{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T18:58:44Z","timestamp":1771009124028,"version":"3.50.1"},"reference-count":119,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T00:00:00Z","timestamp":1564531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Atma Jaya Catholic University of Indonesia, Surya University, Unversitas Indonesia and Universitas Nasional","award":["Research Funding Scheme 2018"],"award-info":[{"award-number":["Research Funding Scheme 2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The Zika virus disease, also known as Zika fever is an arboviral disease that became epidemic in the Pacific Islands and had spread to 18 territories of the Americas in 2016. Zika virus disease has been linked to several health problems such as microcephaly and the Guillain\u2013Barr\u00e9 syndrome, but to date, there has been no vaccine available for Zika. Problems related to the development of a vaccine include the vaccination target, which covers pregnant women and children, and the antibody dependent enhancement (ADE), which can be caused by non-neutralizing antibodies. The peptide vaccine was chosen as a focus of this study as a safer platform to develop the Zika vaccine. In this study, a collection of Zika proteomes was used to find the best candidates for T- and B-cell epitopes using the immunoinformatics approach. The most promising T-cell epitopes were mapped using the selected human leukocyte antigen (HLA) alleles, and further molecular docking and dynamics studies showed a good peptide-HLA interaction for the best major histocompatibility complex-II (MHC-II) epitope. The most promising B-cell epitopes include four linear peptides predicted to be cross-reactive with T-cells, and conformational epitopes from two proteins accessible by antibodies in their native biological assembly. It is believed that the use of immunoinformatics methods is a promising strategy against the Zika viral infection in designing an efficacious multiepitope vaccine.<\/jats:p>","DOI":"10.3390\/microorganisms7080226","type":"journal-article","created":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T11:37:07Z","timestamp":1564573027000},"page":"226","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Peptide-Based Subunit Vaccine Design of T- and B-Cells Multi-Epitopes against Zika Virus Using Immunoinformatics Approaches"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2992-1247","authenticated-orcid":false,"given":"Vivitri Dewi","family":"Prasasty","sequence":"first","affiliation":[{"name":"Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karel","family":"Grazzolie","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Life Science, Surya University, Tangerang, Banten 15143, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosmalena","family":"Rosmalena","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Depok 16424, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatmawaty","family":"Yazid","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Depok 16424, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fransiskus Xaverius","family":"Ivan","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Life Science, Surya University, Tangerang, Banten 15143, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernawati","family":"Sinaga","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Universitas Nasional, Jakarta 12520, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.tim.2016.05.002","article-title":"Zika Virus: Two or Three Lineages?","volume":"24","author":"Gong","year":"2016","journal-title":"Trends Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1007\/s12250-016-3774-9","article-title":"Phylogenetic analysis revealed the central roles of two African countries in the evolution and worldwide spread of Zika virus","volume":"31","author":"Shen","year":"2016","journal-title":"Virol. 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