{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T05:18:30Z","timestamp":1787980710764,"version":"build-2784847793"},"reference-count":48,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Chagas disease is difficult to diagnose in the chronic phase, especially when serological assays disagree. We examined whether Trypanosoma cruzi metacyclic trypomastigotes\u2019 antigenic extracts may increase ELISA\/Western blot concordance in initially discordant serum compared to epimastigotes. A total of 178 human blood samples were studied, including seropositive persons, Chagasic cardiomyopathy patients, discordant sera, seronegative controls, and sera from various parasitic illnesses. Antigenic extracts from epimastigotes and metacyclic trypomastigotes were extracted using sonication or urea, and Western blot and ELISA were used to compare their behavior. Sonicated metacyclic trypomastigote extracts produced concordant ELISA\/Western blot results in 31\/35 originally discordant sera, compared to 19\/35 for sonicated epimastigotes, 20\/35 for urea-extracted trypomastigotes, and 17\/35 for urea-extracted epimastigotes. All extracts in the serum panel previously classified using the standardized in-house serological platform showed 100% positive percent agreement, but negative percent agreement varied between 93.3% for sonicated metacyclic trypomastigotes and 66.7% for urea-extracted epimastigotes. These estimates reflect agreement with the previous serological classification, not diagnostic accuracy versus an independent reference standard. Cross-reactivity was mainly observed in sera from patients with leishmaniasis, while sera from the cardiopathic group showed an exploratory preferential recognition pattern with bands of 18, 22, 24, and 26 kDa in the sonicated metacyclic trypomastigote preparation. The comparison did not account for potential clinical variables or molecularly identify these bands. These data demonstrate that stage-informed antigen selection, particularly using sonicated metacyclic trypo-mastigote extracts, can affect serum panel assay concordance and nonspecific reactivity.<\/jats:p>","DOI":"10.3390\/microorganisms14091899","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T08:02:27Z","timestamp":1787817747000},"page":"1899","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stage-Informed Trypanosoma cruzi Antigen Selection Enhances Assay Concordance in the Evaluation of Discordant Chagas Disease Serology"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1415-6260","authenticated-orcid":false,"given":"Luis Adri\u00e1n","family":"De Jes\u00fas-Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Unidad de Investigaci\u00f3n Biom\u00e9dica de Zacatecas, Instituto Mexicano del Seguro Social, Zacatecas 98000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1108-3641","authenticated-orcid":false,"given":"Ignacio","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Auton\u00f3ma de Mexico (UNAM), Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Procopio-Vel\u00e1zquez","sequence":"additional","affiliation":[{"name":"Hospital de Infectolog\u00eda, Centro M\u00e9dico Nacional \u201cLa Raza\u201d, Instituto Mexicano del Seguro Social, Mexico City 02990, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muslim","family":"Schabib-Hany","sequence":"additional","affiliation":[{"name":"Hospital de Infectolog\u00eda, Centro M\u00e9dico Nacional \u201cLa Raza\u201d, Instituto Mexicano del Seguro Social, Mexico City 02990, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertha","family":"Espinoza","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Auton\u00f3ma de Mexico (UNAM), Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1186\/1756-3305-7-317","article-title":"The History of Chagas Disease","volume":"7","author":"Steverding","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Espinoza, B., Mart\u00ednez, I., Mart\u00ednez-Velasco, M.L., Rodr\u00edguez-Sosa, M., Gonz\u00e1lez-Canto, A., V\u00e1zquez-Mendoza, A., and Terrazas, L.I. (2023). Role of a 49 kDa Trypanosoma cruzi Mucin-Associated Surface Protein (MASP49) during the Infection Process and Identification of a Mammalian Cell Surface Receptor. Pathogens, 12.","DOI":"10.3390\/pathogens12010105"},{"key":"ref_3","unstructured":"(2026, August 06). Chagas Disease. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/chagas-disease-(american-trypanosomiasis)."