{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:28:01Z","timestamp":1787128081719,"version":"build-2736575974"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 32560071"],"award-info":[{"award-number":["No. 32560071"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["No. 32560071"],"award-info":[{"award-number":["No. 32560071"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Science and Technology Department of Qinghai Province","award":["No. 2024-ZJ-941"],"award-info":[{"award-number":["No. 2024-ZJ-941"]}]},{"award":["No. 2024-ZJ-941"],"award-info":[{"award-number":["No. 2024-ZJ-941"]}],"id":[{"id":"https:\/\/ror.org\/03vz7b375","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010230","name":"Qinghai University","doi-asserted-by":"publisher","award":["2025-GMKY-56"],"award-info":[{"award-number":["2025-GMKY-56"]}],"id":[{"id":"10.13039\/501100010230","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2025-GMKY-56"],"award-info":[{"award-number":["2025-GMKY-56"]}],"id":[{"id":"https:\/\/ror.org\/05h33bt13","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>As an endemic woody plant resource widely distributed in the arid regions of northwestern China, Hippophae rhamnoides subsp. sinensis Rousi possesses both ecological and medicinal value. Elucidating the effects of climate and soil conditions on the composition, structure, and function of its root-associated bacterial communities is of practical significance for the development and utilization of these indigenous plant resources in this water-limited region. In this study, root samples of H. rhamnoides were collected from 12 sampling sites in the arid region of Northwest China. High-throughput amplicon sequencing was employed to examine bacterial composition, alpha and beta diversity, molecular co-occurrence networks, and PICRUSt-based functional prediction. Mantel tests and redundancy analysis (RDA) were further applied to identify what is associated with shaping bacterial community structure. The main results are summarized as follows: (1) There were significant differences in the number of ASVs among the various sampling sites, with the P8 site having the highest number (435) and the P5 site having the lowest (156). The dominant phyla in the community were Proteobacteria, Actinobacteria, and Cyanobacteria. (2) Both \u03b1 and \u03b2 diversity showed significant differentiation among the 12 sampling sites. The Ace and Chao1 indices of P8 sampling sites were the highest, while P5 sampling sites were the lowest. In terms of community aggregation, P7 and P12 sampling sites showed tighter clustering, whereas P4 and P5 sampling sites showed more scattered bacterial assemblages. (3) Functional prediction suggested that metabolism, environmental information processing, and genetic information processing functional potential may all be dominant across 12 sampling sites. The abundance of environmental information predicted that the processing functional potential of the P9 sampling site was higher than that of the other 11 sampling sites, while the genetic information processing functional potential was low. (4) Cluster analysis grouped the 12 sampling sites into two groups: sampling sites P10, P11, and P12 were grouped into Group 1, while the remaining 9 sampling sites were grouped into Group 2. (5) RDA revealed that altitude was associated with shaping the root endophytic bacterial community structure of H. rhamnoides, followed by soil total nitrogen. Taken together, the H. rhamnoides populations investigated and sampled in this study were predominantly distributed in neutral-to-alkaline arid soils. The relevant environmental factors are significantly correlated with the alpha diversity patterns of root endophytic bacteria of this species, and they can modulate the community assembly processes, functional allocation characteristics, and co-occurrence network structures of these root endophytic bacteria.<\/jats:p>","DOI":"10.3390\/microorganisms14081829","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T07:58:23Z","timestamp":1787126303000},"page":"1829","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Geographic Variation Characteristics of Endophytic Bacterial Communities in Roots of Hippophae rhamnoides subsp. sinensis Rousi in the Arid Region of Northwest China"],"prefix":"10.3390","volume":"14","author":[{"given":"Hongyuan","family":"San","sequence":"first","affiliation":[{"name":"College of Forestry and Grass, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7132-6788","authenticated-orcid":false,"given":"Pei","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Forestry and Grass, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Forestry and