{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T09:05:21Z","timestamp":1789376721106,"version":"build-2803163510"},"reference-count":47,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T00:00:00Z","timestamp":1789257600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of the Higher Education Institutions of Anhui Province","award":["2024AH051352"],"award-info":[{"award-number":["2024AH051352"]}]},{"name":"National Natural Science Foundation of the Higher Education Institutions of Anhui Province","award":["2025AHGXZK31487"],"award-info":[{"award-number":["2025AHGXZK31487"]}]},{"name":"National Natural Science Foundation of the Higher Education Institutions of Anhui Province","award":["2025AHGXZK31502"],"award-info":[{"award-number":["2025AHGXZK31502"]}]},{"award":["2024AH051352"],"award-info":[{"award-number":["2024AH051352"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2025AHGXZK31487"],"award-info":[{"award-number":["2025AHGXZK31487"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2025AHGXZK31502"],"award-info":[{"award-number":["2025AHGXZK31502"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Chizhou College High-Level Talent Research Start up Fund","award":["CZ2024YJRC37"],"award-info":[{"award-number":["CZ2024YJRC37"]}]},{"name":"Chizhou College High-Level Talent Research Start up Fund","award":["CZ2025YJRC153"],"award-info":[{"award-number":["CZ2025YJRC153"]}]},{"award":["CZ2024YJRC37"],"award-info":[{"award-number":["CZ2024YJRC37"]}],"id":[{"id":"https:\/\/ror.org\/007cx7r28","id-type":"ROR","asserted-by":"publisher"}]},{"award":["CZ2025YJRC153"],"award-info":[{"award-number":["CZ2025YJRC153"]}],"id":[{"id":"https:\/\/ror.org\/007cx7r28","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Natural Students\u2019 Innovation and Entrepreneurship Training Program","award":["202511306005"],"award-info":[{"award-number":["202511306005"]}]},{"name":"Natural Students\u2019 Innovation and Entrepreneurship Training Program","award":["202611306001"],"award-info":[{"award-number":["202611306001"]}]},{"name":"Natural Students\u2019 Innovation and Entrepreneurship Training Program","award":["202611306004"],"award-info":[{"award-number":["202611306004"]}]},{"name":"Natural Students\u2019 Innovation and Entrepreneurship Training Program","award":["202611306018X"],"award-info":[{"award-number":["202611306018X"]}]},{"name":"Natural Students\u2019 Innovation and Entrepreneurship Training Program","award":["202611306040"],"award-info":[{"award-number":["202611306040"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Underground coal extraction alters aquatic ecosystems. Quantitative evaluation of microbial responses serves as an indicator for ecological security. High-throughput amplicon sequencing and structural equation modeling were applied to analyze aquatic bacterial and fungal communities before and after operations at the Talahao coal mine. Mining activities correlated with altered physicochemical water properties and reduced microbial community evenness. Environmental variables, including ion accumulation and suspended solid concentrations, mediated the indirect effects of mining on microbial restructuring based on structural equation models. Taxonomic composition shifted across domains following extraction. Pre-mining bacterial communities contained higher proportions of the genera Devosia and Acidovorax. Post-mining bacterial communities experienced an enrichment of the genus Malikia and the family Methylomonadaceae. Pre-mining fungal communities included Cladosporium and Aspergillus. Post-mining fungal communities exhibited a subterranean response pattern dominated by unclassified lineages and taxa belonging to Basidiobolaceae diverging from surface soil reference frameworks. Functional predictions indicated divergent stability between the two domains. Bacterial inferred functional profiles remained stable despite taxonomic restructuring. Fungal assemblages underwent compositional shifts in trophic guilds. These data provide a baseline regarding the divergent functional stability between bacterial and fungal communities in water bodies before and after underground coal extraction and establish biological indicators for environmental assessment.