{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T21:25:04Z","timestamp":1785014704676,"version":"3.55.0"},"reference-count":62,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T00:00:00Z","timestamp":1594598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U19AI062629"],"award-info":[{"award-number":["U19AI062629"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM121601"],"award-info":[{"award-number":["GM121601"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Neutrophils are the most abundant innate cell population and a key immune player against invading pathogens. Neutrophils can kill both bacterium and spores of Bacillus anthracis, the causative anthrax pathogen. Unlike interactions with professional phagocytes, the molecular recognition of anthrax by neutrophils is largely unknown. In this study, we investigated the role of complement C3 deposition on anthrax particles for neutrophil recognition of bacterium and\/or its cell wall peptidoglycan, an abundant pathogen-associated molecular pattern that supports anthrax sepsis. C3 opsonization and recognition by complement receptors accounted for 70\u201380% of the affinity interactions between neutrophils and anthrax particles at subphysiologic temperatures. In contrast, C3 supported up to 50% of the anthrax particle ingestion under thermophysiologic conditions. Opsonin-dependent low affinity interactions and, to a lower extent, opsonin-independent mechanisms, provide alternative entry routes. Similarly, C3 supported 58% of peptidoglycan-induced degranulation and, to a lower extent, 23% of bacterium-induced degranulation. Interestingly, an opsonin independent mechanism mediated by complement C5, likely through C5a anaphylatoxin, primes azurophilic granules in response to anthrax particles. Overall, we show that C3 deposition supports anthrax recognition by neutrophils but is dispensable for pathogen ingestion and neutrophil degranulation, highlighting immune recognition redundancies that minimize the risk of pathogen evasion.<\/jats:p>","DOI":"10.3390\/microorganisms8071039","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T04:46:01Z","timestamp":1594701961000},"page":"1039","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["C3 Opsonization of Anthrax Bacterium and Peptidoglycan Supports Recognition and Activation of Neutrophils"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4809-9885","authenticated-orcid":false,"given":"Narcis I.","family":"Popescu","sequence":"first","affiliation":[{"name":"Department of Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi S.","family":"Keshari","sequence":"additional","affiliation":[{"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackie","family":"Cochran","sequence":"additional","affiliation":[{"name":"Department of Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Mark","family":"Coggeshall","sequence":"additional","affiliation":[{"name":"Department of Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1249-9278","authenticated-orcid":false,"given":"Florea","family":"Lupu","sequence":"additional","affiliation":[{"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1164\/rccm.201102-0209CI","article-title":"Anthrax infection","volume":"184","author":"Sweeney","year":"2011","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1146\/annurev-micro-091014-104523","article-title":"Anthrax Pathogenesis","volume":"69","author":"Moayeri","year":"2015","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1038\/s41564-019-0435-4","article-title":"The global distribution of Bacillus anthracis and associated anthrax risk to humans, livestock and wildlife","volume":"4","author":"Carlson","year":"2019","journal-title":"Nat. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"9269","DOI":"10.1038\/s41598-018-27604-w","article-title":"Climatic influence on anthrax suitability in warming northern latitudes","volume":"8","author":"Walsh","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.1001\/jama.287.17.2236","article-title":"Anthrax as a biological weapon, 2002: updated recommendations for management","volume":"287","author":"Inglesby","year":"2002","journal-title":"JAMA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1126\/science.7973702","article-title":"The Sverdlovsk anthrax outbreak of 1979","volume":"266","author":"Meselson","year":"1994","journal-title":"Science"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"933","DOI":"10.3201\/eid0706.010604","article-title":"Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States","volume":"7","author":"Jernigan","year":"2001","journal-title":"Emerg. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1111\/jcmm.12075","article-title":"The sepsis model: an emerging hypothesis for the lethality of inhalation anthrax","volume":"17","author":"Coggeshall","year":"2013","journal-title":"J. Cell Mol. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.3389\/fmicb.2015.01122","article-title":"Crossing of the epithelial barriers by Bacillus anthracis: the Known and the Unknown","volume":"6","author":"Goossens","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.micpath.2009.03.004","article-title":"Complement protein C3 binding to Bacillus anthracis spores enhances phagocytosis by human macrophages","volume":"46","author":"Premanandan","year":"2009","journal-title":"Microb. Pathog."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4421","DOI":"10.4049\/jimmunol.1102092","article-title":"Activation of the classical complement pathway by Bacillus anthracis is the primary mechanism for spore phagocytosis and involves the spore surface protein BclA","volume":"188","author":"Gu","year":"2012","journal-title":"J. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1073\/pnas.0709321105","article-title":"The integrin Mac-1 (CR3) mediates internalization and directs Bacillus anthracis spores into professional phagocytes","volume":"105","author":"Oliva","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1046\/j.1462-5822.2000.00067.x","article-title":"Early Bacillus anthracis-macrophage interactions: intracellular survival survival and escape","volume":"2","author":"Dixon","year":"2000","journal-title":"Cell Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.micpath.2010.02.004","article-title":"Transport of Bacillus anthracis from the lungs to the draining lymph nodes is a rapid process facilitated by CD11c+ cells","volume":"49","author":"Huffnagle","year":"2010","journal-title":"Microb. Pathog."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.mam.2009.07.003","article-title":"Cellular and systemic effects of anthrax lethal toxin and edema toxin","volume":"30","author":"Moayeri","year":"2009","journal-title":"Mol. Aspects Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1111\/j.1365-2958.2006.05179.x","article-title":"Poly-gamma-glutamate in bacteria","volume":"60","author":"Candela","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s15010-003-3169-3","article-title":"Cutaneous anthrax in adults: a review of 25 cases in the eastern Anatolian region of Turkey","volume":"31","author":"Demirdag","year":"2003","journal-title":"Infection"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1111\/j.1365-2613.2008.00584.x","article-title":"Superficial exudates of neutrophils prevent invasion of Bacillus anthracis bacilli into abraded skin of resistant mice","volume":"89","author":"Hahn","year":"2008","journal-title":"Int, J. Exp. Pathol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1128\/IAI.74.1.469-480.2006","article-title":"Roles of macrophages and neutrophils in the early host response to Bacillus anthracis spores in a mouse model of infection","volume":"74","author":"Cote","year":"2006","journal-title":"Infect. Immun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3389\/fcimb.2018.00002","article-title":"Innate Immune Interactions between Bacillus anthracis and Host Neutrophils","volume":"8","author":"Liu","year":"2018","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Moayeri, M., Crown, D., Newman, Z.L., Okugawa, S., Eckhaus, M., Cataisson, C., Liu, S., Sastalla, I., and Leppla, S.H. (2010). Inflammasome sensor Nlrp1b-dependent resistance to anthrax is mediated by caspase-1, IL-1 signaling and neutrophil recruitment. PLoS Pathog., 6.","DOI":"10.1371\/journal.ppat.1001222"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"433","DOI":"10.2353\/ajpath.2006.051330","article-title":"Sepsis and pathophysiology of anthrax in a nonhuman primate model","volume":"169","author":"Lupu","year":"2006","journal-title":"Am. J. Pathol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Mayer-Scholl, A., Hurwitz, R., Brinkmann, V., Schmid, M., Jungblut, P., Weinrauch, Y., and Zychlinsky, A. (2005). Human neutrophils kill Bacillus anthracis. PLoS Pathog., 1.","DOI":"10.1371\/journal.ppat.0010023"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/modpathol.3880337","article-title":"Quantitative pathology of inhalational anthrax I: quantitative microscopic findings","volume":"14","author":"Grinberg","year":"2001","journal-title":"Mod. Pathol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1128\/IAI.00985-07","article-title":"Detection of Bacillus anthracis spore germination in vivo by bioluminescence imaging","volume":"76","author":"Sanz","year":"2008","journal-title":"Infect. Immun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1128\/iai.47.1.306-310.1985","article-title":"Effects of anthrax toxin components on human neutrophils","volume":"47","author":"Friedlander","year":"1985","journal-title":"Infect. Immun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7557","DOI":"10.4049\/jimmunol.176.12.7557","article-title":"Bacillus anthracis toxins inhibit human neutrophil NADPH oxidase activity","volume":"176","author":"Crawford","year":"2006","journal-title":"J. