{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:07:30Z","timestamp":1788944850052,"version":"build-2803163510"},"reference-count":54,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:00:00Z","timestamp":1788652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006229","name":"Oak Ridge Institute for Science and Education","doi-asserted-by":"publisher","award":["DE-SC0014664"],"award-info":[{"award-number":["DE-SC0014664"]}],"id":[{"id":"10.13039\/100006229","id-type":"DOI","asserted-by":"publisher"}]},{"award":["DE-SC0014664"],"award-info":[{"award-number":["DE-SC0014664"]}],"id":[{"id":"https:\/\/ror.org\/040vxhp34","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000199","name":"USDA Agricultural Research Service","doi-asserted-by":"publisher","award":["5090-31000-026-00-D"],"award-info":[{"award-number":["5090-31000-026-00-D"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000199","name":"USDA Agricultural Research Service","doi-asserted-by":"publisher","award":["5090-31000-025-00D"],"award-info":[{"award-number":["5090-31000-025-00D"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"award":["5090-31000-026-00-D"],"award-info":[{"award-number":["5090-31000-026-00-D"]}],"id":[{"id":"https:\/\/ror.org\/02d2m2044","id-type":"ROR","asserted-by":"publisher"}]},{"award":["5090-31000-025-00D"],"award-info":[{"award-number":["5090-31000-025-00D"]}],"id":[{"id":"https:\/\/ror.org\/02d2m2044","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"USDA National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["WIS02007"],"award-info":[{"award-number":["WIS02007"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"award":["WIS02007"],"award-info":[{"award-number":["WIS02007"]}],"id":[{"id":"https:\/\/ror.org\/05qx3fv49","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Ruminants harbor a ruminal microbiome that converts their host-indigestible diet into nutrients. This microbiome contains three distinct communities: liquid, solid, and epithelial (or epimural). Currently, there is limited research examining the diet-dependent responses of all three microbiomes within the same animal. Here, we used next-generation 16S rRNA sequencing to characterize the ruminal solid (RS), liquid (RL), and epimural (RE) microbiotas of 13 lactating and cannulated Holstein dairy cows fed either a high- or low-starch diet in a crossover experimental design. Independent of diet, we found that all sample types were dominated by the phyla Firmicutes and Bacteroidetes. Proteobacteria and Epsilonbacteraeota were also highly abundant but only in the RE. Although the total VFA molar abundance did not differ between diets, propionate and valerate were found to increase with the high-starch diet, while acetate was increased with the low-starch diet. Overall, we found that the RE microbiota was more diverse than the RS and RL communities, with diet impacting community diversity in the RS. We found that sample type, diet treatment, and their interaction significantly impacted community structure and composition. Notably, Prevotella was most abundant in the RL and RS, particularly on the low-starch diet. We also found that Lachnospiraceae were significantly enriched in the RS and RL with a high-starch diet, whereas Succiniclasticum was highly abundant in the RE with a low-starch diet. These data suggest that diet influences all three ruminal microbiomes and provides a useful framework for understanding the role of these microbiomes in mediating host production.<\/jats:p>","DOI":"10.3390\/microorganisms14091968","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T08:07:06Z","timestamp":1788768426000},"page":"1968","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating the Impact of High- and Low-Starch Diets on the Ruminal Microbiota of Dairy Cows"],"prefix":"10.3390","volume":"14","author":[{"given":"Dino L.","family":"Sbardellati","sequence":"first","affiliation":[{"name":"Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4694-822X","authenticated-orcid":false,"given":"Amelie","family":"Fischer","sequence":"additional","affiliation":[{"name":"Oak Ridge Institute for Science and Education, Oak Ridge, TN 37830, USA"},{"name":"US Dairy Forage Research Center, USDA-Agricultural Research Service, Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7092-2754","authenticated-orcid":false,"given":"Madison S.","family":"Cox","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA"},{"name":"Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI 53706, USA"},{"name":"Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, WA 98109, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Wenli","family":"Li","sequence":"additional","affiliation":[{"name":"US Dairy Forage Research Center, USDA-Agricultural Research Service, Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5290-3602","authenticated-orcid":false,"given":"Kenneth F.","family":"Kalscheur","sequence":"additional","affiliation":[{"name":"US Dairy Forage Research Center, USDA-Agricultural Research Service, Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6170-711X","authenticated-orcid":false,"given":"Garret","family":"Suen","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,6]]},"reference":[{"key":"ref_1","unstructured":"Dehority, B.A. (2003). Rumen Microbiology, Nottingham University Press."