{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:59:50Z","timestamp":1790614790902,"version":"4.1.0"},"reference-count":43,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,12,13]],"date-time":"2019-12-13T00:00:00Z","timestamp":1576195200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Rickettsiae have worldwide occurrence and rickettsiosis are widely recognized as emerging infections in several parts of the world. For decades, it was thought that a single pathogenic tick-borne spotted fever group (SFG), Rickettsia, occurred in each continent. Nowadays, thanks to molecular biology, new species of Rickettsia responsible for disease in humans are continuously identified worldwide. In a framework of diagnostic activities of the Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise \u201cG. Gaporale\u201d and considering some reports of suspected human clinical cases of rickettsiosis, a survey on ticks collected form animals and humans was carried out with the aim to identify the Rickettsia species circulating in Abruzzi and Molise regions. A total of 603 ticks, previously identified at species level by morphology, pooled into 178 tick samples, were tested by pan-Rickettsia RealTime PCR. DNA from specimens positive for Rickettsia spp. was then sequenced in order to identify the Rickettsia species involved. The highest infection rate was detected in Dermacentor marginatus followed by Ixodes ricinus. The selected targets for this purpose were OmpA and gltA. Rickettsia slovaca, Rickettsia monacensis, Rickettsia massiliae, Rickettsia conorii, Rickettsia aeschlimannii, Rickettsia helvetica, Rickettsia raoultii, and Rickettsia felis \u2013 like organisms were identified in this study. These are the first data available in the literature for the circulation of SFG Rickettsia species in the selected geographical area. Results made evidence of high rate of infection in ticks. All Rickettsia species detected have been previously involved in human infection. The diversity of Rickettsia detected, and tick species collected reflects the biodiversity in term of wildlife and environment of the area. An association between Rickettsia species, ticks, and the relationships with vertebrate host species are discussed. Due to the peculiar eco-biology of each Rickettsia species, the use of diagnostic tools able to identify Rickettsia at the species level is thus recommended in order to assess the risk for humans and to elucidate more precise etiological diagnosis in clinical cases.<\/jats:p>","DOI":"10.3390\/microorganisms7120696","type":"journal-article","created":{"date-parts":[[2019,12,13]],"date-time":"2019-12-13T11:27:22Z","timestamp":1576236442000},"page":"696","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Diversity of Rickettsia in Ticks Collected in Abruzzi and Molise Regions (Central Italy)"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0041-4599","authenticated-orcid":false,"given":"Ilaria","family":"Pascucci","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise \u201cG. Caporale\u201d, 64100 Teramo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4795-8323","authenticated-orcid":false,"given":"Marco","family":"Di Domenico","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise \u201cG. Caporale\u201d, 64100 Teramo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2335-3154","authenticated-orcid":false,"given":"Valentina","family":"Curini","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise \u201cG. Caporale\u201d, 64100 Teramo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Cocco","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise \u201cG. Caporale\u201d, 64100 Teramo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Averaimo","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise \u201cG. Caporale\u201d, 64100 Teramo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"D\u2019Alterio","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise \u201cG. Caporale\u201d, 64100 Teramo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7547-1195","authenticated-orcid":false,"given":"Cesare","family":"Camm\u00e0","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise \u201cG. Caporale\u201d, 64100 Teramo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.00032-13","article-title":"Update on tick borne rickettsioses around the world: A geographic approach","volume":"26","author":"Parola","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1128\/CMR.18.4.719-756.2005","article-title":"Tick-borne rickettsioses around the world: Emerging diseases challenging old concepts","volume":"18","author":"Parola","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1051\/vetres\/2009017","article-title":"The relationship between spotted fever group Rickettsiae and ixodid ticks","volume":"40","author":"Socolovschi","year":"2009","journal-title":"Vet. