{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T09:24:04Z","timestamp":1788341044813,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001348","name":"Agency of Science, Technology and Research","doi-asserted-by":"publisher","award":["C221418005"],"award-info":[{"award-number":["C221418005"]}],"id":[{"id":"10.13039\/501100001348","id-type":"DOI","asserted-by":"publisher"}]},{"award":["C221418005"],"award-info":[{"award-number":["C221418005"]}],"id":[{"id":"https:\/\/ror.org\/036wvzt09","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Surveillance of methicillin-resistant Staphylococcus aureus (MRSA) in the environment is crucial for reducing its transmission, particularly in hospitals, nursing homes, and high-contact public areas. Rapid Point-of-Need (PON) tools at healthcare facilities, without involving prolonged bacterial culture in sophisticated laboratories, are needed for environmental surveillance of MRSA. We have developed a gold nanoparticle (AuNP)-based \u201cSwab-and-Stain\u201d assay for rapid and on-site detection of PBP2a-expressing MRSA from environmental surfaces. The assay utilizes anti-PBP2a antibody immobilized on cotton swabs to specifically capture PBP2a-expressing MRSA and anti-MRSA antibody conjugated on AuNPs to detect the captured MRSA on the swabs via sandwiched complex formation. In the presence of PBP2a-expressing MRSA, the swabs were stained in red, as seen with the naked eye. The color intensity of the stain can be quantified by smartphone image analysis for MRSA quantification. Using PBP2a-expressing MRSA samples spiked in real hospital sink and drain matrices, this pilot study demonstrated a \u201cSwab-and-Stain\u201d assay as a proof-of-concept method for detecting MRSA at a concentration as low as 12 CFU\/mL. The assay was validated to specifically detect MRSA spiked in real hospital sink and drain matrices. This \u201cSwab-and-Stain\u201d assay provides a rapid, portable, and easy-to-use platform with potential application for PON environmental surveillance of MRSA.<\/jats:p>","DOI":"10.3390\/microorganisms14091940","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T08:56:01Z","timestamp":1788339361000},"page":"1940","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["\u201cSwab-and-Stain\u201d Nanoparticle Assay for Detection of PBP2a-Expressing Methicillin-Resistant Staphylococcus aureus Toward Point-of-Need Environmental Surveillance"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4110-2831","authenticated-orcid":false,"given":"Laura","family":"Sutarlie","sequence":"first","affiliation":[{"name":"Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore 138634, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Sian Yang","family":"Ow","sequence":"additional","affiliation":[{"name":"Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore 138634, 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Technology and Research (A*STAR), 60 Biopolis Street, Genome, Singapore 138672, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Kar Mun","family":"Lim","sequence":"additional","affiliation":[{"name":"Genome Institute of Singapore (GIS), Agency for Science, Technology and Research (A*STAR), 60 Biopolis Street, Genome, Singapore 138672, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Patipan","family":"Boonsimma","sequence":"additional","affiliation":[{"name":"Genome Institute of Singapore (GIS), Agency for Science, Technology and Research (A*STAR), 60 Biopolis Street, Genome, Singapore 138672, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Darren Wei","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Singapore General Hospital, 20 College Road, Singapore 169856, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Niranjan","family":"Nagarajan","sequence":"additional","affiliation":[{"name":"Genome Institute of Singapore (GIS), Agency for Science, Technology and Research (A*STAR), 60 Biopolis Street, Genome, Singapore 138672, Singapore"},{"name":"Department of Biochemistry, Yong Loo Lin School of Medicine, National University of 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Mortal. Wkly. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Nikolic, P., and Mudgil, P. (2023). The Cell Wall, Cell Membrane and Virulence Factors of Staphylococcus aureus and Their Role in Antibiotic Resistance. Microorganisms, 11.","DOI":"10.3390\/microorganisms11020259"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1080\/21505594.2016.1270494","article-title":"Antibodies to Staphylococcus aureus Capsular Polysaccharides 5 and 8 Perform Similarly in vitro but are Functionally Distinct in vivo","volume":"8","author":"Liu","year":"2017","journal-title":"Virulence"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.diagmicrobio.2007.01.016","article-title":"Distribution of Capsular and Surface Polysaccharide Serotypes of Staphylococcus aureus","volume":"58","author":"Taylor","year":"2007","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/s41579-018-0147-4","article-title":"Methicillin-Resistant Staphylococcus aureus: An Overview of Basic and Clinical Research","volume":"17","author":"Turner","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Li, J., Cheng, F., Wei, X., Bai, Y., Wang, Q., Li, B., Zhou, Y., Zhai, B., Zhou, X., and Wang, W. (2025). Methicillin-Resistant Staphylococcus aureus (MRSA): Resistance, Prevalence, and Coping Strategies. Antibiotics, 14.","DOI":"10.3390\/antibiotics14080771"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"117048","DOI":"10.1016\/j.bcp.2025.117048","article-title":"Targeting MRSA Penicillin-Binding Protein 2a: Structural Insights, Allosteric Mechanisms, and the Potential of Adjuvant Inhibitors","volume":"239","author":"Rosado","year":"2025","journal-title":"Biochem. Pharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1146\/annurev-biochem-060614-034516","article-title":"Mechanisms of Methicillin Resistance in Staphylococcus aureus","volume":"84","author":"Peacock","year":"2015","journal-title":"Annu. Rev. Biochem."