{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:23:16Z","timestamp":1773793396871,"version":"3.50.1"},"reference-count":81,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T00:00:00Z","timestamp":1585785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003569","name":"Ministry of Food and Drug Safety","doi-asserted-by":"publisher","award":["15161MFDS645"],"award-info":[{"award-number":["15161MFDS645"]}],"id":[{"id":"10.13039\/501100003569","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003569","name":"Ministry of Food and Drug Safety","doi-asserted-by":"publisher","award":["20161MFDS009"],"award-info":[{"award-number":["20161MFDS009"]}],"id":[{"id":"10.13039\/501100003569","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The spread of extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) has posed a critical health risk to both humans and animals, because resistance to beta-lactam antibiotics makes treatment for commonly infectious diseases more complicated. In this study, we report the prevalence and genetic characteristics of ESBL-ECs isolated from retail meat samples in Korea. A total of 1205 E. coli strains were isolated from 3234 raw meat samples, purchased from nationwide retail stores between 2015 and 2018. Antimicrobial susceptibility testing was performed for all isolates by a broth microdilution method, and the ESBL phenotype was determined according to the Clinical and Laboratory Standards Institute (CLSI) confirmatory method. All ESBL-EC isolates (n = 29) were subjected to whole-genome sequencing (WGS). The antimicrobial resistance genes, plasmid incompatibility types, E. coli phylogroups, and phylogenetic relations were investigated based on the WGS data. The prevalence of ESBL-ECs in chicken was significantly higher than that in other meat samples. The results in this study demonstrate that clonally diverse ESBL-ECs with a multidrug resistance phenotype were distributed nationwide, although their prevalence from retail meat was 0.9%. The dissemination of ESBL-ECs from retail meat poses a potential risk to consumers and food-handlers, suggesting that the continuous surveillance of ESBL-ECs in retail meat should be conducted at the national level.<\/jats:p>","DOI":"10.3390\/microorganisms8040508","type":"journal-article","created":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T11:57:14Z","timestamp":1585828634000},"page":"508","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolated from Retail Meats in Korea"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1859-5703","authenticated-orcid":false,"given":"Seokhwan","family":"Kim","sequence":"first","affiliation":[{"name":"Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Korea"},{"name":"Department of Food and Animal Biotechnology, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1177-180X","authenticated-orcid":false,"given":"Hansol","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonghoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Migyeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyosun","family":"Kwak","sequence":"additional","affiliation":[{"name":"Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5812-3394","authenticated-orcid":false,"given":"Sangryeol","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Food and Animal Biotechnology, Seoul National University, Seoul 08826, Korea"},{"name":"Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1038\/nrmicro2265","article-title":"Molecular mechanisms of Escherichia coli pathogenicity","volume":"8","author":"Croxen","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nrmicro818","article-title":"Pathogenic Escherichia coli","volume":"2","author":"Kaper","year":"2004","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3568","DOI":"10.1128\/AAC.06330-11","article-title":"Efficacy of bacteriophage therapy in experimental sepsis and meningitis caused by a clone O25b: H4-ST131 Escherichia coli strain producing CTX-M-15","volume":"56","author":"Pouillot","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1046\/j.1365-2672.2003.01988.x","article-title":"Transfer of antibiotic resistance between commensal and pathogenic members of the Enterobacteriaceae under ileal conditions","volume":"95","author":"Blake","year":"2003","journal-title":"J. Appl. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"258","DOI":"10.3389\/fmicb.2013.00258","article-title":"Multidrug resistant commensal Escherichia coli in animals and its impact for public health","volume":"4","author":"Szmolka","year":"2013","journal-title":"Front. Microbiol."},{"key":"ref_6","unstructured":"World Health Organization (2017). Integrated Surveillance of Antimicrobial Resistance in Foodborne Bacteria: Application of a One Health Approach: Guidance from the WHO Advisory Group on Integrated Surveillance of Antimicrobial Resistance (AGISAR), WHO."