{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:07:06Z","timestamp":1792789626398,"version":"4.5.0"},"reference-count":62,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:00:00Z","timestamp":1789776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["42207150"],"award-info":[{"award-number":["42207150"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Natural Science Foundation of China","award":["32070112"],"award-info":[{"award-number":["32070112"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"award":["42207150"],"award-info":[{"award-number":["42207150"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["32070112"],"award-info":[{"award-number":["32070112"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012905","name":"Shandong Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2022QC179"],"award-info":[{"award-number":["ZR2022QC179"]}],"id":[{"id":"10.13039\/100012905","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012905","name":"Subject of Key R & D Plan of Shandong Province","doi-asserted-by":"publisher","award":["2024LZGC0030101"],"award-info":[{"award-number":["2024LZGC0030101"]}],"id":[{"id":"10.13039\/100012905","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Although hydrocarbon-degrading bacteria play a critical role in petroleum bioremediation, the substrate-specific metabolic pathways and adaptive mechanisms that underlie their competitive dominance remain poorly understood. Dietzia sp. CN-3, a salt-tolerant bacterium capable of utilizing both linear and branched alkanes, is an ideal model for investigating these regulatory networks. Here, we performed data-independent acquisition (DIA)-based quantitative proteomics to compare the proteomic landscapes of strain CN-3 grown on n-hexadecane (C16), pristane, and glucose. During growth on C16, strain CN-3 expressed a proposed terminal and subterminal oxidation pathway converting n-alkanes to acyl-CoA derivatives, involving AlkB and CYP153 hydroxylases, alcohol\/aldehyde dehydrogenases, Baeyer\u2013Villiger monooxygenases, and esterases. In contrast, pristane induced an alternative terminal oxidation pathway along with a substantially expanded repertoire of fatty acid \u03b2-oxidation enzymes to overcome steric hindrance. Functional heterologous expression of alkB in Pseudomonas fluorescens KOB2\u03941 restored growth on C12\u2013C16\u00a0n-alkanes and enhanced the growth of the pCom8-alkB recombinant on C28 (\u03bcmax = 0.065 d\u22121, ODmax = 0.333), confirming its role in medium- to long-chain alkane utilization. Our findings provide a systematic proteomic framework for understanding alkane oxidation in Dietzia, and offer mechanistic insights into the metabolic strategies that potentially drive strain CN-3\u2019s adaptability in hydrocarbon-contaminated environments.<\/jats:p>","DOI":"10.3390\/microorganisms14092100","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:25:43Z","timestamp":1789986343000},"page":"2100","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantitative Proteomics Reveals Substrate-Specific Metabolic Adaptations for n-Alkane and Branched Alkane Degradation in Dietzia sp. CN-3"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3923-2971","authenticated-orcid":false,"given":"Weiwei","family":"Chen","sequence":"first","affiliation":[{"name":"School of Life Sciences, Ludong University, Yantai 264025, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Ludong University, Yantai 264025, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Nian","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Ludong University, Yantai 264025, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Jun","family":"Min","sequence":"additional","affiliation":[{"name":"Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Shiwei","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Ludong University, Yantai 264025, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"137690","DOI":"10.1016\/j.jhazmat.2025.137690","article-title":"Development of a device to trap soil bacteria capable of degrading organic contaminants such as alkanes and polycyclic aromatic hydrocarbons","volume":"491","author":"Vauloup","year":"2025","journal-title":"J. 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