{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:19:44Z","timestamp":1760239184883,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Microbial adhesion is critical for parasitic infection and colonization of host cells. To study the host\u2013parasite interaction in vitro, we established a flow cytometry-based assay to measure the adherence of Trichomonas vaginalis to epithelial cell line SiHa. SiHa cells and T. vaginalis were detected as clearly separated, quantifiable populations by flow cytometry. We found that T. vaginalis attached to SiHa cells as early as 30 min after infection and the binding remained stable up to several hours, allowing for analysis of drug treatment efficacy. Importantly, NADPH oxidase inhibitor DPI treatment induced the detachment of T. vaginalis from SiHa cells in a dose-dependent manner without affecting host cell viability. Thus, this study may provide an understanding for the potential development of therapies against T. vaginalis and other parasite infections.<\/jats:p>","DOI":"10.3390\/microorganisms8101570","type":"journal-article","created":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T21:48:38Z","timestamp":1602625718000},"page":"1570","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adherence of Trichomonas vaginalis to SiHa Cells is Inhibited by Diphenyleneiodonium"],"prefix":"10.3390","volume":"8","author":[{"given":"Yeeun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Medical Science, College of Medicine, Chungnam National University, 266 Munhwa-ro, Jung-gu, Daejeon 35015, Korea"},{"name":"Department of Infection Biology, College of Medicine, Chungnam National University, Daejeon 35015, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Ha","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medical Science, College of Medicine, Chungnam National University, 266 Munhwa-ro, Jung-gu, Daejeon 35015, Korea"},{"name":"Department of Infection Biology, College of Medicine, Chungnam National University, Daejeon 35015, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In-Wook","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Medical Science, College of Medicine, Chungnam National University, 266 Munhwa-ro, Jung-gu, Daejeon 35015, Korea"},{"name":"Department of Infection Biology, College of Medicine, Chungnam National University, Daejeon 35015, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bu Yeon","family":"Heo","sequence":"additional","affiliation":[{"name":"Department of Medical Science, College of Medicine, Chungnam National University, 266 Munhwa-ro, Jung-gu, Daejeon 35015, Korea"},{"name":"Department of Infection Biology, College of Medicine, Chungnam National University, Daejeon 35015, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju-Gyeong","family":"Kang","sequence":"additional","affiliation":[{"name":"Cardiovascular Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA"},{"name":"Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0630-9194","authenticated-orcid":false,"given":"Jae-Min","family":"Yuk","sequence":"additional","affiliation":[{"name":"Department of Medical Science, College of Medicine, Chungnam National University, 266 Munhwa-ro, Jung-gu, Daejeon 35015, Korea"},{"name":"Department of Infection Biology, College of Medicine, Chungnam National University, Daejeon 35015, Korea"},{"name":"Infection Control Convergence Research Center, College of Medicine, Chungnam National University, Daejeon 35015, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang-Ho","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Medical Science, College of Medicine, Chungnam National University, 266 Munhwa-ro, Jung-gu, Daejeon 35015, Korea"},{"name":"Department of Infection Biology, College of Medicine, Chungnam National University, Daejeon 35015, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun-Kyeong","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Medical Science, College of Medicine, Chungnam National University, 266 Munhwa-ro, Jung-gu, Daejeon 35015, Korea"},{"name":"Infection Control Convergence Research Center, College of Medicine, Chungnam National University, Daejeon 35015, Korea"},{"name":"Department of Microbiology, Collage of Medicine, Chungnam National University, Daejeon 35015, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2452-4111","authenticated-orcid":false,"given":"Jaeyul","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Medical Science, College of Medicine, Chungnam National University, 266 Munhwa-ro, Jung-gu, Daejeon 35015, Korea"},{"name":"Department of Infection Biology, College of Medicine, Chungnam National University, Daejeon 35015, Korea"},{"name":"Department of Medical Education, College of Medicine, Chungnam National University, Daejeon 35015, Korea"},{"name":"Translational Immunology Institute, Chungnam National University, Daejeon 35015, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.pt.2018.05.006","article-title":"Trichomonas vaginalis: Pathogenesis, symbiont interactions, and host cell 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