},{"key":"ref_9","unstructured":"World Health Organization (2024). WHO Bacterial Priority Pathogens List, 2024: Bacterial Pathogens of Public Health Importance to Guide Research, Development and Strategies to Prevent and Control Antimicrobial Resistance, World Health Organization. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789240093461."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1001\/jama.298.15.1763","article-title":"Invasive Methicillin-Resistant Staphylococcus aureus Infections in the United States","volume":"298","author":"Klevens","year":"2007","journal-title":"JAMA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e00437-23","DOI":"10.1128\/aac.00437-23","article-title":"Failure of mecA\/mecC PCR Testing to Accurately Predict Oxacillin Resistance in a Patient with Staphylococcus aureus Infective Endocarditis","volume":"67","author":"Hess","year":"2023","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e0288923","DOI":"10.1128\/mbio.02889-23","article-title":"Altered PBP4 and GdpP functions synergistically mediate MRSA-like high-level, broad-spectrum \u03b2-lactam resistance in Staphylococcus aureus","volume":"15","author":"Lai","year":"2024","journal-title":"mBio"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1086\/660363","article-title":"The Role Played by Contaminated Surfaces in the Transmission of Nosocomial Pathogens","volume":"32","author":"Otter","year":"2011","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1093\/cid\/cix901","article-title":"Frequent Undetected Ward-Based Methicillin-Resistant Staphylococcus aureus Transmission Linked to Patient Sharing Between Hospitals","volume":"66","author":"Stabler","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.jhin.2021.02.021","article-title":"How Dirty is Your QWERTY? The Risk of Healthcare Pathogen Transmission from Computer Keyboards","volume":"112","author":"Ledwoch","year":"2021","journal-title":"J. Hosp. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1086\/520737","article-title":"Widespread Environmental Contamination Associated with Patients with Diarrhea and Methicillin-Resistant Staphylococcus aureus Colonization of the Gastrointestinal Tract","volume":"28","author":"Boyce","year":"2007","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.jhin.2009.03.030","article-title":"The Role of Environmental Cleaning in the Control of Hospital-Acquired Infection","volume":"73","author":"Dancer","year":"2010","journal-title":"J. Hosp. Infect."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1038\/s41591-020-0894-4","article-title":"Cartography of Opportunistic Pathogens and Antibiotic Resistance Genes in a Tertiary Hospital Environment","volume":"26","author":"Chng","year":"2020","journal-title":"Nat. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1001\/archinte.166.18.1945","article-title":"Risk of Acquiring Antibiotic-Resistant Bacteria from Prior Room Occupants","volume":"166","author":"Huang","year":"2006","journal-title":"Arch. Intern. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1001\/archinternmed.2011.64","article-title":"Environmental Cleaning Intervention and Risk of Acquiring Multidrug-Resistant Organisms from Prior Room Occupants","volume":"171","author":"Datta","year":"2011","journal-title":"Arch. Intern. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/s44259-025-00137-9","article-title":"The Hospital Sink Drain Microbiome as a Melting Pot for AMR Transmission to Nosocomial Pathogens","volume":"3","author":"McCallum","year":"2025","journal-title":"npj Antimicrob. Resist."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.jhin.2022.04.017","article-title":"Impact of Sink Design on Bacterial Transmission from Hospital Sink Drains to the Surrounding Sink Environment Tested Using a Fluorescent Marker","volume":"127","author":"Pirzadian","year":"2022","journal-title":"J. Hosp. Infect."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/j.1469-0691.2009.03092.x","article-title":"Methods for Screening for Methicillin-Resistant Staphylococcus aureus Carriage","volume":"15","author":"French","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Tenover, F.C., and Tickler, I.A. (2022). Detection of Methicillin-Resistant Staphylococcus aureus Infections Using Molecular Methods. Antibiotics, 11.","DOI":"10.3390\/antibiotics11020239"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Hansen, S., and Abd El Wahed, A. (2020). Point-Of-Care or Point-Of-Need Diagnostic Tests: Time to Change Outbreak Investigation and Pathogen Detection. Trop. Med. Infect. Dis., 5.","DOI":"10.3390\/tropicalmed5040151"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5880","DOI":"10.1111\/1541-4337.12839","article-title":"Rapid point-of-need detection of bacteria and their toxins in food using gold nanoparticles","volume":"20","author":"Marin","year":"2021","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Sutarlie, L., Ow, S.Y., and Su, X.D. (2017). Nanomaterials-based Biosensors for Detection of Microorganisms and Microbial Toxins. Biotechnol. J., 12.","DOI":"10.1002\/biot.201500459"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"117630","DOI":"10.1016\/j.trac.2024.117630","article-title":"Optical Biosensors Utilising Viral Receptors ACE2 and ACE2 