{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:02:53Z","timestamp":1790053373713,"version":"4.0.1"},"reference-count":24,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:00:00Z","timestamp":1789776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32560008"],"award-info":[{"award-number":["32560008"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Natural Science Foundation of China","award":["32360009"],"award-info":[{"award-number":["32360009"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"award":["32560008"],"award-info":[{"award-number":["32560008"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["32360009"],"award-info":[{"award-number":["32360009"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Tamarix Shrub Sand Dunes of the Tarim Basin","award":["525307007"],"award-info":[{"award-number":["525307007"]}]},{"name":"Tarim University Bochum Program","award":["BTYJM-2024-K30"],"award-info":[{"award-number":["BTYJM-2024-K30"]}],"id":[{"id":"https:\/\/ror.org\/05202v862","id-type":"ROR","asserted-by":"crossref"}]},{"award":["BTYJM-2024-K30"],"award-info":[{"award-number":["BTYJM-2024-K30"]}],"id":[{"id":"https:\/\/ror.org\/05202v862","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Talent Program of TianChi","award":["525307007"],"award-info":[{"award-number":["525307007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The genus Amycolatopsis, belonging to the phylum Actinobacteria, is known for producing diverse bioactive secondary metabolites and plays a significant role in microbial drug discovery. This study centers on the strain TRM77291, sourced from a unique habitat, and is postulated to represent a potential new species. This hypothesis is based on 16S rRNA gene phylogenetic analysis combined with physiological, biochemical, and chemotaxonomic characterization. The strain\u2019s genome, consisting of 8.76 Mb with a G + C content of 68.89% and encoding 8572 genes, was analyzed through whole-genome sequencing. AntiSMASH analysis identified 38 biosynthetic gene clusters related to PKS, NRPS, and RiPPs, while BIG-SCAPE analysis indicated the potential for unique compound synthesis, including Cluster 7 and Cluster 35. Screening ten fermentation media determined millet medium as the most optimal choice. Siderochelin A, a monomeric compound isolated from a fermentation product, demonstrated antibacterial efficacy against various pathogenic bacteria. This study revealed a notable activity of Siderochelin A against Escherichia coli, providing a theoretical basis for discovering novel species, exploring secondary metabolites, and developing innovative antibiotics.<\/jats:p>","DOI":"10.3390\/microorganisms14092096","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:25:43Z","timestamp":1789986343000},"page":"2096","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Whole-Genome Analysis, Biosynthetic Gene Cluster Mining of Amycolatopsis sp. nov. TRM77291 and Antibacterial Activity of Siderochelin A"],"prefix":"10.3390","volume":"14","author":[{"given":"Yi-Huang","family":"Chen","sequence":"first","affiliation":[{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu-Yao","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Xiang","family":"Yao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Ming","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhu-Feng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Xia","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1099\/ijs.0.000026-0","article-title":"Amycolatopsis marina sp. nov., an actinomycete isolated from an ocean sediment","volume":"59","author":"Bian","year":"2009","journal-title":"Int. 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