{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:22:09Z","timestamp":1790716929538,"version":"4.1.0"},"reference-count":71,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T00:00:00Z","timestamp":1596067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"South Africa the Department of Agriculture, Land Reform and Rural Development","award":["21.1.1\/VPH-01\/OVI"],"award-info":[{"award-number":["21.1.1\/VPH-01\/OVI"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Meat products have been implicated in many listeriosis outbreaks globally, however there is a dearth of information on the diversity of L. monocytogenes isolates circulating in food products in South Africa. The aim of this study was to investigate the population structure of L. monocytogenes isolated in the meat value chain within the South African market. Based on whole-genome sequence analysis, a total of 217 isolates were classified into two main lineage groupings namely lineages I (n = 97; 44.7%) and II (n = 120; 55.3%). The lineage groups were further differentiated into IIa (n = 95, 43.8%), IVb (n = 69, 31.8%), IIb (n = 28, 12.9%), and IIc (n = 25, 11.5%) sero-groups. The most abundant sequence types (STs) were ST204 (n = 32, 14.7%), ST2 (n = 30, 13.8%), ST1 (n = 25, 11.5%), ST9 (n = 24, 11.1%), and ST321 (n = 21, 9.7%). In addition, 14 clonal complex (CCs) were identified with over-representation of CC1, CC3, and CC121 in \u201cProcessed Meat-Beef\u201d, \u201cRTE-Poultry\u201d, and \u201cRaw-Lamb\u201d meat categories, respectively. Listeria pathogenic islands were present in 7.4% (LIPI-1), 21.7% (LIPI-3), and 1.8% (LIPI-4) of the isolates. Mutation leading to premature stop codons was detected in inlA virulence genes across isolates identified as ST121 and ST321. The findings of this study demonstrated a high-level of genomic diversity among L. monocytogenes isolates recovered across the meat value chain control points in South Africa.<\/jats:p>","DOI":"10.3390\/microorganisms8081152","type":"journal-article","created":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T03:36:38Z","timestamp":1596080198000},"page":"1152","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Population Structure of Non-ST6 Listeria monocytogenes Isolated in the Red Meat and Poultry Value Chain in South Africa"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1495-357X","authenticated-orcid":false,"given":"Itumeleng","family":"Matle","sequence":"first","affiliation":[{"name":"Bacteriology Division, Agricultural Research Council-Onderstepoort Veterinary Research, Onderstepoort 0110, South Africa"},{"name":"Department of Agriculture and Animal Health, Science Campus, University of South Africa, Florida 1709, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4978-5692","authenticated-orcid":false,"given":"Thendo","family":"Mafuna","sequence":"additional","affiliation":[{"name":"Centre for Bioinformatics and Computational Biology, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria 0028, South Africa"},{"name":"Biotechnology Platform, Agricultural Research Council-Onderstepoort Veterinary Research, Private Bag X 05, Onderstepoort 0110, Pretoria, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5400-343X","authenticated-orcid":false,"given":"Evelyn","family":"Madoroba","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Zululand, KwaDlangezwa 3886, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9450-8646","authenticated-orcid":false,"given":"Khanyisile R.","family":"Mbatha","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Animal Health, Science Campus, University of South Africa, Florida 1709, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kudakwashe","family":"Magwedere","sequence":"additional","affiliation":[{"name":"Directorate of Veterinary Public Health, Department of Agriculture, Land Reform and Rural Development, Pretoria 0001, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0619-0058","authenticated-orcid":false,"given":"Rian","family":"Pierneef","sequence":"additional","affiliation":[{"name":"Biotechnology Platform, Agricultural Research Council-Onderstepoort Veterinary Research, Private Bag X 05, Onderstepoort 0110, Pretoria, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,30]]},"reference":[{"key":"ref_1","unstructured":"DAFF (2020, April 03). Abstract of Agricultural Statistics. Department of Agriculture, Forestry and Fisheries, Available online: https:\/\/www.daff.gov.za\/Daffweb3\/Portals\/0\/Statistics%20and%20Economic%20Analysis\/Statistical%20Information\/Abstract%202017.pdf."