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1111\/cmi.12232","article-title":"Circulating lethal toxin decreases the ability of neutrophils to respond to Bacillus anthracis","volume":"16","author":"Weiner","year":"2014","journal-title":"Cell Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1958","DOI":"10.3389\/fimmu.2019.01958","article-title":"Expression, Role, and Regulation of Neutrophil Fcgamma Receptors","volume":"10","author":"Wang","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/j.molimm.2004.06.011","article-title":"Complement: a unique innate immune sensor for danger signals","volume":"41","author":"Gasque","year":"2004","journal-title":"Mol. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.semcdb.2017.11.025","article-title":"Non-identical twins: Different faces of CR3 and CR4 in myeloid and lymphoid cells of mice and men","volume":"85","author":"Erdei","year":"2019","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1182\/blood-2003-04-1078","article-title":"Toll-like receptors stimulate human neutrophil function","volume":"102","author":"Hayashi","year":"2003","journal-title":"Blood"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1182\/blood-2017-10-813618","article-title":"Peptidoglycan induces disseminated intravascular coagulation in baboons through activation of both coagulation pathways","volume":"132","author":"Popescu","year":"2018","journal-title":"Blood"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1093\/infdis\/jit247","article-title":"Bacillus anthracis cell wall peptidoglycan but not lethal or edema toxins produces changes consistent with disseminated intravascular coagulation in a rat model","volume":"208","author":"Qiu","year":"2013","journal-title":"J. Infect. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2436","DOI":"10.1182\/bloodadvances.2019000513","article-title":"Monocyte procoagulant responses to anthrax peptidoglycan are reinforced by proinflammatory cytokine signaling","volume":"3","author":"Popescu","year":"2019","journal-title":"Blood Adv."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2418","DOI":"10.1128\/IAI.00170-10","article-title":"Inflammatory cytokine response to Bacillus anthracis peptidoglycan requires phagocytosis and lysosomal trafficking","volume":"78","author":"Iyer","year":"2010","journal-title":"Infect. Immun."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Langer, M., Girton, A.W., Popescu, N.I., Burgett, T., Metcalf, J.P., and Coggeshall, K.M. (2018). Neither Lys- and DAP-type peptidoglycans stimulate mouse or human innate immune cells via Toll-like receptor 2. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0193207"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Langer, M., Malykhin, A., Maeda, K., Chakrabarty, K., Williamson, K.S., Feasley, C.L., West, C.M., Metcalf, J.P., and Coggeshall, K.M. (2008). Bacillus anthracis peptidoglycan stimulates an inflammatory response in monocytes through the p38 mitogen-activated protein kinase pathway. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0003706"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e00076-18","DOI":"10.1128\/IAI.00076-18","article-title":"Serum Amyloid P and IgG Exhibit Differential Capabilities in the Activation of the Innate Immune System in Response to Bacillus anthracis Peptidoglycan","volume":"86","author":"Girton","year":"2018","journal-title":"Infect. Immun."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Pulli, B., Ali, M., Forghani, R., Schob, S., Hsieh, K.L., Wojtkiewicz, G., Linnoila, J.J., and Chen, J.W. (2013). Measuring myeloperoxidase activity in biological samples. PLoS One, 8.","DOI":"10.1371\/journal.pone.0067976"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"29241","DOI":"10.1074\/jbc.M704587200","article-title":"Structure of compstatin in complex with complement component C3c reveals a new mechanism of complement inhibition","volume":"282","author":"Janssen","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1182\/blood-2013-02-486613","article-title":"Bacillus anthracis peptidoglycan activates human platelets through FcgammaRII and complement","volume":"122","author":"Sun","year":"2013","journal-title":"Blood"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3503","DOI":"10.1182\/blood.V89.10.3503","article-title":"Granules of the human neutrophilic polymorphonuclear leukocyte","volume":"89","author":"Borregaard","year":"1997","journal-title":"Blood"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3007","DOI":"10.1182\/blood.V92.9.3007","article-title":"Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing","volume":"92","author":"Hampton","year":"1998","journal-title":"Blood"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1189\/jlb.0711387","article-title":"Myeloperoxidase associated with neutrophil extracellular traps is active and mediates bacterial killing in the presence of hydrogen peroxide","volume":"91","author":"Parker","year":"2012","journal-title":"J. Leukoc. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"7406","DOI":"10.4049\/jimmunol.173.12.7406","article-title":"TLRs govern neutrophil activity in aspergillosis","volume":"173","author":"Bellocchio","year":"2004","journal-title":"J. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7994","DOI":"10.4049\/jimmunol.178.12.7994","article-title":"Lung dendritic cells rapidly mediate anthrax spore entry through the pulmonary route","volume":"178","author":"Cleret","year":"2007","journal-title":"J. Immunol."},{"key":"ref_48","first-page":"624","article-title":"Role of neutrophil Fc gamma RIIa (CD32) and Fc gamma RIIIb (CD16) polymorphic forms in phagocytosis of human IgG1- and IgG3-opsonized bacteria and erythrocytes","volume":"83","author":"Bredius","year":"1994","journal-title":"Immunology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"330","DOI":"10.4049\/jimmunol.151.1.330","article-title":"Signaling properties of CR3 (CD11b\/CD18) and CR1 (CD35) in relation to phagocytosis of complement-opsonized particles","volume":"151","author":"Fallman","year":"1993","journal-title":"J. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4421","DOI":"10.1182\/blood-2011-12-401133","article-title":"Endocytosis of soluble immune complexes leads to their clearance by FcgammaRIIIB but induces neutrophil extracellular traps via FcgammaRIIA in vivo","volume":"120","author":"Chen","year":"2012","journal-title":"Blood"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1084\/jem.20161512","article-title":"TLR7\/8 activation in neutrophils impairs immune complex phagocytosis through shedding of FcgRIIA","volume":"214","author":"Lood","year":"2017","journal-title":"J. Exp. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.4049\/jimmunol.1201302","article-title":"Anti-peptidoglycan antibodies and Fcgamma receptors are the key mediators of inflammation in Gram-positive sepsis","volume":"189","author":"Sun","year":"2012","journal-title":"J. Immunol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/S0092-8674(02)00672-4","article-title":"Structure of the C3b binding site of CR1 (CD35), the immune adherence receptor","volume":"108","author":"Smith","year":"2002","journal-title":"Cell"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Jensen, R.K., Bajic, G., Sen, M., Springer, T.A., Vorup-Jensen, T., and Andersen, G.R. (2020). Complement receptor 3 forms a compact high affinity complex with iC3b. BioRxiv.","DOI":"10.1101\/2020.04.15.043133"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"44898","DOI":"10.1074\/jbc.M106819200","article-title":"The human low affinity Fcgamma receptors IIa, IIb, and III bind IgG with fast kinetics and distinct thermodynamic properties","volume":"276","author":"Maenaka","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1172\/JCI113643","article-title":"Neutrophil and monocyte cell surface p150,95 has iC3b-receptor (CR4) activity resembling CR3","volume":"82","author":"Myones","year":"1988","journal-title":"J. Clin. Invest."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/S1074-7613(00)80356-8","article-title":"Complement receptor type 1 (CR1, CD35) is a receptor for C1q","volume":"7","author":"Klickstein","year":"1997","journal-title":"Immunity"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"915","DOI":"10.4161\/mabs.28808","article-title":"Highly parallel characterization of IgG Fc binding interactions","volume":"6","author":"Boesch","year":"2014","journal-title":"MAbs"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3841","DOI":"10.4049\/jimmunol.1004058","article-title":"Cutting edge: primary innate immune cells respond efficiently to polymeric peptidoglycan, but not to peptidoglycan monomers","volume":"186","author":"Iyer","year":"2011","journal-title":"J. Immunol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1172\/JCI118777","article-title":"Soluble beta-glucan polysaccharide binding to the lectin site of neutrophil or natural killer cell complement receptor type 3 (CD11b\/CD18) generates a primed state of the receptor capable of mediating cytotoxicity of iC3b-opsonized target cells","volume":"98","author":"Vetvicka","year":"1996","journal-title":"J. Clin. Invest."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.4049\/jimmunol.156.3.1235","article-title":"Analysis of the sugar specificity and molecular location of the beta-glucan-binding lectin site of complement receptor type 3 (CD11b\/CD18)","volume":"156","author":"Thornton","year":"1996","journal-title":"J. Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1182\/blood-2010-06-290171","article-title":"Myeloperoxidase is required for neutrophil extracellular trap formation: implications for innate immunity","volume":"117","author":"Metzler","year":"2011","journal-title":"Blood"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/7\/1039\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:50:55Z","timestamp":1760176255000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/7\/1039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,13]]},"references-count":62,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["microorganisms8071039"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms8071039","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,13]]}}}