},{"key":"ref_2","unstructured":"Hungate, R.E. (2003). The Rumen Microbial Ecosystem, Academic Press."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Millen, D.D., Beni Arrigoni, M., and Pacheco, R.D.L. (2016). Rumenology, Springer International Publishing.","DOI":"10.1007\/978-3-319-30533-2"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/nrmicro1817","article-title":"Polysaccharide Utilization by Gut Bacteria: Potential for New Insights from Genomic Analysis","volume":"6","author":"Flint","year":"2008","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1152\/physrev.1990.70.2.567","article-title":"Energy Contributions of Volatile Fatty Acids from the Gastrointestinal Tract in Various Species","volume":"70","author":"Bergman","year":"1990","journal-title":"Physiol. Rev."},{"key":"ref_6","first-page":"92","article-title":"16S rDNA Analysis of Bacterial Diversity in Three Fractions of Cow Rum","volume":"16","author":"Jeong","year":"2006","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"16116","DOI":"10.1038\/srep16116","article-title":"Characterising the Bacterial Microbiota across the Gastrointestinal Tracts of Dairy Cattle: Membership and Potential Function","volume":"5","author":"Mao","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3636","DOI":"10.3168\/jds.2019-17649","article-title":"The Bovine Epimural Microbiota Displays Compositional and Structural Heterogeneity across Different Ruminal Locations","volume":"103","author":"Sbardellati","year":"2020","journal-title":"J. Dairy Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Pac\u00edfico, C., Petri, R.M., Ricci, S., Mickdam, E., Wetzels, S.U., Neubauer, V., and Zebeli, Q. (2021). Unveiling the Bovine Epimural Microbiota Composition and Putative Function. Microorganisms, 9.","DOI":"10.3390\/microorganisms9020342"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/ajcn\/32.1.139","article-title":"Adherent Epithelial Bacteria in Ruminants and Their Roles in Digestive Tract Function","volume":"32","author":"Cheng","year":"1979","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1128\/aem.39.5.1059-1066.1980","article-title":"Digestion of Epithelial Tissue of the Rumen Wall by Adherent Bacteria in Infused and Conventionally Fed Sheep","volume":"39","author":"Dinsdale","year":"1980","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1038\/279424a0","article-title":"An Independent Microbial Flora of the Epithelium and Its Role in the Ecomicrobiology of the Rumen","volume":"279","author":"Wallace","year":"1979","journal-title":"Nature"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.aninu.2022.04.002","article-title":"Understanding the Role of Rumen Epithelial Host-Microbe Interactions in Cattle Feed Efficiency","volume":"10","author":"Na","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4377","DOI":"10.3168\/jds.2016-11473","article-title":"Factors Influencing Ruminal Bacterial Community Diversity and Composit and Microbial Fibrolytic Enzyme Abundance in Lactating Dairy Cows with a Focus on the Role of Active Dry Yeast","volume":"100","author":"AlZahal","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.3168\/jds.2016-11620","article-title":"Epimural Bacterial Community Structure in the Rumen of Holstein Cows W Different Responses to a Long-Term Subacute Ruminal Acidosis Diet Challenge","volume":"100","author":"Wetzels","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"100599","DOI":"10.1016\/j.animal.2022.100599","article-title":"Feed Efficiency of Lactating Holstein Cows Is Repeatable within Diet but Less Reproducible When Changing Dietary Starch and Forage Concentrations","volume":"16","author":"Fischer","year":"2022","journal-title":"Animal"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1007\/BF00164416","article-title":"A Segmented Gas\/Liquid Delivery System for Continuous Culture of Microorganisms on Insoluble Substrates and Its Use for Growth of Ruminococc flavefaciens on Cellulose","volume":"36","author":"Weimer","year":"1991","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4658","DOI":"10.1111\/mec.12884","article-title":"Hibernation