{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:09:03Z","timestamp":1788858543685,"version":"build-2803163510"},"reference-count":52,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009590","name":"College Teachers Innovation Fund of Gansu Provincial Education Department","doi-asserted-by":"publisher","award":["2026A-210"],"award-info":[{"award-number":["2026A-210"]}],"id":[{"id":"10.13039\/501100009590","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2026A-210"],"award-info":[{"award-number":["2026A-210"]}],"id":[{"id":"https:\/\/ror.org\/03w2z6524","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015810","name":"Longdong University Doctoral Research Fund","doi-asserted-by":"publisher","award":["XYBYZK2507"],"award-info":[{"award-number":["XYBYZK2507"]}],"id":[{"id":"10.13039\/501100015810","id-type":"DOI","asserted-by":"publisher"}]},{"award":["XYBYZK2507"],"award-info":[{"award-number":["XYBYZK2507"]}],"id":[{"id":"https:\/\/ror.org\/03wcn4h12","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015810","name":"Longdong University Joint Research Fund","doi-asserted-by":"publisher","award":["HXZK2528"],"award-info":[{"award-number":["HXZK2528"]}],"id":[{"id":"10.13039\/501100015810","id-type":"DOI","asserted-by":"publisher"}]},{"award":["HXZK2528"],"award-info":[{"award-number":["HXZK2528"]}],"id":[{"id":"https:\/\/ror.org\/03wcn4h12","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Qingyang City Science and Technology Planning Program","award":["QY-STK-2022B-104"],"award-info":[{"award-number":["QY-STK-2022B-104"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Oilfield microbiome studies have extensively characterized microbial community composition or isolated individual hydrocarbon degraders, but integrated exploration of cultivable functional bacteria from paired crude-oil and produced-water environments remains limited, particularly for strains combining hydrocarbon utilization with stress-tolerance and biosurfactant-related traits. In this study, paired crude-oil and produced-water samples from the Huoshaoshan Oilfield were investigated by combining 16S rRNA amplicon sequencing with culture-dependent isolation and functional screening. A total of 135 bacterial isolates representing 22 genera were recovered, of which 70 showed hydrocarbon-utilizing potential in primary screening. The collection also included isolates capable of growth at 10% NaCl and up to 55 \u00b0C. Comparison of sequencing and cultivation revealed marked differences between environmental abundance and recoverable functional resources. Representative isolates showed distinct removal profiles toward n-tetracosane (C24), naphthalene, phenanthrene, and fluoranthene under saline conditions, with Halomonas nitritophilus Ff1 and Halomonas sp. Fc15 showing particularly strong performance. Sequence-confirmed alkB and PAH-RHD\u03b1 fragments provided additional molecular support for the observed hydrocarbon-removal phenotypes. A three-strain consortium composed of Sphingomonas sp. Fa24, Halomonas nitritophilus Ff1, and Stutzerimonas stutzeri Ff2 achieved 87.4% C24 removal, exceeding the corresponding monocultures. These findings link oilfield microbial community profiling with cultivable functional resource mining and provide candidate strains for saline petroleum bioremediation.<\/jats:p>","DOI":"10.3390\/microorganisms14091981","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T08:10:22Z","timestamp":1788855022000},"page":"1981","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Analysis of Paired Crude Oil and Produced Water Reveals Stress-Tolerant Hydrocarbon-Degrading Bacteria with Bioremediation Potential"],"prefix":"10.3390","volume":"14","author":[{"given":"Ning","family":"Mao","sequence":"first","affiliation":[{"name":"Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration in Longdong, School of Agriculture and Bioengineering, Longdong University, Qingyang 745000, 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