{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T01:10:15Z","timestamp":1768871415331,"version":"3.49.0"},"reference-count":29,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T00:00:00Z","timestamp":1587600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012538","name":"Swedish Cancer Foundation","doi-asserted-by":"publisher","award":["180760"],"award-info":[{"award-number":["180760"]}],"id":[{"id":"10.13039\/100012538","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Acinetobacter baumannii is an opportunistic bacterial pathogen associated with hospital-acquired infections, including pneumonia, meningitis, bacteremia, urinary tract infection, and wound infections. Recognition of host cell surface carbohydrates plays a crucial role in adhesion and enables microbes to colonize different host niches. Here the potential glycosphingolipid receptors of A. baumannii were examined by binding of 35S-labeled bacteria to glycosphingolipids on thin-layer chromatograms. Thereby a selective interaction with two non-acid glycosphingolipids of human and rabbit small intestine was found. The binding-active glycosphingolipids were isolated and, on the basis of mass spectrometry, identified as neolactotetraosylceramide (Gal\u03b24GlcNAc\u03b23Gal\u03b24Glc\u03b21Cer) and lactotetraosylceramide (Gal\u03b23GlcNAc\u03b23Gal\u03b24Glc\u03b21Cer). Further binding assays using reference glycosphingolipids showed that A. baumannii also bound to lactotriaosylceramide (GlcNAc\u03b23Gal\u03b24Glc\u03b21Cer) demonstrating that GlcNAc was the basic element recognized. In addition, the bacteria occasionally bound to galactosylceramide, lactosylceramide with phytosphingosine and\/or hydroxy fatty acids, isoglobotriaosylceramide, gangliotriaosylceramide, and gangliotetraosylceramide, in analogy with binding patterns that previously have been described for other bacteria classified as \u201clactosylceramide-binding\u201d. Finally, by isolation and characterization of glycosphingolipids from human skin, the presence of neolactotetraosylceramide was demonstrated in this A. baumannii target tissue.<\/jats:p>","DOI":"10.3390\/microorganisms8040612","type":"journal-article","created":{"date-parts":[[2020,4,24]],"date-time":"2020-04-24T11:42:14Z","timestamp":1587728534000},"page":"612","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Glycosphingolipids Recognized by Acinetobacter baumannii"],"prefix":"10.3390","volume":"8","author":[{"given":"Miralda","family":"Madar Johansson","sequence":"first","affiliation":[{"name":"Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, Sahlgrenska Academy, University of Gothenburg, SE40530 G\u00f6teborg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehjar","family":"Azzouz","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, Sahlgrenska Academy, University of Gothenburg, SE40530 G\u00f6teborg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatrice","family":"H\u00e4ggendal","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, Sahlgrenska Academy, University of Gothenburg, SE40530 G\u00f6teborg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2839-9362","authenticated-orcid":false,"given":"Karin","family":"S\u00e4lj\u00f6","sequence":"additional","affiliation":[{"name":"Institute of Clinical Sciences, Department of Plastic Surgery, Sahlgrenska Academy, University of Gothenburg, SE41345 G\u00f6teborg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henri","family":"Malmi","sequence":"additional","affiliation":[{"name":"Joint Biotechnology Laboratory, MediCity, Faculty of Medicine, University of Turku, FI25020 Turku, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6191-5931","authenticated-orcid":false,"given":"Anton","family":"Zavialov","sequence":"additional","affiliation":[{"name":"Joint Biotechnology Laboratory, MediCity, Faculty of Medicine, University of Turku, FI25020 Turku, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susann","family":"Teneberg","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, Sahlgrenska Academy, University of Gothenburg, SE40530 G\u00f6teborg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,23]]},"reference":[{"key":"ref_1","unstructured":"(2017, February 27). World Health Organization, WHO Publishes List of Bacteria for Which New Antibiotics Are Urgently Needed. Available online: http:\/\/www.who.int\/mediacentre\/news\/releases\/2017\/bacteria-antibiotics-needed\/en\/."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jhin.2011.08.013","article-title":"Effect of biofilm formation on the survival of Acinetobacter baumannii on dry surfaces","volume":"80","author":"Espinal","year":"2012","journal-title":"J. Hospital. Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3473","DOI":"10.1099\/mic.0.26541-0","article-title":"Attachment to and biofilm formation on abiotic surfaces by Acinetobacter baumannii: Involvement of a novel chaperone-usher pili assembly system","volume":"149","author":"Tomaras","year":"2003","journal-title":"Microbiology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.resmic.2009.01.002","article-title":"CsuA\/BABCDE-dependent pili are not involved in the adherence of Acinetobacter baumannii ATCC19606T to human airway epithelial cells and their inflammatory response","volume":"160","author":"Gaddy","year":"2009","journal-title":"Res. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5558","DOI":"10.1073\/pnas.1800961115","article-title":"Structural basis for Acinetobacter baumannii biofilm formation","volume":"115","author":"Pakharukova","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3150","DOI":"10.1128\/IAI.00096-09","article-title":"The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells","volume":"77","author":"Gaddy","year":"2009","journal-title":"Infect. Immun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1128\/IAI.05913-11","article-title":"The Acinetobacter baumannii biofilm-associated protein plays a role in adherence to human epithelial cells","volume":"80","author":"Brossard","year":"2012","journal-title":"Infect. Immun."