{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T14:22:22Z","timestamp":1771078942269,"version":"3.50.1"},"reference-count":73,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T00:00:00Z","timestamp":1595462400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Vibrio cholerae non-O1, non-O139 bacteria are natural inhabitants of aquatic ecosystems and have been sporadically associated with human infections. They mostly lack the two major virulence factors of toxigenic V. cholerae serogroups O1 and O139 strains, which are the causative agent of cholera. Non-O1, non-O139 strains are found in water bodies, sediments, and in association with other aquatic organisms. Occurrence of these bacteria in fecal specimens of waterfowl were reported, and migratory birds likely contribute to the long-distance transfer of strains. We investigated four V. cholerae non-O1, non-O139 isolates for phenotypic traits and by whole genome sequencing (WGS). The isolates were recovered from organs of domestic ducks with serious disease symptoms. WGS data revealed only a distant genetic relationship between all isolates. The isolates harbored a number of virulence factors found in most V. cholerae strains. Specific virulence factors of non-O1, non-O139 strains, such as the type III secretion system (TTSS) or cholix toxin, were observed. An interesting observation is that all isolates possess multifunctional autoprocessing repeats-in-toxin toxins (MARTX) closely related to the MARTX of toxigenic El Tor O1 strains. Different primary sequences of the abundant OmpU proteins could indicate a significant role of this virulence factor. Phenotypic characteristics such as hemolysis and antimicrobial resistance (AMR) were studied. Three isolates showed susceptibility to a number of tested antimicrobials, and one strain possessed AMR genes located in an integron. Knowledge of the environmental occurrence of V. cholerae non-O1, non-O139 in Germany is limited. The source of the infection of the ducks is currently unknown. In the context of the \u2018One Health\u2019 concept, it is desirable to study the ecology of V. cholerae non-O1, non-O139, as it cannot be excluded that the isolates possess zoonotic potential and could cause infections in humans.<\/jats:p>","DOI":"10.3390\/microorganisms8081104","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T10:14:06Z","timestamp":1595499246000},"page":"1104","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Phenotypic and Genotypic Properties of Vibrio cholerae non-O1, non-O139 Isolates Recovered from Domestic Ducks in Germany"],"prefix":"10.3390","volume":"8","author":[{"given":"Nicola","family":"Hirsch","sequence":"first","affiliation":[{"name":"Tiergesundheitsdienst Bayern, Bavarian Animal Health Service, 85586 Poing, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Kappe","sequence":"additional","affiliation":[{"name":"Tiergesundheitsdienst Bayern, Bavarian Animal Health Service, 85586 Poing, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armin","family":"Gangl","sequence":"additional","affiliation":[{"name":"Tiergesundheitsdienst Bayern, Bavarian Animal Health Service, 85586 Poing, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keike","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Department of Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Mayer-Scholl","sequence":"additional","affiliation":[{"name":"Department of Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6930-4358","authenticated-orcid":false,"given":"Jens Andre","family":"Hammerl","sequence":"additional","affiliation":[{"name":"Department of Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2494-0278","authenticated-orcid":false,"given":"Eckhard","family":"Strauch","sequence":"additional","affiliation":[{"name":"Department of Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2466","DOI":"10.1016\/S0140-6736(12)60436-X","article-title":"Cholera","volume":"379","author":"Harris","year":"2012","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s10096-013-2011-9","article-title":"Genetic and phenotypic analysis of Vibrio cholerae non-O1, non-O139 isolated from German and Austrian patients","volume":"33","author":"Schirmeister","year":"2014","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis. Off. Publ. Eur. Soc. Clin. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"282","DOI":"10.3389\/fmicb.2017.00282","article-title":"Fish as Hosts of Vibrio cholerae","volume":"8","author":"Halpern","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.fsi.2019.01.050","article-title":"The pathogenicity characterization of non-O1 Vibrio cholerae and its activation on immune system in freshwater shrimp Macrobrachium nipponense","volume":"87","author":"Li","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.jip.2015.06.002","article-title":"Vibrio cholerae pathogen from the freshwater-cultured whiteleg shrimp Penaeus vannamei and control with Bdellovibrio bacteriovorus","volume":"130","author":"Cao","year":"2015","journal-title":"J. Invertebr. