{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:57:16Z","timestamp":1791899836524,"version":"4.3.4"},"reference-count":20,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T00:00:00Z","timestamp":1582848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009553","name":"Czech Health Research Council","doi-asserted-by":"publisher","award":["17-29239A"],"award-info":[{"award-number":["17-29239A"]}],"id":[{"id":"10.13039\/501100009553","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Charles University Research Fund PROGRES","award":["Q39"],"award-info":[{"award-number":["Q39"]}]},{"name":"National Sustainability Program I (NPU I)","award":["LO1503"],"award-info":[{"award-number":["LO1503"]}]},{"name":"Ministry of Education Youth and Sports of the Czech Republic","award":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000787"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000787"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Background: The spread of carbapenemase genes, such as blaNDM-1, in Proteus mirabilis poses a public health threat. The aim of the study was to characterize the genome and plasmids sequences of an NDM-1-positive strain (IBCRE14), which was isolated in 2019 from a catheterized patient hospitalized in Italy. Methods: Whole genome sequencing (WGS) of IBCRE14 was performed on extracted genomic DNA using Sequel I platform. Genome assembly was performed using \u201cMicrobial Assembly\u201d. Genomic analysis was conducted by uploading the contigs to ResFinder and PlasmidFinder databases from the Center for Genomic Epidemiology. Results: IBCRE14 had a genome size of 4,018,329 bp and harboured genes coding for resistance to aminoglycosides (aadA1), phenicol (cat), tetracycline (tetJ), and trimethoprim (dfrA1). A large plasmid (pIB_NDM_1) harboured antibiotic resistance genes against sulphonamide (sul1), trimethoprim (dfrA14), tetracycline (tetB), rifampicin (arr-2), aminoglycosides (aadA1, aph3-VI), and beta-lactams (blaOXA-10, blaNDM-1). Furthermore, a small plasmid (pIB_COL3M) harboured a qnrD1 gene coding for quinolone resistance. Conclusion: The ability to conjugate and the presence of a composite antibiotic resistance island suggests that pIB_NDM_1 could both acquire more resistance genes and easily disseminate. To our knowledge, this is the first report on an untypable plasmid harbouring blaNDM-1 in P. mirabilis, in Italy.<\/jats:p>","DOI":"10.3390\/microorganisms8030339","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T06:34:23Z","timestamp":1583130863000},"page":"339","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Complete Genome and Plasmids Sequences of a Clinical Proteus mirabilis Isolate Producing Plasmid Mediated NDM-1 From Italy"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9117-3729","authenticated-orcid":false,"given":"Ibrahim","family":"Bitar","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, 32300 Pilsen, Czech Republic"},{"name":"Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vittoria","family":"Mattioni Marchetti","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Clinico-Chirurgiche, Diagnostiche e Pediatriche, Universit\u00e0 degli Studi di Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Mercato","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Clinico-Chirurgiche, Diagnostiche e Pediatriche, Universit\u00e0 degli Studi di Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Nucleo","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Clinico-Chirurgiche, Diagnostiche e Pediatriche, Universit\u00e0 degli Studi di Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano","family":"Anesi","sequence":"additional","affiliation":[{"name":"Laboratorio di Microbiologia, Azienda Socio Sanitaria Territoriale di Lodi, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Bracco","sequence":"additional","affiliation":[{"name":"Laboratorio di Microbiologia, Azienda Socio Sanitaria Territoriale di Lodi, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanina","family":"Rognoni","sequence":"additional","affiliation":[{"name":"Laboratorio di Microbiologia, Azienda Socio Sanitaria Territoriale di Lodi, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaroslav","family":"Hrabak","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, 32300 Pilsen, Czech Republic"},{"name":"Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Migliavacca","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Clinico-Chirurgiche, Diagnostiche e Pediatriche, Universit\u00e0 degli Studi di Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1128\/microbiolspec.UTI-0017-2013","article-title":"Proteus mirabilis and Urinary Tract Infections","volume":"3","author":"Schaffer","year":"2015","journal-title":"Microbiol Spectr."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Dong, D., Li, M., Liu, Z., Feng, J., Jia, N., Zhao, H., Zhao, B., Zhou, T., Zhang, X., and Tong, Y. 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