{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:03:39Z","timestamp":1789643019619,"version":"4.0.0"},"reference-count":49,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Key Laboratory of Veterinary Etiological Biology","award":["202449CE340019"],"award-info":[{"award-number":["202449CE340019"]}]},{"DOI":"10.13039\/501100011002","name":"NSFC-Yunnan Joint Fund","doi-asserted-by":"publisher","award":["U2202201"],"award-info":[{"award-number":["U2202201"]}],"id":[{"id":"10.13039\/501100011002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yunnan Fundamental Research Projects","award":["202401AT070440"],"award-info":[{"award-number":["202401AT070440"]}]},{"name":"Yunnan Fundamental Research Projects","award":["202601AT070152"],"award-info":[{"award-number":["202601AT070152"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Background: Although Toxoplasma gondii has been recognized as an obligate intracellular parasite, the lipid metabolic alterations it induces in colonic contents during acute infection remain poorly characterized. Methods: An acute infection model was established using C57BL\/6J mice by oral inoculation with the T. gondii ME49 strain. Untargeted lipidomics analysis was performed on the colonic contents collected at day 10 post-infection. Results: Acute infection significantly elevated glycerophospholipids (GPs) and reduced glycerolipids (GLs), and prenol lipids (PRs), leading to 136 differentially abundant lipids. Pathway enrichment analysis identified the two most significantly affected pathways: choline metabolism in cancer and glycerophospholipid metabolism, with lysophosphatidic acid (LPA(16:0)) and lysophosphatidylcholine (LPC(18:1)) functioning as core hub nodes. Receiver operating characteristic (ROC) analysis identified phosphatidylethanolamine (PE(20:1e_22:4)), PE(16:0_22:6), hexosylceramide (Hex1Cer(m18:0_20:4)), and Hex1Cer(t17:0_22:6) as exploratory candidate biomarkers with high discriminatory performance (AUC &gt; 0.94). Conclusions: Acute T. gondii infection induces lipid metabolic reprogramming in colonic contents of C57BL\/6J mice. The findings provide a novel metabolic perspective on the intestinal pathogenesis of toxoplasmosis and offer exploratory candidate lipid markers for diagnosing acute infection.<\/jats:p>","DOI":"10.3390\/microorganisms14092077","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T09:59:03Z","timestamp":1789639143000},"page":"2077","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Untargeted Lipidomics Reveals Lipid Alterations in Colonic Contents of C57BL\/6J Mice with Acute Toxoplasma gondii Infection"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9961-821X","authenticated-orcid":false,"given":"Wen-Jie","family":"Cheng","sequence":"first","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"First People\u2019s Hospital of Kunming City, Kunming 650032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo-Fang","family":"Duan","sequence":"additional","affiliation":[{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Yunnan Center for Animal Disease Prevention and Control, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai-Qin","family":"Deng","sequence":"additional","affiliation":[{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Animal Research and Resource Center, School of Life Sciences, Yunnan University, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng-Hao","family":"Wei","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Min","family":"Sui","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9212-3508","authenticated-orcid":false,"given":"Yi-Dan","family":"Wang","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2530-4628","authenticated-orcid":false,"given":"Xing-Quan","family":"Zhu","sequence":"additional","affiliation":[{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Shanxi Key Laboratory of Animal Disease Research, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9613-3734","authenticated-orcid":false,"given":"Feng-Cai","family":"Zou","sequence":"additional","affiliation":[{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7848-0152","authenticated-orcid":false,"given":"Zhao","family":"Li","sequence":"additional","affiliation":[{"name":"Animal Research and Resource Center, School of Life Sciences, Yunnan University, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/s13071-024-06141-8","article-title":"Anti-Toxoplasma gondii antibodies as a risk factor for the prevalence and severity of systemic lupus erythematosus","volume":"17","author":"Li","year":"2024","journal-title":"Parasit. 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