{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:13:49Z","timestamp":1788527629969,"version":"build-2803163510"},"reference-count":45,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"Postdoctoral Foundation of China","doi-asserted-by":"publisher","award":["GZC20240430"],"award-info":[{"award-number":["GZC20240430"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"Postdoctoral Foundation of China","doi-asserted-by":"publisher","award":["2025M770267"],"award-info":[{"award-number":["2025M770267"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Feline calicivirus (FCV) is a highly heterogeneous pathogen and a major cause of feline upper respiratory tract disease, highlighting the need for continuous surveillance of its genetic diversity and pathogenic characteristics. In this study, we isolated and comprehensively characterized a newly isolated FCV strain, HN\/ZZ\/2025, from cats at a feline trading market in Zhengzhou, China, and systematically evaluated its molecular features, in vitro replication characteristics, physicochemical properties, and pathogenicity in cats. Phylogenetic analysis classified HN\/ZZ\/2025 as genotype GI. The isolate replicated efficiently in CRFK, F81, and Fc3Tg cells, reaching peak titers of 107.18, 107.50, and 105.89 TCID50\/0.1 mL, respectively, and exhibited typical calicivirus-like particles with diameters of 35\u201340 nm. Complete genome analysis showed that HN\/ZZ\/2025 shared the highest nucleotide and amino acid sequence identities of 85.7% and 62.8%, respectively, with the closely related Chinese FCV strain CH-JL4, and revealed distinct amino acid variations within the hypervariable E region of the VP1 capsid protein. Experimental infection of cats (n = 3\/group) resulted in pyrexia, with rectal temperatures reaching 39.8\u201340.2 \u00b0C, weight loss, oral ulceration, and persistent viral RNA shedding from 1 to 21 days post-infection. Viral RNA and VP1 antigen were detected in the lungs, trachea, kidneys, and spleen, indicating systemic dissemination and broad tissue distribution. Although no mortality occurred during the observation period, the observed clinical signs, viral dissemination, and histopathological lesions indicate that HN\/ZZ\/2025 is capable of causing clinically evident disease in experimentally infected cats. These findings provide useful insights into the molecular epidemiology, tissue tropism, and host\u2013virus interactions of circulating FCV strains and establish HN\/ZZ\/2025 as a useful isolate for future studies of FCV genetic diversity and pathogenesis.<\/jats:p>","DOI":"10.3390\/microorganisms14091958","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:20:46Z","timestamp":1788524446000},"page":"1958","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation, Molecular Characterization, and Pathogenicity Evaluation of a Genotype GI Feline Calicivirus Isolate"],"prefix":"10.3390","volume":"14","author":[{"given":"Jia-You","family":"Xing","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs, 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(2022). Special Issue: Viral Infections in Companion Animals. Viruses, 14.","DOI":"10.3390\/v14020320"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1016\/j.cvsm.2011.07.006","article-title":"Feline respiratory disease complex","volume":"41","author":"Cohn","year":"2011","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1053\/jfms.2000.0084","article-title":"A study of feline upper respiratory tract disease with reference to prevalence and risk factors for infection with feline calicivirus and feline herpesvirus","volume":"2","author":"Binns","year":"2000","journal-title":"J. Feline Med. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.vetmic.2006.04.004","article-title":"The challenge for the next generation of feline calicivirus vaccines","volume":"117","author":"Radford","year":"2006","journal-title":"Vet. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1292\/jvms.64.215","article-title":"An etiological investigation of domestic cats with conjunctivitis and upper respiratory tract disease in Japan","volume":"64","author":"Cai","year":"2002","journal-title":"J. Vet. Med. Sci."},{"key":"ref_6","first-page":"455","article-title":"Etiological investigation of multiple respiratory infections in cats","volume":"30","author":"Meridiani","year":"2007","journal-title":"New Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.tvjl.2015.08.001","article-title":"Detection of feline upper respiratory tract disease pathogens using a commercially available real-time PCR test","volume":"206","author":"Litster","year":"2015","journal-title":"Vet. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e0009325","DOI":"10.1128\/jvi.00093-25","article-title":"A novel replication-deficient FCV vaccine provides strong immune protection in cats","volume":"99","author":"Heng","year":"2025","journal-title":"J. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.virol.2018.11.017","article-title":"The feline calicivirus leader of the capsid protein causes survivin and XIAP downregulation and apoptosis","volume":"527","year":"2019","journal-title":"Virology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s13028-015-0178-z","article-title":"Ability of vaccine strain induced antibodies to neutralize field isolates of caliciviruses from Swedish cats","volume":"57","author":"Wensman","year":"2015","journal-title":"Acta Vet. Scand."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1016\/S0264-410X(97)00059-5","article-title":"The use of sequence analysis of a feline calicivirus (FCV) hypervariable region in the epidemiological investigation of FCV related disease and vaccine failures","volume":"15","author":"Radford","year":"1997","journal-title":"Vaccine"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Lanave, G., Buonavoglia, A., Pellegrini, F., Di Martino, B., Di Profio, F., Diakoudi, G., Catella, C., Omar, A.H., Vasinioti, V.I., and Cardone, R. (2023). An Outbreak of Limping Syndrome Associated with Feline Calicivirus. Animals, 13.","DOI":"10.3390\/ani13111778"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Hofmann-Lehmann, R., Hosie, M.J., Hartmann, K., Egberink, H., Truyen, U., Tasker, S., Bel\u00e1k, S., Boucraut-Baralon, C., Frymus, T., and Lloret, A. (2022). Calicivirus Infection in Cats. Viruses, 14.","DOI":"10.3390\/v14050937"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e73","DOI":"10.4142\/jvs.2021.22.e73","article-title":"Prevalence of feline calicivirus and the distribution of serum neutralizing antibody against isolate strains in cats of Hangzhou, China","volume":"22","author":"Zheng","year":"2021","journal-title":"J. Vet. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jfms.2009.08.006","article-title":"A dual-strain feline calicivirus vaccine stimulates broader cross-neutralization antibodies than a single-strain vaccine and lessens clinical signs in vaccinated cats when challenged with a homologous feline calicivirus strain associated with virulent systemic disease","volume":"12","author":"Huang","year":"2010","journal-title":"J. Feline Med. Surg."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Wei, Y., Zeng, Q., Gou, H., and Bao, S. (2024). Update on feline calicivirus: Viral evolution, pathogenesis, epidemiology, prevention and control. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1388420"},{"key":"ref_17","first-page":"225","article-title":"An Update on Feline Calicivirus","volume":"164","author":"Spiri","year":"2022","journal-title":"Schweiz. Arch. Fur Tierheilkd."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.cvsm.2008.03.002","article-title":"Molecular virology of feline calicivirus","volume":"38","author":"Pesavento","year":"2008","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Cao, L., Liu, J., Li, Y., Xie, D., Yan, Q., Li, Q., Cao, Y., Du, W., Li, J., and Ye, Z. (2023). Biological Characteristics of Feline Calicivirus Epidemic Strains in China and Screening of Broad-Spectrum Protective Vaccine Strains. Vaccines, 11.","DOI":"10.3390\/vaccines11121858"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Bordicchia, M., Fumian, T.M., Van Brussel, K., Russo, A.G., Carrai, M., Le, S.J., Pesavento, P.A., Holmes, E.C., Martella, V., and White, P. (2021). Feline Calicivirus Virulent Systemic Disease: Clinical Epidemiology, Analysis of Viral Isolates and In Vitro Efficacy of Novel Antivirals in Australian Outbreaks. Viruses, 13.","DOI":"10.20944\/preprints202108.0341.v1"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.rvsc.2019.02.008","article-title":"Feline calicivirus infection in cats with virulent systemic disease, Italy","volume":"124","author":"Caringella","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e51","DOI":"10.4142\/jvs.24030","article-title":"Outbreaks of nosocomial feline calicivirus-associated virulent systemic disease in Korea","volume":"25","author":"Park","year":"2024","journal-title":"J. Vet. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1099\/vir.0.82488-0","article-title":"Feline caliciviruses (FCVs) isolated from cats with virulent systemic disease possess in vitro phenotypes distinct from those of other FCV isolates","volume":"88","author":"Ossiboff","year":"2007","journal-title":"J. Gen. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S1098-612X(03)00008-1","article-title":"An epizootic of highly virulent feline calicivirus disease in a hospital setting in New England","volume":"5","author":"Poland","year":"2003","journal-title":"J. Feline Med. Surg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1051\/vetres:2006056","article-title":"Feline calicivirus","volume":"38","author":"Radford","year":"2007","journal-title":"Vet. Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Mao, J., Ye, S., Deng, J., Song, J., Wang, Z., Chen, A., Zhou, P., and Li, S. (2023). Feline Calicivirus P39 Inhibits Innate Immune Responses by Autophagic Degradation of Retinoic Acid Inducible Gene I. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065254"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7060","DOI":"10.1128\/JVI.76.14.7060-7072.2002","article-title":"Processing map and essential cleavage sites of the nonstructural polyprotein encoded by ORF1 of the feline calicivirus genome","volume":"76","author":"Sosnovtsev","year":"2002","journal-title":"J. