{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:28:38Z","timestamp":1765232918445,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,26]],"date-time":"2020-06-26T00:00:00Z","timestamp":1593129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"20091798, 20101275, 20113149,2007\/C01\/71285, HIM\/2008\/031 and HIM\/2019\/018","award":["2007\/C01\/71285"],"award-info":[{"award-number":["2007\/C01\/71285"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>NOTCH1 and PAX5 participate in the proliferation and differentiation of B and T lymphocytes. Their expression can be modified by activation of NOTCH1, induced by the Epstein\u2013Barr (EBV) viral proteins identified as LMP1 and LMP2. To identify whether PAX5, NOTCH1, and EBV latency genes participate in the oncogenic process of pediatric patients with classical Hodgkin lymphoma (cHL), the present study aimed to identify the variable expression of NOTCH1 among disease subtypes and to assess its effect on PAX5 expression. A total of 41 paraffin-embedded tissues from Mexican pediatric patients with cHL were analyzed. The expression of CD30, CD20, NOTCH1, PAX5, and LMP1 was evaluated by immunohistochemistry and immunofluorescence. EBV detection was performed by in situ hybridization. Out of all cases, 78% (32\/41) of the cHL cases were EBV positive. NOTCH1 expression was detected in 78.1% (25\/32) of EBV-positive cases, nodular sclerosis being the most frequent subtype (11\/25, 44%). In cases where the expression of both genes was identified, double immunofluorescence assays were conducted, finding no colocalization. We found that Reed\u2013Sternberg cells had aberrant expression compared to their cells of origin (B lymphocytes) due to the molecular mechanisms involved in the loss of expression of PAX5 and that the identification of NOTCH1 could be considered as a candidate diagnostic\/prognostic marker and a therapeutic target in pediatric cHL.<\/jats:p>","DOI":"10.3390\/microorganisms8060958","type":"journal-article","created":{"date-parts":[[2020,6,26]],"date-time":"2020-06-26T05:19:36Z","timestamp":1593148776000},"page":"958","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Variable Expression of Notch1 and Pax5 in Classical Hodgkin Lymphoma and Infection with Epstein\u2013Barr in Pediatric Patients"],"prefix":"10.3390","volume":"8","author":[{"given":"Icela","family":"Palma-Lara","sequence":"first","affiliation":[{"name":"Molecular and Cellular Morphology Laboratory, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Ciudad de Mexico 11340, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Elena","family":"S\u00e1nchez-Aldana","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Morphology Laboratory, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Ciudad de Mexico 11340, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elva","family":"Jim\u00e9nez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Hematolog\u00eda Pedi\u00e1trica, Centro M\u00e9dico Nacional la Raza, Instituto Mexicano del Seguro Social, Ciudad de Mexico 02990, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8881-1104","authenticated-orcid":false,"given":"Octavio","family":"Mart\u00ednez-Villegas","sequence":"additional","affiliation":[{"name":"Departamento de Hematolog\u00eda Pedi\u00e1trica, Centro M\u00e9dico Nacional la Raza, Instituto Mexicano del Seguro Social, Ciudad de Mexico 02990, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8070-9727","authenticated-orcid":false,"given":"Juan Carlos","family":"N\u00fa\u00f1ez-Enr\u00edquez","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Epidemiolog\u00eda Cl\u00ednica, Unidad M\u00e9dica de Alta Especialidad Hospital de Pediatr\u00eda \u201cDr., Silvestre Frenk Freud\u201d Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de Mexico 06600, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8027-6231","authenticated-orcid":false,"given":"Juan Manuel","family":"Mej\u00eda-Arangur\u00e9","sequence":"additional","affiliation":[{"name":"Coordinaci\u00f3n de Investigaci\u00f3n en Salud, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de Mexico 06600, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara A.","family":"Ochoa","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Bacteriolog\u00eda Intestinal, Hospital Infantil de Mexico Federico G\u00f3mez, Ciudad de Mexico 06720, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Xicohtencatl-Cortes","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Bacteriolog\u00eda Intestinal, Hospital Infantil de Mexico Federico G\u00f3mez, Ciudad de Mexico 06720, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariadnna","family":"Cruz-C\u00f3rdova","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Bacteriolog\u00eda Intestinal, Hospital Infantil de Mexico Federico G\u00f3mez, Ciudad de Mexico 06720, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Zavala-Vega","sequence":"additional","affiliation":[{"name":"Laboratorio de Neuropatolog\u00eda, Instituto Nacional de neurolog\u00eda Manuel Velasco Su\u00e1rez, Ciudad de Mexico 14269, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana","family":"Garc\u00eda-Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Facultad de Estudios Superiores Iztacala, Universidad Nacional Aut\u00f3noma de Mexico, Ciudad de Mexico 54090, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5173-9927","authenticated-orcid":false,"given":"Alejandra","family":"Contreras-Ramos","sequence":"additional","affiliation":[{"name":"Biology Development Department, Hospital Infantil de Mexico Federico G\u00f3mez, Ciudad de Mexico 06720, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Refugio","family":"Torres-Nava","sequence":"additional","affiliation":[{"name":"Servicio de Oncohematolog\u00eda, Hospital Pedi\u00e1trico Moctezuma, Secretar\u00eda de Salud del Gobierno de la Ciudad de Mexico 15530, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo","family":"Mora-Ramiro","sequence":"additional","affiliation":[{"name":"Laboratorio de Virolog\u00eda Cl\u00ednica y Experimental, Unidad de Investigaci\u00f3n en Enfermedades Infecciosas, Hospital Infantil de Mexico Federico G\u00f3mez, Ciudad de Mexico 06720, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Arellano-Galindo","sequence":"additional","affiliation":[{"name":"Laboratorio de Virolog\u00eda Cl\u00ednica y Experimental, Unidad de Investigaci\u00f3n en Enfermedades Infecciosas, Hospital Infantil de Mexico Federico G\u00f3mez, Ciudad de Mexico 06720, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,26]]},"reference":[{"key":"ref_1","first-page":"e96590","article-title":"Prevalence of Germ Cell Tumors in a Mexican Tertiary Hospital and the Creation of a Probabilistic Network of Risk Factors","volume":"30","author":"Montes","year":"2020","journal-title":"Iran. 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