{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T12:22:49Z","timestamp":1787746969794,"version":"build-2784847793"},"reference-count":52,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004829","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","award":["2025NSFSC2111"],"award-info":[{"award-number":["2025NSFSC2111"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Microbial degradation of phthalate esters (PAEs) is regarded as a highly promising green remediation strategy for PAE-contamination, but it still remains constrained by the scarcity of efficient microbial resources capable of degrading PAEs completely under complex conditions. This study systematically explored microbial resources resistant to dimethyl phthalate (DMP)\/dibutyl phthalate (DBP) stress along with the putative degradation mechanisms in recycled plastic sewage treatment plants through high-throughput sequencing, DMP\/DBP-degrading bacteria screening, and whole-genome sequencing of representative efficient DBP-degrading strains. High-throughput sequencing result revealed substantial enrichment of PAEs-degrading bacteria in activated sludge exposed to 500 mg L\u22121 DMP\/DBP. Further analysis demonstrated that the recycled plastic sewage treatment plant harbored rich culturable DMP\/DBP-degrading bacteria, comprising 19 genera and 27 species of DMP-degrading bacteria, as well as 11 genera and 20 species of DBP-degrading bacteria. Notably, strain SWLYDMP 33, a potential novel species, along with 11 genera such as Paenirhodobacter, Ciceribacter, and Neorhizobium has been scarcely reported in association with PAEs degradation. Given its affiliation with a dominant genus and its superior broad-spectrum degradation performance, the representative efficient DBP-degrading strain SWLYDBP 51 was selected for whole-genome sequencing. Genome annotation of strain SWLYDBP 51 uncovered a wealth of functional genes implicated in DBP metabolism, and the putative complicated DBP degradation pathways were reconstructed, suggesting that SWLYDBP 51 might possess the genetic capacity for effectively degrading DBP under diverse environmental conditions. Overall, this study provided abundant efficient microbial resources and a theoretical reference for the bioremediation of PAEs contamination.<\/jats:p>","DOI":"10.3390\/microorganisms14091896","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T11:53:42Z","timestamp":1787745222000},"page":"1896","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rich Efficient Short-Chain Phthalate Esters-Degrading Bacteria and Their Degradation Mechanisms in Recycled Plastic Wastewater Treatment Plant"],"prefix":"10.3390","volume":"14","author":[{"given":"Zhilian","family":"Gong","sequence":"first","affiliation":[{"name":"College of Food and Biological Engineering, Xihua University, Chengdu 610039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingshan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Food and Biological Engineering, Xihua University, Chengdu 610039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajie","family":"Li","sequence":"additional","affiliation":[{"name":"Faculty of Environmental Engineering, Southwest Jiaotong University, Chengdu 610059, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food and Biological Engineering, Xihua University, Chengdu 610039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sidan","family":"Gong","sequence":"additional","affiliation":[{"name":"Faculty of Environmental Engineering, Southwest Jiaotong University, Chengdu 610059, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Li","sequence":"additional","affiliation":[{"name":"Faculty of Environmental Engineering, Southwest Jiaotong University, Chengdu 610059, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"104066","DOI":"10.1016\/j.eti.2025.104066","article-title":"Biodegradation of various phthalic acid esters at high concentrations by Gordonia alkanivorans GH-1 and its degradation mechanism","volume":"38","author":"Peng","year":"2025","journal-title":"Environ. Technol. Innov."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"113429","DOI":"10.1016\/j.marpolbul.2022.113429","article-title":"The past, present, and future of plastic pollution","volume":"176","author":"Williams","year":"2022","journal-title":"Mar. Pollut. Bull."},{"key":"ref_3","first-page":"149","article-title":"Mechanistic exploration of typical phthalate esters-induced liver injury based on network toxicology and molecular docking techniques","volume":"21","author":"Shang","year":"2026","journal-title":"Asian J. Ecotoxicol."