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/j.1469-0691.2008.02278.x","article-title":"Transovarial and trans-stadial transmission of Rickettsiae africae in Amblyomma variegatum ticks","volume":"15","author":"Socolovschi","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1089\/vbz.2005.5.363","article-title":"Experimental infection models of ticks of the Rhipicephalus sanguineus group with Rickettsia conorii","volume":"5","author":"Matsumoto","year":"2005","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/j.1749-6632.2003.tb07378.x","article-title":"Mediterranean spotted fever in Portugal: Risk factors for fatal outcome in 105 hospitalized patients","volume":"990","author":"Nobrega","year":"2003","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s10156-011-0295-1","article-title":"Mediterranean spotted fever and encephalitis: A case report and review of the literature","volume":"18","author":"Duque","year":"2012","journal-title":"J. Infect. Chemother."},{"key":"ref_8","first-page":"3","article-title":"Le rickettsiosi in Italia: Diagnosi e sorveglianza","volume":"28","author":"Mancini","year":"2015","journal-title":"Not. Ist. Super Sanit\u00e0"},{"key":"ref_9","unstructured":"Manilla, G. (1998). Acari Ixodida. Fauna d\u2019Italia, Edizioni Calderini. [1st ed.]."},{"key":"ref_10","unstructured":"Graziani, C., Duranti, A., Morelli, A., Busani, L., and Pezzoti, P. (2016). Zoonosi in Italia Nel Periodo 2009\u20132013, Istituto Superiore di Sanit\u00e0. Rapporti ISTISAN."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s10493-012-9535-z","article-title":"Etiological agents of rickettsiosis and anaplasmosis in ticks collected in Emilia-Romagna region (Italy) during 2008 and 2009","volume":"57","author":"Maioli","year":"2012","journal-title":"Exp. Appl. Acarol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1016\/j.ttbdis.2016.06.003","article-title":"Molecular detection and characterization of spotted fever group rickettsiae in ticks from Central Italy","volume":"7","author":"Scarpulla","year":"2016","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s10493-017-0132-z","article-title":"Rickettsia spp. in Dermacentor marginatus ticks: Analysis of the host\u2013vector\u2013pathogen interactions in a northern Mediterranean area","volume":"72","author":"Selmi","year":"2017","journal-title":"Exp. Appl. Acarol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.ttbdis.2016.12.007","article-title":"Detection of Rickettsia hoogstraalii, Rickettsia helvetica, Rickettsia massiliae, Rickettsia slovaca and Rickettsia aeschlimannii in ticks from Sardinia, Italy","volume":"8","author":"Chisu","year":"2017","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.ttbdis.2018.09.001","article-title":"Mediterranean spotted fever rickettsiosis in Italy, 2001\u20132015: Spatio-temporal distribution based on hospitalization records","volume":"10","author":"Vescio","year":"2019","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_16","unstructured":"Di Domenico, M., Camm\u00e0, C., Curini, V., Dall\u2019Acqua, F., Di Sabatino, D., and Pascucci, I. (2016, January 26\u201329). Molecular survey of tick-borne pathogens in wild boars from Central Italy. Proceedings of the 15th Biodefence Conference, Munich, Germany."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Pascucci, I., Di Domenico, M., Angelico, G., Curini, V., Camm\u00e0, C., and Sozio, G. (2016, January 2\u20137). Ecology of tick-borne pathogens: Investigation on rodents and ticks in protected areas in Central Italy. Proceedings of the 20th International Conference of European Society for Vector Ecology E Sove, Lisbon, Portugal.","DOI":"10.1016\/j.ttbdis.2016.03.012"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1128\/JCM.01723-12","article-title":"Assessment of real-time PCR assay for detection of Rickettsia spp. and Rickettsia rickettsii in banked clinical samples","volume":"51","author":"Kato","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.eimc.2012.08.001","article-title":"Usefulness of rickettsial PCR assays for the molecular diagnosis of human rickettsioses","volume":"31","author":"Portillo","year":"2013","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.apjtm.2015.07.033","article-title":"Molecular survey of tick-borne pathogens in Ixodid ticks collected from hunted wild animals in Tuscany, Italy","volume":"8","author":"Ebani","year":"2015","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_21","first-page":"143","article-title":"Detection of tick-borne pathogens in ticks collected in the suburban area of Monte Romano, Lazio Region, Central Italy","volume":"55","author":"Mancini","year":"2019","journal-title":"Ann. Ist Super Sanita"},{"key":"ref_22","first-page":"181","article-title":"Epidemiological consequences of host specificity of ticks (Ixodida)","volume":"58","author":"Kiewra","year":"2012","journal-title":"Ann. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1017\/S003118200001859X","article-title":"The host relationships of Ixodes hexagonus leach in Britain","volume":"43","author":"Arthur","year":"1953","journal-title":"Parasitology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s10493-016-0035-4","article-title":"Phylogeography of Rhipicephalus sanguineus sensu lato and its relationships with climatic factors","volume":"69","author":"Zemtsova","year":"2016","journal-title":"Exp. Appl. Acarol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s10493-018-0257-8","article-title":"Analysis of the environmental and host-related factors affecting the distribution of the tick Dermacentor marginatus","volume":"75","author":"Selmi","year":"2018","journal-title":"Exp. Appl. Acarol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3421","DOI":"10.1007\/s00436-018-6036-y","article-title":"First molecular detection of the human pathogen Rickettsia raoultii and other spotted fever group Rickettsiae in Ixodid ticks from wild and domestic mammals","volume":"117","author":"Chisu","year":"2018","journal-title":"Parasitol. