{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:17:06Z","timestamp":1787894226875,"version":"build-2784847793"},"reference-count":71,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Central project to guide local technological development","award":["2025YD013"],"award-info":[{"award-number":["2025YD013"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32372973"],"award-info":[{"award-number":["32372973"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32260870"],"award-info":[{"award-number":["32260870"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Scientific and Technological Tackling Plan for Key Fields of the Corps","award":["2025AB083"],"award-info":[{"award-number":["2025AB083"]}]},{"name":"the Scientific and Technological Tackling Plan for Key Fields of the Corps","award":["2024AB035"],"award-info":[{"award-number":["2024AB035"]}]},{"name":"Xinjiang Production and Construction Corps Natural Science Foundation Project","award":["2026DA055"],"award-info":[{"award-number":["2026DA055"]}]},{"name":"the Science and Technology Action Project for Rural Revitalization and Industrial Development in the Autonomous Region","award":["2026NC033"],"award-info":[{"award-number":["2026NC033"]}]},{"name":"Tacheng Prefecture Science and Technology Planning Project","award":["2026BB4014"],"award-info":[{"award-number":["2026BB4014"]}]},{"name":"Tianchi talent Project","award":["CZ004310"],"award-info":[{"award-number":["CZ004310"]}]},{"name":"2024 Shihezi University high-level talent research project","award":["RCZK202460"],"award-info":[{"award-number":["RCZK202460"]}]},{"name":"Animal Disease Control and Prevention Technology Innovation Team Project","award":["2025DB016"],"award-info":[{"award-number":["2025DB016"]}]},{"name":"Huyanghe City Science and Technology Program Project","award":["2024C13"],"award-info":[{"award-number":["2024C13"]}]},{"name":"the earmarked fund","award":["CARS-43"],"award-info":[{"award-number":["CARS-43"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Brucella spp. is a bacterium that can survive under conditions of nutrient starvation and is responsible for foodborne illnesses. The OmpR-type transcriptional regulator is characterized by an N-terminal receiver domain and a C-terminal domain, and it plays a regulatory role in diverse physiological processes, notably nitrogen and carbon metabolism. The genome of Brucella melitensis 16M contains genes for multiple OmpR-family regulators. However, the genetic program associated with nitrogen metabolism remains elusive. Herein, it was demonstrated that the B. melitensis 16M \u0394ftcR mutant, which lacks the OmpR-type regulator FtcR, displayed compromised viability upon recovery in a nitrogen- and carbon-free medium. Chromatin immunoprecipitation and next-generation sequencing were used to characterize the DNA-binding sites of FtcR. Our genome-wide analysis revealed extensive FtcR-binding sites throughout the B. melitensis 16M genome, including the identification of glnB as a novel target. As glnB encodes a P-II nitrogen regulator, this suggests FtcR plays a direct role in modulating nitrogen, carbon, and energy metabolism under prolonged nutrient starvation. In summary, our findings not only advance our understanding of the transcriptional regulation of nitrogen metabolism but also highlight its critical role in brucellosis pathogenesis, thereby paving the way for the development of novel therapeutics.<\/jats:p>","DOI":"10.3390\/microorganisms14091898","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:04:32Z","timestamp":1787756672000},"page":"1898","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ability of Brucella melitensis 16M to Control Nitrogen Process: Identification of a Novel P-II Family Nitrogen Regulator"],"prefix":"10.3390","volume":"14","author":[{"given":"Yidan","family":"Zhang","sequence":"first","affiliation":[{"name":"State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengnan","family":"Song","sequence":"additional","affiliation":[{"name":"State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Sun","sequence":"additional","affiliation":[{"name":"State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"},{"name":"Huyanghe City Haoyuanxin Animal Husbandry Development Co., Ltd., Huyanghe 834034, China"},{"name":"Xinjiang Shawan Tianrun Co., Ltd., Shawan 832100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Zhou","sequence":"additional","affiliation":[{"name":"State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuangfu","family":"Chen","sequence":"additional","affiliation":[{"name":"State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Guo","sequence":"additional","affiliation":[{"name":"State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"},{"name":"Xinjiang Aheqi Agriculture and Animal Husbandry Development Co., Ltd., Aheqi 843500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-1097(03)00963-7","article-title":"Brucella pathogenesis, genes identified from random large-scale screens","volume":"231","author":"Delrue","year":"2004","journal-title":"FEMS Microbiol. 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