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1128\/aem.55.1.95-99.1989","article-title":"Isolation of Vibrio cholerae from aquatic birds in Colorado and Utah","volume":"55","author":"Ogg","year":"1989","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1111\/j.1365-2672.1982.tb04852.x","article-title":"The incidence of Vibrio cholerae in water, animals and birds in Kent, England","volume":"52","author":"Lee","year":"1982","journal-title":"J. Appl. Bacteriol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1111\/jam.13705","article-title":"A comprehensive survey of Aeromonas sp. and Vibrio sp. in seabirds from southeastern Brazil: Outcomes for public health","volume":"124","author":"Cardoso","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1111\/1462-2920.13612","article-title":"High genetic diversity of Vibrio cholerae in the European lake Neusiedler See is associated with intensive recombination in the reed habitat and the long-distance transfer of strains","volume":"19","author":"Pretzer","year":"2017","journal-title":"Environ. Microbiol."},{"key":"ref_10","first-page":"271","article-title":"Isolation, characterization and public health aspects of Vibrio cholerae NAG isolated from a Danish duck farm","volume":"4","author":"Bisgaard","year":"1975","journal-title":"Avian Pathol."},{"key":"ref_11","first-page":"261","article-title":"Prevalence of non-cholera vibrios in cavum nasi and pharynx of ducks","volume":"86","author":"Bisgaard","year":"1978","journal-title":"Acta Path. Microb. Scand. Sect. B"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"199","DOI":"10.2307\/1589842","article-title":"A non-O1 Vibrio cholerae isolated from a goose","volume":"25","author":"Schlater","year":"1981","journal-title":"Avian Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1177\/104063879300500423","article-title":"Minimum inhibitory concentrations of bacteria isolated from septicemia and airsacculitis in ducks","volume":"5","author":"Watts","year":"1993","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_14","first-page":"60","article-title":"Antibiotic resistance of Vibrio cholerae from poultry sources of Dhaka, Bangladesh","volume":"2","author":"Akond","year":"2008","journal-title":"Adv. Biolog. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1128\/JCM.01544-06","article-title":"Identification of Vibrio isolates by a multiplex PCR assay and rpoB sequence determination","volume":"45","author":"Tarr","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1128\/JCM.02026-08","article-title":"Incidence, virulence factors, and clonality among clinical strains of non-O1, non-O139 Vibrio cholerae isolates from hospitalized diarrheal patients in Kolkata, india","volume":"47","author":"Chatterjee","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"419","DOI":"10.2323\/jgam.55.419","article-title":"Molecular characterization of serogrouping and virulence genes of Malaysian Vibrio cholerae isolated from different sources","volume":"55","author":"Ahmad","year":"2009","journal-title":"J. Gen. Appl. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/j.1574-695X.1998.tb01128.x","article-title":"Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139","volume":"20","author":"Hoshino","year":"1998","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"J\u00e4ckel, C., Hammerl, J.A., Arslan, H.H., G\u00f6llner, C., Vom Ort, N., Taureck, K., and Strauch, E. (2020). Phenotypic and genotypic characterization of veterinary Vibrio cincinnatiensis isolates. Microorganisms, 8.","DOI":"10.3390\/microorganisms8050739"},{"key":"ref_20","unstructured":"CLSI (2015). Clinical and Laboratory Standards Institute. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline, 3rd Edition M45, CLSI."