},{"key":"ref_2","unstructured":"Nyamakwere, F. (2015). Microbiological Analyses of Beef Slaughtering Process and Meat Safety Knowledge of Handlers at Selected High and Low Throughput Abattoirs. [Ph.D. Thesis, University of Fort Hare]."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"239","DOI":"10.4102\/ojvr.v71i3.266","article-title":"Towards the establishment and standardization of a veterinary antimicrobial resistance surveillance and monitoring programme in South Africa","volume":"71","author":"Nel","year":"2004","journal-title":"Onderstepoort. J. Vet. Res."},{"key":"ref_4","first-page":"380","article-title":"Genomes of sequence type 121 Listeria monocytogenes strains harbor highly conserved plasmids and prophages","volume":"6","author":"Maller","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/S1473-3099(14)70870-9","article-title":"The global burden of listeriosis: A systematic review and meta-analysis","volume":"14","author":"Devleesschauwer","year":"2014","journal-title":"Lancet. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1089\/fpd.2016.2274","article-title":"Listeria monocytogenes in food-processing facilities, food contamination, and human listeriosis: The Brazilian scenario","volume":"14","author":"Camargo","year":"2017","journal-title":"Foodborne. Pathog. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1089\/fpd.2018.2586","article-title":"Outbreak of Listeria monocytogenes in South Africa, 2017\u20132018: Laboratory Activities and Experiences Associated with Whole-Genome Sequencing Analysis of Isolates","volume":"16","author":"Smith","year":"2019","journal-title":"Foodborne. Pathog. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1056\/NEJMoa1907462","article-title":"Outbreak of listeriosis in South Africa associated with processed meat","volume":"382","author":"Thomas","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_9","first-page":"50","article-title":"An outbreak of listeriosis in cattle and sheep ingesting potato offcuts","volume":"70","year":"1999","journal-title":"Tydskr. S. Afr. Vet. Ver."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2004.06.009","article-title":"Contamination of chicken carcasses in Gauteng, South Africa, by Salmonella, Listeria monocytogenes and Campylobacter","volume":"99","author":"Marais","year":"2005","journal-title":"Int. J. Food. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.foodcont.2007.07.004","article-title":"Microbiological survey of ready-to-eat foods and associated preparation surfaces in retail delicatessens, Johannesburg, South Africa","volume":"19","author":"Christison","year":"2008","journal-title":"Food. Control."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.4315\/0362-028X.JFP-16-540","article-title":"Exploratory study into the microbiological quality of spinach and cabbage purchased from street vendors and retailers in Johannesburg, South Africa","volume":"80","author":"Plessis","year":"2017","journal-title":"J. Food Prot."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/jfs.12629","article-title":"Occurrence, serotypes, and characteristics of Listeria monocytogenes in meat and meat products in South Africa between 2014 and 2016","volume":"39","author":"Matle","year":"2019","journal-title":"J. Food. Saf."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6258","DOI":"10.1128\/AEM.01532-16","article-title":"Core genome multilocus sequence typing for identification of globally distributed clonal groups and differentiation of outbreak strains of Listeria monocytogenes","volume":"82","author":"Chen","year":"2016","journal-title":"Appl. Env. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/S1473-3099(16)30521-7","article-title":"Clinical features and prognostic factors of listeriosis: The MONALISA national prospective cohort study","volume":"17","author":"Charlier","year":"2017","journal-title":"Lancet. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.fm.2018.12.005","article-title":"Molecular characterization of Listeria monocytogenes isolates from a small- scale meat processor in Montenegro, 2011\u20132014","volume":"79","author":"Zuber","year":"2019","journal-title":"J. Food. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/851712","article-title":"Listeriosis during Pregnancy: A Public Health Concern","volume":"2013","author":"Mateus","year":"2013","journal-title":"ISRN Obs. Gynecol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Chlebicz, A., and \u015ali\u017cewska, K. (2018). Campylobacteriosis, Salmonellosis, Yersiniosis, and Listeriosis as Zoonotic Foodborne Diseases: A Review. Int. J. Env. Res. Public Health, 15.","DOI":"10.3390\/ijerph15050863"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/ng.3501","article-title":"Uncovering Listeria monocytogenes hypervirulence by harnessing its biodiversity","volume":"48","author":"Maury","year":"2016","journal-title":"Nat. Genet."