Grass, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guisheng","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Forestry and Grass, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Forestry and Grass, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruisi","family":"Ni","sequence":"additional","affiliation":[{"name":"College of Forestry and Grass, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Forestry and Grass, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Forestry and Grass, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"ref_1","unstructured":"Wang, L.Y. (2025). Diversity and Function of Rhizosphere and Endophytic Microflora in Catalpa bungei. [Master\u2019s Thesis, Ludong University]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s13205-024-03986-9","article-title":"High-throughput sequencing-based analysis of the composition and diversity of the endophytic bacterial community in the roots of Dipsacus asperoides","volume":"14","author":"Liu","year":"2024","journal-title":"3 Biotech"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Guo, S.Y., Ye, G.S., Liu, W.J., Liu, R.Q., Liu, Z.H., and Ma, Y.H. (2024). Environmental factors drive the biogeographic pattern of Hippophae rhamnoides Root endophytic fungal diversity in the arid regions of Northwest China. J. Fungi, 10.","DOI":"10.3390\/jof10100679"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Gao, P., Ye, G.S., and Ma, Y.H. (2025). The bacterial community characteristics of Hippophae rhamnoides Subsp. Sinensis Rousi in different distribution areas of the Qinghai\u2013Tibet plateau and their responses to habitat factors. Biology, 14.","DOI":"10.3390\/biology14091304"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Lyu, L.Y., Gao, P., Xing, Y.F., Ma, J., Liu, Y., Yang, Z.J., Wang, X., and Shi, J.J. (2025). Bacterial community characteristics of Kengyilia thoroldiana rhizosphere soil in different topographic habitats of the Yellow River source region and their response to vegetation-soil factors. Microorganisms, 13.","DOI":"10.3390\/microorganisms13112438"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1134\/S1064229325604822","article-title":"Ecological and geographical features of the structural and functional status of soil microbial cenosis in old-age spruce stands in eastern fennoscandia","volume":"59","author":"Medvedeva","year":"2026","journal-title":"Eurasian Soil Sci."},{"key":"ref_7","first-page":"e04276","article-title":"Can the Natura 2000 pannonian open sand steppes be reborn? The results of 18 years of restoration work in the unique habitat of Hippophae rhamnoides L","volume":"69","author":"Penksza","year":"2026","journal-title":"Glob. Ecol. Conserv."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"101324","DOI":"10.1016\/j.rhisph.2026.101324","article-title":"Overcoming water limitation in Hippophae rhamnoides: AMF symbiosis optimizes root development and water use efficiency under arid conditions","volume":"38","author":"Wu","year":"2026","journal-title":"Rhizosphere"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Gao, P., Ye, G.S., Guo, S.Y., Ma, Y.H., Zhang, Y.Y., Sun, S.X., Guo, L., San, H.Y., Liu, W.J., and Ren, Q.C. (2025). Environmental factors drive the changes of bacterial structure and functional diversity in rhizosphere soil of Hippophae rhamnoides subsp. sinensis Rousi in arid regions of northwest China. Microorganisms, 13.","DOI":"10.3390\/microorganisms13081860"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"905","DOI":"10.2136\/sssaj1969.03615995003300060031x","article-title":"Bicarbonate accumulation and pH changes at the soybean (Glycine max (L.) Merr.) root-soil interface","volume":"33","author":"Riley","year":"1969","journal-title":"Soil Sci. Soc. Am. J."},{"key":"ref_11","unstructured":"Bao, S.D. (2000). Soil Agrochemical Analysis, China Agricultural Press. [3rd ed.]."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"662","DOI":"10.2134\/agronj14.0135","article-title":"Phosphate source interacts with endophyte strain to influence biomass and root system architecture in tall fescue","volume":"107","author":"Ding","year":"2015","journal-title":"Agron. J."},{"key":"ref_13","first-page":"386","article-title":"Effects of stocking rates on soil bacteria and carbon and nitrogen content of Artemisia frigida at different rhizosphere distances","volume":"32","author":"Wang","year":"2024","journal-title":"Acta Agrestia Sin."