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Sereno, D., Oury, B., and Grijalva, M.J. (2024). Chagas Disease across the Ages: A Historical View and Commentary on Navigating Future Challenges. Microorganisms, 12.","DOI":"10.3390\/microorganisms12061153"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"103673","DOI":"10.1016\/j.ibmb.2021.103673","article-title":"Differential Activity on Trypanosomatid Parasites of a Novel Recombinant Defensin Type 1 from the Insect Triatoma (Meccus) pallidipennis","volume":"139","author":"Poggio","year":"2021","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Robertson, L.J., Havelaar, A.H., Keddy, K.H., Devleesschauwer, B., Sripa, B., and Torgerson, P.R. (2024). The Importance of Estimating the Burden of Disease from Foodborne Transmission of Trypanosoma cruzi. PLoS Neglected Trop. Dis., 18.","DOI":"10.1371\/journal.pntd.0011898"},{"key":"ref_7","first-page":"25","article-title":"Diagnosis and Clinical Management of Chagas Disease: An Increasing Challenge in Non-Endemic Areas","volume":"13","author":"Nolder","year":"2022","journal-title":"Res. Rep. Trop. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/s40249-023-01143-7","article-title":"Molecular Diagnosis of Chagas Disease: A Systematic Review and Meta-Analysis","volume":"12","author":"Muro","year":"2023","journal-title":"Infect. Dis. Poverty"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.actatropica.2017.09.017","article-title":"Trypanosoma cruzi Genetic Diversity: Something New for Something Known about Chagas Disease Manifestations, Serodiagnosis and Drug Sensitivity","volume":"184","author":"Zingales","year":"2018","journal-title":"Acta Trop."},{"key":"ref_10","first-page":"100821","article-title":"Retrospect, Advances and Challenges in Chagas Disease Diagnosis: A Comprehensive Review","volume":"36","author":"Schijman","year":"2024","journal-title":"Lancet Reg. Health\u2014Am."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"47","DOI":"10.7705\/biomedica.5441","article-title":"Disagreement between PCR and Serological Diagnosis of Trypanosoma cruzi Infection in Blood Donors from a Colombian Endemic Region","volume":"41","author":"Aguilar","year":"2021","journal-title":"Biomedica"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Marchiol, A., Sanchez, A.C.F., Caicedo, A., Segura, M., Bautista, J., Sotelo, M.S.A., Herazo, R., Forsyth, C., and Bohorquez, L.C. (2023). Laboratory Evaluation of Eleven Rapid Diagnostic Tests for Serological Diagnosis of Chagas Disease in Colombia. PLoS Neglected Trop. Dis., 17.","DOI":"10.1371\/journal.pntd.0011547"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ossowski, M.S., Gallardo, J.P., Niborski, L.L., Rodr\u00edguez-Dur\u00e1n, J., Lapadula, W.J., Juri Ayub, M., Chadi, R., Hernandez, Y., Fernandez, M.L., and Potenza, M. (2024). Characterization of Novel Trypanosoma cruzi-Specific Antigen with Potential Use in the Diagnosis of Chagas Disease. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25021202"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e12786","DOI":"10.1111\/pim.12786","article-title":"Host-Parasite Dynamics in Chagas Disease from Systemic to Hyper-Local Scales","volume":"43","author":"Ward","year":"2021","journal-title":"Parasite Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1128\/JCM.01649-16","article-title":"Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells","volume":"55","author":"Ndao","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"ref_16","first-page":"365403","article-title":"High Molecular Weight Proteins of Trypanosoma cruzi Reduce Cross-Reaction with Leishmania Spp. in Serological Diagnosis Tests","volume":"2014","author":"Schabib","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/1756-3305-1-20","article-title":"Different Serological Cross-Reactivity of Trypanosoma rangeli Forms in Trypanosoma cruzi-Infected Patients Sera","volume":"1","author":"Guarneri","year":"2008","journal-title":"Parasites Vectors"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3642","DOI":"10.1021\/pr800887u","article-title":"Proteomic Analysis of Detergent-Solubilized Membrane Proteins from Insect-Developmental Forms of Trypanosoma cruzi","volume":"8","author":"Cordero","year":"2009","journal-title":"J. Proteome Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/S0188-4409(01)00303-4","article-title":"Standardization of Micro-Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blot for Detection of Trypanosoma cruzi Antibodies Using Extracts from Mexican Strains as Antigens","volume":"32","author":"Reyes","year":"2001","journal-title":"Arch. Med. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3803","DOI":"10.1007\/s00436-020-06891-1","article-title":"Differential Gene Expression of Virulence Factors Modulates Infectivity of TcI Trypanosoma cruzi Strains","volume":"119","author":"Lujan","year":"2020","journal-title":"Parasitol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Schaub, G.A. (2024). Interaction of Trypanosoma cruzi, Triatomines and the Microbiota of the Vectors\u2014A Review. Microorganisms, 12.","DOI":"10.3390\/microorganisms12050855"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"875909","DOI":"10.1155\/2012\/875909","article-title":"Development and Application of an ELISA Assay Using Excretion\/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease","volume":"2012","author":"Berrizbeitia","year":"2012","journal-title":"J. Trop. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/j.1744-7917.2012.01566.x","article-title":"The Potential of Trehalose to Replace Insect Hemolymph in Artificial Media for Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae)","volume":"20","author":"Han","year":"2013","journal-title":"Insect Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ferreira, A.Z.L., de Ara\u00fajo, C.N., Cardoso, I.C.C., de Souza Mangabeira, K.S., Rocha, A.P., Charneau, S., Santana, J.M., Motta, F.N., and Bastos, I.M.D. (2023). Metacyclogenesis as the Starting Point of Chagas Disease. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25010117"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Cestari, I., and Ramirez, M.I. (2010). Inefficient Complement System Clearance of Trypanosoma cruzi Metacyclic Trypomastigotes Enables Resistant Strains to Invade Eukaryotic Cells. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0009721"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1002\/elps.1150180303","article-title":"Improvement of the Solubilization of Proteins in Two-Dimensional Electrophoresis with Immobilized pH Gradients","volume":"18","author":"Rabilloud","year":"1997","journal-title":"Electrophoresis"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1128\/JCM.00142-16","article-title":"Serological Diagnosis of Chronic Chagas Disease: Is It Time for a Change?","volume":"54","author":"Abras","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"ITC17","DOI":"10.7326\/AITC202302210","article-title":"Chagas Disease","volume":"176","author":"Hochberg","year":"2023","journal-title":"Ann. Intern. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e240282","DOI":"10.1590\/0074-02760240282","article-title":"A Multicentre Comparative Study of Serological Methods for Diagnosing Chagas Disease in Brazil","volume":"120","author":"Ostermayer","year":"2025","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Brossas, J.-Y., Griselda, B., Bisio, M., Guihenneuc, J., Gulin, J.E.N., Jaur\u00e9guiberry, S., Lescure, F.-X., Fekkar, A., Mazier, D., and Altcheh, J. (2021). Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease. Pathogens, 10.","DOI":"10.3390\/pathogens10111455"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1086\/597464","article-title":"Spatial Patterns in Discordant Diagnostic Test Results for Chagas Disease: Links to Transmission Hotspots","volume":"48","author":"Levy","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"ref_32","first-page":"499","article-title":"Chagasic cardiomyopathy in Mexico","volume":"56","author":"Reyes","year":"1986","journal-title":"Arch. Cardiol. M\u00e9xico"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1590\/S0074-02762003000500004","article-title":"Electrocardiographic Findings in Mexican Chagasic Subjects Living in High and Low Endemic Regions of Trypanosoma cruzi Infection","volume":"98","author":"Reyes","year":"2003","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_34","first-page":"4","article-title":"Cardiac Abnormalities in Trypanosoma cruzi Seropositive Patients in the State of Quer\u00e9taro, M\u00e9xico","volume":"28","author":"Linares","year":"2017","journal-title":"Rev. Mex. Cardiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1128\/CVI.00127-07","article-title":"Evaluation of Serological Tests to Identify Trypanosoma cruzi Infection in Humans and Determine Cross-Reactivity with Trypanosoma rangeli and Leishmania Spp.","volume":"14","author":"Caballero","year":"2007","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Teixeira-Carvalho, A., Campos, F.M.F., Geiger, S.M., Rocha, R.D.R., de Ara\u00fajo, F.F., Vitelli-Avelar, D.M., Andrade, M.C., Ara\u00fajo, M.S.S., Lemos, E.M., and de Freitas Carneiro Proietti, A.B. (2015). FC-TRIPLEX Chagas\/Leish IgG1: A Multiplexed Flow Cytometry Method for Differential Serological Diagnosis of Chagas Disease and Leishmaniasis. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0122938"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Luquetti, A.O., and Schmu\u00f1is, G.A. (2017). Diagnosis of Trypanosoma cruzi Infection. American Trypanosomiasis Chagas Disease, Elsevier.","DOI":"10.1016\/B978-0-12-801029-7.00030-7"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Jes\u00fas-Gonz\u00e1lez, L.A.D., Mart\u00ednez, I., Espinoza, B., and Lira-Hern\u00e1ndez, F.I. (2025). Is Sexual Transmission of Chagas Disease Possible? Evaluating the Evidence and Future Directions. Pathogens, 14.","DOI":"10.3390\/pathogens14111124"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1128\/jcm.34.9.2143-2147.1996","article-title":"Immunoblot Assay Using Excreted-Secreted Antigens of Trypanosoma cruzi in Serodiagnosis of Congenital, Acute, and Chronic Chagas\u2019 Disease","volume":"34","author":"Umezawa","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1128\/JCM.44.2.291-296.2006","article-title":"Purified Excreted-Secreted Antigens from Trypanosoma cruzi Trypomastigotes as Tools for Diagnosis of Chagas\u2019 Disease","volume":"44","author":"Berrizbeitia","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"412","DOI":"10.4269\/ajtmh.2012.11-0111","article-title":"Identification of a Western Blot Pattern for the Specific Diagnosis of Trypanosoma cruzi Infection in Human Sera","volume":"86","author":"Riera","year":"2012","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2086","DOI":"10.1128\/jcm.33.8.2086-2090.1995","article-title":"Use of a 24-Kilodalton Trypanosoma cruzi Recombinant Protein to Monitor Cure of Human Chagas\u2019 Disease","volume":"33","author":"Krautz","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Versteeg, L., Adhikari, R., Poveda, C., Villar-Mondragon, M.J., Jones, K.M., Hotez, P.J., Bottazzi, M.E., Tijhaar, E., and Pollet, J. (2021). Location and Expression Kinetics of Tc24 in Different Life Stages of Trypanosoma cruzi. PLoS Neglected Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009689"},{"key":"ref_44","first-page":"6","article-title":"Identificaci\u00f3n de componentes inmunodominantes de un aislado de Trypanosoma cruzi por inmunoblot y su estandarizaci\u00f3n con fines diagn\u00f3sticos","volume":"151","year":"2015","journal-title":"Gac. M\u00e9dica M\u00e9xico"},{"key":"ref_45","first-page":"85","article-title":"Seroprevalence and Major Antigens Recognized by Sera from Trypanosoma cruzi-Infected Dogs from Jalisco, M\u00e9xico","volume":"46","author":"Espinoza","year":"2014","journal-title":"Rev. Argent. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Resende, C.A.A., Ribeiro, A.J., Gandra, I.B., Silva, K.A., da Silva Lopes, L., Barcelos, I.C.D.S., Couto, C.A.P., de Faria, M.T., Pereira, S.P., and Xavier, S.R. (2024). Recombinant Proteins as Promising Antigens Applied to the Immunodiagnosis of Chagas Disease: A Scoping Review. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1420226"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"dos Santos, E.F., Silva, \u00c2.A.O., Freitas, N.E.M., Leony, L.M., Daltro, R.T., de Souza Teles Santos, C.A., Almeida, M.d.C.C.d., de Ara\u00fajo, F.L.V., Celedon, P.A.F., and Krieger, M.A. (2022). Performance of Chimeric Trypanosoma cruzi Antigens in Serological Screening for Chagas Disease in Blood Banks. Front. Med., 9.","DOI":"10.3389\/fmed.2022.852864"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Dopico, E., Del-Rei, R.P., Espinoza, B., Ubillos, I., Zanchin, N.I.T., Sulleiro, E., Moure, Z., Celedon, P.A.F., Souza, W.V., and da Silva, E.D. (2019). Immune Reactivity to Trypanosoma cruzi Chimeric Proteins for Chagas Disease Diagnosis in Immigrants Living in a Non-Endemic Setting. BMC Infect. Dis., 19.","DOI":"10.1186\/s12879-019-3872-z"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/1899\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T04:33:55Z","timestamp":1787978035000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/1899"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,27]]},"references-count":48,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["microorganisms14091899"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14091899","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,27]]}}}