{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T17:53:20Z","timestamp":1778003600000,"version":"3.51.4"},"reference-count":36,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T00:00:00Z","timestamp":1594771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Numerous Salmonella enterica serovars can cause disease and contamination of animal-produced foods. Oligosaccharide-rich products capable of blocking pathogen adherence to intestinal mucosa are attractive alternatives to antibiotics as these have potential to prevent enteric infections. Presently, a wood-derived prebiotic composed mainly of glucose-galactose-mannose-xylose oligomers was found to inhibit mannose-sensitive binding of select Salmonella Typhimurium and Escherichia coli strains when reacted with Saccharomyces boulardii. Tests for the ability of the prebiotic to prevent binding of a green fluorescent protein (GFP)-labeled S. Typhimurium to intestinal porcine epithelial cells (IPEC-J2) cultured in vitro revealed that prebiotic-exposed GFP-labeled S. Typhimurium bound &gt; 30% fewer individual IPEC-J2 cells than did GFP-labeled S. Typhimurium having no prebiotic exposure. Quantitatively, 90% fewer prebiotic-exposed GFP-labeled S. Typhimurium cells were bound per individual IPEC-J2 cell compared to non-prebiotic exposed GFP-labeled S. Typhimurium. Comparison of invasiveness of S. Typhimurium DT104 against IPEC-J2 cells revealed greater than a 90% decrease in intracellular recovery of prebiotic-exposed S. Typhimurium DT104 compared to non-exposed controls (averaging 4.4 \u00b1 0.2 log10 CFU\/well). These results suggest compounds within the wood-derived prebiotic bound to E. coli and S. Typhimurium-produced adhesions and in the case of S. Typhimurium, this adhesion-binding activity inhibited the binding and invasion of IPEC-J2 cells.<\/jats:p>","DOI":"10.3390\/microorganisms8071051","type":"journal-article","created":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T10:54:46Z","timestamp":1594896886000},"page":"1051","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Inhibition of Salmonella Binding to Porcine Intestinal Cells by a Wood-Derived Prebiotic"],"prefix":"10.3390","volume":"8","author":[{"given":"Aleksandar","family":"Bo\u017ei\u0107","sequence":"first","affiliation":[{"name":"Faculty of Agriculture, University of Novi Sad, Novi Sad 21000, Serbia"},{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin C.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tawni L.","family":"Crippen","sequence":"additional","affiliation":[{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5057-8175","authenticated-orcid":false,"given":"Christina L.","family":"Swaggerty","sequence":"additional","affiliation":[{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael E.","family":"Hume","sequence":"additional","affiliation":[{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3445-1008","authenticated-orcid":false,"given":"Ross C.","family":"Beier","sequence":"additional","affiliation":[{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiqi","family":"He","sequence":"additional","affiliation":[{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth J.","family":"Genovese","sequence":"additional","affiliation":[{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toni L.","family":"Poole","sequence":"additional","affiliation":[{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger B.","family":"Harvey","sequence":"additional","affiliation":[{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Nisbet","sequence":"additional","affiliation":[{"name":"Food and Feed Safety Research Unit, United States Department of Agriculture\/Agricultural Research Service, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.3923\/ajava.2015.803.829","article-title":"A review of prevention and control methods of salmonella species in swine production and the role of dietary non-nutritional additives","volume":"10","author":"Callegari","year":"2015","journal-title":"Asian J. 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