},{"key":"ref_7","unstructured":"World Health Organization (2017). Global Priority of Antibiotic-Resistant Bacteria to Guide Research, Discovery, and Development of New Antibiotics, WHO."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.18.4.657-686.2005","article-title":"Extended-spectrum \u03b2-lactamases: A clinical update","volume":"18","author":"Paterson","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1016\/j.cell.2014.11.017","article-title":"Beta-lactam antibiotics induce a lethal malfunctioning of the bacterial cell wall synthesis machinery","volume":"159","author":"Cho","year":"2014","journal-title":"Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"iii2","DOI":"10.1093\/jac\/dky027","article-title":"Treatment of infections caused by multidrug-resistant gram-negative bacteria: Report of the British Society for Antimicrobial Chemotherapy\/healthcare Infection Society\/British Infection Association Joint Working Party","volume":"73","author":"Hawkey","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Ba\u00f1o, J., Guti\u00e9rrez-Guti\u00e9rrez, B., Machuca, I., and Pascual, A. (2018). Treatment of infections caused by extended-spectrum-beta-lactamase-, AmpC-, and carbapenemase-producing Enterobacteriaceae. Clin. Microbiol. Rev., 31.","DOI":"10.1128\/CMR.00079-17"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1093\/jac\/dkl483","article-title":"CTX-M: Changing the face of ESBLs in Europe","volume":"59","author":"Livermore","year":"2006","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.plasmid.2018.09.010","article-title":"Plasmid-mediated resistance is going wild","volume":"99","author":"Dolejska","year":"2018","journal-title":"Plasmid"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"S44","DOI":"10.1093\/jtm\/taw102","article-title":"The ecology of extended-spectrum \u03b2-lactamases (ESBLs) in the developed world","volume":"24","author":"Doi","year":"2017","journal-title":"J. Travel Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.ijmm.2014.07.015","article-title":"Subgrouping of ESBL-producing Escherichia coli from animal and human sources: An approach to quantify the distribution of ESBL types between different reservoirs","volume":"304","author":"Valentin","year":"2014","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"De Been, M., Lanza, V.F., De Toro, M., Scharringa, J., Dohmen, W., Du, Y., Hu, J., Lei, Y., Li, N., and Tooming-Klunderud, A. (2014). Dissemination of cephalosporin resistance genes between Escherichia coli strains from farm animals and humans by specific plasmid lineages. PLoS Genet., 10.","DOI":"10.1371\/journal.pgen.1004776"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1093\/cid\/cis1043","article-title":"Public health risks of enterobacterial isolates producing extended-spectrum \u03b2-lactamases or AmpC \u03b2-lactamases in food and food-producing animals: An EU perspective of epidemiology, analytical methods, risk factors, and control options","volume":"56","author":"Liebana","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1111\/j.1469-0691.2011.03497.x","article-title":"Dutch patients, retail chicken meat and poultry share the same ESBL genes, plasmids and strains","volume":"17","author":"Dierikx","year":"2011","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1093\/cid\/ciu785","article-title":"Do human extraintestinal Escherichia coli infections resistant to expanded-spectrum cephalosporins originate from food-producing animals? A systematic review","volume":"60","author":"Lazarus","year":"2014","journal-title":"Clin. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/S0140-6736(15)00473-0","article-title":"Understanding the mechanisms and drivers of antimicrobial resistance","volume":"387","author":"Holmes","year":"2016","journal-title":"Lancet"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/S0924-8579(01)00297-7","article-title":"Use of antimicrobial agents in veterinary medicine and food animal production","volume":"17","author":"Schwarz","year":"2001","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Oniciuc, E.A., Likotrafiti, E., Alvarez-Molina, A., Prieto, M., Santos, J.A., and Alvarez-Ord\u00f3\u00f1ez, A. (2018). The present and future of whole genome sequencing (WGS) and whole metagenome sequencing (WMS) for surveillance of antimicrobial resistant microorganisms and antimicrobial resistance genes across the food chain. Genes, 9.","DOI":"10.3390\/genes9050268"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1089\/mdr.2018.0237","article-title":"First detection of New Delhi metallo-\u03b2-Lactamase-5-producing Escherichia coli from companion animals in Korea","volume":"25","author":"Hong","year":"2019","journal-title":"Microb. Drug. Resist."