{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T18:45:18Z","timestamp":1775760318480,"version":"3.50.1"},"reference-count":22,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T00:00:00Z","timestamp":1606176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005969","name":"Universit\u00e0 di Bologna","doi-asserted-by":"publisher","award":["RFO 2016-2019"],"award-info":[{"award-number":["RFO 2016-2019"]}],"id":[{"id":"10.13039\/501100005969","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009879","name":"Regione Emilia-Romagna","doi-asserted-by":"publisher","award":["Lab P3"],"award-info":[{"award-number":["Lab P3"]}],"id":[{"id":"10.13039\/501100009879","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>This study compares the performance of seven assays, including two ELISA (Leishmania ELISA IgG + IgM, Vircell Microbiologists; Leishmania infantum IgG ELISA, NovaTec), three rK39-based immunochromatographic tests (rK39-ICTs) (Leishmania Dipstick Rapydtest, Apacor; On Site Leishmania IgG\/IgM Combo Rapid Test, CTK Biotech; LEISHMANIA Strip quick Test, Cypress Diagnostic), one indirect immunofluorescent antibody test (IFAT) (Leishmania-Spot IF, BioM\u00e9rieux), and one western blot (WB) (Leishmania WESTERN BLOT IgG, LDBio Diagnostics) for serodiagnosis of visceral leishmaniasis (VL). Serum samples from 27 VL patients living in northeastern Italy were analyzed, as well as the serum samples from 50 individuals in whom VL diagnosis was excluded. The WB and the IFAT had 96% sensitivity, followed by the ELISA (63% and 74%, respectively). The rK39-ICT exhibited the worst performance among the serological tests, with sensitivities ranging from 52% to 70%. By combining selected ELISA\/ICT, the sensitivity of VL detection reached 89%. IFAT and WB outperformed ELISA and rK39-ICT by possessing optimal sensitivity, but their high cost and complexity of execution would not allow their employment as screening tests. In conclusion, the combination of easy-to-perform tests, such as ICT and ELISA, could improve sensitivity in the serodiagnosis of Mediterranean VL.<\/jats:p>","DOI":"10.3390\/microorganisms8121847","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T09:06:28Z","timestamp":1606208788000},"page":"1847","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Serodiagnosis of Visceral Leishmaniasis in Northeastern Italy: Evaluation of Seven Serological Tests"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5447-8817","authenticated-orcid":false,"given":"Margherita","family":"Ortalli","sequence":"first","affiliation":[{"name":"Unit of Microbiology, Regional Reference Center for Microbiological Emergencies (CRREM), St. Orsola Malpighi Hospital, University of Bologna, 40100 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniele","family":"Lorrai","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40100 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Gaibani","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Regional Reference Center for Microbiological Emergencies (CRREM), St. Orsola Malpighi Hospital, University of Bologna, 40100 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giada","family":"Rossini","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Regional Reference Center for Microbiological Emergencies (CRREM), St. Orsola Malpighi Hospital, University of Bologna, 40100 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caterina","family":"Vocale","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Regional Reference Center for Microbiological Emergencies (CRREM), St. Orsola Malpighi Hospital, University of Bologna, 40100 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Carla","family":"Re","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Regional Reference Center for Microbiological Emergencies (CRREM), St. Orsola Malpighi Hospital, University of Bologna, 40100 Bologna, Italy"},{"name":"Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40100 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0862-4937","authenticated-orcid":false,"given":"Stefania","family":"Varani","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Regional Reference Center for Microbiological Emergencies (CRREM), St. Orsola Malpighi Hospital, University of Bologna, 40100 Bologna, Italy"},{"name":"Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40100 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,24]]},"reference":[{"key":"ref_1","unstructured":"WHO (2020, October 24). 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