},{"key":"ref_4","first-page":"2142","article-title":"Current status and prospects of research on microbial degradation of phthalate esters","volume":"15","author":"Qiao","year":"2025","journal-title":"J. Environ. Eng. Technol."},{"key":"ref_5","unstructured":"Fang, Y. (2017). Biodegradation Characteristics of Phthalates Degrading Strain Acinetobacter sp. LMB-5 and Properties Analysis of the Esterase. [Master\u2019s Thesis, East China University of Science and Technology]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.ecoenv.2006.11.012","article-title":"Metabolism and biochemical pathway of n-butyl benzyl phthalate by Pseudomonas fluorescens B-1 isolated from a mangrove sediment","volume":"68","author":"Xu","year":"2007","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/S0006-291X(03)01513-4","article-title":"Metabolism of butyl benzyl phthalate by Gordonia sp. strain MTCC 4818","volume":"309","author":"Chatterjee","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.chemosphere.2007.06.058","article-title":"Complete degradation of butyl benzyl phthalate by a defined bacterial consortium: Role of individual isolates in the assimilation pathway","volume":"70","author":"Chatterjee","year":"2008","journal-title":"Chemosphere"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1016\/j.procbio.2004.08.012","article-title":"Fate of phthalic acid esters during composting of both lagooning and activated sludges","volume":"40","author":"Amir","year":"2005","journal-title":"Process Biochem."},{"key":"ref_10","unstructured":"Zhao, Z.Z. (2023). Pathway and Molecular Mechanism of Phthalic Acid Esters Biodegradation by Microbe. [Ph.D. Thesis, University of Science and Technology Beijing]."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/S0045-6535(03)00035-3","article-title":"Dimethylphthalate hydrolysis by specific microbial esterase","volume":"51","author":"Vega","year":"2003","journal-title":"Chemosphere"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1007\/s00253-017-8687-5","article-title":"Bacteria-mediated phthalic acid esters degradation and related molecular mechanisms","volume":"102","author":"Ren","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e70024","DOI":"10.1111\/1751-7915.70024","article-title":"Engineering bacterial biocatalysts for the degradation of phthalic acid esters","volume":"17","author":"Diaz","year":"2024","journal-title":"Microb. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1007\/s00253-024-13105-6","article-title":"Assimilation of phthalate esters in bacteria","volume":"108","author":"Qiao","year":"2024","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"138248","DOI":"10.1016\/j.jhazmat.2025.138248","article-title":"Recent advances in occurrence, biotreatment, and integrated insights into bacterial degradation of phthalic acid esters in aquatic environments","volume":"492","author":"Xian","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s10532-015-9725-6","article-title":"Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate by newly isolated Camelimonas sp. and enzymatic properties of its hydrolase","volume":"26","author":"Chen","year":"2015","journal-title":"Biodegradation"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.jhazmat.2009.11.022","article-title":"Complete degradation of di-n-octyl phthalate by biochemical cooperation between Gordonia sp. strain JDC-2 and Arthrobacter sp. strain JDC-32 isolated from activated sludge","volume":"176","author":"Wu","year":"2010","journal-title":"J. Hazard. Mater."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Li, C., Liu, C., Li, R., Liu, Y., Xie, J., and Li, B. (2022). Biodegradation of Dibutyl Phthalate by the New Strain Acinetobacter baumannii DP-2. Toxics, 10.","DOI":"10.3390\/toxics10090532"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Yi, Y., Wang, Y., Liu, W., Zhu, J., Gu, M., Jia, Q., Li, X., Mutalifu, M., Jiang, L., and Zhang, W. (2025). Screening, identification, metabolic pathway of di-n-butyl phthalate degrading Priestia megaterium P-7 isolated from long-term film mulched cotton field soil in Xinjiang. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1538746"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Fan, S., Feng, Z., Xu, M., Shi, Z., Zhang, Y., Zhang, P., and Hou, X. (2025). The efficient PAE-degrading performance and complete genome sequencing of Gordonia sp. LUNF6. Processes, 13.","DOI":"10.3390\/pr13030731"},{"key":"ref_21","first-page":"82","article-title":"Study on distribution and migration characteristics of PAEs in the municipal wastewater treatment plants in Chaohu Lake Basin","volume":"45","author":"Gao","year":"2023","journal-title":"China Energy Environ. Prot."