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1089\/fpd.2014.1921","article-title":"Characterization of Escherichia coli\u2013producing extended-spectrum \u03b2-lactamase (ESBL) Isolated from chicken slaughterhouses in South Korea","volume":"12","author":"Lim","year":"2015","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"421","DOI":"10.4014\/jmb.1401.01023","article-title":"Characterization of CTX-M-type extended-spectrum beta-lactamase-producing diarrheagenic Escherichia coli isolates in the Republic of Korea during 2008\u20132011","volume":"24","author":"Kim","year":"2014","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1089\/fpd.2012.1245","article-title":"Prevalence and molecular characterization of CTX-M \u03b2-lactamase\u2013producing Escherichia coli isolated from healthy swine and cattle","volume":"10","author":"Tamang","year":"2013","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3898","DOI":"10.1128\/AEM.00522-13","article-title":"Molecular characterization of CTX-M \u03b2-lactamase and associated addiction systems in Escherichia coli circulating among cattle, farm workers, and the farm environment","volume":"79","author":"Tamang","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.femsle.2005.02.029","article-title":"CTX-M and SHV-12 \u03b2-lactamases are the most common extended-spectrum enzymes in clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 3 university hospitals within Korea","volume":"245","author":"Kim","year":"2005","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"180","DOI":"10.3343\/alm.2020.40.2.180","article-title":"Whole-genome analysis of blaCTX-M-55-carrying Escherichia coli among pigs, farm environment, and farm workers","volume":"40","author":"Kim","year":"2020","journal-title":"Ann. Lab. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.jgar.2019.01.005","article-title":"Predominance of blaCTX-M-65 and blaCTX-M-55 in extended-spectrum \u03b2-lactamase-producing Escherichia coli from raw retail chicken in South Korea","volume":"17","author":"Park","year":"2019","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.foodres.2017.12.023","article-title":"Genotypic characterization of ESBL-producing Escherichia coli from imported meat in South Korea","volume":"107","author":"Kim","year":"2018","journal-title":"Food Res. Int."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"270","DOI":"10.4014\/jmb.1507.07048","article-title":"Molecular characterization of plasmids encoding CTX-M \u03b2-lactamases and their associated addiction systems circulating among Escherichia coli from retail chickens, chicken farms, and slaughterhouses in Korea","volume":"26","author":"Jo","year":"2016","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_33","unstructured":"CLSI (2019). Performance Standards for Antimicrobial Susceptibility Testing. CLSI Supplement M100, Clinical and Laboratory Standards Institute. [29th ed.]."},{"key":"ref_34","unstructured":"EUCAST (2019, January 30). The European Committee on Antimicrobial Susceptibility Testing. Breakpoint Tables for Interpretation of MICs and Zone Diameters. Version 9.0. Available online: http:\/\/www.eucast.org."},{"key":"ref_35","unstructured":"NARMS (2019, May 23). The National Antimicrobial Resistance Monitoring System. Antimicrobial Agents Used for Susceptibility Testing for Escherichia coli Isolates, Available online: https:\/\/www.cdc.gov\/narms\/antibiotics-tested.html."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: Rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: Rapid large-scale prokaryote pan genome analysis","volume":"31","author":"Page","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.mib.2008.09.006","article-title":"Comparative genomics: The bacterial pan-genome","volume":"11","author":"Tettelin","year":"2008","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6881","DOI":"10.1128\/JB.00619-08","article-title":"The pangenome structure of Escherichia coli: Comparative genomic analysis of E. coli commensal and pathogenic isolates","volume":"190","author":"Rasko","year":"2008","journal-title":"J. Bacteriol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"459","DOI":"10.3389\/fcimb.2017.00459","article-title":"Comparative pan-genome analysis of Piscirickettsia salmonis reveals genomic divergences within genogroups","volume":"7","author":"Molina","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1093\/bioinformatics\/btu017","article-title":"PanGP: A tool for quickly analyzing bacterial pan-genome profile","volume":"30","author":"Zhao","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Page, A.J., Taylor, B., Delaney, A.J., Soares, J., Seemann, T., Keane, J.A., and Harris, S.R. (2016). SNP-sites: Rapid efficient extraction of SNPs from multi-FASTA alignments. Microb. Genom., 2.","DOI":"10.1099\/mgen.0.000056"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1093\/molbev\/mst028","article-title":"Hierarchical and spatially explicit clustering of DNA sequences with BAPS