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"De Gregorio, E., Del Franco, M., Martinucci, M., Roscetto, E., Zarrilli, R., and Di Nocera, P.P. (2015). Biofilm-associated proteins: News from Acinetobacter. BMC Genom., 16.","DOI":"10.1186\/s12864-015-2136-6"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1080\/21505594.2016.1145335","article-title":"Analysis of the role of the LH92_11085 gene of a biofilm hyperproducing Acinetobacter baumannii strain on biofilm formation and attachment to eukaryotic cells","volume":"7","author":"Merino","year":"2016","journal-title":"Virulence"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1080\/21505594.2018.1558693","article-title":"The Acinetobacter trimeric autotransporter adhesin Ata controls key virulence traits of Acinetobacter baumannii","volume":"10","author":"Weidensdorfer","year":"2019","journal-title":"Virulence"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.resmic.2005.09.011","article-title":"Adherence of Acinetobacter baumannii strains to human bronchial epithelial cells","volume":"157","author":"Lee","year":"2006","journal-title":"Res. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Giannouli, M., Antunes, L.C., Marchetti, V., Triassi, M., Visca, P., and Zarrilli, R. (2013). Virulence-related traits of epidemic Acinetobacter baumannii strains belonging to the international clonal lineages I-III and to the emerging genotypes ST25 and ST78. BMC Infect. Dis., 13.","DOI":"10.1186\/1471-2334-13-282"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/978-94-007-0940-9_14","article-title":"Carbohydrate mediated bacterial adhesion","volume":"715","author":"Pieters","year":"2011","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1146\/annurev.bi.58.070189.001521","article-title":"Animal glycosphingolipids as membrane attachment sites for bacteria","volume":"58","author":"Karlsson","year":"1989","journal-title":"Annu. Rev. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/0076-6879(87)38018-8","article-title":"Preparation of total non-acid glycolipids for overlay analysis of receptors for bacteria and viruses and for other studies","volume":"138","author":"Karlsson","year":"1987","journal-title":"Meth. Enzymol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/0076-6879(90)93442-N","article-title":"Mass spectrometry of mixtures of intact glycosphingolipids","volume":"193","author":"Samuelsson","year":"1990","journal-title":"Meth. Enzymol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1093\/glycob\/cwq070","article-title":"Differentiation of glycosphingolipid-derived glycan structural isomers by liquid chromatography-mass spectrometry","volume":"20","author":"Karlsson","year":"2010","journal-title":"Glycobiology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2676","DOI":"10.1021\/bi00280a014","article-title":"High-resolution proton NMR studies of gangliosides. 1. Use of homonuclear spin-echo J-correlated spectroscopy for determination of residue composition and anomeric configurations","volume":"22","author":"Koerner","year":"1983","journal-title":"Biochemistry"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Stahl, E. (1962). Spr\u00fchreagentien f\u00fcr die d\u00fcnnschicht-chromatographie. D\u00fcnnschicht-Chromatographie, Springer.","DOI":"10.1007\/978-3-642-45993-1_8"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1128\/IAI.72.3.1519-1529.2004","article-title":"Helicobacter pylori and complex gangliosides","volume":"72","author":"Roche","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"8554","DOI":"10.1016\/S0021-9258(17)37231-9","article-title":"Characterization of binding of Gal\u03b24GlcNAc-specific lectins from Erythrina christagalli and Erythrina corallodendron to glycosphingolipids. Detection, isolation and characteriztion of a novel glycosphingolipid of bovine buttermilk","volume":"269","author":"Teneberg","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1021\/ac0010126","article-title":"Negative ion electrospray mass spectrometry of neutral underivatized oligosaccharides","volume":"73","author":"Chai","year":"2001","journal-title":"Anal. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1002\/rcm.1352","article-title":"Diagnostic ions for the rapid analysis by nano-electrospray ionization quadrupole time.of.flight mass spectrometry of O-glycans from human mucins","volume":"18","author":"Robbe","year":"2004","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1111\/j.1346-8138.1983.tb01179.x","article-title":"The glycolipids of normal human skin","volume":"10","author":"Hamanaka","year":"1983","journal-title":"J. Dermatol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/j.1574-6976.2012.00344.x","article-title":"Acinetobacter baumannii: Human infections, factors contributing to pathogenesis and animal models","volume":"37","author":"McConnell","year":"2013","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/j.jmb.2008.08.028","article-title":"Structural basis of the preferential binding for globo-series glycosphingolipids displayed by Pseudomonas aeruginosa lectin I","volume":"383","author":"Blanchard","year":"2008","journal-title":"J. Mol. Biol."},{"key":"ref_27","first-page":"3085","article-title":"The intracellular localization of Pseudomonas aeruginosa lectins","volume":"129","author":"Glick","year":"1983","journal-title":"J. Gen. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/S0022-2836(03)00841-6","article-title":"The structural basis of receptor binding by Escherichia coli associated with diarrhea and septicemia","volume":"331","author":"Merckel","year":"2003","journal-title":"J. Mol. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1046\/j.1365-2958.2003.03600.x","article-title":"The fimbrial adhesin F17-G of enterotoxigenic Escherichia coli has an immunoglobulin-like lectin domain that binds N-acetylglucosamine","volume":"49","author":"Buts","year":"2003","journal-title":"Mol. Microbiol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/4\/612\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:27:08Z","timestamp":1760365628000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/4\/612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,23]]},"references-count":29,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["microorganisms8040612"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms8040612","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,23]]}}}