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Despite causing an extraintestinal infection, previous studies showed that it did not have the usual characteristics of an extraintestinal pathogenic E. coli. Instead, it belonged to phylogenetic group B1 and harbored few known virulence genes. To evaluate the pathogenic potential of strain EC121, an extensive genome sequencing and in vitro characterization of various pathogenicity-associated properties were performed. The genomic analysis showed that strain EC121 harbors more than 50 complete virulence genetic clusters. It also displays the capacity to adhere to a variety of epithelial cell lineages and invade T24 bladder cells, as well as the ability to form biofilms on abiotic surfaces, and survive the bactericidal serum complement activity. Additionally, EC121 was shown to be virulent in the Galleria mellonella model. Furthermore, EC121 is an MDR strain harboring 14 antimicrobial resistance genes, including blaCTX-M-2. Completing the scenario, it belongs to serotype O154:H25 and to sequence type 101-B1, which has been epidemiologically linked to extraintestinal infections as well as to antimicrobial resistance spread. This study with E. coli strain EC121 shows that clinical isolates considered opportunistic might be true pathogens that go underestimated.<\/jats:p>","DOI":"10.3390\/microorganisms8060827","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T11:49:21Z","timestamp":1591012161000},"page":"827","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Virulence Potential of a Multidrug-Resistant Escherichia coli Strain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0833-4585","authenticated-orcid":false,"given":"Ana Carolina M.","family":"Santos","sequence":"first","affiliation":[{"name":"Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia\u2014DMIP, Universidade Federal de S\u00e3o Paulo\u2014Escola Paulista de Medicina (UNIFESP-EPM), S\u00e3o Paulo\u2014SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa M.","family":"Silva","sequence":"additional","affiliation":[{"name":"Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia\u2014DMIP, Universidade Federal de S\u00e3o Paulo\u2014Escola Paulista de Medicina (UNIFESP-EPM), S\u00e3o Paulo\u2014SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago B.","family":"Valiatti","sequence":"additional","affiliation":[{"name":"Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia\u2014DMIP, Universidade Federal de S\u00e3o Paulo\u2014Escola Paulista de Medicina (UNIFESP-EPM), S\u00e3o Paulo\u2014SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda F.","family":"Santos","sequence":"additional","affiliation":[{"name":"Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia\u2014DMIP, Universidade Federal de S\u00e3o Paulo\u2014Escola Paulista de Medicina (UNIFESP-EPM), S\u00e3o Paulo\u2014SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6407-5947","authenticated-orcid":false,"given":"Jos\u00e9 F.","family":"Santos-Neto","sequence":"additional","affiliation":[{"name":"Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia\u2014DMIP, Universidade Federal de S\u00e3o Paulo\u2014Escola Paulista de Medicina (UNIFESP-EPM), S\u00e3o Paulo\u2014SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0709-6282","authenticated-orcid":false,"given":"Rodrigo","family":"Cay\u00f4","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia e Bacteriologia\u2014LIB, Setor de Biologia Molecular, Microbiologia e Imunologia, Departamento de Ci\u00eancias Biol\u00f3gicas\u2014CDB, Instituto de Ci\u00eancias Ambientais, Qu\u00edmicas e Farmac\u00eauticas\u2014ICAQF, Universidade Federal de S\u00e3o Paulo\u2014UNIFESP, Diadema\u2014SP 09972-270, Brazil"},{"name":"Laborat\u00f3rio Alerta, Disciplina de Infectologia, Departamento de Medicina, Universidade Federal de S\u00e3o Paulo\u2014Escola Paulista de Medicina\u2014(UNIFESP-EPM), S\u00e3o Paulo\u2014SP 04039-032, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana P.","family":"Streling","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina de Infectologia, Departamento de Medicina, Universidade Federal de S\u00e3o Paulo\u2014Escola Paulista de Medicina\u2014(UNIFESP-EPM), S\u00e3o Paulo\u2014SP 04039-032, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina S.","family":"Nodari","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina de Infectologia, Departamento de Medicina, Universidade Federal de 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