software","volume":"30","author":"Cheng","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1093\/bioinformatics\/btx610","article-title":"Phandango: An interactive viewer for bacterial population genomics","volume":"34","author":"Hadfield","year":"2017","journal-title":"Bioinformatics"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Beghain, J., Bridier-Nahmias, A., Le Nagard, H., Denamur, E., and Clermont, O. (2018). ClermonTyping: An easy-to-use and accurate in silico method for Escherichia genus strain phylotyping. Microb. Genom., 4.","DOI":"10.1099\/mgen.0.000192"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/AAC.02412-14","article-title":"In silico detection and typing of plasmids using PlasmidFinder and Plasmid Multilocus Sequence Typing","volume":"58","author":"Carattoli","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.1093\/jac\/dks261","article-title":"Identification of acquired antimicrobial resistance genes","volume":"67","author":"Zankari","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1111\/j.1365-2958.2006.05172.x","article-title":"Sex and virulence in Escherichia coli: An evolutionary perspective","volume":"60","author":"Wirth","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1128\/JCM.06094-11","article-title":"Multilocus sequence typing of total-genome-sequenced bacteria","volume":"50","author":"Larsen","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.4014\/jmb.1610.10006","article-title":"Characteristics of transmissible CTX-M- and CMY-type \u03b2-lactamase-producing Escherichia coli isolates collected from pig and chicken farms in South Korea","volume":"27","author":"Shin","year":"2017","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.vetmic.2019.03.025","article-title":"Diversity in prevalence and characteristics of ESBL\/pAmpC producing E. coli in food in Germany","volume":"233","author":"Kaesbohrer","year":"2019","journal-title":"Vet. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.ijfoodmicro.2016.10.036","article-title":"Evaluation of meat, fruit and vegetables from retail stores in five United Kingdom regions as sources of extended-spectrum beta-lactamase (ESBL)-producing and carbapenem-resistant Escherichia coli","volume":"241","author":"Randall","year":"2017","journal-title":"Int. J. Food Microbiol."},{"key":"ref_55","first-page":"M2023","article-title":"Prevalence of \u03b2-lactamase producing Escherichia coli from retail meat in Turkey","volume":"80","year":"2015","journal-title":"J. Food Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.meatsci.2012.09.009","article-title":"Prevalence of extended-spectrum \u03b2-lactamase-producing Enterobacteriaceae in meat products sold in Navarra, Spain","volume":"93","author":"Vitas","year":"2013","journal-title":"Meat Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/S1473-3099(19)30431-1","article-title":"Bloodstream infections and carbapenem-resistant Enterobacteriaceae in South Korea","volume":"19","author":"Yoon","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.ajic.2014.02.024","article-title":"Risk factors for the acquisition of carbapenem-resistant Escherichia coli at a tertiary care center in South Korea: A matched case-control study","volume":"42","author":"Ahn","year":"2014","journal-title":"Am. J. Infect. Control"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"eaaw1944","DOI":"10.1126\/science.aaw1944","article-title":"Global trends in antimicrobial resistance in animals in low-and middle-income countries","volume":"365","author":"Pires","year":"2019","journal-title":"Science"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1093\/jac\/dkx146","article-title":"Global epidemiology of CTX-M \u03b2-lactamases: Temporal and geographical shifts in genotype","volume":"72","author":"Bevan","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.3389\/fmicb.2019.01371","article-title":"Clonal spread of extended-spectrum cephalosporin-resistant Enterobacteriaceae between companion animals and humans in South Korea","volume":"10","author":"Hong","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1089\/fpd.2019.2644","article-title":"Antimicrobial resistance and molecular characterization of extended-spectrum \u03b2-lactamase-producing Escherichia coli isolated from ducks in South Korea","volume":"16","author":"Na","year":"2019","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"5976","DOI":"10.1128\/AAC.04920-14","article-title":"Residues distal to the active site contribute to enhanced catalytic activity of variant and hybrid \u03b2-lactamases derived from CTX-M-14 and CTX-M-15","volume":"59","author":"He","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.diagmicrobio.2007.02.005","article-title":"The emergence of a novel ceftazidime-resistant CTX-M extended-spectrum \u03b2-lactamase, CTX-M-55, in both community-onset and hospital-acquired infections in