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3458","DOI":"10.1038\/s41598-022-07132-4","article-title":"The structure of microbial communities of activated sludge of large-scale wastewater treatment plants in the city of Moscow","volume":"12","author":"Begmatov","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_23","first-page":"406","article-title":"Study on the diversity of phthalic acid esters degrading microorganisms in activated sludge from wastewater treatment plant","volume":"42","author":"Wang","year":"2020","journal-title":"Environ. Pollut. Control"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"162201","DOI":"10.1016\/j.scitotenv.2023.162201","article-title":"The fate of phthalate acid esters in wastewater treatment plants and their impact on receiving waters","volume":"873","author":"Wang","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.2174\/1386207323666200630113850","article-title":"Determination of selected phthalates in some commercial cosmetic products by HPLC-UV","volume":"23","author":"Dural","year":"2020","journal-title":"Comb. Chem. High Throughput Screen."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"20210612","DOI":"10.1098\/rsbl.2021.0612","article-title":"Characterization and diversity of native Azotobacter spp. isolated from semi-arid agroecosystems of eastern Kenya","volume":"18","author":"Wakarera","year":"2022","journal-title":"Biol. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Hyatt, D., Chen, G., LoCascio, P., Land, M., Larimer, F., and Hauser, L. (2010). Prodigal: Prokaryotic gene recognition and translation initiation site identification. BMC Bioinform., 11.","DOI":"10.1186\/1471-2105-11-119"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"W451","DOI":"10.1093\/nar\/gki487","article-title":"GeneMark: Web software for gene finding in prokaryotes, eukaryotes and viruses","volume":"33","author":"Besemer","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.scitotenv.2006.12.033","article-title":"Complete degradation of dimethyl isoplithalate requires the biochemical cooperation between Klebsiella oxytoca Sc and Methylobacterium mesophilicum Sr Isolated from Wetland sediment","volume":"380","author":"Li","year":"2007","journal-title":"Sci. Total Environ."},{"key":"ref_30","first-page":"260","article-title":"Biodegradation of phthalate esters by the ultramicrobacterial consortium under the oligotrophic condition","volume":"15","author":"Sun","year":"2015","journal-title":"J. Saf. Environ."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"122863.","DOI":"10.1016\/j.watres.2024.122863","article-title":"Integration of metagenomic analysis and metabolic modeling reveals microbial interactions in activated sludge systems in response to nanoplastics and plasticizers","volume":"271","author":"Wang","year":"2025","journal-title":"Water Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"132044.","DOI":"10.1016\/j.biortech.2025.132044","article-title":"Response of wastewater treatment performance and bacterial community to original and aged polyvinyl chloride microplastics in sequencing batch reactors","volume":"419","author":"Li","year":"2025","journal-title":"Bioresour. Technol."},{"key":"ref_33","first-page":"934","article-title":"Analysis of functional community structure of activated sludge in municipal wastewater treatment processes in China","volume":"46","author":"Guo","year":"2025","journal-title":"Environ. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"126198.","DOI":"10.1016\/j.envpol.2025.126198","article-title":"From plasticizers to pollutants: The ecological consequences of PAEs in agricultural soils","volume":"373","author":"Zou","year":"2025","journal-title":"Environ. Pollut."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"117700","DOI":"10.1016\/j.jece.2025.117700","article-title":"Insights into pollution characteristics of phthalate esters in soil-crop systems and associated responses of soil microbial communities: The case study of Pengzhou, China","volume":"13","author":"Qi","year":"2025","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_36","first-page":"141","article-title":"Relationship between quantitative structure and biodegradability for phthalic acid ester compounds","volume":"30","author":"Xia","year":"2004","journal-title":"J. Zhejiang Univ. Agric. Life Sci."},{"key":"ref_37","first-page":"125","article-title":"Mutational modification of amidohydrolase RhoII derived from Rhodococcus sp. for degradation of phthalate esters","volume":"42","author":"Huang","year":"2024","journal-title":"J. Food Sci. Technol."