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Jovenes Profesores e Investigadores"],"award-info":[{"award-number":["Becas Iberoam\u00e9rica Movilizaci\u00f3n. Jovenes Profesores e Investigadores"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The aim of this study was to characterize carbapenem-resistant Klebsiella pneumoniae (CR-Kp) isolates recovered from adults and children with severe bacteremia in a Peruvian Hospital in June 2018. Antimicrobial susceptibility was determined by disc\/gradient diffusion and broth microdilution when necessary. Antibiotic resistance mechanisms were evaluated by PCR and DNA sequencing. Clonal relatedness was assessed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Plasmid typing was performed with a PCR-based method. Thirty CR-Kp isolates were recovered in June 2018. All isolates were non-susceptible to all \u03b2-lactams, ciprofloxacin, gentamicin and trimethoprim-sulfamethoxazole, while mostly remaining susceptible to colistin, tigecycline, levofloxacin and amikacin. All isolates carried the blaNDM-1 gene and were extended spectrum \u03b2-lactamase (ESBL) producers. PFGE showed four different pulsotypes although all isolates but two belonged to the ST348 sequence type, previously reported in Portugal. blaNDM-1 was located in an IncFIB-M conjugative plasmid. To our knowledge, this is the first report of an New Delhi metallo-\u03b2-lactamase (NDM)-producing K. pneumoniae recovered from both children and adults in Lima, Peru, as well as the first time that the outbreak strain ST348 is reported in Peru and is associated with NDM. Studies providing epidemiological and molecular data on CR-Kp in Peru are essential to monitor their dissemination and prevent further spread.<\/jats:p>","DOI":"10.3390\/microorganisms8091392","type":"journal-article","created":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T09:05:16Z","timestamp":1599815116000},"page":"1392","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Spread of ST348 Klebsiella pneumoniae Producing NDM-1 in a Peruvian Hospital"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8384-2315","authenticated-orcid":false,"given":"Maria J.","family":"Pons","sequence":"first","affiliation":[{"name":"Facultad de Medicina, Universidad Cient\u00edfica del Sur, Carr. Antigua Panamericana Sur 19, Villa El Salvador, Lima 15067, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7265-735X","authenticated-orcid":false,"given":"Marta","family":"Mar\u00ed-Almirall","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, ISGlobal, Hospital Cl\u00ednic, Universitat de Barcelona, Rossell\u00f3 149-153, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6482-9243","authenticated-orcid":false,"given":"Barbara","family":"Yma\u00f1a","sequence":"additional","affiliation":[{"name":"Facultad de Medicina, Universidad Cient\u00edfica del Sur, Carr. Antigua Panamericana Sur 19, Villa El Salvador, Lima 15067, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeel","family":"Moya-Salazar","sequence":"additional","affiliation":[{"name":"Hospital Nacional Docente Madre Ni\u00f1o San Bartolom\u00e9, Avenida Alfonso Ugarte 825, Lima 150101, Peru"},{"name":"Escuela de Posgrado, Facultad de Ciencias de la Salud, Universidad Privada San Juan Bautista, Ex Hacienda Villa, Av Jos\u00e9 Antonio Lavalle s\/n, Lima 15067, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, ISGlobal, Hospital Cl\u00ednic, Universitat de Barcelona, Rossell\u00f3 149-153, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Sau\u00f1e","sequence":"additional","affiliation":[{"name":"Escuela de Posgrado, Facultad de Ciencias de la Salud, Universidad Privada San Juan Bautista, Ex Hacienda Villa, Av Jos\u00e9 Antonio Lavalle s\/n, Lima 15067, Peru"},{"name":"Servicio de Citolog\u00eda y Citogen\u00e9tica, Departamento de Anatom\u00eda Patol\u00f3gica, Hospital Nacional Guillermo Almenara Irigoyen, Jir\u00f3n Garc\u00eda Naranjo 840, La Victoria 13, Lima 150115, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Salazar-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Servicio de Citolog\u00eda y Citogen\u00e9tica, Departamento de Anatom\u00eda Patol\u00f3gica, Hospital Nacional Guillermo Almenara Irigoyen, Jir\u00f3n Garc\u00eda Naranjo 840, La Victoria 13, Lima 150115, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Vila","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, ISGlobal, Hospital Cl\u00ednic, Universitat de Barcelona, Rossell\u00f3 149-153, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignasi","family":"Roca","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, ISGlobal, Hospital Cl\u00ednic, Universitat de Barcelona, Rossell\u00f3 149-153, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.mib.2018.04.004","article-title":"Klebsiella pneumoniae as a key trafficker of drug resistance genes from environmental to clinically important bacteria","volume":"45","author":"Wyres","year":"2018","journal-title":"Curr. 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