Thailand","volume":"58","author":"Kiratisin","year":"2007","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1093\/jac\/dky451","article-title":"CTX-M-55-type ESBL-producing Salmonella enterica are emerging among retail meats in Phnom Penh, Cambodia","volume":"74","author":"Nadimpalli","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.vetmic.2014.06.013","article-title":"Increasing prevalence of extended-spectrum cephalosporin-resistant Escherichia coli in food animals and the diversity of CTX-M genotypes during 2003\u20132012","volume":"172","author":"Rao","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.ijantimicag.2011.12.001","article-title":"Prevalence and characterisation of CTX-M \u03b2-lactamases amongst Escherichia coli isolates from healthy food animals in China","volume":"39","author":"Zheng","year":"2012","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.ijfoodmicro.2018.08.003","article-title":"High prevalence of blaCTX-M-14 among genetically diverse Escherichia coli recovered from retail raw chicken meat portions in Japan","volume":"284","author":"Hayashi","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1128\/CMR.00116-14","article-title":"The role of epidemic resistance plasmids and international high-risk clones in the spread of multidrug-resistant Enterobacteriaceae","volume":"28","author":"Mathers","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1093\/jac\/dkx058","article-title":"Genomic landscape of extended-spectrum \u03b2-lactamase resistance in Escherichia coli from an urban African setting","volume":"72","author":"Musicha","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"834","DOI":"10.3389\/fmicb.2019.00834","article-title":"Large-scale genomics reveals the genetic characteristics of seven species and importance of phylogenetic distance for estimating pan-genome size","volume":"10","author":"Park","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1093\/jac\/dky506","article-title":"Limited contribution of non-intensive chicken farming to ESBL-producing Escherichia coli colonization in humans in Vietnam: An epidemiological and genomic analysis","volume":"74","author":"Nguyen","year":"2019","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.mimet.2014.03.008","article-title":"Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing","volume":"101","author":"Clermont","year":"2014","journal-title":"J. Microbiol. Methods"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-22943-0","article-title":"Impact of human-associated Escherichia coli clonal groups in antarctic pinnipeds: Presence of ST73, ST95, ST141 and ST131","volume":"8","author":"Mora","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.cmi.2015.11.010","article-title":"Escherichia coli and urinary tract infections: The role of poultry-meat","volume":"22","author":"Manges","year":"2016","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1016\/S1473-3099(19)30273-7","article-title":"Extended-spectrum \u03b2-lactamase-producing Escherichia coli in human-derived and foodchain-derived samples from England, Wales, and Scotland: An epidemiological surveillance and typing study","volume":"19","author":"Day","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1007\/s15010-013-0441-z","article-title":"Clinical characteristics of community-acquired acute pyelonephritis caused by ESBL-producing pathogens in South Korea","volume":"41","author":"Kim","year":"2013","journal-title":"Infection"},{"key":"ref_78","unstructured":"Villa, M.F.L., Salinas, L., Villavivencio, F., Rafael, T., Salas, S., Rivera, R., Villacis, J., Satan, C., Ushi\u00f1a, L., and Mu\u00f1oz, O. (2018). Diverse Escherichia coli lineages, from domestic animals and humans in a household, carry colistin resistance gene mcr-1 in Ecuador. bioRxiv."},{"key":"ref_79","first-page":"3298","article-title":"Longitudinal study of ESBL Escherichia coli carriage on an organic broiler farm","volume":"73","author":"Veenman","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1093\/molbev\/msv280","article-title":"Emergence of antimicrobial-resistant Escherichia coli of animal origin spreading in humans","volume":"33","author":"Skurnik","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"44","DOI":"10.4167\/jbv.2014.44.1.44","article-title":"Comparisons of CTX-M-producing Escherichia coli isolates from humans and animals in South Korea","volume":"44","author":"Choi","year":"2014","journal-title":"J. Bacteriol. Virol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/4\/508\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:14:52Z","timestamp":1760174092000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/4\/508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,2]]},"references-count":81,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["microorganisms8040508"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms8040508","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,2]]}}}