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"16185","DOI":"10.1038\/nmicrobiol.2016.185","article-title":"Whole genome-based population biology and epidemiological surveillance of Listeria monocytogenes","volume":"2","author":"Moura","year":"2016","journal-title":"Nat. Microbio."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2017.00603","article-title":"Analysis of the Listeria monocytogenes population structure among isolates from 1931 to 2015 in Australia","volume":"8","author":"Jennison","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/mBio.00396-18","article-title":"Listeria monocytogenes source distribution analysis indicates regional heterogeneity and ecological niche preference among serotype 4b clones","volume":"9","author":"Lee","year":"2018","journal-title":"mBio"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.3201\/eid2309.170336","article-title":"Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France","volume":"23","author":"Moura","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1093\/bioinformatics\/btw586","article-title":"stringMLST: A fast k-mer based tool for multilocus sequence typing","volume":"33","author":"Gupta","year":"2017","journal-title":"Bioinformatics"},{"key":"ref_26","unstructured":"Seemann, T. (2020, April 09). mlst Github. Available online: https:\/\/github.com\/tseemann\/mlst."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: Rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1128\/AEM.01453-06","article-title":"A Single-Nucleotide-Polymorphism-Based Multilocus Genotyping Assay for Subtyping Lineage I Isolates of Listeria monocytogenes","volume":"73","author":"Ducey","year":"2006","journal-title":"Appl. Env. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/molbev\/msu300","article-title":"IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies","volume":"32","author":"Nguyen","year":"2015","journal-title":"Mol. Biol. Evol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/2041-210X.12628","article-title":"Ggtree: An R Package for Visualization and Annotation of Phylogenetic Trees With Their Covariates and Other Associated Data","volume":"8","author":"Yu","year":"2017","journal-title":"Methods. Ecol. Evol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1093\/nar\/gkf436","article-title":"MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform","volume":"30","author":"Katoh","year":"2002","journal-title":"Nucleic. Acids. Res."},{"key":"ref_32","unstructured":"R Core Team R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_33","first-page":"631","article-title":"The vegan package","volume":"10","author":"Oksanen","year":"2007","journal-title":"Community Ecol. Package"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1093\/bioinformatics\/bty633","article-title":"Ape 5.0: An environment for modern phylogenetics and evolutionary analyses in R","volume":"35","author":"Paradis","year":"2019","journal-title":"Bioinformatics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2869","DOI":"10.1128\/JCM.01193-15","article-title":"Defining and evaluating a core genome multilocus sequence typing scheme for whole-genome sequence-based typing of Listeria monocytogenes","volume":"53","author":"Ruppitsch","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_36","first-page":"1151E","article-title":"Closing gaps for performing a risk assessment on Listeria monocytogenes in ready-to-eat (RTE) foods: Activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis","volume":"14","author":"Nielsen","year":"2017","journal-title":"EFSA Support. Publ."},{"key":"ref_37","first-page":"1","article-title":"Retrospective validation of whole genome sequencingenhanced surveillance of listeriosis in Europe, 2010 to 2015","volume":"23","author":"Cormican","year":"2018","journal-title":"Eurosurveillance"},{"key":"ref_38","first-page":"378","article-title":"The effect of short-time microwave exposures on Salmonella typhimurium inoculated onto chicken drumettes","volume":"10","author":"Zeinali","year":"2009","journal-title":"Iran. J. Vet. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1128\/JCM.00606-13","article-title":"Optimized multilocus variable-number tandem-repeat analysis assay and its complementarity with pulsed-field gel electrophoresis and multilocus sequence typing for Listeria monocytogenes clone identification and surveillance","volume":"51","author":"Diancourt","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ijmm.2010.05.002","article-title":"Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics","volume":"301","author":"Orsi","year":"2011","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1292\/jvms.15-0470","article-title":"Multiplex PCR serogrouping of Listeria monocytogenes isolated in Japan","volume":"78","author":"Shimojima","year":"2016","journal-title":"J. Vet. Med. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1111\/j.1472-765X.2010.02928.x","article-title":"The characterization of Listeria spp. isolated from food products and the food-processing environment","volume":"51","author":"Leonard","year":"2010","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.bjm.2017.01.010","article-title":"Prevalence and serotype distribution of Listeria monocytogenes isolated from foods in Montevideo-Uruguay","volume":"48","author":"Braga","year":"2017","journal-title":"Braz. J. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.4315\/0362-028X.JFP-15-233","article-title":"Examination of Listeria monocytogenes in seafood processing facilities and smoked salmon in the Republic of Ireland","volume":"78","author":"Leong","year":"2015","journal-title":"J. Food. Prot."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1186\/s12866-015-0476-7","article-title":"Prevalence, antimicrobial susceptibility and virulotyping of Listeria species and Listeria monocytogenes isolated from open-air fish markets","volume":"15","author":"Jamali","year":"2015","journal-title":"BMC Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1111\/jam.12876","article-title":"Identification of high-risk Listeria monocytogenes serotypes in lineage I (serotype 1\/2a, 1\/2c, 3a and 3c) using multiplex PCR","volume":"119","author":"Nho","year":"2015","journal-title":"J. Appl. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1111\/1462-2920.12342","article-title":"The ubiquitous nature of Listeria monocytogenes clones: A large-scale Multilocus Sequence Typing study","volume":"16","author":"Haase","year":"2014","journal-title":"Env. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/1471-2180-12-119","article-title":"Genetic diversity and molecular typing of Listeria monocytogenes in China","volume":"12","author":"Wang","year":"2012","journal-title":"BMC Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2017.02351","article-title":"An assessment of different genomic approaches for inferring phylogeny of Listeria monocytogenes","volume":"8","author":"Henri","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1128\/JCM.02344-15","article-title":"Prospective whole-genome sequencing enhances national surveillance of Listeria monocytogenes","volume":"54","author":"Kwong","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.foodcont.2015.04.030","article-title":"Phenotypic and genotypic characteristics of Listeria monocytogenes strains isolated during 2011\u20132014 from different food matrices in Switzerland","volume":"57","author":"Ebner","year":"2015","journal-title":"Food Control"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1089\/fpd.2013.1578","article-title":"Collaborative survey on the colonization of different types of cheese-processing facilities with Listeria monocytogenes","volume":"11","author":"Stessl","year":"2014","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.fm.2014.05.014","article-title":"Diversity and distribution of Listeria monocytogenes in meat processing plants","volume":"44","author":"Perich","year":"2014","journal-title":"Food Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.ijfoodmicro.2007.12.012","article-title":"A survey of food-borne pathogens in free-range poultry farms","volume":"123","author":"Esteban","year":"2008","journal-title":"Int. J. Food. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2334-13-152","article-title":"Enhanced surveillance of invasive listeriosis in the Lombardy region, Italy, in the years 2006\u20132010 reveals major clones and an increase in serotype 1\/2a","volume":"13","author":"Mammina","year":"2013","journal-title":"BMC Infect. Dis."},{"key":"ref_56","first-page":"1","article-title":"Hypervirulent Listeria monocytogenes clones\u2019 adaption to mammalian gut accounts for their association with dairy products","volume":"10","author":"Maury","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_57","unstructured":"National Institute for Communicable Diseases (NICD) (2018, January 25). Listeria monocytogenes Outbreak 2017\/2018. Available online: https:\/\/www.bing.com\/search?q=+listeria+monocytogenes+outbreak+2017%2F2018.