{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T08:35:25Z","timestamp":1781598925184,"version":"3.54.5"},"reference-count":28,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T00:00:00Z","timestamp":1604102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>By late March 2020, Villa Caldari, a small village of the municipality of Ortona (Abruzzo region), was registering an incidence rate of COVID-19 cases ten times greater than the overall municipality and was declared a hotspot area. Twenty-two days later, epidemiological investigation and sampling were performed, to evaluate SARS-CoV-2 circulation and the presence of SARS-CoV-2 antibodies. Overall, 681 nasopharyngeal swabs and 667 blood samples were collected. Only one resident of the village resulted in being positive for RNA viral shedding, while 73 were positive for SARS-CoV-2 antibodies. The overall seroprevalence was 10.9%. The difference between the seroprevalence of infection in asymptomatic and symptomatic individuals was significant (\u03c72 = 14.50 p-value = 0.0001). Amongst the residents positive for antibodies, fatigue and\/or muscle pain, fever and anosmia were the most experienced symptoms, whose most frequent onset was observed during the first two weeks of March. Familial and habit-related clusters were highlighted. Nevertheless, the investigations showed a low SARS-CoV-2 circulation in the village at the time of the sampling, demonstrating virus transmission could be limited when strict emergency measures are followed. Given the favorable results, the emergency measures were then lifted.<\/jats:p>","DOI":"10.3390\/microorganisms8111711","type":"journal-article","created":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T21:39:56Z","timestamp":1604180396000},"page":"1711","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A COVID-19 Hotspot Area: Activities and Epidemiological Findings"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4982-476X","authenticated-orcid":false,"given":"Francesca","family":"Cito","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise G. Caporale, 64100 Teramo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Amato","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise G. 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Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41586-020-2012-7","article-title":"A Pneumonia Outbreak Associated with a New Coronavirus of Probable Bat Origin","volume":"579","author":"Zhou","year":"2020","journal-title":"Nature"},{"key":"ref_4","unstructured":"World Health Organization (WHO) (2020, May 13). WHO Director-General\u2019s remarks at the media briefing on 2019-nCoV-11 February 2020. Available online: https:\/\/www.who.int\/dg\/speeches\/detail\/who-director-general-s-remarks-at-the-media-briefing-on-2019-ncov-on-11-february-2020."},{"key":"ref_5","unstructured":"World Health Organization (WHO) (2020, May 15). WHO Director-General\u2019s opening remarks at the media briefing on COVID-19-11 March 2020. Available online: https:\/\/www.who.int\/dg\/speeches\/detail\/who-director-general-s-opening-remarks-at-the-media-briefing-on-covid-19-11-march-2020."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/s41591-020-0897-1","article-title":"Antibody Responses to SARS-CoV-2 in Patients with COVID-19","volume":"26","author":"Long","year":"2020","journal-title":"Nat. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"100135","DOI":"10.1016\/j.onehlt.2020.100135","article-title":"A \u201cOne-Health\u201d Approach for Diagnosis and Molecular Characterization of SARS-CoV-2 in Italy","volume":"10","author":"Lorusso","year":"2020","journal-title":"One Heal."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"10484","DOI":"10.1073\/pnas.2004978117","article-title":"Spread and Dynamics of the COVID-19 Epidemic in Italy: Effects of Emergency Containment Measures","volume":"117","author":"Gatto","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1016\/j.jaccas.2020.03.012","article-title":"(IP) The Outbreak of COVID-19 in Italy","volume":"2","author":"Indolfi","year":"2020","journal-title":"JACC Case Rep."},{"key":"ref_10","unstructured":"Gazzetta Ufficiale della Repubblica Italiana (2020, May 22). Decreto del Presidente del Consiglio dei Ministri 9 marzo 2020. Available online: https:\/\/www.gazzettaufficiale.it\/eli\/id\/2020\/03\/09\/20A01558\/sg."},{"key":"ref_11","first-page":"23","article-title":"Coronavirus Disease (COVID-19) in Italy: Analysis of Risk Factors and Proposed Remedial Measures","volume":"7","year":"2020","journal-title":"Front. Med."},{"key":"ref_12","unstructured":"Regione Abruzzo (2020, May 13). Ordinanza Del Presidente Della Giunta Regionale-n. 17 Del 27 Marzo 2020. Available online: https:\/\/www.regione.abruzzo.it\/system\/files\/atti-presidenziali\/2020\/ordinanza-n17-2020-1.pdf."