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e00009-15","DOI":"10.1128\/mSystems.00009-15","article-title":"Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys","volume":"1","author":"William","year":"2016","journal-title":"MSystems"},{"key":"ref_15","first-page":"1","article-title":"Effect of fluoride pollution on the bacterial community in the water-soil system of the Qingshui River Basin in Ningxia","volume":"38","author":"Zhao","year":"2025","journal-title":"J. Lake Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/2047-217X-1-7","article-title":"The Biological Observation Matrix (BIOM) format or: How I learned to stop worrying and love the ome-ome","volume":"1","author":"McDonald","year":"2012","journal-title":"GigaScience"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"170031","DOI":"10.2134\/ael2017.09.0031","article-title":"Functional predictions of microbial communities in soil as affected by long-term tillage practices","volume":"2","author":"Hariharan","year":"2017","journal-title":"Agric. Environ. Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.cell.2016.02.028","article-title":"Root endophyte colletotrichum tofieldiae confers plant fitness benefits that are phosphate status dependent","volume":"165","author":"Hiruma","year":"2016","journal-title":"Cell"},{"key":"ref_19","first-page":"44","article-title":"Analysis on the community diversity of endophytic bacteria in Gymnadenia conopsea at different locations","volume":"46","author":"Yin","year":"2026","journal-title":"J. Microbiol."},{"key":"ref_20","unstructured":"Dong, Z. (2013). Hippophae rhamnoides Root Exudates Acids and Soil Nutrient. [Master\u2019s Thesis, Beijing Forestry University]."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1574-6941.2011.01106.x","article-title":"Growth of saprotrophic fungi and bacteria in soil","volume":"78","author":"Rousk","year":"2011","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_22","unstructured":"Guo, Z.J. (2007). Soil Nutrient and Microorganism as Well Asenzyme Activity in Hippophae rhamnoides and platycladus orientalis Mixed Forest. [Master\u2019s Thesis, Northwest A&F University]."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"155665","DOI":"10.1016\/j.scitotenv.2022.155665","article-title":"Environmental factors, bacterial interactions and plant traits jointly regulate epiphytic bacterial community composition of two alpine grassland species","volume":"836","author":"Li","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Lyu, L.Y., Gao, P., Cai, Z.C., Li, F.Y., and Shi, J.J. (2025). Topographic habitat drive the change of soil fungal community and vegetation soil characteristics in the rhizosphere of Kengyilia thoroldiana in the Sanjiangyuan Region. J. Fungi, 11.","DOI":"10.3390\/jof11070531"},{"key":"ref_25","first-page":"45","article-title":"Analysis of bacterial community characteristics in the rhizosphere soil of wild tree peony","volume":"47","author":"Guo","year":"2023","journal-title":"J. Nanjing For. Univ. (Nat. Sci. Ed.)"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.catena.2017.05.006","article-title":"The diversity and co-occurrence patterns of diazotrophs in the steppes of Inner Mongolia","volume":"157","author":"Wang","year":"2017","journal-title":"Catena"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"107579","DOI":"10.1016\/j.agee.2021.107579","article-title":"New insight into soil carbon fixation rate: The intensive co-occurrence network of autotrophic bacteria increases the carbon fixation rate in depositional sites","volume":"320","author":"Li","year":"2021","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1007\/s13157-024-01862-z","article-title":"The characteristics of soil microbial community structure, soil microbial respiration and their influencing factors of three vegetation types in alpine wetland ecosystem","volume":"44","author":"Wang","year":"2024","journal-title":"Wetlands"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1111\/1758-2229.13065","article-title":"High-throughput sequencing reveals soil bacterial community structure and their interactions with environmental factors of the grassland fairy ring","volume":"14","author":"Wang","year":"2022","journal-title":"Environ. Microbiol. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Bao, S.C., Cai, Z.C., Li, F.Y., Zhang, H.R., Fu, S.Q., Lv, L.Y., Liu, Q.Q., and Shi, J.J. (2026). Changes in soil nutrients and bacterial communities in perennial grass mixtures in alpine ecological zones after 20 years of establishment. Plants, 15.","DOI":"10.3390\/plants15050754"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/j.jes.2025.04.011","article-title":"Green manure plant and exogenous degrading bacteria synergistically promote atrazine removal by enriching indigenous rhizosphere degraders","volume":"159","author":"Hu","year":"2026","journal-title":"J. Environ. Sci."