{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T22:29:11Z","timestamp":1787869751500,"version":"build-2784847793"},"reference-count":87,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T00:00:00Z","timestamp":1600992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018M3A9F3021964"],"award-info":[{"award-number":["2018M3A9F3021964"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016M3A9A5923160"],"award-info":[{"award-number":["2016M3A9A5923160"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The bacteriocin-producing strain Enterococcus faecium ST10Bz, isolated from boza, a Bulgarian cereal-based beverage, exhibited strong activity against Listeria strains, vancomycin-resistant and other Enterococcus strains, but not against most of the other lactic acid bacteria (LAB) strains included in the test panel. Bacteriocin ST10Bz was proven as a stable antimicrobial, even after exposure to various environmental conditions, including varying pH values, temperatures, and commonly used chemicals in industry and laboratory practice. Bacteriocin activity against L. monocytogenes ATCC\u00ae15313\u2122 was recorded at 25,600 AU\/mL when the producer strain was cultured in MRS broth at 25 \u00b0C and 30 \u00b0C, and 19,200 AU\/mL, when cultured at 37 \u00b0C. Additionally, bacteriocin ST10Bz exhibited bactericidal mode of action when added to actively growing cultures of L. monocytogenes ATCC\u00ae15313\u2122 and Enterococcus faecalis 200A. E. faecium ST10Bz was susceptible to the antibiotics kanamycin, gentamycin, ampicillin, streptomycin, tylosin, chloramphenicol, clindamycin, tetracycline, and vancomycin; with no evidence for vanA, B, C, D, E, or G genes. PCR analysis of DNA from strain ST10Bz generated positive results for presence of some bacterial adhesion genes, including map, mub and ef-tu, as well as the gamma aminobutyric acid (GABA) production-related gene, gad. Under simulated gastrointestinal conditions in single and co-culture with L. monocytogenes ATCC\u00ae15313\u2122 and E. faecalis 200A, E. faecium ST10Bz showed a high survival rate and the ability to reduce the viable numbers of the two test strains.<\/jats:p>","DOI":"10.3390\/microorganisms8101474","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T08:57:32Z","timestamp":1601024252000},"page":"1474","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Exploring Beneficial Properties of the Bacteriocinogenic Enterococcus faecium ST10Bz Strain Isolated from Boza, a Bulgarian Cereal-Based Beverage"],"prefix":"10.3390","volume":"8","author":[{"given":"Samantha Joy D.","family":"Valledor","sequence":"first","affiliation":[{"name":"ProBacLab, Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang, Gyeongbuk 791-708, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2897-7385","authenticated-orcid":false,"given":"Jorge Enrique Vazquez","family":"Bucheli","sequence":"additional","affiliation":[{"name":"ProBacLab, Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang, Gyeongbuk 791-708, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3669-1419","authenticated-orcid":false,"given":"Wilhelm H.","family":"Holzapfel","sequence":"additional","affiliation":[{"name":"ProBacLab, Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang, Gyeongbuk 791-708, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6377-3305","authenticated-orcid":false,"given":"Svetoslav Dimitrov","family":"Todorov","sequence":"additional","affiliation":[{"name":"ProBacLab, Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang, Gyeongbuk 791-708, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lahtinen, S., Ouwehand, A., Salminen, S., and Wright, A.V. 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