Alters the Diversity and Composition of Mucosa-Associated Bacteria While Enhancing Antimicrobial Defence in the Gut of 13-Lined Ground Squirrels","volume":"23","author":"Neil","year":"2014","journal-title":"Mol. Ecol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"40864","DOI":"10.1038\/srep40864","article-title":"Microbial Succession in the Gastrointestinal Tract of Dairy Cows from 2 Weeks to First Lactation","volume":"7","author":"Breaker","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.mimet.2005.01.011","article-title":"Evaluation of DNA Extraction Methods from Mouse Stomachs for the Quantification of H. Pylori by Real-Time PCR","volume":"62","author":"Roussel","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5112","DOI":"10.1128\/AEM.01043-13","article-title":"Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the Miseq Illumina Sequencing Platform","volume":"79","author":"Kozich","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing Mothur: Open-Source, Platform-Independent, Community-Suppo Software for Describing and Comparing Microbial Communities","volume":"75","author":"Schloss","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA Ribosomal RNA Gene Database Project: Improved Data Processing and Web-Based Tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1093\/biomet\/43.1-2.45","article-title":"The Number of New Species, and the Increase in Population Coverage, When a Sample Is Increased","volume":"43","author":"Good","year":"1956","journal-title":"Biometrika"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1002\/j.1538-7305.1948.tb01338.x","article-title":"A Mathematical Theory of Communication","volume":"27","author":"Shannon","year":"1948","journal-title":"Bell Syst. Tech. J."},{"key":"ref_26","first-page":"265","article-title":"Non-Parametric Estimation of the Number of Classes in a Population","volume":"11","author":"Chao","year":"1984","journal-title":"Scand. J. Stat."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v067.i01","article-title":"Fitting Linear Mixed-Effect Models Using {lme4}","volume":"67","author":"Bates","year":"2015","journal-title":"J. Stat. Softw."},{"key":"ref_28","unstructured":"Oksanen, A.J., Blanchet, F.G., Kindt, R., Legendre, P., Minchin, P.R., Hara, R.B.O., Simpson, G.L., Solymos, P., Stevens, M.H.H., and Wagner, H. (2015). Veganl: Community Ecology Package. R Package Vegan, version 2.2-1, Comprehensive R Archive Network (CRAN)."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"291","DOI":"10.22358\/jafs\/68938\/1999","article-title":"A Review of Starch Digestion in the Lactating Dairy Cow and Proposals for a Mechanistic Model: 1. Dietary Starch Characterisation and Rumina Starch Digestion","volume":"8","author":"Mills","year":"1999","journal-title":"J. Anim. Feed Sci."},{"key":"ref_30","unstructured":"Hungate, R.E. (2013). The Rumen and Its Microbes, Elsevier."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1051\/animres:19960201","article-title":"Metabolites, Water and Mineral Exchanges across the Rumen Wall: Mechan and Regulation","volume":"45","author":"Meschy","year":"1996","journal-title":"Anim. Res."},{"key":"ref_32","first-page":"461","article-title":"Rumen Metabolism","volume":"57","author":"Baldwin","year":"1983","journal-title":"J. Anim. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.anifeedsci.2011.12.005","article-title":"Ruminal PH Regulation and Nutritional Consequences of Low PH","volume":"172","author":"Dijkstra","year":"2012","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7115","DOI":"10.1128\/AEM.00739-09","article-title":"Rumen Microbiome Composition Determined Using Two Nutritional Models of Subacute Ruminal Acidosis","volume":"75","author":"Khafipour","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.anaerobe.2013.08.003","article-title":"Impact of Subacute Ruminal Acidosis (SARA) Adaptation on Rumen Microbi in Dairy Cattle Using Pyrosequencing","volume":"24","author":"Mao","year":"2013","journal-title":"Anaerobe"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"14567","DOI":"10.1038\/srep14567","article-title":"Rumen Microbial Community Composition Varies with Diet and Host, but a Core Microbiome Is Found across a Wide Geographical Range","volume":"5","author":"Henderson","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1007\/s00248-009-9609-6","article-title":"Rumen Bacterial Diversity Dynamics Associated with Changing from Bermu Hay to Grazed Winter Wheat Diets","volume":"59","author":"Pitta","year":"2010","journal-title":"Microb. Ecol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.1128\/AEM.67.6.2766-2774.2001","article-title":"Diet-Dependent Shifts in the Bacterial Population of the Rumen Reveale with Real-Time PCR","volume":"67","author":"Tajima","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Li, R.W., Wu, S., Baldwin, R.L., Li, W., and Li, C. (2012). Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0029392"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1093\/jn\/122.1.178","article-title":"Starch Hydrolysis by the Ruminal Microflora","volume":"122","author":"Kotarski","year":"1992","journal-title":"J. Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1111\/apha.12155","article-title":"Bicarbonate-Dependent Transport of Acetate and Butyrate across the Basolateral Membrane of Sheep Rumen Epithelium","volume":"210","author":"Dengler","year":"2014","journal-title":"Acta Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1099\/00207713-40-2-205","article-title":"Prevotella, a New Genus To Include Bacteroides melaninogenicus and Related Species Formerly Classified in the Genus Bacteroides","volume":"40","author":"Shah","year":"1990","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s00253-006-0802-y","article-title":"Dominance of Prevotella and Low Abundance of Classical Ruminal Bacterial Species in the Bovine Rumen Revealed by Relative Quantification Real-Time PCR","volume":"75","author":"Stevenson","year":"2007","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1099\/00207713-47-2-284","article-title":"Phenotypic Diversity among Ruminal Isolates of Prevotella ruminicola: Proposal of Prevotella brevis Sp. Nov., Prevotella bryantii Sp. Nov., and Prevotella albensis Sp. Nov. and Redefinition of Prevotella rumini","volume":"47","author":"Wallace","year":"1997","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4269","DOI":"10.1007\/s00253-017-8150-7","article-title":"Fermentation of Model Hemicelluloses by Prevotella Strains and Butyrivibrio fibrisolvens in Pure Culture and in Ruminal Enrichment Cultures","volume":"101","author":"Emerson","year":"2017","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nbt.4110","article-title":"Cultivation and Sequencing of Rumen Microbiome Members from the Hungat Collection","volume":"36","author":"Seshadri","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"ref_47","unstructured":"Russell, J.B. (2002). Rumen Microbiology and Its Role in Ruminant Nutrition, Cornell University."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/BF00416597","article-title":"Enrichment and Isolation of Acetitomaculum ruminis, Gen. Nov., Sp. Nov.: Acetogenic Bacteria from the Bovine Rumen","volume":"151","author":"Greening","year":"1989","journal-title":"Arch. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3429","DOI":"10.1128\/AEM.64.9.3429-3436.1998","article-title":"Assessment of Reductive Acetogenesis with Indigenous Ruminal Bacterium Populations and Acetitomaculum ruminis","volume":"64","author":"Van","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1099\/ijsem.0.001615","article-title":"Desulfobulbus oligotrophicus Sp. Nov., a Sulfate-Reducing and Propiona","volume":"67","author":"Houari","year":"2017","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1007\/BF00411187","article-title":"Studies on Dissimilatory Sulfate-Reducing Bacteria That Decompose Fatt Acids II. Incomplete Oxidation of Propionate by Desulfobulbus propio Gen. Nov., Sp. Nov.","volume":"131","author":"Widdel","year":"1982","journal-title":"Arch. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1007\/BF02525315","article-title":"Elemental Sulfur as an Intermediate of Sulfide Oxidation with Oxygen by Desulfobulbus propionicus","volume":"164","author":"Fuseler","year":"1995","journal-title":"Arch. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1099\/00207713-45-2-297","article-title":"Succiniclasticum ruminis Gen. Nov., Sp. Nov., a Ruminal Bacterium Converting Succinate to Propionate as the Sole Energy-Yielding Mechanism","volume":"45","author":"Gylswyk","year":"1995","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.1128\/AEM.03983-12","article-title":"Changes in the Rumen Epimural Bacterial Diversity of Beef Cattle as Affected by Diet and Induced Ruminal Acidosis","volume":"79","author":"Petri","year":"2013","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/1968\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:25:11Z","timestamp":1788942311000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/1968"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,6]]},"references-count":54,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["microorganisms14091968"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14091968","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,6]]}}}