{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T13:41:25Z","timestamp":1777642885298,"version":"3.51.4"},"reference-count":55,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T00:00:00Z","timestamp":1584921600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","award":["RTA6080004"],"award-info":[{"award-number":["RTA6080004"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Development and Promotion of Science and Technology Talents Project (Royal Government of Thailand scholarship)","award":["-"],"award-info":[{"award-number":["-"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Lipids from oleaginous microorganisms, including oleaginous yeasts, are recognized as feedstock for biodiesel production. A production process development of these organisms is necessary to bring lipid feedstock production up to the industrial scale. This study aimed to enhance lipid production of low-cost substrates, namely sugarcane top and biodiesel-derived crude glycerol, by using a two-stage cultivation process with Rhodosporidiobolus fluvialis DMKU-SP314. In the first stage, sugarcane top hydrolysate was used for cell propagation, and in the second stage, cells were suspended in a crude glycerol solution for lipid production. Optimization for high cell mass production in the first stage, and for high lipid production in the second stage, were performed separately using a one-factor-at-a-time methodology together with response surface methodology. Under optimum conditions in the first stage (sugarcane top hydrolysate broth containing; 43.18 g\/L total reducing sugars, 2.58 g\/L soy bean powder, 0.94 g\/L (NH4)2SO4, 0.39 g\/L KH2PO4 and 2.5 g\/L MgSO4 7H2O, pH 6, 200 rpm, 28 \u00b0C and 48 h) and second stage (81.54 g\/L crude glycerol, pH 5, 180 rpm, 27 \u00b0C and 196 h), a high lipid concentration of 15.85 g\/L, a high cell mass of 21.07 g\/L and a high lipid content of 73.04% dry cell mass were obtained.<\/jats:p>","DOI":"10.3390\/microorganisms8030453","type":"journal-article","created":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T07:16:08Z","timestamp":1585034168000},"page":"453","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stage"],"prefix":"10.3390","volume":"8","author":[{"given":"Jeerapan","family":"Boonyarit","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pirapan","family":"Polburee","sequence":"additional","affiliation":[{"name":"Rattanakosin College for Sustainable Energy and Environment (RCSEE), Rajamangala University of Technology Rattanakosin, Nakhon Pathom 73170, Thailand"},{"name":"Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bongkot","family":"Khaenda","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0654-1193","authenticated-orcid":false,"given":"Zongbao K.","family":"Zhao","sequence":"additional","affiliation":[{"name":"Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3744-6597","authenticated-orcid":false,"given":"Savitree","family":"Limtong","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Academy of Science, Royal Society of Thailand, Bangkok 10300, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.pecs.2016.08.001","article-title":"Biodiesel fuels","volume":"58","author":"Knothe","year":"2017","journal-title":"Prog. 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