{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:00:49Z","timestamp":1790834449765,"version":"4.1.0"},"reference-count":34,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T00:00:00Z","timestamp":1543190400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFD0502200"],"award-info":[{"award-number":["2017YFD0502200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31672613, 31472242"],"award-info":[{"award-number":["31672613, 31472242"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010042","name":"Special Fund for Agro-scientific Research in the Public Interest","doi-asserted-by":"publisher","award":["201403054"],"award-info":[{"award-number":["201403054"]}],"id":[{"id":"10.13039\/501100010042","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Salmonella can cause enteric diseases in humans and a wide range of animals, and even outbreaks of foodborne illness. The aim of this study was to investigate the frequency and distribution of serovars, and antimicrobial resistance in Salmonella isolates from pigs with diarrhea in 26 provinces in China from 2014 to 2016. A total of 104 Salmonella isolates were identified and the dominant serovar was S. 4,[5],12:i:- (53.9%). All Salmonella isolates were resistant to trimethoprim-sulfamethoxazole, and many were resistant to ampicillin (80.8%) and tetracycline (76.9%). Among 104 Salmonella isolates, aac(6\u2032)-Ib-cr was the dominant plasmid-mediated quinolone resistance gene (80.8%), followed by qnrS (47.1%). The pulsed-field gel electrophoresis results suggest that the Salmonella isolates from different regions were genetically diverse, and ST34 was the most prevalent. S. 4,[5],12:i:- isolates is the widespread presence of heavy metal tolerance genes. The fact that the same sequence types were found in different regions and the high similarity coefficient of S. 4,[5],12:i:- isolates from different regions indicate the clonal expansion of the isolates, and the isolates carried various antimicrobial resistance genes. The multidrug resistant Salmonella can be widely detected in pigs, which will present a challenge for farm husbandry.<\/jats:p>","DOI":"10.3390\/microorganisms6040117","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T03:24:27Z","timestamp":1543202667000},"page":"117","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Distribution and Antimicrobial Resistance of Salmonella Isolated from Pigs with Diarrhea in China"],"prefix":"10.3390","volume":"6","author":[{"given":"Jin-Hui","family":"Su","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao-Hong","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian-Yi","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gui-Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian-Guo","family":"Jiao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiu-Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,11,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1146\/annurev-animal-022516-022834","article-title":"Salmonella in swine: Microbiota interactions","volume":"5","author":"Kim","year":"2017","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2040","DOI":"10.4315\/0362-028X.JFP-13-224","article-title":"Prevalence of extended-spectrum b-lactamase-producing Salmonella on retail chicken in six provinces and two national cities in the People\u2019s Republic of China","volume":"76","author":"Wu","year":"2013","journal-title":"J. Food Protect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.vetmic.2005.10.034","article-title":"Distribution of Salmonella enterica serovars from humans, livestock and meat in vietnam and the dominance of Salmonella typhimurium phage type 90","volume":"113","author":"Vo","year":"2006","journal-title":"Vet. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1051\/vetres:2008043","article-title":"Early immune response following Salmonella enterica subspecies enterica serovar Typhimurium infection in porcine jejunal gut loops","volume":"40","author":"Meurens","year":"2009","journal-title":"Vet. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"99","DOI":"10.4103\/0255-0857.124356","article-title":"Prevalence of Salmonella in pigs and broilers in the tarai region of uttarakhand, India","volume":"32","author":"Kumar","year":"2014","journal-title":"Indian J. Med. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1093\/jac\/dkp361","article-title":"Emergence of extended-spectrum beta-lactamases and ampc-type beta-lactamases in human Salmonella isolated in Spain from 2001 to 2005","volume":"64","author":"Arroyo","year":"2009","journal-title":"J. Antimicrob. Chemoth."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1093\/jac\/dkt209","article-title":"Spread of oqxab in Salmonella enterica serotype Typhimurium predominantly by IncHI2 plasmids","volume":"68","author":"Li","year":"2013","journal-title":"J. Antimicrob. Chemoth."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"521","DOI":"10.3389\/fmicb.2014.00521","article-title":"PMQR genes oqxAB and aac(6\u2032)Ib-cr accelerate the development of fluoroquinolone resistance in Salmonella Typhimurium","volume":"5","author":"Wong","year":"2014","journal-title":"Front. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1473-3099(15)00424-7","article-title":"Emergence of plasmid-mediated colistin resistance mechanism mcr-1 in animals and human beings in China: A microbiological and molecular biological study","volume":"16","author":"Liu","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.ijantimicag.2015.06.005","article-title":"Use of colistin-containing products within the European Union and European Economic Area (EU\/EEA): Development of resistance in animals and possible impact on human and animal health","volume":"46","author":"Catry","year":"2015","journal-title":"Int J. Antimicrob. Ag."},{"key":"ref_11","first-page":"477","article-title":"Prevalence of Salmonella in chicken carcasses in portugal","volume":"69","author":"Machado","year":"1990","journal-title":"J. Appl. