{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T09:51:19Z","timestamp":1781430679533,"version":"3.54.1"},"reference-count":60,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T00:00:00Z","timestamp":1603756800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["JS"],"award-info":[{"award-number":["JS"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010784","name":"Ocean Frontier Institute","doi-asserted-by":"publisher","award":["J.3."],"award-info":[{"award-number":["J.3."]}],"id":[{"id":"10.13039\/501100010784","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Vibrio anguillarum is a Gram-negative marine pathogen causative agent of vibriosis in a wide range of hosts, including invertebrates and teleosts. Lumpfish (Cyclopterus lumpus), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to control sea lice (Lepeophtheirus salmonis) infestations in the Atlantic salmon (Salmo salar) aquaculture industry. V. anguillarum is one of the most frequent bacterial pathogens affecting lumpfish. Here, we described the phenotype and genomic characteristics of V. anguillarum strain J360 isolated from infected cultured lumpfish in Newfoundland, Canada. Koch\u2019s postulates determined in na\u00efve lumpfish showed lethal acute vibriosis in lumpfish. The V. anguillarum J360 genome was shown to be composed of two chromosomes and two plasmids with a total genome size of 4.56 Mb with 44.85% G + C content. Phylogenetic and comparative analyses showed that V. anguillarum J360 is closely related to V. anguillarum strain VIB43, isolated in Scotland, with a 99.8% genome identity. Differences in the genomic organization were identified and associated with insertion sequence elements (ISs). Additionally, V. anguillarum J360 does not possess a pJM1-like plasmid, typically present in virulent isolates from the Pacific Ocean, suggesting that acquisition of this extrachromosomal element and the virulence of V. anguillarum J360 or other Atlantic isolates could increase.<\/jats:p>","DOI":"10.3390\/microorganisms8111666","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T09:22:45Z","timestamp":1603790565000},"page":"1666","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish (Cyclopterus lumpus) in Newfoundland Reveal Novel Chromosomal Organizations"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1390-2809","authenticated-orcid":false,"given":"Ignacio","family":"Vasquez","sequence":"first","affiliation":[{"name":"Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University, Logy Bay, NL A1C 5S7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trung","family":"Cao","sequence":"additional","affiliation":[{"name":"Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University, Logy Bay, NL A1C 5S7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Setu","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University, Logy Bay, NL A1C 5S7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajarooba","family":"Gnanagobal","sequence":"additional","affiliation":[{"name":"Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University, Logy Bay, NL A1C 5S7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"O\u2019Brien","sequence":"additional","affiliation":[{"name":"Department of Fisheries and Land Resources, Aquatic Animal Health Division, Government of Newfoundland and Labrador, St. John\u2019s, NL A1E 3Y5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Monk","sequence":"additional","affiliation":[{"name":"Dr. Joe Brown Aquatic Research Building (JBARB), Department of Ocean Sciences, Memorial University of Newfoundland, Logy Bay, NL A1C 5S7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danny","family":"Boyce","sequence":"additional","affiliation":[{"name":"Dr. Joe Brown Aquatic Research Building (JBARB), Department of Ocean Sciences, Memorial University of Newfoundland, Logy Bay, NL A1C 5S7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jillian D.","family":"Westcott","sequence":"additional","affiliation":[{"name":"Fisheries and Marine Institute, Memorial University of Newfoundland, St. John\u2019s, NL A1C 5R3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7701-3147","authenticated-orcid":false,"given":"Javier","family":"Santander","sequence":"additional","affiliation":[{"name":"Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University, Logy Bay, NL A1C 5S7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,27]]},"reference":[{"key":"ref_1","unstructured":"Pruzzo, C., Huq, A., Colwell, R., and Donelli, G. 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