{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:52:28Z","timestamp":1790949148173,"version":"4.1.0"},"reference-count":51,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T00:00:00Z","timestamp":1597449600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Clofibric acid (CLF) is the main pharmacologically active metabolite in composition of the pharmaceutical products used for controlling blood lipid content. This xenobiotic compound is highly persistent in the aquatic environment and passes unchanged or poorly transformed in wastewater treatment plants. A white-rot fungal strain of Trametes pubescens was previously selected, for its ability for clofibric acid biodegradation (up to 30%) during cultivation in submerged system under aerobic conditions at an initial CLF concentration of 15 mg L\u22121. Plackett-Burman design (PBD) and response surface methodology (RSM) were used for experimental planning, mathematical modelling and statistical analysis of data of the biotechnological process of CLF biotransformation by Trametes pubescens fungal strain. After optimization, the capacity of the selected Trametes pubescens strain to degrade CLF was increased by cultivation in a liquid medium containing 3 g\u00b7L\u22121 yeast extract, 15 g\u00b7L\u22121 peptone, 5 g\u00b7L\u22121 glucose and mineral salts, inoculated at 2% (v\/v) vegetative inoculum and cultivated at pH 5.5, during 14 days at 25 \u00b0C and 135 rpm. In these optimized biotechnological conditions, the CLF biotransformation yield was 60%.<\/jats:p>","DOI":"10.3390\/microorganisms8081243","type":"journal-article","created":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T04:35:51Z","timestamp":1597638951000},"page":"1243","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Improving Biodegradation of Clofibric Acid by Trametes pubescens through the Design of Experimental Tools"],"prefix":"10.3390","volume":"8","author":[{"given":"Claudia Veronica","family":"Ungureanu","sequence":"first","affiliation":[{"name":"Cross-Border Faculty, Dun\u0103rea de Jos University, 800008 Galati, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7864-0062","authenticated-orcid":false,"given":"Lidia","family":"Favier","sequence":"additional","affiliation":[{"name":"Ecole Nationale Sup\u00e9rieure de Chimie de Rennes, CNRS, UMR 6226, Universit\u00e9 Europ\u00e9enne de Bretagne, CEDEX 7, 35708 Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8210-1793","authenticated-orcid":false,"given":"Gabriela Elena","family":"Bahrim","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Dun\u0103rea de Jos University, 800008 Galati, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1146\/annurev-environ-052809-161223","article-title":"Pharmaceuticals in the environment","volume":"35","author":"Kummerer","year":"2010","journal-title":"Annu. 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