{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T08:42:30Z","timestamp":1791448950619,"version":"4.3.3"},"reference-count":39,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T00:00:00Z","timestamp":1557360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Burkholderia pseudomallei, the causative agent of melioidosis can occur in healthy humans, yet binge alcohol use is progressively being recognized as a major risk factor. Currently, no experimental studies have investigated the effects of binge alcohol on the adaptive immune system during an active infection. In this study, we used B. thailandensis and B. vietnamiensis, to investigate the impact of a single binge alcohol episode on the humoral response during infection. Eight-week-old female C57BL\/6 mice were administered alcohol comparable to human binge drinking (4.4 g\/kg) or PBS intraperitoneally 30 min before intranasal infection. Mice infected with B. thailandensis had a 100% survival rate, while those infected with B. vietnamiensis had a 33% survivability rate when a binge alcohol dose was administered. B. thailandensis was detected in blood of mice administered alcohol at only 7 days post infection (PI), while those infected with B. vietnamiensis and receiving alcohol were found throughout the 28-day infection as well as in tissues at day 28 PI. Binge alcohol elevated IgM and delayed IgG specific to the whole cell lysate (WCL) of B. vietnamiensis but not B. thailandensis infections. Differences in immunogenicity of B. pseudomallei near-neighbors provide a framework for novel insights into the effects of binge alcohol\u2019s suppression of the humoral immune response that can cause opportunistic infections in otherwise healthy hosts.<\/jats:p>","DOI":"10.3390\/microorganisms7050125","type":"journal-article","created":{"date-parts":[[2019,5,13]],"date-time":"2019-05-13T03:57:07Z","timestamp":1557719827000},"page":"125","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Impact of Binge Alcohol Intoxication on the Humoral Immune Response during Burkholderia spp. Infections"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2119-9413","authenticated-orcid":false,"given":"Ryan M.","family":"Moreno","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Victor","family":"Jimenez","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA"},{"name":"Pathogen &amp; Microbiome Institute (PMI), Northern Arizona University, Flagstaff, AZ 86011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3466-7466","authenticated-orcid":false,"given":"Fernando P.","family":"Monroy","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA"},{"name":"Pathogen &amp; Microbiome Institute (PMI), Northern Arizona University, Flagstaff, AZ 86011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nmicrobiol.2015.8","article-title":"Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis","volume":"1","author":"Limmathurotsakul","year":"2016","journal-title":"Nat. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1086\/318116","article-title":"Endemic melioidosis in tropical northern Australia: A 10-Year prospective study and review of the literature","volume":"31","author":"Currie","year":"2000","journal-title":"Clin. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1055\/s-0034-1398389","article-title":"Melioidosis: Evolving concepts in epidemiology, pathogenesis, and treatment","volume":"36","author":"Currie","year":"2015","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1111\/j.1365-3156.2004.01328.x","article-title":"Melioidosis epidemiology and risk factors from a prospective whole-population study in northern Australia","volume":"9","author":"Currie","year":"2004","journal-title":"Trop. Med. Int. Health"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e900","DOI":"10.1371\/journal.pntd.0000900","article-title":"The epidemiology and clinical spectrum of melioidosis: 540 cases from the 20 year darwin prospective study","volume":"4","author":"Currie","year":"2010","journal-title":"PLoS Negl. Trop. Dis."},{"key":"ref_6","first-page":"177","article-title":"Alcohol use as a risk factor in infections and healing: A clinician\u2019s perspective","volume":"37","author":"Kolls","year":"2015","journal-title":"Alcohol Res. Curr. Rev."},{"key":"ref_7","unstructured":"National Institutes of Alcohol Abuse and Alcoholism (2019, March 01). Drinking Levels Defined, Available online: https:\/\/www.niaaa.nih.gov\/alcohol-health\/overview-alcohol-consumption\/moderate-binge-drinking."},{"key":"ref_8","first-page":"199","article-title":"Alcohol\u2019s effects on lung health and immunity","volume":"37","author":"Simet","year":"2015","journal-title":"Alcohol Res."},{"key":"ref_9","first-page":"243","article-title":"Alcohol and the lung","volume":"38","author":"Mehta","year":"2017","journal-title":"Alcohol Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.alcohol.2017.04.004","article-title":"Effects of binge alcohol exposure on Burkholderia thailandensis\u2013alveolar macrophage interaction","volume":"64","author":"Jimenez","year":"2017","journal-title":"Alcohol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1086\/323661","article-title":"Acute ethanol intoxication suppresses lung chemokine production following Infection with Streptococcus pneumoniae","volume":"184","author":"Nelson","year":"2001","journal-title":"J. Infect. Dis."},{"key":"ref_12","first-page":"159","article-title":"Alcohol\u2032s effect on host defense","volume":"37","author":"Szabo","year":"2015","journal-title":"Alcohol Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1513\/pats.200507-065JS","article-title":"Alcohol, immunosuppression, and the lung","volume":"2","author":"Happel","year":"2005","journal-title":"Proc. Am. Thorac. Soc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1111\/j.1530-0277.1995.tb01548.x","article-title":"Modulation of T-cell adhesion markers, and the CD45R and CD57 antigens in human alcoholics","volume":"19","author":"Cook","year":"1995","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1111\/j.1600-065X.2010.00941.x","article-title":"Roles of B-1 and B-2 cells in innate and acquired IgA-mediated immunity","volume":"237","author":"Suzuki","year":"2010","journal-title":"Immunol. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5402","DOI":"10.1128\/IAI.00626-08","article-title":"Burkholderia thailandensis as a model system for the study of the virulence-associated type III secretion system of Burkholderia pseudomallei","volume":"76","author":"Haraga","year":"2008","journal-title":"Infect. Immun."