Schieber","year":"2014","journal-title":"Curr. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1038\/s41573-019-0019-2","article-title":"AMP-Activated Protein Kinase: The Current Landscape for Drug Development","volume":"18","author":"Steinberg","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/j.1748-1716.2007.01783.x","article-title":"Exercise Training-Induced Improvements in Insulin Action","volume":"192","author":"Hawley","year":"2008","journal-title":"Acta Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nrm3311","article-title":"AMPK: A Nutrient and Energy Sensor That Maintains Energy Homeostasis","volume":"13","author":"Hardie","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"12017","DOI":"10.1073\/pnas.0705070104","article-title":"AMP-Activated Protein Kinase (AMPK) Action in Skeletal Muscle via Direct Phosphorylation of PGC-1\u03b1","volume":"104","author":"Handschin","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1249\/JSR.0000000000000599","article-title":"Gastrointestinal Issues in Athletes","volume":"18","author":"Coleman","year":"2019","journal-title":"Curr. Sports Med. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"de Kivit, S., Tobin, M.C., Forsyth, C.B., Keshavarzian, A., and Landay, A.L. (2014). Regulation of Intestinal Immune Responses through TLR Activation: Implications for Pro- and Prebiotics. Front. Immunol., 5.","DOI":"10.3389\/fimmu.2014.00060"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cyto.2018.12.006","article-title":"Lifelong Exercise Practice and Immunosenescence: Master Athletes Cytokine Response to Acute Exercise","volume":"115","author":"Minuzzi","year":"2019","journal-title":"Cytokine"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Docherty, S., Harley, R., McAuley, J.J., Crowe, L.A.N., Pedret, C., Kirwan, P.D., Siebert, S., and Millar, N.L. (2022). The Effect of Exercise on Cytokines: Implications for Musculoskeletal Health: A Narrative Review. BMC Sports Sci. Med. Rehabil., 14.","DOI":"10.1186\/s13102-022-00397-2"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Shi, X., Hu, L., Nieman, D.C., Li, F., Chen, P., Shi, H., and Shi, Y. (2025). Exercise Workload: A Key Determinant of Immune Health\u2014A Narrative Review. Front. Immunol., 16.","DOI":"10.3389\/fimmu.2025.1617261"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2529910","DOI":"10.1080\/15502783.2025.2529910","article-title":"Nutritional Strategies for Minimizing Gastrointestinal Symptoms during Endurance Exercise: Systematic Review of the Literature","volume":"22","author":"Mlinaric","year":"2025","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1123\/ijsnem.17.4.352","article-title":"The Effect of Probiotics on Respiratory Infections and Gastrointestinal Symptoms during Training in Marathon Runners","volume":"17","author":"Kekkonen","year":"2007","journal-title":"Int. J. Sport Nutr. Exerc. Metab."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1038\/nrgastro.2014.66","article-title":"The International Scientific Association for Probiotics and Prebiotics Consensus Statement on the Scope and Appropriate Use of the Term Probiotic","volume":"11","author":"Hill","year":"2014","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/s12970-019-0329-0","article-title":"International Society of Sports Nutrition Position Stand: Probiotics","volume":"16","author":"Mohr","year":"2019","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1007\/s00284-011-9915-3","article-title":"Effect of a Probiotic Intake on Oxidant and Antioxidant Parameters in Plasma of Athletes during Intense Exercise Training","volume":"62","author":"Martarelli","year":"2011","journal-title":"Curr. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/1550-2783-9-45","article-title":"Probiotic Supplementation Affects Markers of Intestinal Barrier, Oxidation, and Inflammation in Trained Men; a Randomized, Double-Blinded, Placebo-Controlled Trial","volume":"9","author":"Lamprecht","year":"2012","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/s41575-021-00440-6","article-title":"The International Scientific Association of Probiotics and Prebiotics (ISAPP) Consensus Statement on the Definition and Scope of Postbiotics","volume":"18","author":"Salminen","year":"2021","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Piqu\u00e9, N., Berlanga, M., and Mi\u00f1ana-Galbis, D. (2019). Health Benefits of Heat-Killed (Tyndallized) Probiotics: An Overview. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20102534"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"711","DOI":"10.3920\/BM2019.0015","article-title":"Postbiotics: Facts and Open Questions. A Position Paper on the Need for a Consensus Definition","volume":"10","author":"Collado","year":"2019","journal-title":"Benef. Microbes"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"109502","DOI":"10.1016\/j.foodres.2020.109502","article-title":"Postbiotics and Paraprobiotics: From Concepts to Applications","volume":"136","author":"Liceaga","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Kerksick, C.M., Moon, J.M., and J\u00e4ger, R. (2024). It\u2019s Dead! Can Postbiotics Really Help Performance and Recovery? A Systematic Review. Nutrients, 16.","DOI":"10.3390\/nu16050720"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lee, M.C., Ho, C.S., Hsu, Y.J., and Huang, C.C. (2022). Live and Heat-Killed Probiotic Lactobacillus paracasei PS23 Accelerated the Improvement and Recovery of Strength and Damage Biomarkers after Exercise-Induced Muscle Damage. Nutrients, 14.","DOI":"10.3390\/nu14214563"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Macarro, M., \u00c1vila-Gand\u00eda, V., P\u00e9rez-Pi\u00f1ero, S., C\u00e1novas, F., Garc\u00eda-Mu\u00f1oz, A.M., Abell\u00e1n-ruiz, M.S., Victoria-Montesinos, D., Luque-Rubia, A.J., Climent, E., and Genov\u00e9s, S. (2021). Antioxidant Effect of a Probiotic Product on a Model of Oxidative Stress Induced by High-intensity and Duration Physical Exercise. Antioxidants, 10.","DOI":"10.3390\/antiox10020323"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Martorell, P., Alvarez, B., Llopis, S., Navarro, V., Ortiz, P., Gonzalez, N., Balaguer, F., Rojas, A., Chenoll, E., and Ram\u00f3n, D. (2021). Heat-Treated Bifidobacterium longum CECT-7347: A Whole-Cell Postbiotic with Antioxidant, Anti-Inflammatory, and Gut-Barrier Protection Properties. Antioxidants, 10.","DOI":"10.3390\/antiox10040536"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"463","DOI":"10.3920\/BM2016.0158","article-title":"Differences between Live and Heat-Killed Bifidobacteria in the Regulation of Immune Function and the Intestinal Environment","volume":"8","author":"Sugahara","year":"2017","journal-title":"Benef. Microbes"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s12263-011-0218-x","article-title":"The Immunomodulatory Properties of Probiotic Microorganisms beyond Their Viability (Ghost Probiotics: Proposal of Paraprobiotic Concept)","volume":"6","author":"Taverniti","year":"2011","journal-title":"Genes Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Chen, Y.M., Wei, L., Chiu, Y.S., Hsu, Y.J., Tsai, T.Y., Wang, M.F., and Huang, C.C. (2016). Lactobacillus plantarum TWK10 Supplementation Improves Exercise Performance and Increases Muscle Mass in Mice. Nutrients, 8.","DOI":"10.3390\/nu8040205"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.jff.2017.09.029","article-title":"Heat-Killed Bifidobacterium animalis subsp. lactis CECT 8145 Increases Lean Mass and Ameliorates Metabolic Syndrome in Cafeteria-Fed Obese Rats","volume":"38","author":"Caimari","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Castro-Herrera, V.M., Rasmussen, C., Wellejus, A., Miles, E.A., and Calder, P.C. (2020). In Vitro Effects of Live and Heat-Inactivated Bifidobacterium animalis subsp. lactis, Bb-12 and Lactobacillus rhamnosus GG on Caco-2 Cells. Nutrients, 12.","DOI":"10.3390\/nu12061719"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/genetics\/77.1.71","article-title":"The Genetics of Caenorhabditis elegans","volume":"77","author":"Brenner","year":"1974","journal-title":"Genetics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1021\/jf104217g","article-title":"Use of Saccharomyces cerevisiae and Caenorhabditis elegans as Model Organisms To Study the Effect of Cocoa Polyphenols in the Resistance to Oxidative Stress","volume":"59","author":"Martorell","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1177\/2211068214561025","article-title":"TEER Measurement Techniques for In Vitro Barrier Model Systems","volume":"20","author":"Srinivasan","year":"2015","journal-title":"J. Lab. Autom."