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1006\/viro.2000.0579","article-title":"Identification and genomic mapping of the ORF3 and VPg proteins in feline calicivirus virions","volume":"277","author":"Sosnovtsev","year":"2000","journal-title":"Virology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e0035024","DOI":"10.1128\/jvi.00350-24","article-title":"VP2 mediates the release of the feline calicivirus RNA genome by puncturing the endosome membrane of infected cells","volume":"98","author":"Sun","year":"2024","journal-title":"J. Virol."},{"key":"ref_30","first-page":"382","article-title":"A new feline virus isolated in tissue culture","volume":"18","author":"Fastier","year":"1957","journal-title":"Am. J. Vet. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jfms.2004.07.004","article-title":"Epidemiologic evaluation of multiple respiratory pathogens in cats in animal shelters","volume":"7","author":"Bannasch","year":"2005","journal-title":"J. Feline Med. Surg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1136\/vr.103446","article-title":"European molecular epidemiology and strain diversity of feline calicivirus","volume":"178","author":"Hou","year":"2016","journal-title":"Vet. Rec."},{"key":"ref_33","first-page":"141","article-title":"A strain of calicivirus isolated from lions with vesicular lesions on tongue and snout","volume":"20","author":"Kadoi","year":"1997","journal-title":"New Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.1016\/j.vaccine.2017.03.030","article-title":"A multi-national European cross-sectional study of feline calicivirus epidemiology, diversity and vaccine cross-reactivity","volume":"35","author":"Afonso","year":"2017","journal-title":"Vaccine"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.vetmic.2016.07.005","article-title":"Molecular characterization of a feline calicivirus isolated from tiger and its pathogenesis in cats","volume":"192","author":"Tian","year":"2016","journal-title":"Vet. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.vetmic.2015.10.015","article-title":"Molecular characterization and virus neutralization patterns of severe, non-epizootic forms of feline calicivirus infections resembling virulent systemic disease in cats in Switzerland and in Liechtenstein","volume":"182","author":"Willi","year":"2016","journal-title":"Vet. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0378-1135(00)00183-8","article-title":"An isolated epizootic of hemorrhagic-like fever in cats caused by a novel and highly virulent strain of feline calicivirus","volume":"73","author":"Pedersen","year":"2000","journal-title":"Vet. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1023\/A:1015253621079","article-title":"Phylogenetic analysis of field isolates of feline calcivirus (FCV) in Japan by sequencing part of its capsid gene","volume":"26","author":"Sato","year":"2002","journal-title":"Vet. Res. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1051\/vetres:2006063","article-title":"Feline herpesvirus","volume":"38","author":"Gaskell","year":"2007","journal-title":"Vet. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1016\/j.ajic.2015.06.021","article-title":"Efficacy of common disinfectant\/cleaning agents in inactivating murine norovirus and feline calicivirus as surrogate viruses for human norovirus","volume":"43","author":"Chiu","year":"2015","journal-title":"Am. J. Infect. Control"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"231","DOI":"10.5326\/0380231","article-title":"Virucidal efficacy of four new disinfectants","volume":"38","author":"Eleraky","year":"2002","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1099\/jgv.0.001332","article-title":"ICTV Virus Taxonomy Profile: Caliciviridae","volume":"100","author":"Estes","year":"2019","journal-title":"J. Gen. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1099\/0022-1317-78-2-303","article-title":"Mapping of antigenic sites involved in neutralization on the capsid protein of feline calicivirus","volume":"78","author":"Tohya","year":"1997","journal-title":"J. Gen. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s13567-020-00785-x","article-title":"Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies","volume":"51","author":"Angulo","year":"2020","journal-title":"Vet. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1354\/vp.41-3-257","article-title":"Pathologic, immunohistochemical, and electron microscopic findings in naturally occurring virulent systemic feline calicivirus infection in cats","volume":"41","author":"Pesavento","year":"2004","journal-title":"Vet. Pathol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/1958\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:21:23Z","timestamp":1788524483000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/1958"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":45,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["microorganisms14091958"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14091958","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]}}}