Mimics","volume":"173","author":"Ow","year":"2024","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"115758","DOI":"10.1016\/j.bios.2023.115758","article-title":"Argonaute-DNAzyme Tandem Biosensing for Highly Sensitive and Simultaneous Dual-Gene Detection of Methicillin-Resistant Staphylococcus aureus","volume":"244","author":"Li","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"134568","DOI":"10.1016\/j.snb.2023.134568","article-title":"Ultrasensitive Detection of Methicillin-Resistant Staphylococcus aureus Using a T7 Exonuclease-Assisted PAM-Free Dual CRISPR-Cas12a Biosensor","volume":"396","author":"Yang","year":"2023","journal-title":"Sens. Actuators B Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1801893","DOI":"10.1002\/smll.201801893","article-title":"A Hierarchical 3D Nanostructured Microfluidic Device for Sensitive Detection of Pathogenic Bacteria","volume":"14","author":"Jalali","year":"2018","journal-title":"Small"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"113189","DOI":"10.1016\/j.bios.2021.113189","article-title":"Lateral flow assay of Methicillin\u2014Resistant Staphylococcus aureus Using Bacteriophage Cellular Wall-binding Domain as Recognition Agent","volume":"182","author":"Yang","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1021\/acs.accounts.1c00598","article-title":"Noble Metal Nanoparticle Biosensors: From Fundamental Studies toward Point-of-Care Diagnostics","volume":"55","author":"Geng","year":"2022","journal-title":"Acc. Chem. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"17993","DOI":"10.1039\/D5NR01459J","article-title":"Nanoparticle-Based Biosensors for Virus Detection in Food Systems: From Farm to Fork","volume":"17","author":"Sarza","year":"2025","journal-title":"Nanoscale"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1021\/acsnano.8b06395","article-title":"Rapid Colorimetric Detection of Bacterial Species through the Capture of Gold Nanoparticles by Chimeric Phages","volume":"13","author":"Peng","year":"2019","journal-title":"ACS Nano"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"16675","DOI":"10.1039\/D3NR02635C","article-title":"A Rapid Total Bacteria Count Method using Nanoparticle Conjugated with Aptamer for Water Quality","volume":"15","author":"Sutarlie","year":"2023","journal-title":"Nanoscale"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6070","DOI":"10.1021\/acsanm.5c04984","article-title":"Rapid and Portable Gold Nanoparticle- Based Nanosensors for Vibrio spp. Detection from Aquaculture Water and Fish Tissues","volume":"9","author":"Aung","year":"2026","journal-title":"ACS Appl. Nano Mater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1089\/mab.2015.0013","article-title":"Generation and Characterization of Murine Monoclonal Antibodies anti-PBP2a of Methicillin-resistant Staphylococcus aureus","volume":"34","author":"Senna","year":"2015","journal-title":"Monoclon. Antib. Immunodiagn. Immunother."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5088","DOI":"10.1128\/AAC.00518-09","article-title":"Methicillin-resistant Staphylococcus aureus screening by online immunometric monitoring of bacterial growth under selective pressure","volume":"53","author":"Stenholm","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_40","unstructured":"Mathers, A.J., Lewis, J.S., Bryson, A.L., Alby, K., Bobenchik, A.M., Campeau, S., Dingle, T., Esparza, G., Fisher, M.A., and Lutgring, J. (2026). CLSI M100 Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. [36th ed.]."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1021\/ac00100a008","article-title":"Preparation and Characterization of Au Colloid Monolayers","volume":"67","author":"Grabar","year":"1995","journal-title":"Anal. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1139\/v80-055","article-title":"The Structure of Schiff Reagent Aldehyde Adducts and the Mechanism of the Schiff Reaction as Determined by Nuclear Magnetic Resonance Spectroscopy","volume":"58","author":"Robins","year":"1980","journal-title":"Can. J. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"The MetaSUB International Consortium (2016). The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium Inaugural Meeting Report. Microbiome, 4, 24. Erratum in Microbiome\u00a02016, 4, 45. https:\/\/doi.org\/10.1186\/s40168-016-0184-z.","DOI":"10.1186\/s40168-016-0184-z"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1021\/la202734f","article-title":"Comparative Study of Random and Oriented Antibody Immobilization as Measured by Dual Polarization Interferometry and Surface Plasmon Resonance Spectroscopy","volume":"28","author":"Song","year":"2012","journal-title":"Langmuir"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"8253","DOI":"10.1021\/acsomega.8b00591","article-title":"Quantifying Bound and Active Antibodies Conjugated to Gold Nanoparticles: A Comprehensive and Robust Approach to Evaluate Immobilization Chemistry","volume":"3","author":"Tripathi","year":"2018","journal-title":"ACS Omega"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1128\/JCM.00970-15","article-title":"Evaluation of BD Max StaphSR and BD Max MRSAXT Assays Using ESwab-Collected Specimens","volume":"53","author":"Silbert","year":"2015","journal-title":"J. Clin. Microbiol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/1940\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T09:08:35Z","timestamp":1788340115000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/1940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,2]]},"references-count":46,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["microorganisms14091940"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14091940","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,2]]}}}