{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:44:21Z","timestamp":1792025061026,"version":"4.4.0"},"reference-count":25,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,13]],"date-time":"2020-06-13T00:00:00Z","timestamp":1592006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BBS\/E\/I\/00007036 and BBS\/E\/I\/00007037"],"award-info":[{"award-number":["BBS\/E\/I\/00007036 and BBS\/E\/I\/00007037"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>In 2011, Bluetongue virus serotype 14 (BTV-14) was detected in Russia during routine surveillance, and was subsequently found in a number of European countries. The strain had high sequence similarity to a BTV-14 vaccine strain. We aimed to determine the risk of this BTV-14 strain causing disease in a UK sheep breed. Four Poll Dorset sheep were infected with a Polish isolate of BTV-14 and infection kinetics were monitored over 28 days. BTV RNA was detected in EDTA blood by 4 days post-infection (dpi) and remained detectable at 28 days post-infection (dpi). Peak viraemia occurred at 6 and 7 dpi with Ct values ranging between 24.6 and 27.3 in all infected animals. BTV antibodies were detected by 10 dpi using a commercial ELISA and neutralising antibodies were detected from 10 dpi. BTV was isolated between 6 and 12 dpi. All infected sheep developed mild clinical signs such as reddening of conjunctiva and mucosal membranes, with one sheep demonstrating more overt clinical signs. Two uninoculated control animals remained clinically healthy and did not have detectable BTV RNA or antibodies. The overall mild clinical symptoms caused by this BTV-14 in this highly susceptible sheep breed were in accordance with the asymptomatic infections observed in the affected countries.<\/jats:p>","DOI":"10.3390\/microorganisms8060892","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T03:17:32Z","timestamp":1592191052000},"page":"892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["BTV-14 Infection in Sheep Elicits Viraemia with Mild Clinical Symptoms"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5012-2829","authenticated-orcid":false,"given":"John","family":"Flannery","sequence":"first","affiliation":[{"name":"Non-Vesicular Reference Laboratories, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorraine","family":"Frost","sequence":"additional","affiliation":[{"name":"Non-Vesicular Reference Laboratories, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5808-4284","authenticated-orcid":false,"given":"Petra","family":"Fay","sequence":"additional","affiliation":[{"name":"Non-Vesicular Reference Laboratories, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayley","family":"Hicks","sequence":"additional","affiliation":[{"name":"Non-Vesicular Reference Laboratories, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Henstock","sequence":"additional","affiliation":[{"name":"Non-Vesicular Reference Laboratories, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5957-846X","authenticated-orcid":false,"given":"Marcin","family":"Smreczak","sequence":"additional","affiliation":[{"name":"Department of Virology, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5781-115X","authenticated-orcid":false,"given":"Anna","family":"Or\u0142owska","sequence":"additional","affiliation":[{"name":"Department of Virology, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Rajko-Nenow","sequence":"additional","affiliation":[{"name":"Non-Vesicular Reference Laboratories, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Darpel","sequence":"additional","affiliation":[{"name":"Non-Vesicular Reference Laboratories, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carrie","family":"Batten","sequence":"additional","affiliation":[{"name":"Non-Vesicular Reference Laboratories, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.antiviral.2013.07.020","article-title":"Culicoides biting midges, arboviruses and public health in Europe","volume":"100","author":"Carpenter","year":"2013","journal-title":"Antivir. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1051\/vetres:2008023","article-title":"Bluetongue virus: Virology, pathogenesis and immunity","volume":"39","author":"Mertens","year":"2008","journal-title":"Vet. Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Mellor, P.S., Baylis, M., and Mertens, P.P.C. (2009). Bluetongue virus in the mammalian host and the induced immune response. Bluetongue, Academic Press. [1st ed.].","DOI":"10.1016\/B978-012369368-6.50018-6"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e01539-19","DOI":"10.1128\/MRA.01539-19","article-title":"Complete genome sequence of a novel Bluetongue virus isolated from a commercial sheeppox vaccine","volume":"9","author":"Golender","year":"2020","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.3201\/eid1412.080818","article-title":"Genetic characterization of toggenburg Orbivirus, a new Bluetongue virus, from goats, Switzerland","volume":"14","author":"Hofmann","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.meegid.2017.03.021","article-title":"Novel putative Bluetongue virus in healthy goats from Sardinia, Italy","volume":"51","author":"Savini","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/bs.aivir.2017.08.003","article-title":"Bluetongue virus: From BTV-1 to BTV-27","volume":"99","author":"Belbis","year":"2017","journal-title":"Adv. Virus Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1111\/tbed.12453","article-title":"Re-Emergence of Bluetongue virus Serotype 8 in France, 2015","volume":"64","author":"Sailleau","year":"2017","journal-title":"Transbound Emerg. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1111\/tbed.13131","article-title":"Evidence of reduced viremia, pathogenicity and vector competence in a re-emerging European strain of Bluetongue virus serotype 8 in sheep","volume":"66","author":"Flannery","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1080\/1040841X.2016.1186005","article-title":"Current and next-generation bluetongue vaccines: Requirements, strategies, and prospects for different field situations","volume":"43","author":"Feenstra","year":"2017","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_11","first-page":"4957","article-title":"Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation(EU) No2016\/429): Bluetongue","volume":"15","author":"More","year":"2017","journal-title":"EFSA J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/j.1865-1682.2009.01092.x","article-title":"Emergence of Bluetongue Serotypes in Europe, Part 2: The Occurrence of a BTV-11 Strain in Belgium","volume":"56","author":"Mertens","year":"2009","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/tbed.12004","article-title":"Bluetongue Serotype 2 and 9 Modified Live Vaccine Viruses as Causative Agents of Abortion in Livestock: A Retrospective Analysis in Italy","volume":"61","author":"Savini","year":"2014","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1016\/j.vaccine.2009.10.107","article-title":"Viraemia and clinical disease in Dorset Poll sheep following vaccination with live attenuated Bluetongue virus vaccines serotypes 16 and 4","volume":"28","author":"Veronesi","year":"2010","journal-title":"Vaccine"},{"key":"ref_15","first-page":"15","article-title":"Prospects of next-generation vaccines for bluetongue","volume":"6","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8","DOI":"10.3389\/fvets.2020.00026","article-title":"Identification and characterization of Bluetongue virus serotype 14 in Russia","volume":"7","author":"Koltsov","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1515\/jvetres-2017-0065","article-title":"Diagnostic reliability of different RT-PCR protocols for the detection of Bluetongue virus serotype 14 (BTV-14)","volume":"61","author":"Orlowska","year":"2017","journal-title":"J. Vet. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1007\/s00705-016-2857-0","article-title":"First detection of Bluetongue virus serotype 14 in Poland","volume":"161","author":"Orlowska","year":"2016","journal-title":"Arch. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Nomikou, K., Hughes, J., Wash, R., Kellam, P., Breard, E., Zientara, S., Palmarini, M., Biek, R., and Mertens, P. (2015). Widespread Reassortment Shapes the Evolution and Epidemiology of Bluetongue virus following European Invasion. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1005056"},{"key":"ref_20","unstructured":"OIE (2012). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, OIE."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1024\/0036-7281.150.2.49","article-title":"Blauzungenkrankheit erreicht die Schweiz","volume":"150","author":"Hofmann","year":"2008","journal-title":"Schweiz. Arch. Tierheilkd."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e243","DOI":"10.1111\/tbed.12746","article-title":"Experimental infection of sheep, goats and cattle with a Bluetongue virus serotype 4 field strain from Bulgaria, 2014","volume":"65","author":"Schulz","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3726","DOI":"10.1016\/j.vaccine.2013.05.087","article-title":"Transplacental transmission of BTV-8 in sheep: BTV viraemia, antibody responses and vaccine efficacy in lambs infected in utero","volume":"31","author":"Smit","year":"2013","journal-title":"Vaccine"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.vetmic.2018.03.001","article-title":"Evaluating the most appropriate pooling ratio for EDTA blood samples to detect Bluetongue virus using real-time RT-PCR","volume":"217","author":"Flannery","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1136\/vr.161.8.253","article-title":"Clinical signs and pathology shown by British sheep and cattle infected with Bluetongue virus serotype 8 derived from the 2006 outbreak in northern Europe","volume":"161","author":"Darpel","year":"2007","journal-title":"Vet. Rec."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/6\/892\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:38:35Z","timestamp":1760175515000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/6\/892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,13]]},"references-count":25,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["microorganisms8060892"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms8060892","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,13]]}}}