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e02802","DOI":"10.1128\/AEM.02802-16","article-title":"Burkholderia humptydooensis sp. nov., A new species related to Burkholderia thailandensis and the fifth member of the Burkholderia pseudomallei Complex","volume":"83","author":"Tuanyok","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0208061","article-title":"A mouse model of binge alcohol consumption and Burkholderia infection","volume":"13","author":"Jimenez","year":"2018","journal-title":"PLoS ONE"},{"key":"ref_19","first-page":"1","article-title":"Development of capsular polysaccharide-based glycoconjugates for immunization against melioidosis and glanders","volume":"2","author":"Burtnick","year":"2012","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_20","first-page":"3916","article-title":"Purification and visualization of lipopolysaccharide from Gram-negative bacteria by hot aqueous-phenol extraction","volume":"63","author":"Davis","year":"2012","journal-title":"J. Vis. Exp. JoVE"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jim.2005.11.019","article-title":"A novel chemistry for conjugating pneumococcal polysaccharides to Luminex microspheres","volume":"309","author":"Schlottmann","year":"2006","journal-title":"J. Immunol. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1186\/1475-2875-13-410","article-title":"Comparative analysis of IgG responses to Plasmodium falciparum MSP1p19 and PF13-DBL1\u03b11 using ELISA and a magnetic bead-based duplex assay (MAGPIX\u00ae-Luminex) in a Senegalese meso-endemic community","volume":"13","author":"Perraut","year":"2014","journal-title":"Malar. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/gb-2010-11-8-r89","article-title":"Genomic acquisition of a capsular polysaccharide virulence cluster by nonpathogenic","volume":"11","author":"Sim","year":"2010","journal-title":"Burkholderia isolates. Genome Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0091682","article-title":"Variable virulence factors in Burkholderia pseudomallei (Melioidosis) associated with human disease","volume":"9","author":"Sarovich","year":"2014","journal-title":"PLoS ONE"},{"key":"ref_25","first-page":"1410","article-title":"Immune response to Burkholderia pseudomallei","volume":"94","author":"Panomket","year":"2011","journal-title":"J. Med. Assoc. Thail."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1128\/IAI.70.2.504-511.2002","article-title":"Model of differential susceptibility to mucosal Burkholderia pseudomallei Infection","volume":"70","author":"Liu","year":"2002","journal-title":"Infect. Immun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s00253-010-2528-0","article-title":"Pathogenicity, virulence factors, and strategies to fight against Burkholderia cepacia complex pathogens and related species","volume":"87","author":"Sousa","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1111\/j.1469-0691.2010.03237.x","article-title":"Burkholderia cenocepacia in cystic fibrosis: Epidemiology and molecular mechanisms of virulence","volume":"16","author":"Drevinek","year":"2010","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1086\/423141","article-title":"Production of exopolysaccharide by Burkholderia cenocepacia results in altered cell-surface interactions and altered bacterial clearance in mice","volume":"190","author":"Conway","year":"2004","journal-title":"J. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Hall, C.M., Busch, J.D., Shippy, K., Allender, C.J., Kaestli, M., Mayo, M., Sahl, J.W., Schupp, J.M., Colman, R.E., and Keim, P. (2015). Diverse Burkholderia species isolated from Soils in the Southern United States with no Evidence of B. pseudomallei. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0143254"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1099\/jmm.0.45766-0","article-title":"Evaluation of lipopolysaccharide and capsular polysaccharide as subunit vaccines against experimental melioidosis","volume":"53","author":"Nelson","year":"2004","journal-title":"J. Med. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Houghton, R.L., Reed, D.E., Hubbard, M.A., Dillon, M.J., Chen, H., Currie, B.J., Mayo, M., Sarovich, D.S., Theobald, V., and Limmathurotsakul, D. (2014). Development of a prototype lateral flow immunoassay (LFI) for the rapid diagnosis of melioidosis. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002727"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3206","DOI":"10.1128\/IAI.01847-14","article-title":"Burkholderia pseudomallei capsular polysaccharide conjugates provide protection against acute melioidosis","volume":"82","author":"Scott","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/j.vaccine.2014.11.057","article-title":"Protection of non-human primates against glanders with a gold nanoparticle glycoconjugate vaccine","volume":"33","author":"Torres","year":"2015","journal-title":"Vaccine"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1128\/JCM.02856-15","article-title":"Development of rapid Enzyme-Linked Immunosorbent Assays for detection of antibodies to Burkholderia pseudomallei","volume":"54","author":"Suttisunhakul","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_36","first-page":"185","article-title":"Impact of alcohol abuse on the adaptive immune system","volume":"37","author":"Pasala","year":"2015","journal-title":"Alcohol Res. Curr. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1080\/21505594.2016.1176655","article-title":"Contribution of murine IgG Fc regions to antibody binding to the capsule of Burkholderia pseudomallei","volume":"7","author":"Dillon","year":"2016","journal-title":"Virulence"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/BF02918616","article-title":"Alcohol can inhibit effect of IL-4 on activated murine B cells","volume":"11","author":"Pratt","year":"1992","journal-title":"Immun. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5370","DOI":"10.4049\/jimmunol.1401849","article-title":"Ig heavy chain class switch recombination: Mechanism and regulation","volume":"193","author":"Stavnezer","year":"2015","journal-title":"J. Immun."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/7\/5\/125\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:50:19Z","timestamp":1760187019000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/7\/5\/125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,9]]},"references-count":39,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2019,5]]}},"alternative-id":["microorganisms7050125"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms7050125","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,9]]}}}