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Ayuda-Dur\u00e1n, B., Gonz\u00e1lez-Manzano, S., Miranda-Vizuete, A., Due\u00f1as, M., Santos-Buelga, C., and Gonz\u00e1lez-Param\u00e1s, A.M. (2019). Epicatechin Modulates Stress-Resistance in C. elegans via Insulin\/IGF-1 Signaling Pathway. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0199483"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Suzuki, K., Tominaga, T., Ruhee, R.T., and Ma, S. (2020). Characterization and Modulation of Systemic Inflammatory Response to Exhaustive Exercise in Relation to Oxidative Stress. Antioxidants, 9.","DOI":"10.3390\/antiox9050401"},{"key":"ref_43","first-page":"107","article-title":"Probiotics, Immunity and Exercise: A Review","volume":"15","author":"West","year":"2009","journal-title":"Excercise Immunol. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Di Dio, M., Calella, P., Pelullo, C.P., Liguori, F., Di Onofrio, V., Gall\u00e8, F., and Liguori, G. (2023). Effects of Probiotic Supplementation on Sports Performance and Performance-Related Features in Athletes: A Systematic Review. Int. J. Environ. Res. Public Health, 20.","DOI":"10.3390\/ijerph20032226"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Pham, T.T.H., Huang, W.Y., Chen, C.S., Chiu, W.T., and Chuang, H.S. (2021). Effects of Electrotactic Exercise and Antioxidant EUK-134 on Oxidative Stress Relief in Caenorhabditis elegans. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0245474"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.cmet.2021.03.004","article-title":"Placental Superoxide Dismutase 3 Mediates Benefits of Maternal Exercise on Offspring Health","volume":"33","author":"Kusuyama","year":"2021","journal-title":"Cell Metab."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1249\/MSS.0000000000002827","article-title":"Tart Cherry Supplement Enhances Skeletal Muscle Glutathione Peroxidase Expression and Functional Recovery after Muscle Damage","volume":"54","author":"Wangdi","year":"2022","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Grompone, G., Martorell, P., Llopis, S., Gonz\u00e1lez, N., Genov\u00e9s, S., Mulet, A.P., Fern\u00e1ndez-Calero, T., Tiscornia, I., Bollati-Fogol\u00edn, M., and Chambaud, I. (2012). Anti-Inflammatory Lactobacillus rhamnosus CNCM I-3690 Strain Protects against Oxidative Stress and Increases Lifespan in Caenorhabditis elegans. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0052493"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Khokhlova, E., Colom, J., Simon, A., Mazhar, S., Garc\u00eda-Lainez, G., Llopis, S., Gonzalez, N., Enrique-L\u00f3pez, M., \u00c1lvarez, B., and Martorell, P. (2023). Immunomodulatory and Antioxidant Properties of a Novel Potential Probiotic Bacillus clausii CSI08. Microorganisms, 11.","DOI":"10.3390\/microorganisms11020240"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3462","DOI":"10.1021\/acs.jafc.5b05934","article-title":"Probiotic Strain Bifidobacterium animalis subsp. lactis CECT 8145 Reduces Fat Content and Modulates Lipid Metabolism and Antioxidant Response in Caenorhabditis elegans","volume":"64","author":"Martorell","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1007\/s00253-012-4241-7","article-title":"Antioxidant Properties of Potentially Probiotic Bacteria: In Vitro and in Vivo Activities","volume":"97","author":"Amaretti","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Balaguer, F., Barrena, M., Enrique, M., Maicas, M., \u00c1lvarez, B., Tortajada, M., Chenoll, E., Ram\u00f3n, D., and Martorell, P. (2023). Bifidobacterium animalis subsp. lactis BPL1TM and Its Lipoteichoic Acid Modulate Longevity and Improve Age\/Stress-Related Behaviors in Caenorhabditis elegans. Antioxidants, 12.","DOI":"10.3390\/antiox12122107"},{"key":"ref_53","first-page":"111","article-title":"A Compatibility Assay of Ursolic Acid and Foodborne Microbial Exopolysaccharides by Antioxidant Power and Anti-Proliferative Properties in Hepatocarcinoma Cells","volume":"10","author":"Liu","year":"2012","journal-title":"J. Food Agric. Environ."