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"715","DOI":"10.3389\/fmicb.2018.00715","article-title":"A comparative genomic analysis provides novel insights into the ecological success of the monophasic Salmonella serovar 4,[5],12:I","volume":"9","author":"Mastrorilli","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"617","DOI":"10.3201\/eid2204.150531","article-title":"Microevolution of monophasic Salmonella Typhimurium during epidemic, United Kingdom, 2005\u20132010","volume":"22","author":"Petrovska","year":"2016","journal-title":"Emerg Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1111\/j.1863-2378.2012.01487.x","article-title":"Application of the random forest method to analyse epidemiological and phenotypic characteristics of Salmonella 4,[5],12:I:- and Salmonella Typhimurium strains","volume":"59","author":"Barco","year":"2012","journal-title":"Zoonoses Public Health"},{"key":"ref_15","unstructured":"Clinical and Laboratory Standards Institute (2015). Performance Standards for Antimicrobial Susceptibility Testing. Twenty-fifth Informational Supplement, Clinical and laboratory standards institute. M100-s25."},{"key":"ref_16","unstructured":"Clinical and Laboratory Standards Institute (2013). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals, Clinical and laboratory standards institute. [4th ed.]."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/AEM.70.1.1-7.2004","article-title":"Characterization of multiple-antimicrobial-resistant Salmonella serovars isolated from retail meats","volume":"70","author":"Chen","year":"2004","journal-title":"Appl. Environ. Microb."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1128\/AAC.47.2.798-799.2003","article-title":"Conjugative plasmid conferring resistance to olaquindox","volume":"47","author":"Sorensen","year":"2003","journal-title":"Antimicrob. Agents Ch."},{"key":"ref_19","unstructured":"European Committee on Antimicrobial Susceptibility Testing (2017). Breakpoint tables for interpretation of mics and zone diameters version 7.1, valid from 2017-03-10."},{"key":"ref_20","unstructured":"Centers for Disease Control Prevention (2013). Standard Operating Procedure for Pulsenet Pfge of Escherichia coli o157: H7, Escherichia coli non-o157 (stec), Salmonella serotypes, Shigella sonnei and Shigella flexneri."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1038\/cr.2010.45","article-title":"Rck of Salmonella enterica, subspecies enterica serovar enteritidis, mediates zipper-like internalization","volume":"20","author":"Rosselin","year":"2010","journal-title":"Cell. Res."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Kaas, R.S., Leekitcharoenphon, P., Aarestrup, F.M., and Lund, O. (2014). Solving the problem of comparing whole bacterial genomes across different sequencing platforms. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0104984"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.ijfoodmicro.2014.03.030","article-title":"Serotype distribution and antibiotic resistance of Salmonella in food-producing animals in Shandong province of China, 2009 and 2012","volume":"180","author":"Lai","year":"2014","journal-title":"Int. J. Food Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"602","DOI":"10.3389\/fmicb.2015.00602","article-title":"Serotypes and antimicrobial susceptibility of Salmonella spp. Isolated from farm animals in China","volume":"6","author":"Kuang","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.ijfoodmicro.2013.01.020","article-title":"Prevalence and characterization of Salmonella species isolated from pigs, ducks and chickens in Sichuan province, China","volume":"163","author":"Li","year":"2013","journal-title":"Int. J. Food. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (2015). The European union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013. EFSA J., 18, 45.","DOI":"10.2903\/j.efsa.2015.3991"},{"key":"ref_27","unstructured":"National Antimicrobial Resistance Monitoring System (2018, November 18). Animal Arm Annual Report, Available online: Https:\/\/www.Ars.Usda.Gov\/arsuserfiles\/60401020\/narms\/narms2011\/narms%20usda%202011%20report.Pdf."},{"key":"ref_28","unstructured":"National Bureau of Statistics of China (2016). China Statistical Yearbook."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2300","DOI":"10.1093\/jac\/dkw093","article-title":"Detection of the plasmid-mediated mcr-1 gene conferring colistin resistance in human and food isolates of Salmonella enterica and Escherichia coli in England and Wales","volume":"71","author":"Doumith","year":"2016","journal-title":"J. Antimicrob. Chemoth."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1080\/03079457.2013.788129","article-title":"Antimicrobial resistance, presence of integrons and biofilm formation of Salmonella pullorum isolates from eastern China (1962-2010)","volume":"42","author":"Gong","year":"2013","journal-title":"Avian Pathol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1017\/S1751731111000188","article-title":"Effect of rearing environment and dietary zinc oxide on the response of group-housed weaned pigs to enterotoxigenic Escherichia coli O149 challenge","volume":"5","author":"Slade","year":"2011","journal-title":"Animal"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"567","DOI":"10.3390\/antibiotics4040567","article-title":"Co-selection of resistance to antibiotics, biocides and heavy metals, and its relevance to foodborne pathogens","volume":"4","author":"Wales","year":"2015","journal-title":"Antibiotics"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1089\/fpd.2011.1008","article-title":"Laboratory-based surveillance of non-typhoidal Salmonella infections in Guangdong province, China","volume":"9","author":"Deng","year":"2012","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1089\/fpd.2012.1154","article-title":"Occurrence and characterization of monophasic Salmonella enterica serovar Typhimurium (4,[5], 12:i:-) of non-human origin in Poland","volume":"9","author":"Wasyl","year":"2012","journal-title":"Foodborne Pathog. Dis."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/6\/4\/117\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:32:00Z","timestamp":1760196720000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/6\/4\/117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,26]]},"references-count":34,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["microorganisms6040117"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms6040117","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,26]]}}}