<\/jats:p>","DOI":"10.3390\/microorganisms14092041","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T08:10:23Z","timestamp":1789373423000},"page":"2041","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bacterial and Fungal Community Shifts and Environmental Drivers in Talahao Coal Mine Water"],"prefix":"10.3390","volume":"14","author":[{"given":"Limin","family":"Yang","sequence":"first","affiliation":[{"name":"School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youkun","family":"Huang","sequence":"additional","affiliation":[{"name":"Anhui Provincial Key Laboratory for Quality and Safety of Agri-Products, School of Resource and Environment, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duoen","family":"Gu","sequence":"additional","affiliation":[{"name":"School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Gu","sequence":"additional","affiliation":[{"name":"School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyu","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danyang","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiting","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuqing","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaowen","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China"},{"name":"Anhui Provincial Key Laboratory for Quality and Safety of Agri-Products, School of Resource and Environment, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.ijmst.2018.06.005","article-title":"Intelligent and ecological coal mining as well as clean utilization technology in China: Review and prospects","volume":"29","author":"Wang","year":"2019","journal-title":"Int. J. Min. Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"111623","DOI":"10.1016\/j.jenvman.2020.111623","article-title":"Evaluating the environmental and economic impact of mining for post-mined land restoration and land-use: A review","volume":"279","author":"Worlanyo","year":"2021","journal-title":"J. Environ. Manag."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Fashola, M.O., Ngole-Jeme, V.M., and Babalola, O.O. (2016). Heavy Metal Pollution from Gold Mines: Environmental Effects and Bacterial Strategies for Resistance. Int. J. Environ. Res. Public Health, 13.","DOI":"10.3390\/ijerph13111047"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"128032","DOI":"10.1016\/j.jhazmat.2021.128032","article-title":"Multiple heavy metal distribution and microbial community characteristics of vanadium-titanium magnetite tailing profiles under different management modes","volume":"429","author":"Gan","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"146193","DOI":"10.1016\/j.scitotenv.2021.146193","article-title":"Land subsidence: A global challenge","volume":"778","author":"Hosseini","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Ma, K., Zhang, Y.X., Ruan, M.Y., Guo, J., and Chai, T.Y. (2019). Land subsidence in a coal mining area reduced soil fertility and led to soil degradation in arid and semi-arid regions. Int. J. Environ. Res. Public Health, 16.","DOI":"10.3390\/ijerph16203929"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"118996","DOI":"10.1016\/j.envpol.2022.118996","article-title":"Properties of sediment dissolved organic matter respond to eutrophication and interact with bacterial communities in a plateau lake","volume":"301","author":"Li","year":"2022","journal-title":"Environ. Pollut."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Leuko, S., Koskinen, K., Sanna, L., D\u2019Angeli, I.M., De Waele, J., Marcia, P., Moissl-Eichinger, C., and Rettberg, P. (2017). The influence of human exploration on the microbial community structure and ammonia oxidizing potential of the Su Bentu limestone cave in Sardinia, Italy. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0180700"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"11512","DOI":"10.1073\/pnas.0801925105","article-title":"Resistance, resilience, and redundancy in microbial communities","volume":"105","author":"Allison","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1073\/pnas.0507535103","article-title":"The diversity and biogeography of soil bacterial communities","volume":"103","author":"Fierer","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"130063","DOI":"10.1016\/j.jhazmat.2022.130063","article-title":"Soil bacterial community structure in the habitats with different levels of heavy metal pollution at an abandoned polymetallic mine","volume":"442","author":"Yin","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"13509","DOI":"10.1007\/s11356-024-33377-9","article-title":"Comprehensive assessment of the microbial community structure in a typical lead-zinc mine soil","volume":"32","author":"Zeng","year":"2025","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zhang, L., Xu, Z.M., Sun, Y.J., Gao, Y.T., and Zhu, L.L. (2022). Coal mining activities driving the changes in microbial community and hydrochemical characteristics of underground mine water. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph192013359"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Xu, Q., Tan, D.H., Liu, J.Q., Qiu, H.F., and Yang, Y. (2025). Microbial community structure and potential functional diversity in a post-mining ecosystem of central China. Front. Environ. Sci., 13.","DOI":"10.3389\/fenvs.2025.1590925"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"15784","DOI":"10.1038\/s41598-022-20148-0","article-title":"Bacterial diversity in surface sediments of collapsed lakes in Huaibei, China","volume":"12","author":"Shen","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"174822","DOI":"10.1016\/j.scitotenv.2024.174822","article-title":"Variations in bacterial community succession and assembly mechanisms with mine age across various habitats in coal mining subsidence water areas","volume":"948","author":"Li","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"106103","DOI":"10.1016\/j.aquatox.2022.106103","article-title":"Exploring the microbial community inhabiting the phosphogypsum stacks of Huelva (SW SPAIN) by a high throughput 16S\/18S rDNA sequencing approach","volume":"245","author":"Guerrero","year":"2022","journal-title":"Aquat. Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"120866","DOI":"10.1016\/j.envpol.2022.120866","article-title":"Spatial distributions of microbial diversity in the contaminated deep groundwater: A case study of the Huaibei coalfield","volume":"318","author":"Chen","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: Fast length adjustment of short reads to improve genome assemblies","volume":"27","author":"Magoc","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: Highly accurate OTU sequences from microbial amplicon reads","volume":"10","author":"Edgar","year":"2013","journal-title":"Nat. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5261","DOI":"10.1128\/AEM.00062-07","article-title":"Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy","volume":"73","author":"Wang","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"117379","DOI":"10.1016\/j.envres.2023.117379","article-title":"Effects of the polyhalogenated carbazoles 3-bromocarbazole and 1,3,6,8-tetrabromocarbazole on soil microbial communities","volume":"239","author":"Shi","year":"2023","journal-title":"Environ. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1029\/2018JG004670","article-title":"Microbial Community Responses to Vanadium Distributions in Mining Geological Environments and Bioremediation Assessment","volume":"124","author":"Zhang","year":"2019","journal-title":"J. Geophys. Res.-Biogeosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1038\/s41396-020-0632-4","article-title":"Novel phosphate-solubilizing bacteria enhance soil phosphorus cycling following ecological restoration of land degraded by mining","volume":"14","author":"Liang","year":"2020","journal-title":"ISME J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"108826","DOI":"10.1016\/j.soilbio.2022.108826","article-title":"Distribution of phosphorus cycling genes across land uses and microbial taxonomic groups based on metagenome and genome mining","volume":"174","author":"Siles","year":"2022","journal-title":"Soil Biol. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"106522","DOI":"10.1016\/j.envint.2021.106522","article-title":"Arsenic and antimony co-contamination influences on soil microbial community composition and functions: Relevance to arsenic resistance and carbon, nitrogen, and sulfur cycling","volume":"153","author":"Li","year":"2021","journal-title":"Environ. Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1128\/AEM.02919-14","article-title":"Geochemical Niches of Iron-Oxidizing Acidophiles in Acidic Coal Mine Drainage","volume":"81","author":"Jones","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"122336","DOI":"10.1016\/j.watres.2024.122336","article-title":"Enrichment of dissolved metal(loid)s and microbial organic matter during transit of a historic mine drainage system","volume":"266","author":"Sanchez","year":"2024","journal-title":"Water Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"108232","DOI":"10.1016\/j.soilbio.2021.108232","article-title":"Soil bacterial community functions and distribution after mining disturbance","volume":"157","author":"Xiao","year":"2021","journal-title":"Soil Biol. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4517","DOI":"10.1007\/s00203-021-02448-7","article-title":"Devosia sediminis sp. nov., isolated from subterranean sediment","volume":"203","author":"Ma","year":"2021","journal-title":"Arch. