{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T10:06:23Z","timestamp":1780049183173,"version":"3.53.1"},"reference-count":21,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T00:00:00Z","timestamp":1601942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. Herein, we evaluated the relationship between the gut microbiome (GM) and disease phenotype by an integrated omics fused approach. In a multicenter, observational cohort study, stools from Italian JIA patients were collected at baseline, active, and inactive disease stages, and their GM compared to healthy controls (CTRLs). The microbiota metabolome was analyzed to detect volatile- and non-volatile organic compounds (VOCs); the data were fused with operational taxonomic units (OTUs) from 16S RNA targeted-metagenomics and classified by chemometric models. Non-VOCs did not characterize JIA patients nor JIA activity stages compared to CTRLs. The core of VOCs, (Ethanol, Methyl-isobutyl-ketone, 2,6-Dimethyl-4-heptanone and Phenol) characterized patients at baseline and inactive disease stages, while the OTUs represented by Ruminococcaceae, Lachnospiraceae and Clostridiacea discriminated between JIA inactive stage and CTRLs. No differences were highlighted amongst JIA activity stages. Finally, the fused data discriminated inactive and baseline stages versus CTRLs, based on the contribution of the invariant core of VOCs while Ruminococcaceae concurred for the inactive stage versus CTRLs comparison. In conclusion, the GM signatures enabled to distinguish the inactive disease stage from CTRLs.<\/jats:p>","DOI":"10.3390\/microorganisms8101540","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T09:12:58Z","timestamp":1602061978000},"page":"1540","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Fused Omics Data Models Reveal Gut Microbiome Signatures Specific of Inactive Stage of Juvenile Idiopathic Arthritis in Pediatric Patients"],"prefix":"10.3390","volume":"8","author":[{"given":"Pamela","family":"Vernocchi","sequence":"first","affiliation":[{"name":"Area of Genetics and Rare Diseases, Unit of Human Microbiome, Bambino Ges\u00f9 Children\u2019s Hospital, IRCCS, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8266-1117","authenticated-orcid":false,"given":"Federico","family":"Marini","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Sapienza University of Rome, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6992-2578","authenticated-orcid":false,"given":"Giorgio","family":"Capuani","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Sapienza University of Rome, 00185 Rome, Italy"},{"name":"NMR-Based Metabolomics Laboratory, Sapienza University of Rome, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberta","family":"Tomassini","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Sapienza University of Rome, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Conta","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Sapienza University of Rome, 00185 Rome, Italy"},{"name":"NMR-Based Metabolomics Laboratory, Sapienza University of Rome, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4204-4736","authenticated-orcid":false,"given":"Federica","family":"Del Chierico","sequence":"additional","affiliation":[{"name":"Area of Genetics and Rare Diseases, Unit of Human Microbiome, Bambino Ges\u00f9 Children\u2019s Hospital, IRCCS, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Malattia","sequence":"additional","affiliation":[{"name":"Seconda Divisione di Pediatria, Istituto Giannina Gaslini and Universit\u00e0 degli Studi di Genoa, 16147 Genoa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"De Benedetti","sequence":"additional","affiliation":[{"name":"Department of Pediatric Subspecialties, Division of Nephrology, Bambino Ges\u00f9 Children\u2019s Hospital, IRCCS, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Martini","sequence":"additional","affiliation":[{"name":"Seconda Divisione di Pediatria, Istituto Giannina Gaslini and Universit\u00e0 degli Studi di Genoa, 16147 Genoa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Dallapiccola","sequence":"additional","affiliation":[{"name":"Scientific Directorate, Bambino Ges\u00f9 Children\u2019s Hospital, IRCCS, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. H. Pieter","family":"van Dijkhuizen","sequence":"additional","affiliation":[{"name":"Seconda Divisione di Pediatria, Istituto Giannina Gaslini and Universit\u00e0 degli Studi di Genoa, 16147 Genoa, Italy"},{"name":"Department of Pediatrics, University Medical Center Utrecht, Wilhelmina Children\u2019s Hospital, 3584 EA Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"Miccheli","sequence":"additional","affiliation":[{"name":"NMR-Based Metabolomics Laboratory, Sapienza University of Rome, 00185 Rome, Italy"},{"name":"Department of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0134-2830","authenticated-orcid":false,"given":"Lorenza","family":"Putignani","sequence":"additional","affiliation":[{"name":"Unit of Parasitology and Area of Genetics and Rare Diseases, Unit of Human Microbiome, Department of Laboratories, Bambino Ges\u00f9 Children\u2019s Hospital, IRCCS, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1007\/s11926-012-0314-y","article-title":"The role of the microbiome in rheumatic diseases","volume":"15","author":"Yeoh","year":"2013","journal-title":"Curr. 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