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They are used in industrial production for the manufacture of enzyme preparations, various organic acids, and secondary metabolites. Transcription factors serve as core regulatory molecules governing vital biological processes in filamentous fungi. This review systematically elaborates on the classification, structural features, and functional diversity of transcription factors in filamentous fungi, with a focus on their regulatory roles in hyphal growth, morphological differentiation, metabolite synthesis, and environmental stress responses. We further summarize the key transcription factors in three representative fungal genera, namely, Aspergillus, Trichoderma, and Penicillium. Additionally, the potential applications of genetically engineering transcription factors to increase the yield of metabolites are discussed. This review provides a comprehensive theoretical framework for the genetic modification and high-value utilization of filamentous fungi.<\/jats:p>","DOI":"10.3390\/microorganisms14091892","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T08:11:15Z","timestamp":1787731875000},"page":"1892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Research Advances on the Functions of Transcriptional Regulators in Filamentous Fungi"],"prefix":"10.3390","volume":"14","author":[{"given":"Jia","family":"Su","sequence":"first","affiliation":[{"name":"Key Laboratory of Industrial Microbiology & Engineering Research Center of Food Biotechnology of Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xugang","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Industrial Microbiology & Engineering Research Center of Food Biotechnology of Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yina","family":"Xia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Industrial Microbiology & Engineering Research Center of Food Biotechnology of Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhao","sequence":"additional","affiliation":[{"name":"COFCO Nutrition and Health Research Institute, Beijing 102209, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3203-8869","authenticated-orcid":false,"given":"Yue","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Industrial Microbiology & Engineering Research Center of Food Biotechnology of Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Depei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Industrial Microbiology & Engineering Research Center of Food Biotechnology of Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China"},{"name":"Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianli","family":"Xue","sequence":"additional","affiliation":[{"name":"Key Laboratory of Industrial Microbiology & Engineering Research Center of Food Biotechnology of Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China"},{"name":"Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e0234723","DOI":"10.1128\/spectrum.02347-23","article-title":"An Effective Strategy for Identifying Autogenous Regulation of Transcription Factors in Filamentous fungi","volume":"11","author":"Qin","year":"2023","journal-title":"Microbiol. 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