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Here, the wild-type strain of Synechococcus sp. PCC 7002 and Dppk, a modified ppk-expression strain derived from the WT background, were subjected to phosphate starvation followed by phosphate resupply. Dense RT-qPCR sampling was used to resolve rapid ppk-expression dynamics, and time-resolved RNA sequencing was performed at seven stages with four biological replicates per condition. WT displayed rapid, stage-dependent changes in ppk expression, including a pronounced late induction after phosphate resupply, whereas Dppk showed a more moderate temporal pattern. Transcriptome-wide analyses further revealed distinct basal states and response trajectories between the strains. Functional analyses linked these differences to photosynthesis-related processes, phosphate transport, and polyphosphate metabolism. These findings indicate that refactoring ppk expression alters both target-gene kinetics and the temporal organization of the broader phosphate-responsive transcriptional network.<\/jats:p>","DOI":"10.3390\/microorganisms14091997","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T12:26:11Z","timestamp":1788956771000},"page":"1997","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modified ppk Expression Alters Temporal Transcriptional and Phosphorus\u2013Nitrogen Responses to Phosphate Fluctuation in Synechococcus sp. PCC 7002"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8191-6609","authenticated-orcid":false,"given":"Sijia","family":"Shen","sequence":"first","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4902-6582","authenticated-orcid":false,"given":"Enci","family":"Ye","sequence":"additional","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Tao","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"Key Laboratory of Algal Biology, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"given":"Zi","family":"Ye","sequence":"additional","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"Key Laboratory of Algal Biology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"State Key Laboratory of Lake and Watershed Science for Water Security, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1146\/annurev-marine-010213-135046","article-title":"Microbially Mediated Transformations of Phosphorus in the Sea: New Views of an Old Cycle","volume":"6","author":"Karl","year":"2014","journal-title":"Annu. 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