{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:51:53Z","timestamp":1791474713819,"version":"4.3.4"},"reference-count":110,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,3,22]],"date-time":"2020-03-22T00:00:00Z","timestamp":1584835200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005019","name":"Beef Cattle Research Council","doi-asserted-by":"publisher","award":["BCRC Project FOS 10.13"],"award-info":[{"award-number":["BCRC Project FOS 10.13"]}],"id":[{"id":"10.13039\/501100005019","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Genomic Research and Development Initiative-AMR program","award":["01"],"award-info":[{"award-number":["01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Antimicrobial resistance (AMR) has important implications for the continued use of antibiotics to control infectious diseases in both beef cattle and humans. AMR along the One Health continuum of the beef production system is largely unknown. Here, whole genomes of presumptive extended-spectrum \u03b2-lactamase E. coli (ESBL-EC) from cattle feces (n = 40), feedlot catch basins (n = 42), surrounding streams (n = 21), a beef processing plant (n = 4), municipal sewage (n = 30), and clinical patients (n = 25) are described. ESBL-EC were isolated from ceftriaxone selective plates and subcultured on ampicillin selective plates. Agreement of genotype-phenotype prediction of AMR ranged from 93.2% for ampicillin to 100% for neomycin, trimethoprim\/sulfamethoxazole, and enrofloxacin resistance. Overall, \u03b2-lactam (100%; blaEC, blaTEM-1, blaSHV, blaOXA, blaCTX-M-), tetracycline (90.1%; tet(A), tet(B)) and folate synthesis (sul2) antimicrobial resistance genes (ARGs) were most prevalent. The ARGs tet(C), tet(M), tet(32), blaCTX-M-1, blaCTX-M-14, blaOXA-1, dfrA18, dfrA19, catB3, and catB4 were exclusive to human sources, while blaTEM-150, blaSHV-11\u201312, dfrA12, cmlA1, and cmlA5 were exclusive to beef cattle sources. Frequently encountered virulence factors across all sources included adhesion and type II and III secretion systems, while IncFIB(AP001918) and IncFII plasmids were also common. Specificity and prevalence of ARGs between cattle-sourced and human-sourced presumptive ESBL-EC likely reflect differences in antimicrobial use in cattle and humans. Comparative genomics revealed phylogenetically distinct clusters for isolates from human vs. cattle sources, implying that human infections caused by ESBL-EC in this region might not originate from beef production sources.<\/jats:p>","DOI":"10.3390\/microorganisms8030448","type":"journal-article","created":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T04:03:01Z","timestamp":1584936181000},"page":"448","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Whole Genome Sequencing Differentiates Presumptive Extended Spectrum Beta-Lactamase Producing Escherichia coli along Segments of the One Health Continuum"],"prefix":"10.3390","volume":"8","author":[{"given":"Emelia H.","family":"Adator","sequence":"first","affiliation":[{"name":"Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Walker","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Winnipeg, MB R3E 3R2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Narvaez-Bravo","sequence":"additional","affiliation":[{"name":"Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3307-4666","authenticated-orcid":false,"given":"Rahat","family":"Zaheer","sequence":"additional","affiliation":[{"name":"Lethbridge Research and Development Centre, Lethbridge, AB T1J 4B1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Goji","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency, National Center for Animal Disease, Lethbridge Laboratory, Lethbridge, AB T1J 3Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaun R.","family":"Cook","sequence":"additional","affiliation":[{"name":"Alberta Agriculture and Forestry, Lethbridge, AB T1J 4V6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Tymensen","sequence":"additional","affiliation":[{"name":"Alberta Agriculture and Forestry, Lethbridge, AB T1J 4V6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherry J.","family":"Hannon","sequence":"additional","affiliation":[{"name":"Feedlot Health Management Services Ltd., Okotoks, AB T1S 2A2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deirdre","family":"Church","sequence":"additional","affiliation":[{"name":"Feedlot Health Management Services Ltd., Okotoks, AB T1S 2A2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7824-5065","authenticated-orcid":false,"given":"Calvin W.","family":"Booker","sequence":"additional","affiliation":[{"name":"Feedlot Health Management Services Ltd., Okotoks, AB T1S 2A2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kingsley","family":"Amoako","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency, National Center for Animal Disease, Lethbridge Laboratory, Lethbridge, AB T1J 3Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celine A.","family":"Nadon","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Winnipeg, MB R3E 3R2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron","family":"Read","sequence":"additional","affiliation":[{"name":"Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8266-6513","authenticated-orcid":false,"given":"Tim A.","family":"McAllister","sequence":"additional","affiliation":[{"name":"Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada"},{"name":"Lethbridge Research and Development Centre, Lethbridge, AB T1J 4B1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1128\/CMR.00125-13","article-title":"Escherichia coli ST131, an intriguing clonal group","volume":"27","author":"Bertrand","year":"2014","journal-title":"Clin. 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