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1099\/jmm.0.021543-0","article-title":"Pathogens and symbionts in ticks: A survey on tick species distribution and presence of tick-transmitted micro-organisms in Sardinia, Italy","volume":"60","author":"Satta","year":"2011","journal-title":"J. Med. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"702","DOI":"10.3201\/eid1804.111583","article-title":"Rickettsia monacensis as cause of Mediterranean spotted fever-like illness, Italy","volume":"18","author":"Madeddu","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_29","first-page":"188","article-title":"Population dynamics and management of Sus scrofa in western Switzerland: A statistical modeling approach","volume":"3","author":"Neet","year":"1995","journal-title":"Ibex JME"},{"key":"ref_30","unstructured":"Apollonio, M., Andersen, R., and Putman, R. (2010). European Ungulates and Their Management in the 21st Century, Cambridge University Press."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Socolovschi, C., Gaudart, J., Bitam, I., Huynh, T.P., Raoult, D., and Parola, P. (2012). Why are there so few Rickettsia conorii conorii-infected Rhipicephalus sanguineus ticks in the wild?. PLoS Negl. Trop. Dis., 6.","DOI":"10.1371\/journal.pntd.0001697"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1099\/ijs.0.64952-0","article-title":"Rickettsia raoultii sp. nov., a spotted fever group Rickettsia associated with Dermacentor ticks in Europe and Russia","volume":"58","author":"Mediannikov","year":"2008","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1089\/vbz.2013.1458","article-title":"Detection of Microbial Agents in Ticks Collected from Migratory Birds in Central Italy","volume":"14","author":"Toma","year":"2014","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_34","first-page":"318","article-title":"Spotted fever Rickettsia species in Hyalomma and Ixodes ticks infesting migratory birds in the European Mediterranean area","volume":"10","author":"Barboutis","year":"2014","journal-title":"Parasit. Vectors"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"101272","DOI":"10.1016\/j.ttbdis.2019.101272","article-title":"Assessing the role of migratory birds in the introduction of ticks and tick-borne pathogens from African countries: An Italian experience","volume":"10","author":"Pascucci","year":"2019","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1099\/00207713-47-2-548","article-title":"Rickettsia aeschlimannii sp. nov., a new spotted fever group rickettsia associated with Hyalomma marginatum ticks","volume":"47","author":"Beati","year":"1997","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_37","first-page":"2630","article-title":"Human Rickettsia aeschlimannii infection: First case with acute hepatitis and review of the literature","volume":"20","author":"Tosoni","year":"2016","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.pt.2016.04.009","article-title":"Rickettsia felis: The complex journey of an emergent human pathogen","volume":"32","author":"Angelakis","year":"2016","journal-title":"Trends Parasitol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1196\/annals.1374.068","article-title":"Molecular identification of Rickettsia felis-like bacteria in Haemaphysalis sulcata ticks collected from domestic animals in southern Croatia","volume":"1078","author":"Duh","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1099\/ijs.0.011049-0","article-title":"Rickettsia hoogstraalii sp. nov., isolated from hard- and soft-bodied ticks","volume":"60","author":"Duh","year":"2010","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Raele, D.A., Galante, D., Pugliese, N., Salandra, G., and Cafiero, M.A. (2018). Spotted fever group rickettsiae associated with ixodid ticks in wild environment in Southern Italy. Microbiologyopen, 7.","DOI":"10.1002\/mbo3.527"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1186\/s13071-018-2947-9","article-title":"Prevalence and diversity of Rickettsia species in ectoparasites collected from small rodents in Lithuania","volume":"28","author":"Radzijevskaja","year":"2018","journal-title":"Parasites Vectors"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.meegid.2014.03.014","article-title":"Genotyping, evolution and epidemiological findings of Rickettsia species","volume":"25","author":"Merhej","year":"2014","journal-title":"Infect. Genet. Evol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/7\/12\/696\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:42:16Z","timestamp":1760190136000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/7\/12\/696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,13]]},"references-count":43,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2019,12]]}},"alternative-id":["microorganisms7120696"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms7120696","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,13]]}}}