},{"key":"ref_13","unstructured":"Regione Abruzzo (2020, May 13). Ordinanza Del Presidente Della Giunta Regionale-n. 10 Del 18 Marzo 2020. Available online: https:\/\/www.regione.abruzzo.it\/system\/files\/atti-presidenziali\/2020\/ordinanza-10-2020-1.pdf."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Danzetta, M.L., Amato, L., Cito, F., Di Giuseppe, A., Morelli, D., Savini, G., Mercante, M.T., Lorusso, A., Portanti, O., and Puglia, I. (2020). SARS-CoV-2 RNA Persistence in Naso-Pharyngeal Swabs. Microorganisms, 8.","DOI":"10.3390\/microorganisms8081124"},{"key":"ref_15","first-page":"5","article-title":"Novel Coronavirus (COVID-19) Epidemic: A Veterinary Perspective","volume":"24","author":"Lorusso","year":"2020","journal-title":"Vet. Ital."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lassauni\u00e8re, R., Frische, A., Harboe, Z.B., Nielsen, A.C.Y., Fomsgaard, A., Krogfelt, K.A., and J\u00f8rgensen, C.S. (2020). Evaluation of Nine Commercial SARS-CoV-2 Immunoassays. medRxiv.","DOI":"10.1101\/2020.04.09.20056325"},{"key":"ref_17","unstructured":"ISTAT, and Ministero della Salute (2020, August 23). Primi Risultati Dell\u2019indagine Di Sieroprevalenza Sul SARS-CoV-2, Available online: https:\/\/www.istat.it\/it\/files\/2020\/08\/ReportPrimiRisultatiIndagineSiero.pdf."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/S0140-6736(20)31483-5","article-title":"Prevalence of SARS-CoV-2 in Spain (ENE-COVID): A Nationwide, Population-Based Seroepidemiological Study","volume":"396","author":"Oteo","year":"2020","journal-title":"Lancet"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/s41586-020-2488-1","article-title":"Suppression of a SARS-CoV-2 Outbreak in the Italian Municipality of Vo\u2019","volume":"584","author":"Lavezzo","year":"2020","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/S1473-3099(20)30273-5","article-title":"Connecting Clusters of COVID-19: An Epidemiological and Serological Investigation","volume":"20","author":"Yong","year":"2020","journal-title":"Lancet Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0140-6736(20)31304-0","article-title":"Seroprevalence of Anti-SARS-CoV-2 IgG Antibodies in Geneva, Switzerland (SEROCoV-POP): A Population-Based Study","volume":"396","author":"Stringhini","year":"2020","journal-title":"Lancet"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/S0140-6736(20)31482-3","article-title":"SARS-CoV-2 Seroprevalence in COVID-19 Hotspots","volume":"396","author":"Eckerle","year":"2020","journal-title":"Lancet"},{"key":"ref_23","unstructured":"CDC (2020, August 07). Symptoms of Coronavirus, Available online: https:\/\/www.cdc.gov\/coronavirus\/2019-ncov\/symptoms-testing\/symptoms.html."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ijid.2020.03.017","article-title":"Prevalence of Comorbidities and Its Effects in Coronavirus Disease 2019 Patients: A Systematic Review and Meta-Analysis","volume":"94","author":"Yang","year":"2020","journal-title":"Int. J. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zayet, S., Klopfenstein, T., Mercier, J., Kadiane-Oussou, N.J., Lan Cheong Wah, L., Royer, P.Y., Toko, L., and Gendrin, V. (2020). Contribution of Anosmia and Dysgeusia for Diagnostic of COVID-19 in Outpatients. Infection.","DOI":"10.1007\/s15010-020-01442-3"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1159\/000509143","article-title":"Predictive Value of Sudden Olfactory Loss in the Diagnosis of COVID-19","volume":"82","author":"Haehner","year":"2020","journal-title":"ORL"},{"key":"ref_27","unstructured":"Istituto Superiore di Sanit\u00e0 (2020, October 19). CS N \u00b0 39\/2020-ISS study on wastewater, in Milan and Turin Sars-Cov-2 already present in December-ISS (EN)-ISS. Available online: https:\/\/www.iss.it\/web\/iss-en\/newsroom\/-\/asset_publisher\/u0TWLJl3A9rH\/content\/id\/5436642."},{"key":"ref_28","unstructured":"Regione Abruzzo (2020, May 13). Ordinanza Del Presidente Della Giunta Regionale-n. 45 Del 22 Aprile 2020. Available online: https:\/\/www.regione.abruzzo.it\/system\/files\/atti-presidenziali\/2020\/ordinanza-n-45-2020.pdf."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/11\/1711\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:27:40Z","timestamp":1760178460000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/8\/11\/1711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,31]]},"references-count":28,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11]]}},"alternative-id":["microorganisms8111711"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms8111711","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,31]]}}}