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"EFSA (2019). The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2017. EFSA J., 17.","DOI":"10.2903\/j.efsa.2019.5598"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"733","DOI":"10.3389\/fmicb.2019.00733","article-title":"Environmental and clinical strains of Vibrio cholerae Non-O1, Non-O139 from Germany possess similar virulence gene profiles","volume":"10","author":"Schwartz","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"D535","DOI":"10.1093\/nar\/gkw1017","article-title":"Improvements to PATRIC, the all-bacterial bioinformatics database and analysis resource center","volume":"45","author":"Wattam","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"W16","DOI":"10.1093\/nar\/gkw387","article-title":"PHASTER: A better, faster version of the PHAST phage search tool","volume":"44","author":"Arndt","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1128\/JCM.06094-11","article-title":"Multilocus sequence typing of total-genome-sequenced bacteria","volume":"50","author":"Larsen","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Octavia, S., Salim, A., Kurniawan, J., Lam, C., Leung, Q., Ahsan, S., Reeves, P.R., Nair, G.B., and Lan, R. (2013). Population structure and evolution of non-O1\/non-O139 Vibrio cholerae by multilocus sequence typing. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0065342"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/AAC.02412-14","article-title":"In silico detection and typing of plasmids using plasmidfinder and plasmid multilocus sequence typing","volume":"58","author":"Carattoli","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Kaas, R.S., Leekitcharoenphon, P., Aarestrup, F.M., and Lund, O. (2014). Solving the problem of comparing whole bacterial genomes across different sequencing platforms. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0104984"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"D694","DOI":"10.1093\/nar\/gkv1239","article-title":"VFDB 2016: Hierarchical and refined dataset for big data analysis\u201410 years on","volume":"44","author":"Chen","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3681","DOI":"10.1099\/00221287-148-11-3681","article-title":"Characterization of a novel Vibrio pathogenicity island (VPI-2) encoding neuraminidase (nanH) among toxigenic Vibrio cholerae isolates","volume":"148","author":"Jermyn","year":"2002","journal-title":"Microbiology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1073\/pnas.042667999","article-title":"Comparative genomic analysis of Vibrio cholerae: Genes that correlate with cholera endemic and pandemic disease","volume":"99","author":"Dziejman","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4053","DOI":"10.1099\/mic.0.27172-0","article-title":"The Vibrio seventh pandemic island-II is a 26.9 kb genomic island present in Vibrio cholerae El Tor and O139 serogroup isolates that shows homology to a 43.4 kb genomic island in V. vulnificus","volume":"150","author":"Finnan","year":"2004","journal-title":"Microbiology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2862","DOI":"10.1128\/IAI.03020-14","article-title":"Type 3 secretion system island encoded proteins required for colonization by Non-O1\/non-O139 serogroup Vibrio cholerae","volume":"83","author":"Chaand","year":"2015","journal-title":"Infect. Immun."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3465","DOI":"10.1073\/pnas.0409918102","article-title":"Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system","volume":"102","author":"Dziejman","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e00927-14","DOI":"10.1128\/genomeA.00927-14","article-title":"Taxonomic affiliation of new genomes should be verified using average nucleotide identity and multilocus phylogenetic analysis","volume":"2","author":"Figueras","year":"2014","journal-title":"Genome Announc."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2902","DOI":"10.1128\/JB.181.9.2902-2913.1999","article-title":"Characterization of the major control region of Vibrio cholerae bacteriophage K139: Immunity, exclusion, and integration","volume":"181","author":"Nesper","year":"1999","journal-title":"J. Bacteriol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/1348-0421.12570","article-title":"Analysis of Vibrio seventh pandemic island II and novel genomic islands in relation to attachment sequences among a wide variety of Vibrio cholerae strains","volume":"62","author":"Nguyen","year":"2018","journal-title":"Microbiol. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S1369-5274(02)00005-X","article-title":"Filamentous phages linked to virulence of Vibrio cholerae","volume":"6","author":"Davis","year":"2003","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3134","DOI":"10.1073\/pnas.95.6.3134","article-title":"A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains","volume":"95","author":"Karaolis","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1128\/JB.00562-07","article-title":"Three pathogenicity islands of Vibrio cholerae can excise from the chromosome and form circular intermediates","volume":"190","author":"Murphy","year":"2008","journal-title":"J. Bacteriol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"VE-0002","DOI":"10.1128\/microbiolspec.VE-0002-2014","article-title":"Multifunctional-Autoprocessing