{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:14:02Z","timestamp":1790356442292,"version":"4.1.0"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["16-34238A"],"award-info":[{"award-number":["16-34238A"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["CZ.02.1.01\/0.0\/0.0\/15_003\/0000495"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/15_003\/0000495"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Vector-borne flaviviruses (VBFs) affect human health worldwide, but no approved drugs are available specifically to treat VBF-associated infections. Here, we performed in silico screening of a library of U.S. Food and Drug Administration-approved antiviral drugs for their interaction with Zika virus proteins. Twelve hit drugs were identified by the docking experiments and tested in cell-based antiviral assay systems. Efavirenz, tipranavir, and dasabuvir at micromolar concentrations were identified to inhibit all VBFs tested; i.e., two representatives of mosquito-borne flaviviruses (Zika and West Nile viruses) and one representative of flaviviruses transmitted by ticks (tick-borne encephalitis virus). The results warrant further research into these drugs, either individually or in combination, as possible pan-flavivirus inhibitors.<\/jats:p>","DOI":"10.3390\/microorganisms8040599","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T03:23:06Z","timestamp":1587439386000},"page":"599","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["FDA-Approved Drugs Efavirenz, Tipranavir, and Dasabuvir Inhibit Replication of Multiple Flaviviruses in Vero Cells"],"prefix":"10.3390","volume":"8","author":[{"given":"Michal","family":"Stefanik","sequence":"first","affiliation":[{"name":"Department of Virology, Veterinary Research Institute, Hudcova 70, CZ-62100 Brno, Czech Republic"},{"name":"Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James J.","family":"Valdes","sequence":"additional","affiliation":[{"name":"Department of Virology, Veterinary Research Institute, Hudcova 70, CZ-62100 Brno, Czech Republic"},{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 31, CZ-37005 Ceske Budejovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-1215","authenticated-orcid":false,"given":"Fortunatus C.","family":"Ezebuo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, PMB 5025 Awka 420281, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Haviernik","sequence":"additional","affiliation":[{"name":"Department of Virology, Veterinary Research Institute, Hudcova 70, CZ-62100 Brno, Czech Republic"},{"name":"Faculty of Science, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5139-1537","authenticated-orcid":false,"given":"Ikemefuna C.","family":"Uzochukwu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, PMB 5025 Awka 420281, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Fojtikova","sequence":"additional","affiliation":[{"name":"Department of Virology, Veterinary Research Institute, Hudcova 70, CZ-62100 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Salat","sequence":"additional","affiliation":[{"name":"Department of Virology, Veterinary Research Institute, Hudcova 70, CZ-62100 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludek","family":"Eyer","sequence":"additional","affiliation":[{"name":"Department of Virology, Veterinary Research Institute, Hudcova 70, CZ-62100 Brno, Czech Republic"},{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 31, CZ-37005 Ceske Budejovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4655-2380","authenticated-orcid":false,"given":"Daniel","family":"Ruzek","sequence":"additional","affiliation":[{"name":"Department of Virology, Veterinary Research Institute, Hudcova 70, CZ-62100 Brno, Czech Republic"},{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 31, CZ-37005 Ceske Budejovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1099\/jgv.0.000672","article-title":"Ictv Report Consortium. 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