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1590\/S0103-84782005000600013","article-title":"Crescimento de Acidovorax avenae subsp. citrulli sob diferentes temperaturas, pH, concentra\u00e7\u00f5es de cloreto de s\u00f3dio e fontes de carbono","volume":"35","author":"Cavalcanti","year":"2005","journal-title":"Ci\u00eanc. Rural"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1099\/ijsem.0.002760","article-title":"Tabrizicola fusiformis sp nov., isolated from an industrial wastewater treatment plant","volume":"68","author":"Ko","year":"2018","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0168-1605(91)90063-U","article-title":"Influence of pH on the growth of some toxigenic species of Aspergillus, Penicillium and Fusarium","volume":"12","author":"Wheeler","year":"1991","journal-title":"Int. J. Food Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1111\/cla.12365","article-title":"Penicillium section Lanata-divaricata from acidic soil","volume":"35","author":"Diao","year":"2019","journal-title":"Cladistics"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.copbio.2016.01.013","article-title":"Microbial communities, processes and functions in acid mine drainage ecosystems","volume":"38","author":"Chen","year":"2016","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1007\/s10230-023-00945-3","article-title":"Silicified Microorganisms and Microorganism-Like Particles in the Groundwater of an Abandoned Coal Mine","volume":"42","author":"Shao","year":"2023","journal-title":"Mine Water Environ."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"133504","DOI":"10.1016\/j.jhazmat.2024.133504","article-title":"Role of indigenous microbial communities in the mobilization of potentially toxic elements and rare-earth elements from alkaline mine waste","volume":"466","author":"Cebekhulu","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Kinnunen, P., Miettinen, H., and Bomberg, M. (2020). Review of Potential Microbial Effects on Flotation. Minerals, 10.","DOI":"10.3390\/min10060533"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"103563","DOI":"10.1016\/j.earscirev.2021.103563","article-title":"From exploration to remediation: A microbial perspective for innovation in mining","volume":"216","author":"Levett","year":"2021","journal-title":"Earth-Sci. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zafeiropoulos, H., Paragkamian, S., Ninidakis, S., Pavlopoulos, G.A., Jensen, L.J., and Pafilis, E. (2022). PREGO: A Literature and Data-Mining Resource to Associate Microorganisms, Biological Processes, and Environment Types. Microorganisms, 10.","DOI":"10.3390\/microorganisms10020293"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"142521","DOI":"10.1016\/j.scitotenv.2020.142521","article-title":"Microbial communities in rare earth mining soil after in-situ leaching mining","volume":"755","author":"Liu","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Chen, D., and Feng, Q.Y. (2023). Microbial Community Structure, Metabolic Function, and Phenotypic Characteristics of Sediment in Deep Coal Mine Underground Environment, China. Water, 15.","DOI":"10.3390\/w15132371"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Maltman, C., Messner, K., Kyndt, J.A., and Yurkov, V. (2024). Brevundimonas aurifodinae, sp. nov., an Aerobic Anoxygenic Phototroph Resistant to Metalloid Oxyanions Isolated from Gold Mine Tailings. Microorganisms, 12, Erratum in Microorganisms\u00a02026, 14, 86. https:\/\/doi.org\/10.3390\/microorganisms14010086.","DOI":"10.3390\/microorganisms14010086"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.resmic.2014.07.007","article-title":"Microorganisms in subterranean acidic waters within Europe\u2019s deepest metal mine","volume":"165","author":"Kay","year":"2014","journal-title":"Res. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"131108","DOI":"10.1016\/j.chemosphere.2021.131108","article-title":"Bioleaching for environmental remediation of toxic metals and metalloids: A review on soils, sediments, and mine tailings","volume":"282","author":"Nguyen","year":"2021","journal-title":"Chemosphere"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Zhu, Z.K., Gao, Y.T., Zhang, L., and Sun, Y.J. (2025). Driving Effects of Coal Mining Activities on Microbial Communities and Hydrochemical Characteristics in Different Zones. Sustainability, 17.","DOI":"10.3390\/su17094000"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/2041\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T08:36:06Z","timestamp":1789374966000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/9\/2041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,13]]},"references-count":47,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["microorganisms14092041"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14092041","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,13]]}}}