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We screened a library containing 1600 clinically tested drug compounds to identify compounds that inhibit C. albicans biofilm formation. The compounds that emerged from the initial screen were validated in a secondary screen and then tested for (1) their abilities to disrupt mature biofilms and (2) for synergistic interactions with representatives of the three antifungal agents most commonly prescribed to treat Candida infections, fluconazole, amphotericin B, and caspofungin. Twenty compounds had antibiofilm activity in at least one of the secondary assays and several affected biofilms but, at the same concentration, had little or no effect on planktonic (suspension) growth of C. albicans. Two calcium channel blockers, a selective serotonin reuptake inhibitor, and an azole-based proton pump inhibitor were among the hits, suggesting that members of these three classes of drugs or their derivatives may be useful for treating C. albicans biofilm infections.<\/jats:p>","DOI":"10.3390\/microorganisms8050756","type":"journal-article","created":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T11:34:14Z","timestamp":1589801654000},"page":"756","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Selective Serotonin Reuptake Inhibitor, a Proton Pump Inhibitor, and Two Calcium Channel Blockers Inhibit Candida albicans Biofilms"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0799-6499","authenticated-orcid":false,"given":"Clarissa J.","family":"Nobile","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Natural Sciences, University of California\u2014Merced, Merced, CA 95343, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6497-0397","authenticated-orcid":false,"given":"Craig L.","family":"Ennis","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Natural Sciences, University of California\u2014Merced, Merced, CA 95343, USA"},{"name":"Quantitative and Systems Biology Graduate Program, University of California\u2014Merced, Merced, CA 95343, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nairi","family":"Hartooni","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California\u2014San Francisco, San Francisco, CA 94158, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander D.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California\u2014San Francisco, San Francisco, CA 94158, USA"},{"name":"Department of Biochemistry and Biophysics, University of California\u2014San Francisco, San Francisco, CA 94158, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew B.","family":"Lohse","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California\u2014San Francisco, San Francisco, CA 94158, USA"},{"name":"Department of Biology, BioSynesis, Inc., San Francisco, CA 94114, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/S0966-842X(02)00002-1","article-title":"Candida biofilms and their role in infection","volume":"11","author":"Douglas","year":"2003","journal-title":"Trends Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1146\/annurev-micro-091014-104330","article-title":"Candida albicans Biofilms and Human Disease","volume":"69","author":"Nobile","year":"2015","journal-title":"Annu. 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