+situational+report+-+25th+january+2018+.&qs=n&form=QBRE&sp=-1&pq=listeria+monocytogenes+outbreak+2017%2F2018.+situational+report+-+25th+january+2018+.&sc=0-83&sk=&cvid=1CE774E330C44606A150173989DF3CAA."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12864-019-6399-1","article-title":"Genomic dissection of the most prevalent Listeria monocytogenes clone, sequence type ST87, in China","volume":"20","author":"Wang","year":"2019","journal-title":"BMC Genom."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.micres.2015.04.002","article-title":"Geographical and longitudinal analysis of Listeria monocytogenes genetic diversity reveals its correlation with virulence and unique evolution","volume":"175","author":"Yin","year":"2015","journal-title":"Microbiol. Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"4458","DOI":"10.1128\/AEM.70.8.4458-4467.2004","article-title":"Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment","volume":"70","author":"Nightingale","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e67511","DOI":"10.1371\/journal.pone.0067511","article-title":"Evolutionary Dynamics of the Accessory Genome of Listeria monocytogenes","volume":"8","author":"Desjardins","year":"2013","journal-title":"PLoS ONE"},{"key":"ref_62","first-page":"1","article-title":"Genetic diversity and virulence profiles of Listeria monocytogenes recovered from bulk tank milk, milk filters, and milking equipment from dairies in the United States (2002 to 2014)","volume":"13","author":"Kim","year":"2018","journal-title":"PLoS ONE"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12866-019-1488-5","article-title":"Genetic characteristics and virulence of Listeria monocytogenes isolated from fresh vegetables in China","volume":"19","author":"Chen","year":"2019","journal-title":"BMC Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.3201\/eid\/1706.101778","article-title":"Worldwide distribution of major clones of Listeria monocytogenes","volume":"17","author":"Lopez","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12974-018-1293-3","article-title":"Characterization of a Listeria monocytogenes meningitis mouse model","volume":"15","author":"Koopmans","year":"2018","journal-title":"J. Neuroinflammation"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"e26219","DOI":"10.4161\/mge.26219","article-title":"Toxin\u2013antitoxin systems","volume":"3","author":"Unterholzner","year":"2013","journal-title":"Mob. Genet. Elem."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Hilliard, A., Leong, D., O\u2019Callaghan, A., Culligan, E.P., Morgan, C.A., Delappe, N., Hill, C., Jordan, K., Cormican, M., and Gahan, C.G.M. (2018). Genomic characterization of Listeria monocytogenes isolates associated with clinical listeriosis and the food production environment in Ireland. Genes, 9.","DOI":"10.3390\/genes9030171"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.meegid.2008.04.006","article-title":"Lineage specific recombination and positive selection in coding and intragenic regions contributed to evolution of the main Listeria monocytogenes virulence gene cluster","volume":"8","author":"Orsi","year":"2008","journal-title":"Infect. Genet. Evoi."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1007\/s11684-017-0593-9","article-title":"Prevalence and molecular characteristics of Listeria monocytogenes in cooked products and its comparison with isolates from listeriosis cases","volume":"12","author":"Wang","year":"2018","journal-title":"Front. Med."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1128\/CMR.14.3.584-640.2001","article-title":"Listeria pathogenesis and molecular virulence determinants","volume":"14","author":"Kuhn","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"8764","DOI":"10.1128\/AEM.71.12.8764-8772.2005","article-title":"Select Listeria monocytogenes subtypes commonly found in foods carry distinct nonsense mutations in inlA","volume":"71","author":"Nightingale","year":"2005","journal-title":"Appl. Env. Microbiol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/8\/1152\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:52:48Z","timestamp":1760176368000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/8\/1152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,30]]},"references-count":71,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,8]]}},"alternative-id":["microorganisms8081152"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms8081152","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,30]]}}}