repeats-in-toxin (MARTX) Toxins of Vibrios","volume":"3","author":"Satchell","year":"2015","journal-title":"Microbiol. Spectr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3220","DOI":"10.1128\/AEM.67.7.3220-3225.2001","article-title":"The mannose-sensitive hemagglutinin of Vibrio cholerae promotes adherence to zooplankton","volume":"67","author":"Chiavelli","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1099\/00221287-148-7-2181","article-title":"Cloning and characterization of a novel haemolysin in Vibrio cholerae O1 that does not directly contribute to the virulence of the organism","volume":"148","author":"Fallarino","year":"2002","journal-title":"Microbiology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"6555","DOI":"10.1128\/JB.00949-09","article-title":"The Vibrio cholerae flagellar regulatory hierarchy controls expression of virulence factors","volume":"191","author":"Syed","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1111\/j.1365-2958.1988.tb00054.x","article-title":"Extracellular proteins of Vibrio cholerae: Nucleotide sequence of the structural gene (hlyA) for the haemolysin of the haemolytic El Tor strain 017 and characterization of the hlyA mutation in the non-haemolytic classical strain 569B","volume":"2","author":"Alm","year":"1988","journal-title":"Mol. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1111\/j.1365-2958.2006.05544.x","article-title":"Antimicrobial peptides activate the Vibrio cholerae sigmaE regulon through an OmpU-dependent signalling pathway","volume":"63","author":"Mathur","year":"2007","journal-title":"Mol. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.tim.2016.12.003","article-title":"Rules of engagement: The type VI secretion system in Vibrio cholerae","volume":"25","author":"Joshi","year":"2017","journal-title":"Trends Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1038\/nature06284","article-title":"The major Vibrio cholerae autoinducer and its role in virulence factor production","volume":"450","author":"Higgins","year":"2007","journal-title":"Nature"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"4108","DOI":"10.1111\/j.1365-294X.2010.04809.x","article-title":"Novel insights into haemagglutinin protease (HAP) gene regulation in Vibrio cholerae","volume":"19","author":"Halpern","year":"2010","journal-title":"Mol. Ecol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1046\/j.1462-5822.2000.00025.x","article-title":"Distinct effects of Vibrio cholerae haemagglutinin\/protease on the structure and localization of the tight junction-associated proteins occludin and ZO-1","volume":"2","author":"Wu","year":"2000","journal-title":"Cell. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1128\/IAI.00982-12","article-title":"Novel cholix toxin variants, ADP-ribosylating toxins in Vibrio cholerae non-O1\/non-O139 strains, and their pathogenicity","volume":"81","author":"Awasthi","year":"2013","journal-title":"Infect. Immun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3577","DOI":"10.1128\/IAI.72.6.3577-3583.2004","article-title":"The Vibrio cholerae ToxR-regulated porin OmpU confers resistance to antimicrobial peptides","volume":"72","author":"Mathur","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3325","DOI":"10.1128\/iai.58.10.3325-3329.1990","article-title":"Cloning and nucleotide sequence of a heat-stable enterotoxin gene from Vibrio cholerae non-O1 isolated from a patient with traveler\u2019s diarrhea","volume":"58","author":"Ogawa","year":"1990","journal-title":"Infect. Immun."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1128\/AEM.67.2.910-921.2001","article-title":"Molecular analysis of Vibrio cholerae O1, O139, non-O1, and non-O139 strains: Clonal relationships between clinical and environmental isolates","volume":"67","author":"Singh","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1128\/AEM.67.6.2421-2429.2001","article-title":"Genotypes associated with virulence in environmental isolates of Vibrio cholerae","volume":"67","author":"Rivera","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"9798","DOI":"10.1073\/pnas.0401104101","article-title":"Identification of a domain within the multifunctional Vibrio cholerae RTX toxin that covalently cross-links actin","volume":"101","author":"Sheahan","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.1128\/aem.53.9.2262-2264.1987","article-title":"New selective and differential medium for Vibrio cholerae and Vibrio vulnificus","volume":"53","author":"Massad","year":"1987","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_58","unstructured":"Swayne, D.E. (2016). Miscellaneous and sporadic bacterial infections. Diseases of Poultry, Wiley-Blackwell. [13th ed.]