},{"key":"ref_38","first-page":"1972","article-title":"Fate of eleven phthalic acid esters in aerobic sewage treatment system","volume":"38","author":"Zhou","year":"2017","journal-title":"Environ. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1099\/ijs.0.059774-0","article-title":"Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes","volume":"64","author":"Kim","year":"2014","journal-title":"Evol. Microbiol."},{"key":"ref_40","first-page":"37","article-title":"A novel Niallia nealsonii bacteria degrading phenol isolated from Oum ghellaz lake shore soil in Oran in Algeria","volume":"13","author":"Maghnia","year":"2023","journal-title":"Ukr. J. Ecol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e0117522","DOI":"10.1128\/mra.01175-22","article-title":"Draft genome sequence and annotation of Priestia aryabhattai strain BD1 isolated from a dye sediment","volume":"12","author":"Nsa","year":"2023","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s13205-021-02655-5","article-title":"Isolation and characterization of a novel hydrocarbonoclastic and biosurfactant producing bacterial strain: Fictibacillus phosphorivorans RP3","volume":"11","author":"Pandey","year":"2021","journal-title":"3 Biotech"},{"key":"ref_43","first-page":"35","article-title":"Methylophilaceae and Hyphomicrobium as target taxonomic groups in monitoring the function of methanol-fed denitrification biofilters in municipal wastewater treatment plants","volume":"44","author":"Rissanen","year":"2017","journal-title":"J. Ind. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Vuilleumier, S., Chistoserdova, L., Lee, M.C., Bringel, F., Lajus, A., Zhou, Y., Gourion, B., Barbe, V., Chang, J., and Cruveiller, S. (2009). Methylobacterium Genome Sequences: A reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0005584"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1134\/S0026261720010051","article-title":"Ancylobacter lacus sp. nov. and Ancylobacter plantiphilus sp. nov., novel aerobic facultative methylotrophic bacteria","volume":"89","author":"Chemodurova","year":"2020","journal-title":"Microbiology"},{"key":"ref_46","first-page":"294","article-title":"Diversity of endophytic bacteria in root nodules of Hippophae rhamnoides in the Maxian Mountains","volume":"39","author":"Zhang","year":"2019","journal-title":"Acta Ecol. Sin."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e70268","DOI":"10.1002\/mbo3.70268","article-title":"Bridging the Cultivation Gap in Plant Microbiomes: A Comparative Evaluation of High-Throughput Cultivation and Molecular Profiling","volume":"15","author":"Quattrone","year":"2026","journal-title":"MicrobiologyOpen"},{"key":"ref_48","first-page":"300","article-title":"Primary research of broad-spectrum and biodegradation pathways of phthalic acid esters by Acinetobater baumannii DP-2","volume":"38","author":"Li","year":"2023","journal-title":"For. Ecol. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1007\/s11274-011-0734-2","article-title":"Isolation and characterization of four di-n-butyl phthalate (DBP)-degrading Gordonia sp. strains and cloning the 3,4-phthalate dioxygenase gene","volume":"27","author":"Wu","year":"2011","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s00374-015-1054-8","article-title":"Biodegradation of di-n-butylphthalate and phthalic acid by a novel Providencia sp. 2D and its stimulation in a compost-amended soil","volume":"52","author":"Zhao","year":"2016","journal-title":"Biol. Fertil. Soils"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jbiotec.2018.05.009","article-title":"Complete genome sequence of Gordonia sp. YC-JH1, a bacterium efficiently degrading a wide range of phthalic acid esters","volume":"279","author":"Fan","year":"2018","journal-title":"J. Biotechnol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"143530","DOI":"10.1016\/j.chemosphere.2024.143530","article-title":"Biodegradation of phthalic acid esters by a novel marine bacterial strain RL-BY03: Characterization, metabolic pathway, bioaugmentation and genome analysis","volume":"366","author":"Ren","year":"2024","journal-title":"Chemosphere"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/1896\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T11:59:38Z","timestamp":1787745578000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/1896"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,26]]},"references-count":52,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["microorganisms14091896"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14091896","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,26]]}}}