{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T17:03:54Z","timestamp":1787418234832,"version":"build-2736575974"},"reference-count":53,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T00:00:00Z","timestamp":1590105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Background: Escherichia coli and Salmonella are etiologic agents of intestinal infections. A previous study showed the presence of shared epitopes between lipopolysaccharides (LPSs) of E. coli O157 and Salmonella. Aim: Using phage display, the aim of this study is to identify mimotopes of shared epitopes in different enterobacterial LPSs. Methods: We use anti-LPS IgG from E. coli O157 and Salmonella to select peptide mimotopes of the M13 phage. The amino acid sequence of the mimotopes is used to synthesize peptides, which are in turn used to immunize rabbits. The antibody response of the resulting sera against the LPSs and synthetic peptides (SPs) is analyzed by ELISA and by Western blot assays, indicating that LPS sites are recognized by the same antibody. In a complementary test, the reactions of human serum samples obtained from the general population against the SPs and LPSs are also analyzed. Results: From the last biopanning phase, sixty phagotopes are selected. The analysis of the peptide mimotope amino acid sequences shows that in 4 of them the S\/N\/A\/PF motif is a common sequence. Antibodies from the sera of immunized rabbits with SP287\/3, SP459\/1, SP308\/3, and SP073\/14 react against both their own peptide and the different LPSs. The Western blot test shows a sera reaction against both the lateral chains and the cores of the LPSs. The analysis of the human sera shows a response against the SPs and LPSs. Conclusion: The designed synthetic peptides are mimotopes of LPS epitopes of Salmonella and E. coli that possess immunogenic capacity. These mimotopes could be considered for use in the design of vaccines against both enterobacteria.<\/jats:p>","DOI":"10.3390\/microorganisms8050780","type":"journal-article","created":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T10:18:18Z","timestamp":1590142698000},"page":"780","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6693-7168","authenticated-orcid":false,"given":"Armando","family":"Navarro","sequence":"first","affiliation":[{"name":"Departamento de Salud P\u00fablica, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City CP 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delia","family":"Licona-Moreno","sequence":"additional","affiliation":[{"name":"Departamento de Salud P\u00fablica, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City CP 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Monsalvo-Reyes","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica Molecular, Unidad de Biolog\u00eda y Prototipos, UBIPRO, Facultad de Estudios Superiores Iztacala, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Los Reyes Ixtacala Tlalnepantla, Estado de M\u00e9xico P C 54090, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4708-8807","authenticated-orcid":false,"given":"Ulises","family":"Hern\u00e1ndez-Chi\u00f1as","sequence":"additional","affiliation":[{"name":"Departamento de Salud P\u00fablica, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City CP 04510, Mexico"},{"name":"Unidad Perif\u00e9rica de Investigaci\u00f3n B\u00e1sica y Cl\u00ednica en Enfermedades Infecciosas, Divisi\u00f3n de Investigaci\u00f3n de la Facultad de Medicina, UNAM: Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato-Oncolog\u00eda e Investigaci\u00f3n, Hospital Infantil de M\u00e9xico Federico G\u00f3mez\/Facultad de Medicina, UNAM. Dr. M\u00e1rquez 162, Col. Doctores, M\u00e9xico City CP 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Eslava-Campos","sequence":"additional","affiliation":[{"name":"Departamento de Salud P\u00fablica, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City CP 04510, Mexico"},{"name":"Unidad Perif\u00e9rica de Investigaci\u00f3n B\u00e1sica y Cl\u00ednica en Enfermedades Infecciosas, Divisi\u00f3n de Investigaci\u00f3n de la Facultad de Medicina, UNAM: Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato-Oncolog\u00eda e Investigaci\u00f3n, Hospital Infantil de M\u00e9xico Federico G\u00f3mez\/Facultad de Medicina, UNAM. Dr. M\u00e1rquez 162, Col. Doctores, M\u00e9xico City CP 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/S0140-6736(05)71877-8","article-title":"WHO estimates of the causes of death in children","volume":"365","author":"Bryce","year":"2005","journal-title":"Lancet"},{"key":"ref_2","unstructured":"GBD Diarrhoeal Diseases Collaborators (2017). Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: A systematic analysis for the Global Burden of Disease Study 2015. Lancet Infect. 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