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.ijbiomac.2014.07.026","article-title":"Antioxidant Activity of Exopolysaccharide from Probiotic Strain Enterococcus faecium (BDU7) from Ngari","volume":"70","author":"Abdhul","year":"2014","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1016\/j.nutres.2014.09.006","article-title":"Dietary Supplementation of Milk Fermented with Probiotic Lactobacillus Fermentum Enhances Systemic Immune Response and Antioxidant Capacity in Aging Mice","volume":"34","author":"Sharma","year":"2014","journal-title":"Nutr. Res."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Chang, H.M., Foo, H.L., Loh, T.C., Lim, E.T.C., and Abdul Mutalib, N.E. (2021). Comparative Studies of Inhibitory and Antioxidant Activities, and Organic Acids Compositions of Postbiotics Produced by Probiotic Lactiplantibacillus plantarum Strains Isolated From Malaysian Foods. Front. Vet. Sci., 7.","DOI":"10.3389\/fvets.2020.602280"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"3174","DOI":"10.1007\/s13197-018-3245-4","article-title":"Antioxidant Effects of Live and Heat-Killed Probiotic Lactobacillus plantarum Ln1 Isolated from Kimchi","volume":"55","author":"Jang","year":"2018","journal-title":"J. Food Sci. Technol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1894","DOI":"10.4014\/jmb.1907.07081","article-title":"Probiotic and Antioxidant Properties of Novel Lactobacillus brevis KCCM 12203P Isolated from Kimchi and Evaluation of Immune-Stimulating Activities of Its Heat-Killed Cells in RAW 264.7 Cells","volume":"29","author":"Song","year":"2019","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2481","DOI":"10.1039\/D2FO03761K","article-title":"Probiotic Activity of Ropy Lactiplantibacillus plantarum NA Isolated from Chinese Northeast Sauerkraut and Comparative Evaluation of Its Live and Heat-Killed Cells on Antioxidant Activity and RAW 264.7 Macrophage Stimulation","volume":"14","author":"Xu","year":"2023","journal-title":"Food Funct."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"546915","DOI":"10.1155\/2012\/546915","article-title":"The Redox System in C. Elegans, a Phylogenetic Approach","volume":"2012","author":"Johnston","year":"2012","journal-title":"J. Toxicol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.fct.2018.09.079","article-title":"Natural Activators of Adenosine 5\u2032-Monophosphate (AMP)-Activated Protein Kinase (AMPK) and Their Pharmacological Activities","volume":"122","author":"Vazirian","year":"2018","journal-title":"Food Chem. Toxicol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Toda, K., Yamauchi, Y., Tanaka, A., Kuhara, T., Odamaki, T., Yoshimoto, S., and Xiao, J.Z. (2020). Heat-Killed Bifidobacterium breve B-3 Enhances Muscle Functions: Possible Involvement of Increases in Muscle Mass and Mitochondrial Biogenesis. Nutrients, 12.","DOI":"10.3390\/nu12010219"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"112188","DOI":"10.1016\/j.biopha.2021.112188","article-title":"Bioactive Compounds from Artemisia Dracunculus L. Activate AMPK Signaling in Skeletal Muscle","volume":"143","author":"Vandanmagsar","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.jff.2017.06.020","article-title":"Effects of Resveratrol, Epigallocatechin Gallate, and Epicatechin on Mitochondrial Functions in C2C12 Myotubes","volume":"35","author":"Chang","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"15522","DOI":"10.1021\/acs.jafc.3c02281","article-title":"Effect of Yeast-Derived Peptides on Skeletal Muscle Function and Exercise-Induced Fatigue in C2C12 Myotube Cells and ICR Mice","volume":"71","author":"Cai","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Saponaro, F., Bertolini, A., Baragatti, R., Galfo, L., Chiellini, G., Saba, A., and D\u2019Urso, G. (2024). Myokines and Microbiota: New Perspectives in the Endocrine Muscle\u2013Gut Axis. Nutrients, 16.","DOI":"10.3390\/nu16234032"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/s41580-022-00547-x","article-title":"New Insights into Activation and Function of the AMPK","volume":"24","author":"Steinberg","year":"2023","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1038\/s41586-025-08629-4","article-title":"Macrophages Recycle Phagocytosed Bacteria to Fuel Immunometabolic Responses","volume":"640","author":"Lesbats","year":"2025","journal-title":"Nature"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Bonomini-Gnutzmann, R., Plaza-D\u00edaz, J., Jorquera-Aguilera, C., Rodr\u00edguez-Rodr\u00edguez, A., and Rodr\u00edguez-Rodr\u00edguez, F. (2022). Effect of Intensity and Duration of Exercise on Gut Microbiota in Humans: A Systematic Review. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph19159518"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Clauss, M., G\u00e9rard, P., Mosca, A., and Leclerc, M. (2021). Interplay Between Exercise and Gut Microbiome in the Context of Human Health and Performance. Front. Nutr., 8.","DOI":"10.3389\/fnut.2021.637010"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"G123","DOI":"10.1152\/ajpgi.00316.2016","article-title":"MiR-200b Inhibits TNF-\u03b1-Induced IL-8 Secretion and Tight Junction Disruption of Intestinal Epithelial Cells in Vitro","volume":"312","author":"Shen","year":"2017","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"109737","DOI":"10.1016\/j.cellsig.2020.109737","article-title":"Mitochondria, the Gut Microbiome and ROS","volume":"75","author":"Ballard","year":"2020","journal-title":"Cell Signal."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Kunst, C., Schmid, S., Michalski, M., T\u00fcmen, D., Buttensch\u00f6n, J., M\u00fcller, M., and G\u00fclow, K. (2023). The Influence of Gut Microbiota on Oxidative Stress and the Immune System. Biomedicines, 11.","DOI":"10.3390\/biomedicines11051388"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"9719","DOI":"10.1021\/acs.jafc.8b03340","article-title":"Exopolysaccharides from Lactobacillus plantarum NCU116 Regulate Intestinal Barrier Function via STAT3 Signaling Pathway","volume":"66","author":"Zhou","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Chen, Y., Zhang, M., and Ren, F. (2019). A Role of Exopolysaccharide Produced by Streptococcus Thermophilus in the Intestinal Inflammation and Mucosal Barrier in Caco-2 Monolayer and Dextran Sulphate Sodium-Induced Experimental Murine Colitis. Molecules, 24.","DOI":"10.3390\/molecules24030513"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.ijbiomac.2021.02.186","article-title":"Protective Effect of Exopolysaccharide Fraction from Bacillus subtilis against Dextran Sulfate Sodium-Induced Colitis through Maintenance of Intestinal Barrier and Suppression of Inflammatory Responses","volume":"178","author":"Chung","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3390\/nutraceuticals3010009","article-title":"Anti-Inflammatory Effects, Protection of Gut Barrier Integrity and Stimulation of Phagocytosis of Postbiotic Combination ABB C1","volume":"3","author":"Vetvicka","year":"2023","journal-title":"Nutraceuticals"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"109","DOI":"10.4161\/gmic.2.2.15661","article-title":"Flexible Cytokine Production by Macrophages and t Cells in Response to Probiotic Bacteria: A Possible Mechanism by Which Probiotics Exert Multifunctional Immune Regulatory Activities","volume":"2","author":"Shida","year":"2011","journal-title":"Gut Microbes"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1159\/000496426","article-title":"Beneficial Effects of Probiotic Consumption on the Immune System","volume":"74","author":"Cazorla","year":"2019","journal-title":"Ann. Nutr. Metab."