{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:13:12Z","timestamp":1760238792075,"version":"build-2065373602"},"reference-count":19,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T00:00:00Z","timestamp":1599177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["JP17H04650","JP19K07532"],"award-info":[{"award-number":["JP17H04650","JP19K07532"]}]},{"name":"OHYAMA HEALTH FOUNDATION Inc.","award":["None"],"award-info":[{"award-number":["None"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The microscopic examination of Giemsa-stained thin and\/or thick blood films (Giemsa microscopy) is the standard method of malaria diagnosis. However, the results of the diagnosis significantly depend on the skills of clinical technicians. Furthermore, sample preparation and analysis are laborious and time-consuming. Therefore, in this study, we investigated if a commercially available fluorescent cell counter, LUNA-FL, was useful for the detection of Plasmodium parasite and the estimation of parasitemia. Whole blood samples from uninfected persons, spiked with P. falciparum-infected erythrocytes, were analysed. Most of the leucocytes and platelets were removed from whole blood samples with SiO2-nanofiber filters set on spin columns. The filtered samples were stained with acridine orange, and automatic detection, as well as counting of erythrocytes and parasites, were performed using LUNA-FL. Whole blood, with various levels of parasites, was analysed by Giemsa microscopy or with LUNA-FL to estimate parasitemia, and a comparative analysis was performed. The coefficient determination value of the regression line was high (R2 = 0.98), indicating that accurate quantitative parasite detection could be performed using LUNA-FL. LUNA-FL has a low running cost; it is compact, fast, and easy to operate, and may therefore be useful for point-of-care testing in the endemic areas.<\/jats:p>","DOI":"10.3390\/microorganisms8091356","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T11:24:24Z","timestamp":1599218664000},"page":"1356","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Quantitative Detection of Plasmodium falciparum Using, LUNA-FL, A Fluorescent Cell Counter"],"prefix":"10.3390","volume":"8","author":[{"given":"Muneaki","family":"Hashimoto","sequence":"first","affiliation":[{"name":"Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, Kagawa 761-0301, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8590-2737","authenticated-orcid":false,"given":"Kazumichi","family":"Yokota","sequence":"additional","affiliation":[{"name":"Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, Kagawa 761-0301, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuaki","family":"Kajimoto","sequence":"additional","affiliation":[{"name":"Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, Kagawa 761-0301, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Musashi","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Konica Minolta, 1 Sakura-mashi, Hino, Tokyo 191-8511, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuro","family":"Tatsumi","sequence":"additional","affiliation":[{"name":"Konica Minolta, 1 Sakura-mashi, Hino, Tokyo 191-8511, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichi","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Nitto Denko Corporation, 18, Hirayama, Nakahara-cho, Toyohashi, Aichi 441-3194, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomonori","family":"Hyodo","sequence":"additional","affiliation":[{"name":"Nitto Denko Corporation, 18, Hirayama, Nakahara-cho, Toyohashi, Aichi 441-3194, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiichiro","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Nitto Denko Corporation, 18, Hirayama, Nakahara-cho, Toyohashi, Aichi 441-3194, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noboru","family":"Minakawa","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiro","family":"Mita","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine and Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5230-8950","authenticated-orcid":false,"given":"Hiroaki","family":"Oka","sequence":"additional","affiliation":[{"name":"Konica Minolta, 1 Sakura-mashi, Hino, Tokyo 191-8511, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Kataoka","sequence":"additional","affiliation":[{"name":"Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, Kagawa 761-0301, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,4]]},"reference":[{"key":"ref_1","unstructured":"WHO (2010). Guidelines for the Treatment of Malaria, World Health Organization. [2nd ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1016\/S0140-6736(18)30324-6","article-title":"Malaria","volume":"391","author":"Ashley","year":"2018","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"119","DOI":"10.4269\/ajtmh.2007.77.119","article-title":"A review of malaria diagnostic tools: Microscopy and rapid diagnostic test (RDT)","volume":"77","author":"Wongsrichanalai","year":"2007","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ndour, P.A., Larr\u00e9ch\u00e9, S., Mouri, O., Argy, N., Gay, F., Roussel, C., Jaur\u00e9guiberry, S., Perillaud, C., Langui, D., and Biligui, S. (2017). Measuring the Plasmodium falciparum HRP2 protein in blood from artesunate-treated malaria patients predicts post-artesunate delayed hemolysis. Sci. Transl. Med., 9.","DOI":"10.1126\/scitranslmed.aaf9377"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1186\/1475-2875-10-194","article-title":"Assessment of LED fluorescence microscopy for the diagnosis of Plasmodium falciparum infections in Gabon","volume":"10","author":"Lenz","year":"2011","journal-title":"Malar J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0035-9203(97)90074-2","article-title":"Comparative evaluation of four techniques for the diagnosis of Plasmodium falciparum infections","volume":"91","author":"Craig","year":"1997","journal-title":"Trans. R. Soc Trop. Med. Hyg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/S0035-9203(96)90300-4","article-title":"Direct acridine orange fluorescence examination of blood slides compared to current techniques for malaria diagnosis","volume":"90","author":"Gay","year":"1996","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0169-4758(92)90093-H","article-title":"Rapid diagnosis of malaria by fluorescence microscopy","volume":"8","author":"Kawamoto","year":"1992","journal-title":"Parasitol. Today"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s12936-018-2214-8","article-title":"Improvement of malaria diagnostic system based on acridine orange staining","volume":"17","author":"Kimura","year":"2018","journal-title":"Malar J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.trsl.2017.12.004","article-title":"Image analysis and machine learning for detecting malaria","volume":"194","author":"Poostchi","year":"2018","journal-title":"Transl. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"30136","DOI":"10.1038\/srep30136","article-title":"Application of a cell microarray chip system for accurate, highly sensitive, and rapid diagnosis for malaria in Uganda","volume":"6","author":"Yatsushiro","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Yatsushiro, S., Yamamura, S., Yamaguchi, Y., Shinohara, Y., Tamiya, E., Horii, T., Baba, Y., and Kataoka, M. (2010). Rapid and highly sensitive detection of malaria-infected erythrocytes using a cell microarray chip. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0013179"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1186\/s12936-017-1975-9","article-title":"Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting","volume":"16","author":"Hashimoto","year":"2017","journal-title":"Malar J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1128\/JCM.02155-16","article-title":"Evaluation of the Parasight Platform for Malaria Diagnosis","volume":"55","author":"Eshel","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s12936-018-2208-6","article-title":"An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro","volume":"17","author":"Tougan","year":"2018","journal-title":"Malar J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.bios.2019.02.064","article-title":"Development of a highly sensitive, quantitative, and rapid detection system for Plasmodium falciparum-infected red blood cells using a fluorescent blue-ray optical system","volume":"132","author":"Yamamoto","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101941","DOI":"10.1016\/j.parint.2019.101941","article-title":"Nucleic acid purification from dried blood spot on FTA Elute Card provides template for polymerase chain reaction for highly sensitive Plasmodium detection","volume":"73","author":"Hashimoto","year":"2019","journal-title":"Parasitol. Int."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/s12936-016-1159-z","article-title":"Plasmodium falciparum histidine rich protein-2 diversity and the implications for PfHRP 2: Based malaria rapid diagnostic tests in Ghana","volume":"15","author":"Amoah","year":"2016","journal-title":"Malar J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/S1473-3099(06)70579-5","article-title":"The reliability of diagnostic techniques in the diagnosis and management of malaria in the absence of a gold standard","volume":"6","author":"Ochola","year":"2006","journal-title":"Lancet Infect. Dis."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/9\/1356\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:06:53Z","timestamp":1760177213000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/9\/1356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,4]]},"references-count":19,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["microorganisms8091356"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms8091356","relation":{},"ISSN":["2076-2607"],"issn-type":[{"type":"electronic","value":"2076-2607"}],"subject":[],"published":{"date-parts":[[2020,9,4]]}}}