."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e00831","DOI":"10.1128\/JCM.00831-18","article-title":"Evaluation of whole-genome sequencing for identification and typing of Vibrio cholerae","volume":"56","author":"Greig","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"W728","DOI":"10.1093\/nar\/gki415","article-title":"The multilocus sequence typing network: Mlst.net","volume":"33","author":"Aanensen","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1128\/AEM.03540-14","article-title":"Non-O1\/non-O139 Vibrio cholerae carrying multiple virulence factors and V. cholerae O1 in the Chesapeake Bay, Maryland","volume":"81","author":"Ceccarelli","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Baddam, R., Sarker, N., Ahmed, D., Mazumder, R., Abdullah, A., Morshed, R., Hussain, A., Begum, S., Shahrin, L., and Khan, A.I. (2020). Genome dynamics of Vibrio cholerae isolates linked to seasonal outbreaks of cholera in Dhaka, Bangladesh. mBio, 11.","DOI":"10.1128\/mBio.03339-19"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Bwire, G., Sack, D.A., Almeida, M., Li, S., Voeglein, J.B., Debes, A.K., Kagirita, A., Buyinza, A.W., Orach, C.G., and Stine, O.C. (2018). Molecular characterization of Vibrio cholerae responsible for cholera epidemics in Uganda by PCR, MLVA and WGS. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006492"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Siriphap, A., Leekitcharoenphon, P., Kaas, R.S., Theethakaew, C., Aarestrup, F.M., Sutheinkul, O., and Hendriksen, R.S. (2017). Characterization and genetic variation of Vibrio cholerae isolated from clinical and environmental sources in Thailand. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0169324"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1910","DOI":"10.1126\/science.272.5270.1910","article-title":"Lysogenic conversion by a filamentous phage encoding cholera toxin","volume":"272","author":"Waldor","year":"1996","journal-title":"Science"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"5097","DOI":"10.1128\/JB.182.18.5097-5104.2000","article-title":"Characterization of Vibrio cholerae O1 antigen as the bacteriophage K139 receptor and identification of IS1004 insertions aborting O1 antigen biosynthesis","volume":"182","author":"Nesper","year":"2000","journal-title":"J Bacteriol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1089\/dna.2008.0737","article-title":"Distribution of genes for virulence and ecological fitness among diverse Vibrio cholerae population in a cholera endemic area: Tracking the evolution of pathogenic strains","volume":"27","author":"Rahman","year":"2008","journal-title":"DNA Cell Biol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"e00795-17","DOI":"10.1128\/JB.00795-17","article-title":"Environmental role of pathogenic traits in Vibrio cholerae","volume":"200","author":"Sakib","year":"2018","journal-title":"J. Bacteriol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1107\/S2059798317017697","article-title":"Crystal structure of the outer membrane protein OmpU from Vibrio cholerae at 2.2 A resolution","volume":"74","author":"Li","year":"2018","journal-title":"Acta Cryst. D Struct. Biol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1099\/jmm.0.023051-0","article-title":"Antibiotic resistance mechanisms of Vibrio cholerae","volume":"60","author":"Kitaoka","year":"2011","journal-title":"J. Med. Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/j.ijmm.2014.07.010","article-title":"Pathogenic vibrios in environmental, seafood and clinical sources in Germany","volume":"304","author":"Huehn","year":"2014","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1007\/s00248-013-0221-4","article-title":"Temporal and spatial distribution patterns of potentially pathogenic Vibrio spp. at recreational beaches of the German north sea","volume":"65","author":"Boer","year":"2013","journal-title":"Microb. Ecol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"e00317","DOI":"10.1128\/AEM.00317-18","article-title":"Spatiotemporal dynamics of Vibrio cholerae in turbid alkaline lakes as determined by quantitative PCR","volume":"84","author":"Bliem","year":"2018","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/8\/1104\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:51:09Z","timestamp":1760176269000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/8\/1104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,23]]},"references-count":73,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,8]]}},"alternative-id":["microorganisms8081104"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms8081104","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,23]]}}}