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"638","DOI":"10.4014\/jmb.2201.01015","article-title":"Immunostimulatory Effect of Heat-Killed Probiotics on RAW264.7 Macrophages","volume":"32","author":"Noh","year":"2022","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"697","DOI":"10.3920\/BM2014.0159","article-title":"Evaluation of the Potential Anti-Allergic Effects of Heat-Inactivated Lactobacillus paracasei V0151 in Vitro, Ex Vivo, and in Vivo","volume":"6","author":"Liu","year":"2015","journal-title":"Benef. Microbes"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"147","DOI":"10.12938\/bmfh.2016-006","article-title":"Structural Diversity and Biological Significance of Lipoteichoic Acid in Gram-Positive Bacteria: Focusing on Beneficial Probiotic Lactic Acid Bacteria","volume":"35","author":"Shiraishi","year":"2016","journal-title":"Biosci. Microbiota Food Health"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.4049\/jimmunol.1701135","article-title":"M1 Means Kill; M2 Means Heal","volume":"199","author":"Ley","year":"2017","journal-title":"J. Immunol."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Atri, C., Guerfali, F.Z., and Laouini, D. (2018). Role of Human Macrophage Polarization in Inflammation during Infectious Diseases. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19061801"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1123\/ijsnem.21.1.55","article-title":"Daily Probiotic\u2019s (Lactobacillus casei Shirota) Reduction of Infection Incidence in Athletes","volume":"21","author":"Gleeson","year":"2011","journal-title":"Int. J. Sport Nutr. Exerc. Metab."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Strasser, B., Geiger, D., Schauer, M., Gostner, J., Gatterer, H., Burtscher, M., and Fuchs, D. (2016). Probiotic Supplements Beneficially Affect Tryptophan\u2013Kynurenine Metabolism and Reduce the Incidence of Upper Respiratory Tract Infections in Trained Athletes: A Randomized, Double-Blinded, Placebo-Controlled Trial. Nutrients, 8.","DOI":"10.3390\/nu8110752"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1139\/apnm-2015-0541","article-title":"Lactobacillus helveticus Lafti\u00ae L10 Supplementation Reduces Respiratory Infection Duration in a Cohort of Elite Athletes: A Randomized Double-Blind Placebo-Controlled Trial","volume":"41","author":"Marinkovic","year":"2016","journal-title":"Appl. Physiol. Nutr. Metab."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s12970-018-0244-9","article-title":"Efficacy of Heat-Killed Lactococcus lactis JCM 5805 on Immunity and Fatigue during Consecutive High Intensity Exercise in Male Athletes: A Randomized, Placebo-Controlled, Double-Blinded Trial","volume":"15","author":"Komano","year":"2018","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1007\/s00421-016-3415-x","article-title":"Effects of Lactobacillus casei Shirota Ingestion on Common Cold Infection and Herpes Virus Antibodies in Endurance Athletes: A Placebo-Controlled, Randomized Trial","volume":"116","author":"Gleeson","year":"2016","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1111\/j.1753-4887.2009.00226.x","article-title":"Probiotic Microbes: Do They Need to Be Alive to Be Beneficial?","volume":"67","author":"Kataria","year":"2009","journal-title":"Nutr. Rev."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.tifs.2016.09.011","article-title":"Paraprobiotics: Evidences on Their Ability to Modify Biological Responses, Inactivation Methods and Perspectives on Their Application in Foods","volume":"58","author":"Almada","year":"2016","journal-title":"Trends Food Sci. Technol."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Vinderola, G., Druart, C., Gos\u00e1lbez, L., Salminen, S., Vinot, N., and Lebeer, S. (2023). Postbiotics in the Medical Field under the Perspective of the ISAPP Definition: Scientific, Regulatory, and Marketing Considerations. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1239745"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1820\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:26:03Z","timestamp":1787041563000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1820"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,18]]},"references-count":92,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["microorganisms14081820"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14081820","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,18]]}}}