},{"key":"ref_32","first-page":"287","article-title":"Physicochemical properties and bacterial community characteristics of rhizosphere soil in olive orchards under different soil conditions","volume":"42","author":"Zhang","year":"2026","journal-title":"Biotechnol. Bull."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5111","DOI":"10.1128\/AEM.00335-09","article-title":"Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale","volume":"75","author":"Lauber","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_34","first-page":"468","article-title":"Effects of long-term fertilization on diversities of soil microbial community structure and function in grey desert soil of Xinjiang","volume":"35","author":"Xu","year":"2015","journal-title":"Acta Ecol. Sin."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1002\/saj2.20206","article-title":"Alkalinity gradients in grasslands alter soil bacterial community composition and function","volume":"85","author":"Xiang","year":"2021","journal-title":"Soil Sci. Soc. Am. J."},{"key":"ref_36","first-page":"655","article-title":"Effects of different fertilization and plastic mulching on functional diversity of soil microbial community","volume":"27","author":"Hou","year":"2007","journal-title":"Acta Ecol. Sin."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1890\/06-0219","article-title":"Microbial stress-response physiology and its implications for ecosystem function","volume":"88","author":"Schimel","year":"2007","journal-title":"Ecology"},{"key":"ref_38","first-page":"4323","article-title":"Effects of changes in water status on soil microbes and their response mechanism: A review","volume":"30","author":"Zhu","year":"2019","journal-title":"Chin. J. Appl. Ecol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s00248-016-0748-2","article-title":"Abundance and diversity of bacterial, archaeal, and fungal communities along an altitudinal gradient in alpine forest soils: What are the driving factors?","volume":"72","author":"Siles","year":"2016","journal-title":"Microb. Ecol."},{"key":"ref_40","first-page":"883","article-title":"Changes in soil microbial biomass carbon and nitrogen under typical plant communies along an altitudinal gradient in east side of Helan Mountain","volume":"19","author":"Liu","year":"2010","journal-title":"Ecol. Environ. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"166932","DOI":"10.1016\/j.scitotenv.2023.166932","article-title":"Geographical, climatic, and soil factors control the altitudinal pattern of rhizosphere microbial diversity and its driving effect on root zone soil multifunctionality in mountain ecosystems","volume":"904","author":"Yang","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"10541","DOI":"10.1038\/ncomms10541","article-title":"Microbial diversity drives multifunctionality in terrestrial ecosystems","volume":"7","author":"Maestre","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1038\/nature13855","article-title":"Belowground biodiversity and ecosystem functioning","volume":"515","author":"Bardgett","year":"2014","journal-title":"Nature"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"109688","DOI":"10.1016\/j.catena.2025.109688","article-title":"Elevational decline in soil bacterial diversity and its positive role in driving soil multifunctionality: A global meta-analysis","volume":"262","author":"Luo","year":"2026","journal-title":"Catena"},{"key":"ref_45","first-page":"36","article-title":"Changes of soil microbial communities during litter decomposition in alpine forests at different elevations","volume":"29","author":"Hou","year":"2024","journal-title":"J. China Agric. Univ."},{"key":"ref_46","unstructured":"Hu, J.T. (2025). Research on Plant-Microbial Interactions of Dominant Species Along the Elevational Gradient in Wuyi Mountain. [Ph.D. Thesis, Fujian Agriculture and Forestry University]."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1007\/s11104-024-06641-7","article-title":"Relative importance of altitude shifts with plant and microbial diversity to soil multifunctionality in grasslands of north-western China","volume":"504","author":"Wang","year":"2024","journal-title":"Plant Soil"},{"key":"ref_48","first-page":"59","article-title":"The soil bacterial community and diversities of Deyeuxia angustifolia population along different altitude in changbai mountains, Northeastern China","volume":"24","author":"Li","year":"2020","journal-title":"Int. J. Agric. Biol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1829\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:13:58Z","timestamp":1787127238000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":48,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["microorganisms14081829"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14081829","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}