{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T09:04:28Z","timestamp":1789635868134,"version":"4.0.0"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["22-14-00124-\u041f"],"award-info":[{"award-number":["22-14-00124-\u041f"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["25-44-20028"],"award-info":[{"award-number":["25-44-20028"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"award":["22-14-00124-\u041f"],"award-info":[{"award-number":["22-14-00124-\u041f"]}],"id":[{"id":"https:\/\/ror.org\/03y2gwe85","id-type":"ROR","asserted-by":"publisher"}]},{"award":["25-44-20028"],"award-info":[{"award-number":["25-44-20028"]}],"id":[{"id":"https:\/\/ror.org\/03y2gwe85","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The bioluminescence of entomopathogenic bacteria of the genus Photorhabdus was considered to be constitutive. However, it has recently been shown that under heat shock conditions, lux-operon is activated in Photorhabdus temperata cells. In the present study, bioluminescence thermoactivation was investigated in two isolated strains, Photorhabdus hainanensis FV2401 and P. temperata FV2402. The luminescence of the more thermotolerant P. hainanensis cells was activated at higher temperatures than that of P. temperata. At the maximum induction temperatures of 37 \u00b0C for FV2401 and 34 \u00b0C for FV2402, higher bioluminescence activation was observed in FV2401, whereas the increase in lux-genes mRNA levels was approximately the same in both strains. In P. hainanensis, elevated temperatures also affected the expression of the hexA, madB, and csrB genes, which are indirectly involved in luminescence in Photorhabdus. The heat-activated lux-operon promoter in P. hainanensis and P. temperata was determined. This is a \u03c370-dependent promoter that differs in its distance from the luxC start codon. When promoter variants with or without the 5\u2032-UTR mRNA were transferred to the heterologous Escherichia coli system, heat activation of luminescence was retained in constructs containing only the promoter and was independent of \u03c332 and \u03c3E, whereas constructs with the 5\u2032-UTR exhibited repression of luminescence following thermoactivation. The obtained data on the temperature-induced promoter of the lux-operon can broaden understanding of the ecological and biological functions of Photorhabdus bioluminescence under heat shock conditions and provide a basis for the development of novel biosensors.<\/jats:p>","DOI":"10.3390\/microorganisms14092076","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T08:03:17Z","timestamp":1789632197000},"page":"2076","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Thermally Induced lux-Operon Promoter of Photorhabdus hainanensis and Photorhabdus temperata"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5090-8787","authenticated-orcid":false,"given":"Vadim V.","family":"Fomin","sequence":"first","affiliation":[{"name":"Moscow Center for Advanced Studies, Moscow 123592, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"given":"Valeriia O.","family":"Matveeva","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, Moscow 123592, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3403-9395","authenticated-orcid":false,"given":"Kamilla V.","family":"Mekhantseva","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, Moscow 123592, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Daniil I.","family":"Sakharov","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, Moscow 123592, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6657-0418","authenticated-orcid":false,"given":"Sergei E.","family":"Spiridonov","sequence":"additional","affiliation":[{"name":"A.N. Severtsov Institute of Ecology and Evolution, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey V.","family":"Bazhenov","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, Moscow 123592, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2645-1120","authenticated-orcid":false,"given":"Nanette Hope","family":"Sumaya","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Iligan Institute of Technology, College of Science and Mathematics, Mindanao State University, Iligan City 9200, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilya V.","family":"Manukhov","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, Moscow 123592, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1099\/00207713-49-4-1645","article-title":"Polyphasic Classification of the Genus Photorhabdus and Proposal of New Taxa: P. luminescens Subsp. luminescens Subsp. 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