{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T02:48:25Z","timestamp":1790995705316,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,11,14]],"date-time":"2020-11-14T00:00:00Z","timestamp":1605312000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chilean Antarctic Institute (INACH)","award":["project code RT_02_16."],"award-info":[{"award-number":["project code RT_02_16."]}]},{"name":"National Fund for Scientific and Technological Development (FONDECYT)","award":["project no. 1201386"],"award-info":[{"award-number":["project no. 1201386"]}]},{"name":"SATREPS-MACH JST\/JICA","award":["JPMJSA1705"],"award-info":[{"award-number":["JPMJSA1705"]}]},{"name":"Apoyo a Profesores Patrocinantes de Alumnos de Pre y Postgrado","award":["DI19-2016"],"award-info":[{"award-number":["DI19-2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>1-Aminociclopropane-1-carboxylate (ACC)-degrading bacteria having been widely studied for their use in alleviating abiotic stresses in plants. In the present study, we isolated and characterized ACC-degrading bacteria from the rhizosphere, phyllosphere, and endosphere of the Antarctic vascular plants Deschampsia antarctica and Colobanthus quitensis. One hundred and eighty of the 578 isolates (31%) were able to grow on minimal medium containing ACC, with 101 isolates (23, 37, and 41 endosphere-, phyllosphere- and rhizosphere-associated isolates, respectively) identified as being genetically unique by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Subsequently, freeze\/thaw treatments and ice-recrystallization-inhibition (IRI) activity assays were performed, the results of which revealed that 77 (13%) of cold-tolerant isolates exhibited putative ACC deaminase activity. Significant (p \u2264 0.05) differences in IRI activity were also observed between the studied plant niches. Surprisingly, all the cold-tolerant isolates showed ACC deaminase activity, independent of the plant niches, with 12 isolates showing the highest ACC deaminase activities of 13.21\u201339.56 mmol \u03b1 KB mg protein\u22121 h\u22121. These isolates were categorized as \u2018cold-tolerant hyper-ACC-degrading bacteria\u2019, and identified as members of Pseudomonas, Serratia, and Staphylococcus genera. The results revealed the occurrence of cold-tolerant hyper-ACC-degrading bacteria in diverse plant niches of Antarctic vascular plants, that could be investigated as novel microbial inoculants to alleviate abiotic stresses in plants.<\/jats:p>","DOI":"10.3390\/microorganisms8111788","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T11:04:20Z","timestamp":1605524660000},"page":"1788","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants"],"prefix":"10.3390","volume":"8","author":[{"given":"Macarena A.","family":"Araya","sequence":"first","affiliation":[{"name":"Laboratorio de Ecolog\u00eda Microbiana Aplicada (EMALAB), Departamento de Ciencias Qu\u00edmicas y Recursos Naturales, Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Valenzuela","sequence":"additional","affiliation":[{"name":"Laboratorio de Ecolog\u00eda Microbiana Aplicada (EMALAB), Departamento de Ciencias Qu\u00edmicas y Recursos Naturales, Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitza G.","family":"Inostroza","sequence":"additional","affiliation":[{"name":"Laboratorio de Ecolog\u00eda Microbiana Aplicada (EMALAB), Departamento de Ciencias Qu\u00edmicas y Recursos Naturales, Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"},{"name":"The Network for Extreme Environment Research (NEXER), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"},{"name":"Center of Plant- Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2347-616X","authenticated-orcid":false,"given":"Fumito","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Laboratorio de Ecolog\u00eda Microbiana Aplicada (EMALAB), Departamento de Ciencias Qu\u00edmicas y Recursos Naturales, Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"},{"name":"Center of Plant- Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"},{"name":"Microbial Genomics and Ecology, Office of Industry-Academia-Government and Community Collaboration, Hiroshima University, Hiroshima 739-8529, Japan"},{"name":"Center for Holobiome and Built Environment (CHOBE), Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4760-6379","authenticated-orcid":false,"given":"Milko A.","family":"Jorquera","sequence":"additional","affiliation":[{"name":"Laboratorio de Ecolog\u00eda Microbiana Aplicada (EMALAB), Departamento de Ciencias Qu\u00edmicas y Recursos Naturales, Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"},{"name":"The Network for Extreme Environment Research (NEXER), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"},{"name":"Center of Plant- Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"},{"name":"Center for Holobiome and Built Environment (CHOBE), Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7752-166X","authenticated-orcid":false,"given":"Jacquelinne J.","family":"Acu\u00f1a","sequence":"additional","affiliation":[{"name":"Laboratorio de Ecolog\u00eda Microbiana Aplicada (EMALAB), Departamento de Ciencias Qu\u00edmicas y Recursos Naturales, Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"},{"name":"The Network for Extreme Environment Research (NEXER), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"},{"name":"Center of Plant- Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Ave. Francisco Salazar, 01145 Temuco, Chile"},{"name":"Center for Holobiome and Built Environment (CHOBE), Hiroshima University, Hiroshima 739-8529, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,14]]},"reference":[{"key":"ref_1","unstructured":"FAO (2019). 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